hpiec of mono and oligosaccharides

Basic Methods for the Biochemical Lab  Martin Holtzhauer

Basic Methods for the Biochemical Lab Martin Holtzhauer

... 50 vol of Soln A with 48 vol of Soln B and vol of Soln C The standard curve is made between and 100 µg BSA/100 µl Fill up 100 µl of sample or standard to 100 µl with ddH2 O, if necessary, and mix ... to 0.10 ml of sample, and then add 1.0 ml Soln E, and thereafter add 0.1 ml Soln D Measure after 30–45 Prepare the standard curve in the range between and 30 µg of protein Since the standard curve ... mix 100 vol of Soln A with vol of Soln B Stable at least for week at RT Standard: 1.0 mg/ml BSA4 Solutions/Reagents The standard curve is made between and 100 µg Fill samples and standards up...

Ngày tải lên: 20/08/2016, 14:35

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Credit guidelines for HE qualifications in England, Wales and Northern Ireland docx

Credit guidelines for HE qualifications in England, Wales and Northern Ireland docx

... institutions in England, Wales and Northern Ireland, and to • provide information on the relative credit values of the principal HE qualifications The status of these guidelines The content of this document ... for England, Wales and Northern Ireland [EWNI] and a separate framework for Scotland The purpose of the frameworks is to bring consistency to the nomenclature used for HE qualifications and to ... significant and original contribution to a specialised field of enquiry demonstrating a command of methodological issues and engaging in critical dialogue with peers Display mastery of a complex and...

Ngày tải lên: 29/03/2014, 03:21

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Florence and Northern Tuscany with Genoa potx

Florence and Northern Tuscany with Genoa potx

... VII of Germany, St Catherine of Siena, and often too, St Catherine Adorni, Louis XII of France, Don John of Austria after Lepanto, and maybe, who knows, Velasquez of Spain, Vandyck from England, ... slain, and Count Ugolino was taken and two of his sons and three grandsons, his son's children, and they were put in prison; and his household and followers, the Visconti and Ubizinghi, Guatini and ... There was taken Andrea Morosini of Venice, then Podestà and Captain General in war of the Commune of Pisa, with the standard of the Commune, captured by the galleys of Doria and brought to this...

Ngày tải lên: 31/03/2014, 13:20

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báo cáo sinh học:" Non-European Union doctors in the National Health Service: why, when and how do they come to the United Kingdom of Great Britain and Northern Ireland?" pdf

báo cáo sinh học:" Non-European Union doctors in the National Health Service: why, when and how do they come to the United Kingdom of Great Britain and Northern Ireland?" pdf

... Percentage of responses Year of primary medical qualification Figure Diagram1showing the year of primary qualification of overseas doctors Diagram showing the year of primary qualification of overseas ... (18.9%), preference of living in the UK (7.4%) and the presence of family and friends in the UK (7.1%) (Figure 4) All respondents were asked to report their 'main' and 'other' reasons of immigration ... the career paths of many non-European doctors, many of whom have spent a considerable duration of time working and training in the National Health Service of United Kingdom Page of (page number...

Ngày tải lên: 18/06/2014, 17:20

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Indian and Northern Affairs Canada Internal Audit Report Financial Audit of the Settlement Allotment Prepared by: Audit and Assurance Services Branch April 9, 2009 pptx

Indian and Northern Affairs Canada Internal Audit Report Financial Audit of the Settlement Allotment Prepared by: Audit and Assurance Services Branch April 9, 2009 pptx

... Allotment funds and compliance with the terms of the TB decision and applicable INAC and TB policies The scope of the audit included an overall assessment and detailed testing of a sample of transactions ... the requirements of the TB Policy on Internal Audit and followed the Institute of Internal Auditors’ Standards for the Professional Practice of Internal Auditing Sufficient and appropriate audit ... within the Resolution and Individual Affairs and Chief Financial Officer Sectors and identification and assessment of the existing management control framework for the payment of funds against the...

Ngày tải lên: 20/06/2014, 06:20

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Air Quality Pollutant Inventories for England, Scotland, Wales and Northern Ireland: 1990 – 2010 doc

Air Quality Pollutant Inventories for England, Scotland, Wales and Northern Ireland: 1990 – 2010 doc

... developed in the late 1990s (in England, Wales and Northern Ireland) and early 2000s (in Scotland); • Emissions from oil and gas terminals and offshore platforms and rigs, are based on operator estimates ... developed in the late 1990s (in England, Wales and Northern Ireland) and early 2000s (in Scotland); • Emissions from oil and gas terminals and offshore platforms and rigs, are based on operator estimates ... England, Scotland Wales and Northern Ireland: 1990-2010 Air Quality Pollutants Air Quality Pollutants Inventories for England, Scotland, Wales and Northern Ireland for ammonia (NH3), carbon monoxide...

