handling of samples dna extraction and pcr amplification silva et al 2007 walker and rapley 1999
Báo cáo y học: "Detection of Chlamydia trachomatis-DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems" pps
... elsewhere) Materials and methods Ethical approval Before initiation of the study ethical approval was obtained by the ethical committee of Hannover Medical School, Germany Synovial fluid samples During ... systems allowed higher sensitivity than PCR regardless of the extraction method employed The DNA extraction methods alkaline lysis and Qiaex II Gel Extraction Kit® + CTAB allowed almost equal sensitivity ... samples, culture of C trachomatis and performed DNA extraction, PCR analysis and drafted the manuscript IB and SM performed parts of DNA extraction as well as parts of PCR analysis Additionally IB performed...
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báo cáo khoa học: " DNA ligase 1 deficient plants display severe growth defects and delayed repair of both DNA single and double strand breaks" potx
... slower phase in which the small remainder of DNA damage was repaired The initial rapid removal of the majority of DSBs from genomic DNA followed first order kinetics Analysis of the first ten minutes ... alkali/neutral version of comet assay and evaluated for comet formation The mean percentage of DNA in the comet tail for 300 comets for each concentration of MMS are shown Induction of SSBs is linear ... cycle delay or arrest and activation of DNA repair pathways [3] DNA ligases play essential roles in all organisms by maintaining the physical structure of DNA These enzymes seal gaps in the sugar-phosphate...
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qinghua et al - 2007 - audit committee, board characteristics and quality of financial reporting - an empirical research on chinese securities market
... (Beasley, 1996; Kao and Chen, 2004), negative relation ( Xie et al. , 2003; Anderson et al. , 2004) and no relation (Bardard et al. , 2004; Abbott et al. , 2004), contingent upon practical situations As ... Conclusions and limitations This paper explored the interrelationship between board characteristics and the quality of financial reporting by means of theoretical analysis and empirical testing ... earnings (the mean of DAt / At-1 is negative) Also, the maximum value and minimum value of DAt / At-1 are 1.45 and −1.07 respectively (i.e 1.45 and 1.27 times of companies’ total assets), indicating...
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gul et al - 2007 - auditor independence - evidence on the joint effects of auditor tenure and non-audit fees
... Francis and Ke 2002; Frankel et al 2002; DeFond et al 2002; Ashbaugh et al 2003; Chung and Kallapur 2003; Larcker and Richardson 2004) Findings of these studies are mixed Frankel et al (2002) ... conduct analyses on different cut-off points Association between Discretionary Accruals and Non-audit Fees We evaluate the association between discretionary accruals and audit fee metrics using ... we use the log of audit fees, the log of non-audit fees, the log of total fees and the ratio of non-audit fees to total fees (FEERATIO) as fee metrics The use of these metrics allows us to compare...
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color atlas of histopathology of the cervix uteri 2nd ed - c. dallenbach - hellweg, et al., (springer, 2006)
... occasionally also present in upper epithelial layers of severe reactive and inflammatory change (al- Saleh et al 1995; Bulten et al 1996; Kruse et al 2001; Pirog et al 2002) The expression of CEA ... cell (tubal) metaplasia of endocervical glands H&E Heterotopic Ectodermal and Mesodermal Structures Heterotopic Ectodermal and Mesodermal Structures (Figs 43–47) Rarely, heterotopic “neometaplasia” ... study of all margins of the conization site, of both parametrial tissues and all lymph nodes surgically excised For fixation, a 4% neutral solution of formaldehyde is commonly used and is ideal...
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encycopedia of materials characterization surfaces interfaces thin films c brundle et al bh 1992 pdf
... standards, and chemical state analysis, for materials as diverse as biological to metallurgical X P S also is known as electron spectroscopy for chemical analysis (ESCA) Range of elements All ... characterization methods began about 150 years ago when thin sections of rocks and minerals, and the first polished metal and metal-alloy specimens were prepared and viewed with the intention of correlating ... crystals of conductors and semiconductors; insulators and polycrystalline samples under special circumstances; 0.25 cm2 or larger, smaller with special effort Main uses Analysis of surface crystallography...
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Encycopedia of Materials Characterization (surfaces_ interfaces_ thin films) - C. Brundle_ et al._ (BH_ 1992) WW Part 2 pps
... general and universal There are microscopical atlases for the identification of metals and alloys,' rocks and ores,2paper fibers, animal feeds, pollens, foods, woods, animal hairs, synthetic ... other profilometers STM and SFM are excellent high-resolution profilometers STM and SFM are free from many of the artifacts that afflict other kinds of profilometers Optical profilometers can ... surface of a magnetic sample The orientation of the magnetization is determined from the sign of the rotation and the geometry of the setup MOKE measurements can be made as a function of external...
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Encycopedia of Materials Characterization (surfaces_ interfaces_ thin films) - C. Brundle_ et al._ (BH_ 1992) WW Part 3 pps
... The main advantages of EDS are its speed of data collection; the detector’s e%ciency (both analytical and geometrical);the ease of use; its portability; and the relative ease of interfacing to ... Materials Research Society It Conclusions TEM is an established technique for examining the crystal structure and the microstructure of materials It is used to study all varieties of solid materials: ... mathematical expression that has the shape of a Gaussian curve offset at the surface of the sample This function is often called the $(pz) hnction The lateral resolution of X-ray analysis in...
