in river water using multiplex pcr

Annual and Diurnal Profiles of Cryptosporidium and Giardia in River Water in Japan

Ngày tải lên : 05/09/2013, 10:17

... monitoring of protozoans was performed in tributary rivers of the Tone River basin in Japan where the surface water is utilized for the main production of drinking water for people living in the ... closely on the discharge point of treated sewage wastewater Sampling sites for investigating annual profiles Sampling sites for investigating diurnal profiles River flow Tone River Site A Site B Tone ... Giardia in river water - 228 - Journal of Water and Environment Technology, Vol 9, No.2, 2011 The protozoan concentration in main rivers increased after the confluence of tributary rivers, which indicates...

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Biological Alteration of Zinc Complexation Characteristics of Dissolved Organic Matter in Domestic Wastewater Treatment Plant Effluent under River Water Environment

Ngày tải lên : 05/09/2013, 10:15

... of zinc binding sites in river water DOM Fig represents the variation of DOC, UV254 and SUVA during the river water incubation for weeks in the preliminary study During the first days of incubation, ... shown in Fig and Table 1, Zn titration data obtained for DOM in the original river water sample seems to fit into two linear portions indicating two classes of binding sites In contrast, DOMs in ... Biodegradability of zinc binding sites in DOM of WWTP effluent in river water Fig shows the variation of DOC, UV254 and SUVA during the incubation of DOM in WWTP effluent with river water, where river water...

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Production of hydrogen using composite membrane in PEM water electrolysis

Ngày tải lên : 05/09/2013, 16:11

... organic impurities • Rinsing with boiling water for 2h • Boiling in H2SO4 for 1h to remove ionic impurities • And finally the composite membrane is washed with deionized water to remove any excess ... IEC value (0.96 meq g-1) in comparison to that of Nafion 115 Membrane (0.84meq g-1) by using (Eq: 1), indicating an active contribution of the synthesized fillers in providing free acid groups to ... hydrogen yield in a single cell proton exchange membrane (PEM) water electrolysis Int J Hydrogen Energy 2009,34, 6085 – 6088 ISSN 2076-2895 (Print), ISSN 2076-2909 (Online) ©2012 International...

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Tài liệu WATER POLLUTION BY INSECTICIDES IN AN AGRICULTURAL RIVER .BASIN. I. OCCURRENCE OF INSECTICIDES IN RIVER AND TREATED MUNICIPAL WATER pdf

Ngày tải lên : 18/02/2014, 04:20

... organic contaminants in the nation’s rivers Ind Eng IN A RIVER: TIIE WATER 317 Chem., 52 ( 6) : 99A-102A 1959 ROSEN, A A., AND F M MIDDLETON Chlorinated insecticides in surface waters Anal Chcm., ... Rainfall, stream turbidity, and insccticidc usage data were obtained to determint their influence on the quantities of insecticides recovcrcd from the samples Daily rainfall records were obtained ... was obtained by personal interview Confidence limits (0.05) were set on the estimates by using the sample TABLE Estimales of insecticide use and cotton ureas treated in the Flint Creek Basin TABLE...

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Visitor Profiles, Economic Impacts and Recreational Aesthetic Values Associated with Eight Priority Florida Springs Located in the St. Johns River Water Management District pot

Ngày tải lên : 08/03/2014, 08:20

... Springs Seasonality in Lake County is used for Apopka Spring and Bugg Spring in same county; ***** Gemini Springs Seasonality in Volusia County is used for Green Springs in same county FINDINGS ... St Johns River Recreational activities at Alexander Springs include camping, swimming, scuba diving, hiking, fishing, bicycling, and canoeing The U.S National Park Service owns these springs Private ... greatly within the year Rainfall in Florida varies considerably depending on the region of the state For example, precipitation in Jacksonville in 2001 was 0.6 inches in April and 16 inches in September...

