human embryonic stem cells in regenerative medicine

... Translational Medicine 2008, 6:54 cells, the number (cells/ mm2/mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected ... neuroprotection in the ischemic brain human embryonic stem cells diferentiationon OP9 feeder Day 10 Day VEGF-R2(+) / VE-cadherin(+) / TRA-1(-) cells VEGF-R2(+) / VE-cadherin (-) / TRA-1 (-) cells expansion ... human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of VEGF-R2, VE-cadherin and TRA-1 during the...

Ngày tải lên: 18/06/2014, 15:20

... human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are ... greater in hES cells than in EB cells and it was not even detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in ... upregulated in hES cells, node II contained the miRNAs upregulated in adult cells, node III contained the miRNAs upregulated in EB HMVEC = human microvascular endothelial cells, HUVEC = human umbilical...

Ngày tải lên: 18/06/2014, 15:20

... type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and sanctity of human life The President's ... approaches, in a manner to facilitate human ESC research and to promote respect for human embryos obtained from clinical reproductive endocrinology practice http://www.tbiomed.com/content/2/1/25 Human embryonic ... of additional human embryos Indeed, the embryos from which the existing ESCs originated have already been destroyed Federal funding of medical research on these existing stem cell lines will promote...

Ngày tải lên: 13/08/2014, 23:20

... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable ... importance of SHH in early mammalian development, there is significant value in studying its function in human embryonic stem cells (hESC), which are in vitro counterparts of the inner cell mass...

Ngày tải lên: 11/09/2015, 10:14

... staining (b) H1 von Kossa staining (c) hFOB H & E staining (d) hFOB von Kossa staining Figure At 4-week time point: H&E staining and von Kossa staining of sections showing scaffolds devoid of cells ... hFOB H & E staining (d) hFOB von Kossa staining Scaffold (e) hFOB H & E staining Figure At 2-week time point: H&E staining and von Kossa staining of sections showing scaffold and cells (e) hFOB ... time-point: (a) (c) nuclear staining with DAPI (b) Immunostaining with bone specific ALP (arrow showing the cells positive for ALP stain) (d) Immunostaining with osteonectin (arrow showing cells...

Ngày tải lên: 09/10/2015, 11:24

... is in need In this book, we explore the possibility of using human embryonic stem cells and derivatives as cellular model in implant testing for two main reasons One, human embryonic stem cells ... functions involving in embedding cells in the artificial implants Hence, regenerative medicine is always used synonymously with the term tissue engineering, although regenerative medicine emphases ... either from murine or human origin In this study, we investigated the efficiency of purmorphamine in differentiating human embryonic stem cell into osteoblast lineage by comparing with the traditional...

Ngày tải lên: 16/10/2015, 15:38

... of mitomycin C-inactivated murine embryonic fibroblast feeder (MEF) cells [7, 8] These were harvested from CF1 inbred mouse strain purchased from Charles River Laboratories Inc (Wilmington, MA, ... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure of human embryos and oocytes is a critical factor in determining the success of clinical assisted reproduction [15,...

Ngày tải lên: 31/10/2012, 16:57

... molecules on murine embryonic stem cells Biol Reprod 1997, 57(3):561-8 Bonde S, Zavazava N: Immunogenicity and engraftment of mouse embryonic stem cells in allogeneic recipients Stem Cells 2006, ... differentiation, blast cells have been resuspended in EGM-2 complete media (Cambrex) and incubated in fibronectin coated plates (Becton Dickinson) for days To prove that fibronectin-adhering cells are of ... genes In order to find the exact time point when blast colonyforming cells (BL-CFCs) arise in the EB system, we started a series of BL-CFC cultures on days to of EB differentiation in vitro In our...

Ngày tải lên: 18/06/2014, 15:20

... adult stem cells is their inability to effectively grow in Human embryonic stem cells and therapeutic cloning culture Therefore, obtaining clinically significant amounts of adult stem cells may ... transplantation medicine There are several approaches in human clinical trails that employ adult stem cells (such as blood-forming hematopoietic stem cells and cartilage-forming cells) A potential ... meiotic spindle after enucleation However, using a squeezing method, they are Human embryonic stem cells and therapeutic cloning successful in obtaining monkey cloned blastocysts [67], supporting our...

Ngày tải lên: 07/08/2014, 18:21

... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010, ... Rada-Iglesias A, Wysocka J: Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease Genome Medicine 2011, 3:36 ...

