harvesting purification and titration of hsv 1 amplicon vectors

Báo cáo y học: "Egress of HSV-1 capsid requires the interaction of VP26 and a cellular tetraspanin membrane protein" doc

... production of Wang et al Virology Journal 2 010 , 7 :15 6 http://www.virologyj.com/content/7 /1/ 156 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 infectious virus in the nervous system of infected ... mutant HSV1 -UG were observed under fluorescence microscope at 12 , 16 and 24 h b Growth curve of HSV1 -UG compared with that of wild type HSV- 1 in Vero cells c Electro-microscope observation of cells ... visualization of Page 12 of 12 herpes simplex virus type compartment dynamics J Virol 2008, 82:4974-4990 doi: 10 .11 86 /17 43-422X-7 -15 6 Cite this article as: Wang et al., Egress of HSV- 1 capsid requires...

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Báo cáo khoa học:" A role for the JAK-STAT1 pathway in blocking replication of HSV-1 in dendritic cells and macrophages" docx

Danh mục: Cao đẳng - Đại học

... #) 7.0 10 STAT1-/STAT1+/+ 6.0 10 5.0 10 4.0 10 3.0 10 2.0 10 1. 0 10 12 24 48 Hours Post Infection C 2.3 10 gB DNA/Ml (Copy #) 2.0 10 1. 8 10 1. 5 10 1. 3 10 1. 0 10 7.5 10 5.0 10 2.5 10 0 12 24 48 ... http://www.virologyj.com/content/6 /1/ 56 STAT1 -/STAT1 +/+ ICP4 ICP0 Normalized Fold Expression Normalized Fold Expression 6.0 10 1. 2 10 1. 0 10 8.0 10 6.0 10 4.0 10 2.0 10 12 24 5.0 10 4.0 10 3.0 10 2.0 10 1. 0 10 48 Hours ... http://www.virologyj.com/content/6 /1/ 56 BALB/c (1 PFU/Cell) B LB/c (10 PFU/Cell) STAT1 +/+ (10 PFU/Cell) STAT1 -/- (10 PFU/Cell) A 10 Virus Titer (PFU/Ml) 10 10 10 10 10 10 10 10 10 12 24 48 Hours Post Infection...

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Báo cáo khoa học: " A role for the JAK-STAT1 pathway in blocking replication of HSV-1 in dendritic cells and macrophages" pps

Danh mục: Báo cáo khoa học

... #) 7.0 10 STAT1-/STAT1+/+ 6.0 10 5.0 10 4.0 10 3.0 10 2.0 10 1. 0 10 12 24 48 Hours Post Infection C 2.3 10 gB DNA/Ml (Copy #) 2.0 10 1. 8 10 1. 5 10 1. 3 10 1. 0 10 7.5 10 5.0 10 2.5 10 0 12 24 48 ... http://www.virologyj.com/content/6 /1/ 56 STAT1 -/STAT1 +/+ ICP4 ICP0 Normalized Fold Expression Normalized Fold Expression 6.0 10 1. 2 10 1. 0 10 8.0 10 6.0 10 4.0 10 2.0 10 12 24 5.0 10 4.0 10 3.0 10 2.0 10 1. 0 10 48 Hours ... http://www.virologyj.com/content/6 /1/ 56 BALB/c (1 PFU/Cell) B LB/c (10 PFU/Cell) STAT1 +/+ (10 PFU/Cell) STAT1 -/- (10 PFU/Cell) A 10 Virus Titer (PFU/Ml) 10 10 10 10 10 10 10 10 10 12 24 48 Hours Post Infection...

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Purification and cloning of PCR products

Danh mục: Môi trường

... FS (19 91) Construction of T -vectors, a rapid and general system for direct cloning of unmodiﬁed PCR products Nucleic Acids Res 19 : 11 54 Pan G, Luetke K, Juby CD, Brousseau R, Sadowski P (19 93) ... requirement of the single-stranded tails for ligation-independent cloning (LIC) of PCR products PCR Methods Appl 4: 17 2 17 7 Puriﬁcation and cloning of PCR products 13 3 14 Haun RS, Moss J (19 92) Ligation-independent ... at one side of a piece of preboiled (1) dialysis tubing that also contains a small Puriﬁcation and cloning of PCR products 11 5 volume (10 0–500 µl depending on the gel slice size) of the agarose...