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Báo cáo " Assessment of OPRM1 and HRH2 gene variants in Vietnamese using RFLP-PCR " ppt

Ngày tải lên : 22/03/2014, 09:20

... tissues including the gastrointestinal tract, the immune system and the brain [5] It is a central neurotransmitter in the brain and is formed in the posterior hypothalamus from exogeneous histidine ... associated with schizophrenia in British Caucasians [6] In the present study, we applied PCR- RFLP in investigating the frequency of such SNPs in Vietnamese subjects as our preliminary assessment [7] For ... Val at position 40 instead of Ala, have been found with varied frequencies across populations These findings demonstrated that the opioid system is involved in the reinforcing properties of alcohol...

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Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx

Ngày tải lên : 07/08/2014, 18:21

... Antimicrobial discs Kanamycin Levofloxacin Nalidixic acid Norfloxacin Ofloxacin Polymyxin B Sparfloxacin Streptomycin Tetracycline Tobramycin Trimethoprim/ sulfamethoxazole Resistant (%) Intermediate (%) ... strategy against STEC infection in humans and animals, and food contamination in livestock products Materials and Methods Bacterial strains E coli O157:H7 strains used in this study are listed in Table ... obtained from chicken meat processing plants and markets by rinsing the samples with buffered peptone water (BPW; Becton Dickinson, USA) In case of fecal samples, a cup of feces was taken into...

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Rapid detection of epidermal growth factor receptor mutations with multiplex PCR and primer extension in lung cancer pdf

Ngày tải lên : 10/08/2014, 05:21

... of the EGFR genes in a single tube The primers and conditions used for the multiplex PCR were Page of the same as the PCR described above After multiplex PCR amplification, the PCR products were ... of the primer extension reaction is its multiplexing capability, with several mutations being detected in a single reaction tube Multiplex PCR- SSCP- or PCRARMS-based methods can also simultaneously ... purified to remove the remaining primers and unincorporated deoxynucleotide triphosphates, using the PCR- M™ Clean Up System (Viogene-biotek Co., Sunnyvale, CA, USA) After removing the primers, the...

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Báo cáo y học: "Detection of poliovirus by ICC/qPCR in concentrated water samples has greater sensitivity and is less costly using BGM cells in suspension as compared to monolayers" pot

Ngày tải lên : 12/08/2014, 01:22

... detecting viruses in water Presented at 2006 EPA Forum Washington, DC 2006 Choi S, Jiang SC: Real time PCR quantification of human adenoviruses in urban rivers indicates genome prevalence but low infectivity ... by Goldstein et al proved that cells in a true suspension or 3d configuration aids in poliovirus (and other viruses) infection In a clinical setting Luker et al demonstrated that even in low numbers ... PV1 infection in cells that were in suspension compared to the monolayer conformation Trypsinization immediately before the suspensions were inoculated may have increased yield however Goldstein...

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báo cáo khoa học: " Profiling microRNA expression in Arabidopsis pollen using microRNA array and real-time PCR" pps

Ngày tải lên : 12/08/2014, 03:20

... control in RTPCR ACTIN7 is strongly expressed in vegetative tissues, but not expressed in pollen [21] No product was detected for ACTIN7 in our RNA samples using the same number of cycles in PCR ... with those in Genes in RNA silencing pathway are expressed in mature pollen Our lack of knowledge of miRNA functioning in mature pollen may be explained by inactivation of the RNA silencing pathway ... low in abundance, we think that the miRCURY array may not be sensitive enough to detect their expression To further confirm our findings, we examined the expression of 27 miRNAs by using RT-PCR...

