immune modulation by tcdd and related polyhalogenated aromatic hydrocarbons
Immune modulation by experimental filarial infection and its impact on e coli induced sepsis
... binding a receptor complex formed by TLR2, TLR6, and CD14, and signaling via MyD88 and Mal TLR1, TLR4, TRAM and TRIF are not required for Wolbachia-induced immune responses (Hise et al., 2007) ... 2001, 2008; Mand et al., 2009; Taylor et al., 2014; Volkmann et al., 2003a) 1.2.4 Effects of helminth-induced immune modulation on bystander responses Helminth infections induce type immune responses ... transcription by IL-4 and LPS (El Chartouni and Rehli, 2010; Gordon and Martinez, 2010; Mosser and Edwards, 2008; Xue et al., 2014), however, induction of endotoxin tolerance may promote an AAM-related...
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Chapter 131. Diphtheria and Other Infections Caused by Corynebacteria and Related Species (Part 6) doc
... pharynx and skin C xerosis is found on the skin, nasopharynx, and conjunctiva; C auris in the external auditory canal; and C striatum in the anterior nares and on the skin C jeikeium and C urealyticum ... organism causes a diphtheria like illness and produces both diphtheria toxin and a dermonecrotic toxin C ulcerans is a commensal in horses and cattle and has been isolated from cow's milk The ... antitoxin and antibiotics should be initiated when respiratory C ulcerans is identified, and a contact investigation (including throat cultures to determine the need for antimicrobial prophylaxis and...
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Chapter 131. Diphtheria and Other Infections Caused by Corynebacteria and Related Species (Part 7) pdf
... belongs to CDC Group ANF-1 and has been isolated from human blood and abscess infections C accolens has been isolated from wound drainage, throat swabs, and sputum and is typically identified ... flora and does not produce diphtheria toxin C propinquum, a close relative of C pseudodiphtheriticum, is part of CDC Group ANF-3 and is isolated from human respiratory tract specimens and blood ... erythropolis, R rhodochrous, and R rubropertinctus R equi has been recognized as a cause of pneumonia in horses since the 1920s; it causes related infections in cattle, sheep, and swine R equi is found...
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Chapter 131. Diphtheria and Other Infections Caused by Corynebacteria and Related Species (Part 1) pptx
... 10 were immune to C diphtheriae; infants were protected by maternal IgG antibodies but became susceptible after ~6 months of age Thus, the disease was seen primarily in children and nonimmune ... frequent vaccine shortages, delays in implementation of vaccination and of treatment in response to cases, and lack of public education and awareness were contributing factors in that outbreak Cutaneous ... the independent states formerly encompassed by the Soviet Union Additional outbreaks have been reported in Algeria, China, and Ecuador Etiology C diphtheriae is a gram-positive, unencapsulated,...
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Chapter 131. Diphtheria and Other Infections Caused by Corynebacteria and Related Species (Part 3) ppsx
... of the submandibular and paratracheal region and is further characterized by foul breath, thick speech, and stridorous breathing The diphtheritic pseudomembrane is gray or whitish and sharply ... larynx, nares, and trachea or bronchial passages Large pseudomembranes are associated with severe disease and a poor prognosis A few patients develop massive swelling of the tonsils and present ... headache, and voice change are the initial manifestations Neck edema and difficulty breathing are seen in more advanced cases and carry a poor prognosis The systemic...
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Chapter 131. Diphtheria and Other Infections Caused by Corynebacteria and Related Species (Part 4) ppsx
... investigational new drug protocol and may be obtained by calling the Bacterial Vaccine Preventable Disease Branch of the National Immunization Program at 404-639-8257 between 8:00 A.M and 4:30 P.M U.S Eastern ... resolution of fever and a lower rate of bacterial resistance than erythromycin; however, relapses were more common with penicillin Erythromycin therapy targets protein synthesis and thus offers the ... cutaneous diphtheria requires laboratory confirmation since the lesions are not characteristic and are clinically indistinguishable from other dermatoses Diphtheritic ulcers occasionally—but...
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Chapter 131. Diphtheria and Other Infections Caused by Corynebacteria and Related Species (Part 5) docx
... diphtheria and tetanus toxoids and acellular pertussis vaccine, adsorbed) is the currently recommended vaccine for children up to the age of 7; DTaP replaced DTP (diphtheria and tetanus toxoids and ... toxoid, reduced diphtheria toxoid, and acellular pertussis vaccine formulated for adolescents and adults Tdap was licensed for use in the United States in 2005 and is the recommended booster vaccine ... vaccine Carriers of C diphtheriae in the community should be treated and vaccinated when identified Nondiphtherial Corynebacteria and Related Species Nondiphtherial corynebacteria, which are also referred...
