identification of subpopulations of cd4 and cd8 t cells that differ in function
Báo cáo hóa học: " Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients" pdf
... indicating the reduction of recent thymic emigrants affects the majority of TRBV subfamilies Page of Competing interests The authors declare that they have no competing interests Authors' contributions ... sjTRECs in sorted CD4+ and CD8+ T cells This is the most sensitive and accurate method for quantitation of naïve T- cells It allows also the comparison of sjTRECs levels in CD4+ and CD8+ subsets The ... cases, and CD4+ and CD8+ T cells from 19 cases TRBV sjTRECs were determined in PBMCs, CD4+ and CD8+ T Quantitative detection of δRec-ψJα sjTRECs in DNA from PBMCs and sorted CD4+ or CD8+ T cells...
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Báo cáo khoa học: "Density of CD4(+) and CD8(+) T lymphocytes in biopsy samples can be a predictor of pathological response to chemoradiotherapy (CRT) for rectal cancer" ppt
... attracts T cells [28] In a previous study, we found that the circulating lymphocyte count is correlated with tumor response to CRT [13] This finding is in line with the data in this study, and ... density of T cells infiltrating in solid tumor and response to CRT On the other hand, Grabenbauer et al previously reported that tumor-infiltrating CD3(+) T cells, especially granzymeB(+) CD8( +) T ... a strong correlation with tumor response to CRT, indicating that tumors attracting T cells are more liable to respond to CRT Many previous reports have suggested that a high number of TIL in...
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Báo cáo y học: " Antibody microarray analysis of cell surface antigens on CD4+ and CD8+ T cells from HIV+ individuals correlates with disease stages" ppt
... establishes the location of each dot on the array and also served as the internal control to measure the distribution of cells The dot pattern obtained is the immunophenotype of that population of leukocytes ... that the use of this technology will facilitate further investigation of the causes and control of HIV disease progression and eventually lead to a better understanding of the pathogenesis of the ... http://www.retrovirology.com/content/4/1/83 Figure Composite dot scan patterns of antibody binding for CD4+ T cells Composite dot scan patterns of antibody binding for CD4+ T cells Half of a duplicate array was shown with the...
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Báo cáo y học: "Isolated receptor binding domains of HTLV-1 and HTLV-2 envelopes bind Glut-1 on activated CD4+ and CD8+ T cells" pot
... Glut-1 that is not present at the cell surface In support of the latter hypothesis, Baldwin and colleagues have elegantly shown that cytokines and other growth signals induce the translocation of ... of CD8 T lymphocytes These data are in complete contradiction with the longstanding notions that; 1) quiescent T cells demonstrate very low glucose transport and 2) the energy demands of an activated ... CD4 and CD8 lymphocytes: Induction of H1RBD and TCR stimulation binding TCR stimulation results in Glut-1 expression and concomitant glucose uptake in CD4 and CD8 lymphocytes: Induction of H1RBD...
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Báo cáo y học: "Heterogeneity of CD4+ and CD8+ memory T cells in localized and generalized Wegener’s granulomatosis." potx
... on CD4+ CD45RO+ and CD8 +CD45 RO+ and also on CD4+ CD45RA+ and CD8 +CD45 RA+ T cells indicates activation and the potential to respond to chemotactic gradients in inflammatory areas, which is consistent ... described Thus, the local cytokine milieu might depend on the site and extent of disease activity [4,5] The shift in the cytokine profile in granulomatous lesions of the respiratory tract might be of ... functional aspects such as response in vitro to chemotactic gradients, cytokine release and cytotoxic activity of distinct T cell populations Detection of the inducible inflammatory chemokine...
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Báo cáo y học: "Genome-wide analysis of primary CD4+ and CD8+ T cell transcriptomes shows evidence for a network of enriched pathways associated with HIV disease" pot
... GBP1R tccaggagtcattctggttgt BDLvsVIR CD8 -2.5 -2.4 ACTA2 NM_001613.1 actin, alpha 2, smooth muscle, aorta ACTA2L ctgttccagccatccttcat ACTA2R tcatgatgctgttgtaggtggt BDLvsVIR CD8 -1.3 -2.2 ATP6V1D ... NM_015994.2 ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D ATP6V1DL ttttcactagctgaagccaagtt ATP6V1DR gcgctttattgacattttggat VIRvsLTNP CD8 2.0 2.8 BAG3 cagccagataaacagtgtggac BAG3R agaggcagctggagactgg ... non-cytotoxic CD8+ T cell activity and comparisons between individuals with high non-cytotoxic activity and uninfected controls [17,18] Recently, the transcriptional profiling of CD4+ and CD8+ T cells...
