an alternate source of pluripotent stem cells for regenerative medicine

... and induced pluripotent stem cells Cell Stem Cell 2010, 7:249-257 Wilson KD, Venkatasubrahmanyam S, Jia F, Sun N, Butte AJ, Wu JC: MicroRNA profiling of human-induced pluripotent stem cells Stem ... Feinberg AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 ... of induced pluripotent stem cells for regenerative medicine Nat Cell Biol 2011, 13:497-505 Zhu H, Lensch MW, Cahan P, Daley GQ: Investigating monogenic and complex diseases with pluripotent stem...

Ngày tải lên: 11/08/2014, 12:21

... degenerated cells and the transplanted stem cells 4.2 Stem cell transplantation Stem cells are defined by their capability for self-renewal and multipotent differentiation On the basis of these ... Ang, ex-Head of Anatomy Department, and Professor Bay Boon Huat, current Head of Anatomy Department, National University of Singapore, for their constant support and encouragement, and for their ... current understanding of etiology and pathogenesis of INTRODUCTION PD, the application of animal models for studying PD, and possible therapeutic application of stem cells in the treatment of PD will...

Ngày tải lên: 11/09/2015, 09:05

... methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 101 Santenard A, ... [91,95] Relevance of epigenomics for human disease and regenerative medicine In this section, we envision how recent advances in epigenomics can be used to gain insight into human development and disease, ... Enhancer Enhancer (c) Enhancer Gene Enhancer Gene Gene Gene Enhancer Gene Active promoter Developmental gene Poised enhancer Gene Enhancer (b) Gene Gene Enhancer Active enhancer Enhancer Enhancer...

Ngày tải lên: 11/08/2014, 12:21

... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... analysis, Petra Kraus and Han Jianyong for relevant mouse work, Lim Seong Soo for karyotyping, and Ng Jia Hui for help with the microarray analysis I also thank Kyle M Loh for his wonderful intelligent ... reprogramming (formerly thought indispensible), and sheds light on the mechanism of action of Nr5a2 ix List of Tables Table List of 24 transcription factors screened by Takahashi and Yamanaka for reprogramming……………………………………………………………………….27...

Ngày tải lên: 09/09/2015, 18:56

... 2.6 Manufacturer Registered office New England Biolabs Frankfurt, Germany New England Biolabs Frankfurt, Germany New England Biolabs Frankfurt, Germany New England Biolabs Frankfurt, Germany New ... suitable candidates for transduction 1.3.3 Alternative systems for genetic modifications of human cells Besides the already listed viral and electroporation systems, other methods for genetic manipulations, ... pluripotent stem cells Landmark discoveries of the young field of human stem cell science were the isolation and culture of inner cell mass from human blastocysts by Bongso in 1994 and in the...

Ngày tải lên: 26/11/2015, 09:54

... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The ... exploration and realization of the incredible potential of these pluripotent stem cells for studying the fundamentals of early human development and organogenesis, for cellbased therapy and for disease ... long-term culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube...

Ngày tải lên: 22/03/2014, 09:20

... needed Another important issue concerning the therapeutic use of cells as vehicles is the quantity of cells reaching the desired destination, as well as an understanding of how these numbers translate ... (211744 ± 178135 and 91023 ± 84675 cells/ mg protein), and corresponded only to 2.3 times difference The means and the standard deviation range was affected by the small number of animals and individual ... biodistribution and homing of injected cells, we reasoned that an effect of engineered cell targeting would be more pronounced and better detectable in model systems For instance, an isolated heart...

Ngày tải lên: 20/06/2014, 07:20

... Gaussian with zero mean and variance s2, and the SNR is defined as L 10log10 pn /(Lσ ) n=1 The angle scanning grid is uniform in the range from 1° to 180° with 1° increment between adjacent angle ... http://asp.eurasipjournals.com/content/2012/1/7 Page of Figure Performance comparison of IAA and EIAA (a-c) For SNR = dB; (d-f) for SNR = 10 dB (a, d) Angle error difference power power angle angle ; (b, e) power error ... of EIAA and IAA er − er EIAA IAA EIAA has slightly better angle and power estimation accuracy than IAA As for the computational efficiency, the running time of EIAA is less than 15% of that of...

Ngày tải lên: 20/06/2014, 20:20

... cell source are adult stem cells, in particular, bone-marrow-derived mesenchymal stem cells (MSCs) The use of these cells would allow an autologous approach, reducing the risk of rejection and ... field of view was performed to enable identification of GFP-positive cells and immunopositivity for matrix proteins in the same cells Results Identification of injected mesenchymal stem cells ... Omi H, Watanabe T, Serigano K, Tamura F, Sakai D: Transplantation of mesenchymal stem cells in a canine disc degeneration model J Orthop Res 2008, 26:589-600 Page of 10 (page number not for citation...

Ngày tải lên: 09/08/2014, 01:22

... differentiation, and isotype-switching of B cells/ B cells Ligand for CD40/T cells Critical for T and B cell antigen receptor mediated activation/ all haematopoietic cells Ligand for CD28 and CD152 (CTLA-4), ... costimulation/B cells Ligand for CD28 and CD152 (CTLA-4)/B cells Growth factor receptor/haemopoietic stem and progenitor cells, mast cells Function unknown/haematopoietic stem cells and precursors ... the auspices of the European Group for Blood and Marrow Transplantation stem cell subcommittee targeting acute GvHD treatment and prevention, using standardized expansion protocols and defined...