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Báo cáo lâm nghiệp: "Leaf morphology as species indicator in seedlings of Quercus robur L. and Q. petraea (Matt.) Liebl.: modulation by irradiance and growth flush" potx
... thickness [2] under shade, Blue and Jensen [5] indicated that sun leaves of oak (Q velutina, Q rubra and Q palustris) had larger and deeper sinuses, a narrower blade and a higher number of veins ... MASS / AREA (g·cm–2) MATERIAL AND METHODS 2.1 Plant material and experimental design Acorns were collected during autumn 1997 in two adjacent pure stands of Q petraea and Q robur in the forest of ... leaf morphology assessment is described by Dupouey and Badeau [8] Twenty-three variables were measured and used to derive 11 calculated variables (Tab I and Fig 1) These variables concern various...
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Báo cáo khoa học: Typical 2-Cys peroxiredoxins – modulation by covalent transformations and noncovalent interactions doc
... participation of ATP in covalent transformations of (a) Thr90 catalyzed by CDK1, (b) CysR by autophosphorylation and (c) CysP assisted by Srx, and noncovalent interactions that drive the quaternary structure ... modification of thiols by reactive oxygen and nitrogen species Trends Biochem Sci 27, 489–492 Allen JF (1993) Control of gene expression by redox potential and the requirement for chloroplast and mitochondrial ... substrate and (b) the differential regulation of peroxidase and chaperone activities by modulators devoid of redox capacity In contrast with the phosphorylation of human 2-Cys Prx mediated by the...
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Báo cáo khoa học: Oligosaccharide synthesis in Fibrobacter succinogenes S85 and its modulation by the substrate potx
... incubation were taken at 1, and h, and extracellular and intracellular media were separated by centrifugation and analysed in parallel by 1H NMR spectroscopy and by TLC Under these conditions, ... procedures) were separated by centrifugation and analysed, in parallel, by 1H NMR spectroscopy and by TLC Consumption of the substrates and production of the final metabolites (succinate and acetate) were ... cellulose and with cellobiose (the main product of cellulolysis), and with a mixture of glucose and cellobiose Cell extracts and extracellular media were analysed by 2D NMR spectroscopy and by TLC...
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Báo cáo khoa học: Pherokine-2 and -3 Two Drosophila molecules related to pheromone/odor-binding proteins induced by viral and bacterial infections ppt
... analyzed by HPLC and the molecule induced by the viral infection was detected by MALDI-TOF MS (Fig 2B) The molecule was purified to homogeneity by gel permeation and reversed-phase chromatography and ... containing two layers of caesium chloride (5% and 40%), and ultracentrifuged for 16 h at 36 000 g at 15 °C The virus band was collected and DCV was recovered by centrifugation (25 000 g, h 30 min, 15 ... gradient and analyzed by electron microscopy as described [22] Briefly, 4000 flies were injected with DCV and collected and frozen after death Flies were crushed in 10 mM Tris/HCl (pH 7.5), followed by...
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DNA Replication and Related Cellular Processes Edited by Jelena Kušić - Tišma pdf
... and C) and nuclei in the intestine of an early L4 larva (A’, B’, and C’) are indicated by arrows Panels show DNA staining by DAPI (A and A’), EdU staining (B and B’) and merged images (C and C’) ... leading-strand, using the 3′ end that was generated by resection, and that Rad51-mediated strand invasion is used as the primer end with primase activity On the other hand, BIR require laggingstrand ... important antigens related to cell cycle were discovered later including PCNA, KiS2 and MCM Ki67 antigen was discovered by a German group of scientists (2) in early 1980s and identified by using mice...
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Báo cáo khoa học: Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid pdf
... into SFC and FFC, SFD, FFD and FFD-500 groups SFC and SFD groups received AIN-76 basal diet, whereas FFC and FFD groups received AIN-76 diet, wherein starch was replaced with 5% wheat bran and 2.5% ... SDS/PAGE of laminin The purity of laminin was ascertained by 3.5% SDS ⁄ PAGE and visualized by silver staining for bands corresponding to 400 and 200 kDa FEBS Journal 278 (2011) 143–155 ª 2010 The ... anhydride and pyridine (1 : 1, mL) and processed further to remove the salts The derivatized products were taken in chloroform and analyzed by GLC The conditions used for the analysis of neutral and...
