giới thiệu tổng quan về chi nhánh
Cloning, expression and characterization of oxysterol binding protein homologue 7 (OSH7) in yeast saccharomyces cerevisiae
Ngày tải lên :
03/10/2015, 20:57
... it possible for me to finish this project I would also like to express my sincere gratitude to Chieu Hai Kee for her effort contributed to this project as well as her valuable suggestions in ... project 44 Compositions of SDS-PAGE 57 11 LIST OF ABBREVIATIONS aa amino acid Amp ampicillin CHO Chinese Hamster Ovary CPY carboxypeptidase Y DTT dithiothretiol EDTA ethylene diamine tetra-acetic ... domain at the N-terminus The localization of OSBP within cells is governed by lipids In transfected Chinese hamster ovarian cells (CHO) overproducing OSBP, the OSBP is found to be distributed diffusely...
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KRONE - What is Patch by Exception
Ngày tải lên :
26/10/2013, 19:15
... reductions common mounting can be achieved using existing LSA-PLUS mounting, and does not require expensive "19 inch" accommodation such as racks and cabinets When patching is required, it can be carried ... solution significantly reduces the amount of patching required as most of the moves and changes are handled at the software level In the instances when patching is required, changes can be administered ... When patching is required, it can be carried out reliably using latched patch plugs, and can be achieved without any interruption to live circuits by virtue of the "make before break" feature of...
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Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf
Ngày tải lên :
15/02/2014, 01:20
... Kohama K, Fukushima T, Miyata S, Uchiyama S, Uchinokura S, Shimomura T et al (2005) Hepatocyte growth factor activator inhibitor type (HAI-1) is required for branching morphogenesis in the chorioallantoic ... of the protease domain was quantified using the protease domain of the protein obtained from E coli Enzyme inhibition assay HAI-1–K1 was prepared as follows Escherichia coli cells were transformed ... fraction was treated overnight with enterokinase at unitsÆ100 lL)1 Quantification of TMPRSS13 The enterokinase-treated pro-TMPRSS13 was quantified by immunoblotting using the protein expressed in E coli...
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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
Ngày tải lên :
18/02/2014, 04:20
... system was originally identified as machinery contributing to multivesicular endosome (multivesicular body) formation in the endocytic pathway [24,25] ESCRT machinery has been proposed to have additional ... system Physiol Rev 83, 731–801 4424 Sorimachi H & Suzuki K (2001) The structure of calpain J Biochem 129, 653–664 Suzuki K, Hata S, Kawabata Y & Sorimachi H (2004) Structure, activation, and biology ... S12–S18 Maki M, Kitaura Y, Satoh H, Ohkouchi S & Shibata H (2002) Structures, functions and molecular evolution of the penta-EF-hand Ca2+-binding proteins Biochim Biophys Acta 1600, 51–60 Moldoveanu...
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Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
Ngày tải lên :
18/02/2014, 12:20
... Glutathione–Sepharose, pGEX-2T vector, thrombin, [methyl-3H]dThd (25 CiÆmmol)1) and the Escherichia coli strains XL Gold and BL21 were purchased from Amersham Biosciences (now part of GE Healthcare ... synthesis Annu Rev Biochem 57, 349– 374 Pontarin G, Ferraro P, Valentino ML, Hirano M, Reichard P & Bianchi V (2006) Mitochondrial DNA depletion and thymidine phosphate pool dynamics in a cellular model...
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Tài liệu Báo cáo khoa học: Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens 1 (PDZ) scaffold multi-PDZ domain protein 1 pptx
Ngày tải lên :
18/02/2014, 13:20
... C-terminus GST fusion proteins and mutant construct thereof (trunc8-GST) were produced in Escherichia coli XL1 blue and purified on glutathione sepharose beads (Becton-Dickinson Biosciences, Franklin ... 575–586 11 Paysan J & Breer H (2001) Molecular physiology of odor detection: current views Pflugers Archiv – Eur J Physiol 441, 579–586 12 Tsunoda S & Zuker CS (1999) The organization of INAD-signaling ... supramolecular complexes Ann Rev Neurosci 24, 1–29 16 Songyang Z, Fanning AS, Fu C, Xu J, Marfatia SM, Chishti AH, Crompton A, Chan AC, Anderson JM & Cantley LC (1997) Recognition of unique carboxyl-terminal...