Ngày tải lên: 11/08/2014, 12:21

... of stem cells into the osteogenic lineage in vitro J Bone Miner Res 2004, 19:1379-1394 Page of 38 Trounson A: Human embryonic stem cells: mother of all cell and tissue types Reprod Biomed Online ... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... and in vivo mineralization of osteogenic cells derived from human embryonic stem cells Tissue Eng 2004, 10:1518-1525 17 Chaudhry GR, Yao D, Smith A, Hussain A: Osteogenic Cells Derived From Embryonic...

Ngày tải lên: 11/08/2014, 20:21

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... differentiation of human mesenchymal stem cells on laminin-5 J Cell Biochem (2):499-514 102 Lozito TP, Kuo CK, Taboas JM, et al 2009 ;Human mesenchymal stem cells express vascular cell phenotypes upon interaction ... colony-forming cell and the haemopoietic stem cell Blood Cells (12):7-25 32 Moore KA, Lemischka IR 2006 ;Stem cells and their niches Science (5769):1880-1885 33 Lemischka IR, Moore KA 2003 ;Stem cells: interactive...

Ngày tải lên: 11/09/2015, 09:16

... - GDF Haematoxylin and eosin - H&E Hours - hr Human- specific vimentin - hVIM Human embryonic stem cells - hESCs Human ESC-derived chondrogenic cell-engineered cartilage - HCCEC Human palate mesenchymal ... emerging trends in cartilage tissue engineering and regenerative medicine In particular, we will be focusing on ESCs as a promising cell source for cartilage regeneration, the recent advances in ... Arthritis Foundation] Challenges faced in cartilage tissue engineering and regenerative medicine are attributable in large part to the intrinsic biology of cartilaginous tissue, which limits its capacity...

Ngày tải lên: 11/09/2015, 09:57

... classified into six broad groups according to their derivation sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem ... linked to proteins in the form of proteoglycans The other is fibrous proteins, including collagen, fibronectin and laminin, etc Proteoglycan is thought to have a major role in chemical signaling ... culture and maintenance of hESCs on human laminin in culture medium supplemented with either activin A (Beattie et al., 2005) or a cocktail of growth factors, including human FGF-2, human stem cell...

Ngày tải lên: 11/09/2015, 10:00

... fluorescent protein ES cells FBS hES cells embryonic stem cells fetal bovine serum human embryonic stem cells HIV human immunodeficiency virus HSV herpes simplex virus ITR inverted terminal repeat ... choosing various promoters and viral modulators to achieve desirable level of transient transgene expression in human embryonic stem cells Efficient gene transfers to human embryonic stem cells ... rejection in transplantation medicine, and providing cell sources with new functional properties to combat specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human...

Ngày tải lên: 11/09/2015, 14:38

... (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain stem cells ... experiments involving blastocyst injections and ectopic grafting in adult hosts cannot be performed in the human Animal embryonic stem cells Embryonic stem cells were first derived from certain strains ... categories of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their...

Ngày tải lên: 16/09/2015, 15:55

... CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes for effective ... Chapter 1: General Introduction 1.1 Embryonic stem cells 1.2 Gastrulation– formation of mesoderm and endoderm in the embryo .8 1.3 Regenerative medicine and embryonic stem cells 10 ... Biology 1.3 Regenerative medicine and embryonic stem cells 1.3.1 Diabetes– a candidate disease for cell therapy Autoimmune destruction of insulin-secreting pancreatic β -cells within the Islets...

Ngày tải lên: 04/10/2015, 16:03

... WNT wingless-type MMTV integration site family Page X Chapter Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells ... cells LIN28, an RNA binding protein, contains three RNA binding domains: one coldshock domain at the N terminus and two CCHC-type zinc finger domains at the C terminus It was originally found to regulate ... IX Introduction 1.1 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced...

Ngày tải lên: 16/10/2015, 11:58

... RESULTS……………………………………………………… 27 4.1 Characterization of human embryonic stem cells …………………… 27 4.2 Temperature tolerance of human embryonic stem cells …………… 30 4.21 Survival rate of hESC after exposure ... polymerase Chain Reaction (rtPCR) 29 Figure hESCs maintained at 37oC (physiological control) by PNA FISH 33 Figure Figure hESCs maintained at oC for 24h by PNA FISH hESCs maintained at 25 oC ... However, established cell lines poorly reflect human physiology Lack of standardization is also a limitation with the use of primary explanted cells Human embryonic stem cells (hESCs) have been...

Ngày tải lên: 22/10/2015, 21:13