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Báo cáo khoa học: Modulation of heme and myristate binding to human serum albumin by anti-HIV drugs An optical and NMR spectroscopic study potx
... showing that all FA7 ligands reduce the affinity of heme by one order of magnitude and, Fig Superimposition of Fe(III)heme and abacavir (A) and nevirapine (B) in binding site FA1 Ligands are coloured ... their ligands and potentially acting as an allosteric effector By contrast, atazanavir (Fig 5C) could partially enter the binding cavities, although steric clashes between this large ligand and the ... 4493 Modulation of HSA ligand binding by anti-HIV drugs G Fanali et al Fig Binding isotherms for Fe(III)heme binding to HSA (FA free) and to HSA–myristate complexes in the absence of drugs (A) and...
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Báo cáo khoa học: Interaction of ostreolysin, a cytolytic protein from the edible mushroom Pleurotus ostreatus, with lipid membranes and modulation by lysophospholipids pptx
... lysophospholipids and fatty acids, was conrmed by standard TLC and electrospray ionization mass spectroscopy Ammodytin I2 alone did not affect ostreolysin-induced hemolysis, and was not hemolytic by itself, ... we observed a strong and specic inhibition by a series of lysophospholipids, in particular lysophophatidylinositol and sphingosine-1-phosphate, and, to a lesser degree, by nonesteried fatty acids ... b-sheet; band at 1651 cm)1, a-helix; band at 1640 cm)1, random coil; bands in the region 16381616 cm)1, b-sheet (parallel plus antiparallel) These were used to curve t the original spectrum, and...
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Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting docx
... water The animals were killed by cervical dislocation, and tibialis anterior, gastrocnemius and soleus muscles were carefully dissected and kept on ice In fasted animals and the respective controls, ... containing 0.1% Tween and hybridized with a : 1000 diluted goat anti-rabbit peroxidase-labeled secondary antibody The signals were detected by chemiluminescence using a standard ECL kit and developed ... concentrations of the experimental samples were calculated by comparison with the standard curves Background fluorescence was removed by setting a noise band The number of cycles at which the best-fit line...
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Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf
... after each treatment was tested by Trypan blue dye exclusion, and was found to be higher than 90% in all cases Materials and methods Anandamide hydrolase activity and expression Materials Chemicals ... Fig Effect of leptin and progesterone on FAAH activity and expression (A) Effect of leptin on the activity of FAAH in human U937 and CHP100 cells and on the protein content and the mRNA of FAAH ... Fig Analysis of FAAH promoter by transient expression (A) Proximal upstream region of human FAAH gene Left and right handed arrows denote SP1 sites in the – and + strands, respectively Pentagonal...
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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx
... extracted, reverse transcribed and amplified by qRT-PCR in the presence of primers and probes specific for the indicated genes and β-actin house keeping gene (panels A-F) Cytokine and chemokine gene expression ... Glutamax and non-essential amino acids (all from GIBCO Paisley, Scotland), thereafter referred to as complete medium, and 5% (v/v) human serum (Blutspendezentrum, University Hospital Basel, Switzerland) ... supplemented with μl 10 mM dNTP mix, μl 5× first-strand buffer, μl 0.1 M DTT and μl (200 units) M-MLV reverse transcriptase (all by Invitrogen Ltd., Paisley, UK) and incubated at 37°C for hour Two μl of...
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Báo cáo y học: "Negative regulation of cytokine signaling and immune responses by SOCS proteins" potx
... recruit and activate STATs (especially STAT5) has been proposed as the mechanism by which CIS and SOCS2 inhibit cytokine signaling [11,12] CIS is induced by cytokines that activate STAT5 and bind ... with control siRNA, as indicated by an enhanced secretion of proinflammatory cytokines such as IL6 and TNF-α and by the enhanced phosphorylation of STAT1, IκB and JNK upon stimulation Antigen ... possible, and also improved anti-tumor immunity [69] A decrease in SOCS1 and SOCS3 by antisense RNA treatment in obese diabetic mice improved insulin sensitivity and ameliorated hepatic steatosis and...
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Báo cáo y học: "The gene expression profile of preclinical autoimmune arthritis and its modulation by a tolerogenic disease-protective antigenic challeng" ppt
... autoimmune arthritis and its modulation by a tolerogenic disease-protective antigenic challenge Hua Yu1, Changwan Lu2,3, Ming T Tan3 and Kamal D Moudgil1,4,* Department of Microbiology and Immunology, ... homogeneity and controlled disease induction, AA is an appropriate model system to examine early pathogenetic events of autoimmune arthritis and their modulation by therapeutic regimen, including immune- based ... adaptive immune response and effector functions Stress protein -related and other autoantigens, antigen processing and presentation, T cell costimulation, cytokines, cytokine receptors, activators and...
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