Ngày tải lên: 09/08/2014, 08:23

... far been the only source for human ESCs The fact that live human embryos must be destroyed to produce human ESCs presents a substantial ethical obstacle for the advancement of human ESC research ... blastocysts and re-plated on to fresh feeder cells for confirmation and further analysis; no cells were frozen This work carries forward a theoretical approach suggested more than a decade ago [12], and ... belief in the fundamental value and sanctity of human life The President's decision reflects a commitment to preserve the value and sanctity of human life balanced with a desire to promote vital...

Ngày tải lên: 13/08/2014, 23:20

... period of time (usually weeks for mouse cells, and 3-4 weeks for human cells) Whether reprogramming by defined factors can be further optimized will depend on a better understanding of the mechanisms ... and genetic approach for the generation of induced pluripotent stem cells Cell Stem Cell 2008, 2:525-528 Shi Y, Desponts C, Do JT, Hahm HS, Schöler HR, Ding S: Induction of pluripotent stem cells ... transposition of PiggyBac in mouse embryonic stem cells Proc Natl Acad Sci USA 2008, 105:9290-9295 Ding S, Wu X, Li G, Han M, Zhuang Y, Xu T: Efficient transposition of the piggyBac (PB) transposon...

Ngày tải lên: 14/08/2014, 21:20

... mean ± standard error (SEM) Comparisons between two groups were made with the use of analysis of variance (ANOVA) and Newman-Keuls post-hoc analysis A p value of < 0.05 was considered significant ... Gallagher, Dr Johnathan Tune, and Dr Michael Sturek They cheer for my progress and caution me of pitfalls in front They are my guardian angels I also thank American Heart Association and Cryptic Masons ... life with plenty of joy, happiness, and thankfulness   v ABSTRACT Jie Xie TSG-6: AN INDUCIBLE MEDIATOR OF PARACRINE ANTI-INFLAMMATORY AND MYELOPROTECTIVE EFFECTS OF ADIPOSE STEM CELLS Tumor necrosis...

Ngày tải lên: 24/08/2014, 11:56

... temporary relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) ... expansion of the differentiated cells, phenotypic stability of differentiated cells and tissues, delivery systems and biomaterials for cartilage repair, and survivability and integration of the ... sincere thanks to Assistant Professor Jerry Chan, Experimental Fetal Medicine Group, Yong Loo Lin School of Medicine, National University of Singapore, for his advice on human chimerism studies and...

Ngày tải lên: 11/09/2015, 09:57

... Ling Qian, Eugene Sim, Philip Wong and Winston Shim Myocardial Matrix-driven Cardiac Differentiation and Integration of Human Mesenchymal Stem Cells [Manuscript submitted to Stem Cells and Development] ... author for the abstract, entitled, “Large Scale Expansion of Human Cardiomyocyte-Like Cells for Cell Therapy” and of poster finalists in the Basic Science category of Stem/ Progenitor Cells in ... coronary intervention for STEMI Functional recovery of cardiac function following transplantation of BM included a significant reduction in infarct size and an enhanced recovery of regional systolic...

Ngày tải lên: 12/09/2015, 09:42

... undifferentiated, quiescent state For stem cells to be activated, the niche environment must change (Fuchs, 2007) 1.4.3 Stem cells and cancer Stem cells and cancer cells share many common properties including ... transformation of normal stem cells Because of their extended lifespan and self-renewal properties stem cells are easier to transform compared to differentiated cells through the accumulation of ... enhancer of split Human embryonic stem cells Heparan sulfate Haematopoietic stem cell Heparan sulfate proteoglycan Inner cell mass Iduronic acid IGF binding protein Inducible pluripotent stem cells...

Ngày tải lên: 12/09/2015, 21:26

... abilities of stemcells Hence, NANOG is essential for the maintenance of human Embryonic Stem Cells in vitro The mRNA of NANOG is only present in undifferentiated and pluripotent mouse and human cell ... Induced Pluripotent Stem Cells Embryonic stem cells (ESCs) hold great promises for regenerative medicine as they are pluripotent and are able to grow indefinitely (Evans & Kaufman, 1981) However, many ... addition, human Embryonic Stem Cells can be used as a platform for drug discovery and toxicology as they can be used as biotools for the discovery of drugs that may activate the cells for regeneration...

Ngày tải lên: 30/09/2015, 10:13

... feeder cells for weeks before addition of 1uM of CDy9 and image acquisition Cells from dim population were seeded onto mitomycin treated feeder cells for weeks before addition of 1uM of CDy9 and ... mixtures of cells and 48 individual dimly stained mixtures of cells was determined by an array of primers from the TaqMan® Gene Expression Assay A total of 33 primers were selected to determine the pluripotent ... upregualtion of pluripotent genes for mESC and an upregulation of differentiated genes for the MEF cells (b) The PCA plot shows that the populations of cells are clearly separated from one another The...

Ngày tải lên: 02/10/2015, 17:14

... a readily available source of pluripotent human stem cells which can be utilized for the treatment of many diseases Figure 12: A general workflow of target identification for CDy9 The compounds ... Induced Pluripotent Stem Cells 2008 Cell Stem Cell 595-605 10) M F Pera, B Reubinoff and A Trounson Human embryonic stem cells 2000 Journal of Cell Science.113, 5-10 11) J A Thomson Embryonic Stem ... Robbins, and J C Wu, Single cell transcriptional profiling reveals heterogeneity of human induced pluripotent stem cells 2011 J Clin Invest 121(3):1217–1221 33) S W Yun, N Y Kang, and Y T Chang Diversity...

Ngày tải lên: 02/10/2015, 17:14