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Báo cáo khoa học: Adaptability and ﬂexibility of HIV-1 protease potx

Danh mục: Báo cáo khoa học

... superposition of unliganded C95M/C1095A structure with each of the six cyclic urea-complexed structures shown in Fig 2B 1LV1 1LV1 1HWR 1DMP 1QBS 1HVR 1QBR 1QBT 1HWR 1DMP 1QBS 1HVR 1QBR 1QBT 0.00 ... 1HVR, 1QBS, 1DMP, 1QBR and 1QBT) are of complexes between different types of inhibitor molecules and HIV -1 protease containing the C95A mutation In two of these structures (1DAZ and 1A94), the ... of the bound ligand Fig shows the superposition of peptide complex structure 1DAZ with unliganded structure 1LV1 Interestingly, the catalytic aspartates Asp25 /10 25 in the two structures (1LV1...

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báo cáo hóa học:" The HIV-1 Non-subtype B Workgroup: An International Collaboration for the Collection and Analysis of HIV-1 Non-subtype B Data" pot

Danh mục: Hóa học - Dầu khí

... purposes) Journal of the International AIDS Society 2005, 7: 71 10 11 12 13 14 15 http://www.jiasociety.org/content/7 /1/ 71 Kantor R, Katzenstein D, Camacho R, et al.: Genotypic analyses of RT and protease ... analysis of RT and protease sequences and data from persons infected with non-B HIV -1 subtypes Currently, the workgroup consists of investigators from 15 sites in 13 countries The goal of the workgroup ... sequences Program and abstracts of the 11 th Conference on Retroviruses and Opportunistic Infections; February 811 , 2004; San Francisco, California Abstract 59 Publish with Bio Med Central and every...

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Báo cáo hóa học: " Effective harvesting, detection, and conversion of IR radiation due to quantum dots with built-in charge" docx

Danh mục: Hóa học - Dầu khí

... converters Proc IRE 19 60, 48 :12 46 Sablon et al Nanoscale Research Letters 2 011 , 6:584 http://www.nanoscalereslett.com/content/6 /1/ 584 10 11 12 13 14 15 16 17 18 19 20 21 Page 13 of 13 Shockley W, ... and B52) and with inter-dot doping (devices B45 and B53) In devices B44 and B52 (Figure 1a), the dopant sheet concentration was 2.7 × 10 11 cm-2 and 5.4 × 10 11 cm-2, respectively Devices B45 and ... Research Letters 2 011 , 6:584 http://www.nanoscalereslett.com/content/6 /1/ 584 Page 10 of 13 Figure Comparison of PL spectral dependences Comparison of PL spectral dependences of n- and p-doped samples...

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Báo cáo hóa học: " Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus" pot

Danh mục: Hóa học - Dầu khí

... 0 .1 0. 01 0 10 0 76 98 81 100 93 0 73 FI 602 1. 8 0 EDTA 10 0 10 0 10 0 10 0 10 0 10 0 FII 44 0 Chymostatin 0 .1 19 10 0 76 10 0 19 90 FIII -1 393 0 .1 Pepstatin 10 0 58 60 86 83 75 FIII-2 783 0.4 FIII-3 8 31 ... RB (19 87) Fibrinogen and risk of cardiovascular disease The Framingham Study JAMA 258(9) :11 83 11 86 doi :10 .10 01/ jama.258.9 .11 83 Lowry OH, Rosenberg WJ, Farr AL, Randall RJ (19 51) Quantization of ... Jpn J Physiol 41( 3):4 61 72 doi :10 . 217 0/jjphysiol. 41. 4 61 Mosesson MW (2005) Fibrinogen and fibrin structure and functions J Thromb Haemost 3(8) :18 94–904 doi :10 .11 11/ j .15 38-7836.2005. 013 65.x Mosesson...