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Báo cáo khoa học: " Respiratory viral infections detected by multiplex PCR among pediatric patients with lower respiratory tract infections seen at an urban hospital in Delhi from 2005 to 2007" docx

Ngày tải lên : 12/08/2014, 04:22

... presence of inhibitors of the RT -PCR assay Sensitivity and specificity of the Multiplex PCR The sensitivity of the multiplex PCR assay was determined by TCID50 using Reed and Muench method [43] Inter ... shows detection of single and mixed infections in some samples on which two tube multiplex PCR was applied Of these, 86 were single virus infections and mixed infections were seen in 20 children (18.8% ... by multiplex PCR Variables Median age (Mo) Sex M:F Clinical symptoms Cough Difficulty in breathing Runny nose Sore throat Fever Hoarseness Asthma Grunting Nasal flaring Stridor Chest indrawing...

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Assessing Water Availability in PoKo Catchment using SWAT model

Ngày tải lên : 13/08/2015, 10:01

... Association of Soil and Water Conservation, USA Liem, N D, N T Hong, T P Minh, and N K Loi 2011 Assessing Water Discharge in Be River Basin, Vietnam using GIS and SWAT model In: Proceeding of the National ... considerably fluctuations in total water discharge Water yield 3000 Rainfall เชิงเส้ น (Water yield) เชิงเส้ น (Rainfall) Water yield Rainfall เชิงเส้ น (Water yield) เชิงเส้ น (Rainfall) 3000 2500 y ... coming years of Kon availability in NSIbalance diagramthisalso one of the keyin the is to the coming years province.PoKoprovince as well as of the water tools to more sustainable in the coming...

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Target detection in bubble populated water using bio mimetic sonar

Ngày tải lên : 16/10/2015, 11:57

... nonlinear phenomena in single bubbles They found that with increase in amplitude of the driving pressure, single bubbles can be driven into nonlinear oscillations resulting in harmonic dispersions ... Pacific spotted dolphin, spinner dolphin, pilot whale, rough tooth dolphin and Chinese river dolphin Species that fall under the second category include the harbor porpoise, finless porpoise, Dall’s ... wakes, living or decomposing organisms, and wave breaking; the latter being the dominant cause of bubble entrainment in the surf zone Despite the complications of sound propagation in bubble...

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Carrier detection in families affected by duchenne muscular dystrophy using multiplex ligation dependent probe amplification (1)

Ngày tải lên : 05/11/2015, 17:38

... roddomain • The cystein- rich domain • The carboxy- terminal domain 11 The first domain is the amino-terminal domain It has homology with αactin and contains between 232 and 240 amino acid ... based on the principle of PCR Among these methods, multiplex PCR is appreciated in the diagnosis of deletion and about 98% of deletions are easily detectable using multiplex PCR in affected males ... B: protein products of the DMD gene [35] 1.3 Protein dystrophin The product of the dystrophin gene is dystrophin protein A complete understanding about the functions of protein dystrophin is important...

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Carrier detection in families affected by duchenne muscular dystrophy using multiplex ligation dependent probe amplification (2)

Ngày tải lên : 05/11/2015, 17:39

... muscle cells and is divided into four domains [23]: the amino-terminal domain, the central- roddomain, the cystein- rich domain, he carboxy- terminal domain Dystrophin plays an important structural ... important role in the process of mutation detection For example, multiplex PCR is appreciated in the diagnosis of deletion and about 98% of deletions are easily detectable using multiplex PCR in affected ... corresponding PCR tube Spin - Prepare PCR master mix (in icebox) 12 - Continue the thermocycler program: pause at 60 °C and place the PCR tubes in the thermocycler - While these tubes are in a thermocycler...

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Expert Service Oriented Architecture in C Sharp Using the Web Services Enhancements

Ngày tải lên : 20/08/2012, 13:59

... working with Indigo in the future This chapter contains the following: • Overview of Indigo architecture, including the Indigo service layer, the Indigo connector, hosting environments, messaging ... supports, including data type information, and binding information for locating and communicating with the Web service operations • Services provide endpoints that consumers and other services can bind ... implementing industry-standard Web service specifications, including WS-Security: A wide-ranging specification that integrates a set of popular security technologies, including digital signing and...