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Encycopedia of Materials Characterization (surfaces_ interfaces_ thin films) - C. Brundle_ et al._ (BH_ 1992) WW Part 5 ppt
... understanding of chemical and structural properties of surfices and interfaces XPD and AED offer both with a relatively small investment in time and equipment, if one is using XPS or AES already ... studies of passive films have been on chemically passivated metals: Electrochemically passivated metals are of greater technological significance In addition, the structures of passive films &et attack ... evaporated film of Pd on Sill 111 and for bulk palladium silicide, P&Si and metallic Pd SEXAFS Data Analysis and Examples Often a comparison of raw data directly yields usell structural information...
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Encycopedia of Materials Characterization (surfaces_ interfaces_ thin films) - C. Brundle_ et al._ (BH_ 1992) WW Part 6 ppsx
... generally used of the truly surface sensitive techniques, being applied now routinely for elemental and chemical state analysis over a range of materials in a wide variety of technological and ... WNDUCTlON BAND - -VALENCE BAND NE) B.E Figure CEELS VEELS Representation of a typical density of electron statesfor a metal having X, V and Zcore levels [top); and REELS spectrum expected from metal ... strengths of XPS are its good quantification, its excellent chemical state determination capabilities, its applicability to a wide variety of materials from biological materials to metals, and its...
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Encycopedia of Materials Characterization (surfaces_ interfaces_ thin films) - C. Brundle_ et al._ (BH_ 1992) WW Part 7 ppt
... enhancements and absorptions, etc Two general methods used for making these corrections are the empirical parameters method and the fundamental parameters methods The empirical parameters method uses ... variety of internal and external parameters, such as chemical composition, temperature, strains, and electric and magnetic fields The line widths are a function of the quality of the material, ... general name of fluorometry In PL of semiconductors, one monitors emission from the bottom of the conduction band to the top of the valence band All the properties that can be determined from band-edge...
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Encycopedia of Materials Characterization (surfaces_ interfaces_ thin films) - C. Brundle_ et al._ (BH_ 1992) WW Part 8 pptx
... coefficients 01 and p, and thus Y and A, can then be determined By changing the angle of incidence and wavelength, the user can determine N sets of Y j and Ai values for the regression analysis used ... controlled.u The geometry of a typical ellipsometry set up is shown in Figure Ellipsometry is a very powerfd, simple, and totally nondestructivetechnique for determining optical constants, film ... Ishida and A Ishitani) review microscopicand surf$ce analytical techniques Chapter (by D M Haaland) reviews developments in statisticalchemometricsh data analysis r 0.S Heavens OpticalProperticsofThin...
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Encycopedia of Materials Characterization (surfaces_ interfaces_ thin films) - C. Brundle_ et al._ (BH_ 1992) WW Part 9 pps
... interconnect I 111-Vand11-VImaterials(AlxGal&, andHg,Cdi-%Te) Metal multilayer stacks DoseAattice substitutionality of implanted species Crystal damage versus depth (Si, SiGe,, Al, Gal-As, and Hg$dl-xTe) ... 200,300,400, and 500 MHz, respectively The spectrometer console comprises a radiofrequency part for the generation, amplification, mixing, and detection of radiofrequency and NMR signals, and a digital ... = Dreal x x qeal (3) where T& and hS the thickness obtained by RBS and the density assumed are to calculate this thickness; and T d and Drd are the actual physical thickness and density of the...
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Encycopedia of Materials Characterization (surfaces_ interfaces_ thin films) - C. Brundle_ et al._ (BH_ 1992) WW Part 10 pdf
... 10.3 SAL1 563 Common Modes of Analysis and Examples SALI applies two methods of post-ionization, MPI and SPI, each of which can be used in one of the three modes of analysis: survey analysis, ... and microanalytical techniques for materials characterization It is primarily used in the analysis of semiconductors,as well as for metallurgical, and geological materials The advent of a growing ... transients SALI has been applied to two basic groups of samples: inorganic and organic solid materials For inorganic analysis or elemental analysis (e.g., semiconductors and metal alloys), ionization...
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Encycopedia of Materials Characterization (surfaces_ interfaces_ thin films) - C. Brundle_ et al._ (BH_ 1992) WW Part 11 pps
... a diameter of 1.5-2.0 mm and a length of 18-22 mm Indium and Gallium Metal Table summarizes the results of an analysis to the 6N-7N total impurity level of very high purity In and Ga metals, such ... The analysis of a material in limited supply, and when too little is available for analysis by alternative methods A number of examples of the application of GDMS to various metals and alloys ... experiments, the determination of elemental contaminants in actual failures is a difficult analytical problem, in particular because of the small size and complex geometry of the parts Figures and illustrate...
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Encycopedia of Materials Characterization (surfaces_ interfaces_ thin films) - C. Brundle_ et al._ (BH_ 1992) WW Part 12 pot
... depends on the number ofsamples to be analyzed per day, the number of elements of interest, whether the analysis will be ofsimilar samples o of a wide range of samr ple types, and whether the chosen ... few millimeters of metal are required to assure the safety of workers near an X-ray source, several meters of absorbing material (usually steel, concrete, or boron-containing materials) are required ... neutron magnetic moment strongly interacts with the magnetic moment of the sample atoms, allowing determination of the spatial arrangements of magnetic moments in magnetic material The equivalent interaction...
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Encycopedia of Materials Characterization (surfaces_ interfaces_ thin films) - C. Brundle_ et al._ (BH_ 1992) WW Part 13 pdf
... The use of chemisorption is confined to the measurements of metal surface areas of finely divided metals, such as powders, evaporated metal films, and those found in supported metal catalysts ... optical profilometer are compared to those from scatterometerand stylus measurements and discussed in Jacobson et aL4 The optical profdometer equipped with a 20x objective lens has a short spatial ... Ir and Pt) Finally, the same reaction was studied with a series of unsupported Ni:Cu In all cases, the total surface areas (equal to the metal area for an unsupported metal) of the pure catalysts...
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