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Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc

Danh mục: Báo cáo khoa học

... noitacifiruP .38-57 , 21 ,09 91 lonummI etisaraP sulporcim sulihpooB htiw noitatsefni tsniaga elttac drofereH tcetorp snegitna enarbmem lavraL PJ kceebedpO ,MYJ gnoW 03 15 31- 64 31 ,3 41 ,98 91 lonummI J sulporcim ... ,AG gnidiR ,P nesdalliW 92 8 91- 6 91 ,4 ,88 91 yadoT lotisaraP lortnoc kcit rof snegitna 'delaecnoc' htiw noitaniccaV HD pmeK ,P nesdalliW 82 05-34S ,)1S lppuS( 011 ,59 91 ygolotisaraP sulporcim sulihpooB ... yrotarobaL AJ riaH ,DC kcirtaP 71 3 51- 5 41 ,23 ,09 91 aigolotissaraP lortnoc rof seigetarts dna skcit tsoh-itlum dna elgnis ni ecnatsiser ediciracA J naloN 61 633-523 ,02 ,89 91 lonummI etisaraP nietorpocylg...

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Báo cáo y học: "Attenuation of murine antigen-induced arthritis by treatment with a decoy oligodeoxynucleotide inhibiting signal transducer and activator of transcription-1 (STAT-1)" ppt

Danh mục: Báo cáo khoa học

... histological sections and U Griechen for technical assistance This work was supported by the Deutsche Forschungsgemeinschaft (Br 13 72/5 -1, Wi 11 02/8 -1 to Wi 11 02/8-3, and SFB405/B17), and the Interdisciplinary ... at the 66th Annual Scientific Meeting of the American College of Rheumatology, New Orleans, Lousiana, USA, October 2002 References 10 11 12 13 14 15 16 17 Cush JJ, Lipsky PE: Cellular basis for ... expression of STAT -1, STAT-3, IRF -1 and CD40 was measured by real time PCR The house keeping genes encoding β-actin (for STAT -1 and STAT-3) and GAPDH (for IRF -1 and CD40) were used for normalization of...

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Báo cáo y học: " Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34+ progenitor cells and derivation of HIV-1 resistant T cells and macrophages" pdf

Danh mục: Báo cáo khoa học

... Acad Sci USA 2003, 10 0 :18 3 -18 8 Page 10 of 11 (page number not for citation purposes) AIDS Research and Therapy 2004, 1: 2 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Anderson J, Banerjea ... flap sequence and the wood- Page of 11 (page number not for citation purposes) AIDS Research and Therapy 2004, 1: 2 http://www.aidsrestherapy.com/content /1/ 1/2 Figure HIV -1 challenge of in vivo differentiated ... control of the VA1 promoter (Fig 1C) Production of high titered retroviral vectors To generate vector stocks, 293T cells were transfected with 15 µg of pCHGP-2 (encodes HIV -1 gag/pol), 15 µg of transfer...

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Báo cáo y học: "Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus" pps

Danh mục: Báo cáo khoa học

... characterization of the DNA of Anatid herpesvirus Intervirology 19 93, 36:99 -11 2 Zhang et al Virology Journal 2 010 , 7:2 41 http://www.virologyj.com/content/7 /1/ 2 41 10 11 12 13 14 Page of Sandhu , Metwally ... Expression and characterization of the UL 31 protein from duck enteritis virus Virol J 2009, 6 :1 doi :10 .11 86 /17 43-422X-7-2 41 Cite this article as: Zhang et al.: Production, purification and characterization ... Zhang et al Virology Journal 2 010 , 7:2 41 http://www.virologyj.com/content/7 /1/ 2 41 Page of Figure Expression and purification of the tgK protein M represented standard protein molecular weight...