Ngày tải lên: 28/06/2014, 21:20

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The Air Quality Strategy for England, Scotland, Wales and Northern Ireland Volume I pdf

The Air Quality Strategy for England, Scotland, Wales and Northern Ireland Volume I pdf

... loss of yield and quality of crops, damage to forests and impacts on biodiversity 17 The Air Quality Strategy for England, Scotland, Wales and Northern Ireland (Volume 1) Pollutant Description and ... England, Scotland, Wales and Northern Ireland – Working Together for Clean Air, January 2000 (Cm 4548, SE2000/3, NIA 7) 4  The Air Quality Strategy for England, Scotland, Wales and Northern Ireland: ... Quality Strategy for England, Scotland, Wales and Northern Ireland (Volume 1) Presented to Parliament by the Secretary of State for Environment, Food and Rural Affairs By Command of Her Majesty: Laid...

Ngày tải lên: 28/06/2014, 21:20

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Báo cáo khoa học: "Cattle brucellosis in traditional livestock husbandry practice in Southern and Eastern Ethiopia, and its zoonotic implication" ppsx

Báo cáo khoa học: "Cattle brucellosis in traditional livestock husbandry practice in Southern and Eastern Ethiopia, and its zoonotic implication" ppsx

... classifications of lowland “Kola” (< 1500 m.a.s.l.); midland “Weynadega” (1500 2400 m.a.s.l.) and highland “Dega” (> 2400 m.a.s.l.) Geographically, the study areas cover latitude and longitude ranges of 03° ... the research and extension office of Hawassa University and the National Animal Health Diagnostic and Investigation Center (NAHDIC), in addition to the willingness and cooperation of animal owners ... based on geographic localities and dominant livestock production system, whereas districts and villages were randomly selected following a randomization of districts and villages when lists were...

Ngày tải lên: 12/08/2014, 18:22

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Báo cáo khoa học: "Case mix, outcome and length of stay for admissions to adult, general critical care units in England, Wales and Northern Ireland: the Intensive" pptx

Báo cáo khoa học: "Case mix, outcome and length of stay for admissions to adult, general critical care units in England, Wales and Northern Ireland: the Intensive" pptx

... of patient gender [40] and socioeconomic status (Hutchings A, personal communication, 2002), of day and time of admission to critical care [41], of time of discharge from critical care [42] and ... R102 all data (of the hospital, of the unit, of the staff and of the patient) shall remain confidential Demographics Individual admissions are identified by an admission number and an alphanumeric ... critical care unit and from hospital are also recorded Activity The length of stay in the CMP unit is calculated (in fractions of days) from the dates and times of admission to and discharge from...

Ngày tải lên: 12/08/2014, 20:20

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Báo cáo khoa học: "The epidemiology of severe sepsis in England, Wales and Northern Ireland, 1996 to 2004: secondary analysis of a high quality clinical" pps

Báo cáo khoa học: "The epidemiology of severe sepsis in England, Wales and Northern Ireland, 1996 to 2004: secondary analysis of a high quality clinical" pps

... Netherlands [26], 77/100,000 in Australia and New Zealand [27], and 75 to 119/100,000 in the USA [21]) The difference may, therefore, reflect a lower provision of critical care beds in England, ... analysis, and drafted the manuscript CW participated in the analysis JE conceived the study All authors were involved in interpretation of data and critical revision of the manuscript, and have read and ... total numbers of admissions aged 16 years or over to adult, general critical care units in England, Wales and Northern Ireland for each year were estimated by extrapolating the number of observed...

Ngày tải lên: 12/08/2014, 23:22

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Thiết lập qui trình southern blot

Thiết lập qui trình southern blot

... miếng gel Bật UV, định vị band cần lấy, dùng dao lam cắt gel chứa band DNA cần tinh sạch, cho vào eppendorf 1,5 ml Sử dụng cân phân tích để xác định trọng lượng gel chứa band vừa cắt Dùng micropipette ... primer thiết kế gen phlD Hình 3.2 Tổ hợp gen sinh tổng hợp chất 2,4 – DAPG Trong gen phlD tổng hợp monoacetylphloroglucinol (MAPG) gen phlA, phlC, phlB cần thiết cho trình chuyển đổi MAPG thành DAPG ... 30 giây Cho thêm vào cột 500 µl wash buffer, ly tâm 10000 vòng / 30 giây Lấy cột đặt vào eppendoft 1,5 ml Hút 20 µl elution buffer nhỏ vào cột GFX, ủ nhiệt độ phòng phút 10 Ly tâm 10000 vòng...