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Xác định giới tính bằng kỹ thuật multiplex pcr trên ba giống bò

Ngày tải lên : 29/10/2012, 14:13

... M., 1990 Sex determination of bovine embryos using the polymerase chain reaction Animal Biotechnology 1(2): 121-133 28.Takara Bio Inc CycleavePCRTM Bovine Sexing Kit Ho Chi Minh city, Viet Nam, ... Protein vân chủ yếu actin, myosin myoglobin Trong đó, myoglobin loại protein thuộc nhóm chromoprotein giúp tạo màu đỏ bắp thịt (Nguyễn Phước Nhuận ctv, 2003) Myoglobin cấu tạo từ tiểu đơn vị globin ... Thường có dạng keratin α-keratin β-keratin α-keratin có cấu trúc vòng với nhiều liên kết cystine Trong β-keratin có cấu trúc phiến xếp nếp Nét đặc biệt keratin hàm lượng cystine cao nên mạch polypeptide...

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ứng dụng kỹ thuật multiplex – pcr để phát hiện một số gen độc lực của nhóm escherichia caoli sản sinh độc tố shiga (stec) phân lập được từ phân bò, heo và thịt bò

Ngày tải lên : 29/10/2012, 14:23

... diarrhea stools samples of bovines, calves, piglets, and swabs of the beef surface by multiplex - PCR There was the observing on each the isolating medium: one kind of pink colony on MAC (HM), white ... bề mặt thịt SUMMARY iv “The application of multiplex - PCR in detecting some virulent genes of E coli isolated from diarrhea faeces of bovine and swine, swabs of beef surface” The study was carried ... coli carrying stx, including of of samples were detected E coli carrying stx1 (25%) and of samples were detected E coli carrying stx2 (75%); all of samples (100%) were detected E coli carrying ehxA,...

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Ứng dụng kỹ thuật multiplex – pcr để phát hiện các gen độc lực của vi khuẩn escherichia coli phân lập từ phân bò, phân heo tiêu chảy và thịt bò

Ngày tải lên : 29/10/2012, 14:23

... Đó intimin - loại protein có trọng lượng phân tử 94 – 97 kDa Protein intimin mã hóa gen eae (E coli attaching effacing) Intimin gây tổn thương dạng bám dính phá hủy (attaching - and – effacing, ... enterotoxin II Infect Immum 60:4468-4474 30 Karmali M A.,1989 Infection by verocytotoxin – producing Escherichia coli J Clin Microbiol Rev 2:15-38 31 Kenny B., DeVinney R., Stein M., Reinscheid ... phân lập multiplex - PCR - Việc phát gen độc lực E coli thực phản ứng multiplex – PCR với máy ln nhiệt (thermal cycler):  Multiplex – PCR1 : Phát gen eae, hly, stx1, stx2  Multiplex – PCR2 : Phát...

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ứng dụng kỹ thuật multiplex – pcr để phát hiện một số gen độc lực của nhóm escherichia coli sản sinh độc tố shiga (stec) phân lập được từ phân bò, heo tiêu chảy và thịt bò

Ngày tải lên : 31/10/2012, 09:07

... ruột Đó intimin, protein màng có trọng lượng phân tử 94-97 kDa Intimin mã hóa gen eae (E coli attaching and effacing) Intimin gây tổn thương dạng bám dính phá hủy, gọi bệnh tích A/E (attaching and ... nhiều ứng dụng sinh học, y khoa, nông nghiệp, khảo cổ, pháp y hình Trong nghiên cứu genome, PCR ứng dụng nhân vô tính, multiplex PCR, cloning cDNA PCR đảo (inverse PCR) , recombinant PCR, Trong y ... Escherichia coli in intimate attachment in vitro and in a porcine model J Clin Invest 92: 1418 - 1424 17.Doyle M P., 1991 Escherichia coli O157:H7 and its significance in foods International J...

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