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Báo cáo y học: " Genital herpes evaluation by quantitative TaqMan PCR: correlating single detection and quantity of HSV-2 DNA in cervicovaginal lavage fluids with cross-sectional and longitudinal clinical data" doc

Danh mục: Báo cáo khoa học

... 0 .11 0.46 24 .18 24 .13 24 .15 0.04 0 .15 24 .12 23.74 23.93 0.27 1. 13 27.25 0 .16 0.57 30.76 0 .14 0.45 34.30 0.25 0.74 37. 41 0 .15 0. 41 15,000 27. 31 0.04 0 .14 27.36 0 .15 0.55 26.97 27 .16 27.07 0 .13 ... (24%) 11 (16 %) (20%) 10 (25%) Transfusion risk 10 (3%) (0%) (1% ) (3%) No identified risk 85(22%) (18 %) 40 (59%) 21 (53%) 0 -1 16 (4%) (5%) (4%) (3%) 2-4 51 (13 %) (23%) 13 (19 %) (15 %) 5-9 10 -50 81 ... Institute of Allergy and Infectious Diseases (UO1-AI-35004, UO1-AI- 318 34, UO1-AI-34994, UO1-AI-34989, UO1-AI34993, and UO1-AI-42590) and by the National Institute of Child Health and Page of Human...

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Báo cáo khoa học: "Expression, purification and characterization of the Lily symptomless virus coat protein from Lanzhou Isolate" doc

Danh mục: Báo cáo khoa học

... Strains, plasmids, and enzymes Expression and purification of LSV coat protein Figure Expression and purification of LSV coat protein A, Expression and purification of LSV CP 15 % SDS-PAGE of cultures ... 2007, 13 9 :12 5 -13 1 Allen , Lyons : Electron microscopy of lily symptomless virus and cucumber mosaic virus within fleck diseased lily Phytopathology 19 69, 59 :13 18 -13 22 Hsu HT, Kim JY, Lawson RH: Purification ... Virol 19 90, 71: 917 -924 11 Choi SA, Ryu KH: The complete nucleotide sequence of the genome RNA of Lily symptomless virus and its comparison with that of other carlaviruses Arch Virol 2003, 14 8 :19 43 -19 55...

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Báo cáo y học: "Association of chemokine receptor gene (CCR2CCR5) haplotypes with acquisition and control of HIV-1 infection in Zambians" pdf

Danh mục: Báo cáo khoa học

... 203 1. 1 0.85 -1. 46 0. 417 10 4 1. 1 0.77 -1. 56 0. 61 99 1. 2 0.75 -1. 75 0.5 31 HHD 16 7 1. 0 0.74 -1. 32 0.983 94 0.9 0.59 -1. 23 0.385 73 1. 2 0.75 -1. 92 0.442 HHE HHD/HHE 15 8 31 1 .1 1.9 0.82 -1. 51 1 .14 -3 .16 0.495 ... (15 .9) 75 (15 .6) 10 5 (16 .1) HHE 310 (13 .7) 13 8 (12 .2) 17 2 (15 .2) 61 (12 .7) 11 1 (17 .0) HHF *1 128 (5.6) 76 (6.7) 52 (4.6) 24 (5.0) 28 (4.3) HHF*2 480 ( 21. 2) 259 (22.8) 2 21 (19 .5) 99 (20.6) 12 2 (18 .7) ... HHA 6 01 (26.5) 305 (26.9) 296 (26 .1) 13 3 (27.7) 16 3 (24.9) HHB HHC 44 (1. 9) 17 8 (7.9) 25 (2.2) 69 (6 .1) 19 (1. 7) 10 9 (9.6) (1. 7) 42 (8.8) 11 (1. 7) 67 (10 .2) HHD 370 (16 .3 19 0 (16 .8) 18 0 (15 .9)...

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Báo cáo y học: "Conformational alterations in the CD4 binding cavity of HIV-1 gp120 influencing gp120-CD4 interactions and fusogenicity of HIV-1 envelopes derived from brain and other tissues" pptx

Danh mục: Báo cáo khoa học

... interests Page of 14 15 16 17 18 19 20 21 Received: 20 April 2 011 Accepted: June 2 011 Published: June 2 011 References Chan DC, Fass D, Berger JM, Kim PS: Core structure of gp 41 from the HIV envelope ... proportional to the amount of sequence divergence overlaps the gp120 CD4bs, using a subset of Envs (n = 12 , Macs1br2-8 and Macs1sp3 -15 ; see Figure 1) , as described previously [13 ,35] In these assays, ... in the CD4 binding cavity of HIV -1 gp120 influencing gp120-CD4 interactions and fusogenicity of HIV -1 envelopes derived from brain and other tissues Retrovirology 2 011 8:42 Submit your next manuscript...