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Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
... corrected quantity of CD4 and CD8 mRNA as determined by QC-RT-PCR and the corresponding CD4 and CD8 counts of that patient as determined by flow cytometry are depicted in Table The patients' HIV status, ... amounts to that obtained from infants via heelstick This mRNA was used to quantify the amount of CD4, CD8, as well as GAPDH, mRNA that is being transcribed in T lymphocytes within the blood The ... 5'CAAGTTGTCATGGATGACC3' Discussion Our results indicate that there is no correlation between CD4 and CD8 mRNA levels and numbers of cells expressing these proteins on their surface The formation of...
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Báo cáo y học: " Expansion of CD4+CD25+ helper T cells without regulatory function in smoking and COPD" potx
... to be inappropriate, which enables an uncontrolled progress of the immunoreaction, involving the activation of T cells into a cytotoxic phenotype This further supports a potential involvement ... on CD4+ cells is usually implied as regulatory T cells The present study shows that an increased percentage of of CD4+ CD25bright cells is associated to current smoking (Figure 2a) and that increased ... present data imply that tobacco smoking may induce an activation of airway CD4+ cells, in terms of increased CD127 expression and that the CD127 expression appears to decline after smoking cessation...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... vaccine is still not clear in this experimental setting A recent study has demonstrated that vaccination with DCs/tumor fusion cells producing TGF-β resulted in the induction of Treg in vivo and ... 3D) and to induce CTL responses against the HCC cells (Figure 3E), suggesting that the soluble factors in the supernatant inhibit the maturation of fusion cells and have a negative impact in the ... which Treg arise in vivo and exert their immunoregulatory effects remain to be defined and are the subject of intensive investigation In the present study, we first show that coculture of T cells...
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Báo cáo y học: "Indoleamine 2,3-dioxygenase-expressing dendritic cells are involved in the generation of CD4+CD25+ regulatory T cells in Peyer''''s patches in an orally tolerized, collagen-induced arthritis mouse model" ppt
... determining immunity or immune tolerance; this determination is based on the maturation or activation state and the subset of DCs, and cytokine profiles in the microenvironment at the time of antigen ... expression and inhibit T- cell proliferation In this regard, it will be interesting to investigate IDO expression in different subsets of DCs from tolerized mice and to characterize their role in the induction ... demonstrated that, after repeated oral administration of type II collagen (CII) and subsequent induction of CIA, the mean arthritis index is lower in tolerized mice than in CIA mice [12] and the...
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Báo cáo y học: "Decreased effector memory CD45RA+CD62L– CD8+ T cells and increased central memory CD45RA–CD62L+ CD8+ T cells in peripheral blood of rheumatoid arthritis patients" ppt
... patients are unknown Determination of these phenotypes in RA may provide important insights into T- cell homeostasis, and we therefore examined the distribution of CD4+ and CD8+ T cells into these ... effector memory CD8+ T cells in RA may indicate an increase in the migration of these cells into sites of inflammation, and therefore may contribute to ongoing synovial inflammation Competing interests ... et al tiation and migration into sites of inflammation An understanding of the mechanisms that are involved in this skewed differentiation of effector memory CD8+ T cells may prove valuable in...
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Báo cáo y học: "Expression of the inflammatory chemokines CCL5, CCL3 and CXCL10 in juvenile idiopathic arthritis, and demonstration of CCL5 production by an atypical subset of CD8+ T cells" pps
... T cells of this subpopulation Overall, this study contributes to our understanding of recruitment of T cells to the joint in inflammatory arthritis and suggests that in the microenvironment of ... the joint, dysregulation of functional patterns of expression may occur Competing interests The authors declare that they have no competing interests Authors' contributions DP and LRW generated ... of intracellular CCL5; positive staining for CCL5 was defined by comparison with isotype-matched control staining (c) The pattern of CCL5+ staining seen in CD8+ CD28+ T cells was seen in a total...
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The role of CD8 t cells in the differentiation of TNF iNOS producing dendritic cells and TH1 responses
... Abstract In this study, we showed that activated human CD8 T- cells could induce DCs to produce IL-12p70 in vitro and this interaction also resulted in the production of a cytokine milieu that ... supported by studies demonstrating the involvement of CD8 T cells in the generation of protective CD4 Th1 responses during retroviral infection (Peterson et al., 2002) Importantly, CD8 T cells ... up-regulated by factors that activate DCs including LPS and TNF-α Interestingly, recent studies have shown that memory CD8 T cells protected DCs from killing through cytotoxic granules by releasing TNF-α...