Ngày tải lên: 01/11/2012, 10:59

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 Thiết lập qui trình southern  blot 3

Thiết lập qui trình southern blot 3

... lần; lần; lần Mẫu 130 sau pha loãng lần có độ sáng band sau điện di tương đồng với band 200 bp phân tử Mass (hình 4.3) Theo chuẩn phân tử Mass, band 200 bp có nồng độ tương đương 20 (ng / µl) Vậy ... toàn trước hòa tan với TE - Eppendorf chứa mẫu DNA phải - Hóa chất ly trích như: phenol / chloroform / isoamyl alcohol; CTAB có khả gây độc cho người sử dụng, sử dụng pha chế phải thực buồng ... cho phản ứng cắt hoàn toàn so với mẫu DNA tổng số chưa cắt (giếng hình 4.4a), mẫu 130 cắt phần, band genomic DNA đậm Đối với hình 4.4b, mẫu 123 124 cắt với ba enzyme BamH I, Eco RV Hind III, hai...

Ngày tải lên: 01/11/2012, 10:59

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 Thiết lập qui trình southern blot 1

Thiết lập qui trình southern blot 1

... dung dịch DNA với phenol / chloroform / isoamyl alcohol (25: 24: 1) Hòa lẫn đều, ly tâm 12000 vòng/5 - 10 phút/4oC 13 Thu phần dung dịch bên chiết xuất với chloroform / isoamyl alcohol (24:1) ... / chloroform, lắc có hòa lẫn hoàn toàn Cẩn thận chuyển hỗn dịch DNA phenol vào tube Phase Lock Gel TM ly tâm phút Lấy phần dịch bên cho vào eppendorf thêm vào lượng ngang phenol / chloroform Trộn ... bromide xem UV 11 2.6.3 Định lƣợng DNA phân tử Mass Phương pháp dựa vào độ sáng band cần định lượng so với band phân tử Mass Mẫu định lượng cần phải pha loãng lần, hai lần, ba lần, …, n lần...

Ngày tải lên: 01/11/2012, 10:59

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Bước đầu ứng dụng kỹ thuật southern Blot phân tích đa dạng  di truyền nấm

Bước đầu ứng dụng kỹ thuật southern Blot phân tích đa dạng di truyền nấm

... Analysis and PCR-based Diagnosis of the Population of Magnaporther grisea The Graduate School of Science and Technology Kobe University, pp 31-37 12 Le Dinh Don,Yukio Tosa, Hitoshi Nakayashiki and ... conservation of a family of repeat DNA sequences in the genome of a fungal plant pathogen Proc Nat Acad Sci USA 86: 9981-9985) 10 Jose Romao and John E Hamer, 1992 Genetic organization of repeated ... varios Hosts Revealed by Polymorphisms of Nuclear Ribosomal DNA and the Distribution of the MAGGY Retrostranposon Annals of the Phytopathological Society of Japan ...

Ngày tải lên: 05/11/2012, 13:58

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Ứng dụng kỹ thuật southern blot phân tích đa dạng di truyền nấm Magnaphorthe grisea gây bệnh đạo ôn trên cây lúa

Ứng dụng kỹ thuật southern blot phân tích đa dạng di truyền nấm Magnaphorthe grisea gây bệnh đạo ôn trên cây lúa

... Analysis and PCR-based Diagnosis of the Population of Magnaporther grisea The Graduate School of Science and Technology Kobe University, pp 31-37 12 Le Dinh Don,Yukio Tosa, Hitoshi Nakayashiki and ... conservation of a family of repeat DNA sequences in the genome of a fungal plant pathogen Proc Nat Acad Sci USA 86: 9981-9985) 10 Jose Romao and John E Hamer, 1992 Genetic organization of repeated ... varios Hosts Revealed by Polymorphisms of Nuclear Ribosomal DNA and the Distribution of the MAGGY Retrostranposon Annals of the Phytopathological Society of Japan ...

Ngày tải lên: 19/11/2012, 15:13

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KHÓA LUẬN TỐT NGHIỆP: THIẾT LẬP QUI TRÌNH SOUTHERN BLOT (tài liệu tham khảo)

KHÓA LUẬN TỐT NGHIỆP: THIẾT LẬP QUI TRÌNH SOUTHERN BLOT (tài liệu tham khảo)

... Raaijmakers, Linda S Thomashow, and David M Weller, 2000 Genotypic and Phenotypic Diversity of phlD - Containing Pseudomonas Strains Isolated from the Rhizosphere of Wheat Applied And Environmential Microbiology, ... 76 Differential Ability of Genotypes of 2,4 Diacetylphloroglucinol Producing Pseudomonas fluorescens Strains To Colonize the Roots of pae Plants Applied And Environmential Microbiology, ... London Tokyo 14 Boulnois J G., 1987 Gene Cloning and Analysis A Laboratory Guide Compiled by the Staff of the University of Leicester Gene Cloning Course and edited by Blackwell Scientific Publications,...

Ngày tải lên: 19/03/2013, 09:33

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