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Báo cáo y học: " APOBEC3G-UBA2 fusion as a potential strategy for stable expression of APOBEC3G and inhibition of HIV-1 replication" pot

Danh mục: Báo cáo khoa học

... Page 11 of 13 (page number not for citation purposes) Retrovirology 2008, 5:72 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 innate immunity to retroviral infection Cell 2003, 11 3(6):803-809 ... Vif(-) HIV-1NL4-3 P24 antigen was measured post-infected day 3, 5, 7, 10 14 , 21 a results of wild type Vif(+) HIV-1NL4-3 infection; b results of Vif(-) HIV-1NL4-3 Δ Vif infection Page of 13 (page ... Nature 19 98, 3 91( 6668): 715 - 718 Lambertson D, Chen L, Madura K: Pleiotropic defects caused by loss of the proteasome-interacting factors Rad23 and Rpn10 of Saccharomyces cerevisiae Genetics 19 99, 15 3 (1) :69-79...

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Báo cáo y học: "Destabilization of the TAR hairpin leads to extension of the polyA hairpin and inhibition of HIV-1 polyadenylation" pptx

Danh mục: Báo cáo khoa học

... Mol Cell Biol 19 91, 11 :16 24 -16 30 Gilmartin GM, Fleming ES, Oetjen J: Activation of HIV -1 premRNA 3' processing in vitro requires both an upstream element and TAR EMBO J 19 92, 11 :4 419 -4428 Valsamakis ... no competing interests 12 13 14 15 16 17 18 Authors' contributions 19 MMV and AH performed the experiments MMV drafted the manuscript ATD and BB designed the experiments and revised the manuscript ... http://www.retrovirology.com/content/6 /1/ 13 10 11 rtTA assay HeLa X1/6 cells were cultured in 2-cm2 wells to 60% confluency and transfected with μg of the HIV-rtTA constructs and 0.5 ng pRL-CMV (Promega), in which the expression of...

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Bees and their role in forest livelihoods A guide to the services provided by bees and the sustainable harvesting, processing and marketing of their products

Danh mục: Cao đẳng - Đại học

... Marketing beeswax Making beeswax foundation 11 0 11 0 11 0 11 1 11 1 11 1 11 1 11 OTHER PRODUCTS FROM BEES Pollen Propolis Royal jelly Minor products 11 3 11 3 11 4 11 7 11 8 12 APITHERAPY Honey as medicine Naturally ... from? 12 Tips for honey marketing 13 Residue-free honey 14 Standard of contract in the trade of honey 15 General methods and terms of payment 16 Viral diseases 11 18 30 33 34 41 81 115 13 2 13 7 14 5 ... therapy 11 9 11 9 12 0 12 0 12 0 12 1 12 1 12 1 12 1 13 VALUE-ADDED PRODUCTS Value-addition Add profit by increasing product diversity Create employment for other sectors A way to use excess produce Costs of...

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Dynamics of epitaxial graphene growth and adsorptions of cobalt 1

Danh mục: Cao đẳng - Đại học

... distance of adatom from graphene plane (Å) Magnetic moment (B) Adatom to graphene charge transfer (e) Reference Hole 1. 27 2 .12 - 1. 31) - 81 Hole 1. 32 2.09 1. 56 1. 1 1. 1 83 Hole 0.97 - 1. 51 1.0 0.6 ... decomposition of silicon carbide (SiC) at above 12 00oC [6,7,8,9] and cracking of a carbon carrying gas on a metallic surface [10 ,11 ,12 ] Of all these methods, the solid-state decomposition of SiC is ... Si Layer BL [00 01] Layer Top view (d) 60o A B off-bond (0.63 Å) A 15 .12 Å C (e) on-bond C 7.56 Å (1. 89 Å) [00 01] B [11 00] A 3.08 Å Top view Top view Fig 1. 2 (a) A single unit of Si-C structure;...

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