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Báo cáo y học: "Identification of clinical and simple laboratory variables predicting responsible gastrointestinal lesions in patients with iron deficiency anemia"
... 13.1% and gastrointestinal cancer in 11.2% of patients with IDA But two studies reported that IDA was one of the predictive factors of colorectal cancer and small intestinal cancer [19-20] The standard ... endoscopy in outpatients with iron deficiency anemia The aim of our study was to investigate the incidence of GI pathological findings in symptomatic and asymptomatic patients with IDA and to identify ... occult gastrointestinal bleeding Endoscopic evaluation of the gastrointestinal tract is commonly performed to evaluate iron deficiency Most of patients with iron deficiency in, whom gastrointestinal...
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Tài liệu Báo cáo khoa học: "Automatic Identification of Pro and Con Reasons in Online Reviews" ppt
... second last sentence in a paragraph These features test the intuition used in document summarization that important sentences that contain topics in a text have certain positional patterns in a paragraph ... labeling, Question Answering, and Information Extraction Maximum Entropy models implement the intuition that the best model is the one that is consistent with the set of constraints imposed by the evidence ... then trained our model using the training set and tested it to see if the system can successfully label sentences in the test set 4.2 Dataset 2: Complaints.com Data From the database in complaints.com,...
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Báo cáo khoa học: Tec family kinases: Itk signaling and the development of NKT ab and cd T cells potx
... cytokines, as well as alternative modes of development The data that are accumulating suggest that the role of Itk in the development and function of iNKT cells and cd T cells seems to be quite different ... levels of T- bet, and Itk may regulate the expression of this critical transcription factor Indeed, it has been suggested that Itk regulates T- bet levels in iNKT cells in the thymus, and that thymic ... the skin, intestinal epithelium and lung The different subsets of cd T cells express distinct cd TCRs and develop at different times in the thymus Skin cd T cells, also called skin-resident intraepithelial...
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Báo cáo khoa học: Identification of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathfinder selection pot
... Direct phage to intrabody screening: Demonstration by isolation of cytosolic intrabodies against the TES1 site of Epstein Barr virus latent membrane protein (LMP1) that block NF-jB transactivation ... be directly biotinylated in situ by the addition of biotinylated tyramine and easily recovered using streptavidin-coated beads Because the effective range of biotinylated tyramine is limited (a ... (a range of 15–20 nm, equivalent to 3–4 protein diameters), only PhAbs that bind in close proximity to the target antigen are selectively biotinylated It has been reported that these techniques...
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báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot
... present TAAgs, potency in stimulating T cell responses, stability of the phenotype following in vivo administration, the ability to migrate to sites of T cell activation and generation of CTLs Activated ... production of T cells that not recognize tumour cells in vivo based on this epitope [67-69] In contrast to these studies, the ability of our vaccination strategy to generate tetramer+ CD8+ T cells ... cycles of vaccination (the time point associated with the maximal tetramer + CD8+ response, in patients undergoing vaccination with class I peptides only),, in vitro cytotoxicity against both peptides...
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Báo cáo y học: "4th meeting of the EU research network EUROME: From the identification of genes and cellular networks in murine models of arthritis to novel therapeutic intervention strategies in rheumatoid arthritis, London, UK, 9 March 2004" pot
... cells in the TNF∆ARE model or stromal-radioresistant cells in the Tg197 hTNF+/– model) that suffice for the induction of arthritis In contrast, in all cases examined, the indispensable receptor ... alternative complement, its subsequent association with C5aR and FcgRIII-mediated cell activation resulting in innate cell mediator activation and the production of inflammatory cytokine interleukin-1 ... populations supporting the polarization of naive CD4+ T cells into Available online http://arthritis-research.com/content/6/4/155 either T helper (Th) or Th2 effector cells The goals of this arm of...
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Báo cáo y học: "Reduced proportions of natural killer T cells are present in the relatives of lupus patients and are associated with autoimmunity" ppt
... determined using a region that was set based on CD25 staining of the CD4population, so that under 1% of the CD4- population stained brightly, which permits identification of a population that is enriched ... Competing interests The authors declare that they have no competing interests Authors' contributions JW participated in study design; coordinated the flow cytometry, serologic studies, and data ... Society/CIHR Institute of Musculoskeletal Health and Arthritis and by The Arthritis Centre of Excellence of the University of Toronto Dr Hudson is the recipient of a Clinical-Scientist Award in Translational...
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