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Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx
Ngày tải lên :
18/02/2014, 18:20
... nuclear lysate compared to HeLa whole cell lysate Due to the commercial availability of suitable quantities of HeLa nuclear pellets, these were chosen as the starting material for a purification ... of the resulting fractions were measured, and a final purification of approximately 939-fold was achieved Purification by butyl-sepharose chromatography resolved the majority of the Y17 kinase activity ... Fisher Scientific, Runcorn, UK), CDK1 phospho-Y15 (Cell Signaling Technology, New England Biolabs, Hitchin, UK), aTubulin DM1A (Sigma-Aldrich) and C-YES (BD Biosciences, Oxford, UK) Immunoprecipitation...
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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
Ngày tải lên :
19/02/2014, 00:20
... Science Council, Taiwan, Republic of China (NSC 892320-B-006-113, NSC 90-2314-B-006-123, NSC 932320-B-309-002 and NSC 95-2313-B-309-001) References Chen TR, Hsiao MH, Chiou CS & Tsen HY (2001) Development ... Dynatech, MeLean, VA, USA) 96-well plate reader at a wavelength of 450 nm Cytokine levels were quantified by comparison with standards The sensitivity of IL-1b and IL-6 was < 0.1 and < 0.7 pgÆmL)1, ... the cells treated with ngÆmL)1 of SEC1 for 24 h The NF-jB-DNA binding activity (lanes 2–8) was quantified by densitometry The time-course groups were compared with the Ctl group to obtain the...
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Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf
Ngày tải lên :
19/02/2014, 06:20
... arrangement of DNA helices, allowing the efficient catenation of the circular duplexes by Escherichia coli topoisomerases, the free exchange of DNA between neighboring aggregates by the enzyme ... residual molecules in the supernatant (Fig 1C, upper panel) The increasing amounts of extracts quantitatively precipitated the DNA, ending up with saturation at a full extent (lanes and 14) Considering ... (ppt.) and its supernatant (sup.) were resolved on an agarose gel Maximal aggregation of DNA was achieved by using either 5.4 lg of nuclear fraction, 1.0 lg of DNA affinity fraction or 0.7 lg of...
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Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf
Ngày tải lên :
20/02/2014, 02:21
... vitro transcription of these gene copies in PSG nuclear extracts is shown at the bottom and the quantified transcription levels as the percentage of tRNA1Gly -1 taken as 100, are indicated on the ... activity Evidently, the fractions were devoid of mutual contamination In every fractionation the quantities of fractions had to be optimized because use of larger amounts of any individual fraction ... the interaction of TFIIIB with tRNA1 -1 was Gly more stable than the interaction with tRNA1 -6,7 Quantification of the ratio of heparin-resistant complexes to the TFIIIB ⁄ C–DNA complexes in the...
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
Ngày tải lên :
20/02/2014, 11:20
... constitutive or induced, remain unclear A comprehensive understanding of the general transcription machinery on the native fgl2 promoter is necessary for developing further insight into the TF-independent ... after intraperitoneal administration of 1.5 mL of 5% thioglycollate (Difco Laboratories, Detroit, Michigan) as previously described [29] Macrophages were greater than 95% pure as determined by morphology ... (Eur J Biochem 270) 2279 Fig Functional analysis of fgl2 promoter (A) Analysis of fgl2 RNA by semiquantitative RT-PCR RT-PCR was performed with lg of each RNA sample M, macrophages; M + MHV-3, macrophages...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
Ngày tải lên :
21/02/2014, 01:21
... manganese-superoxide dismutase of Escherichia coli J Biol Chem 266, 303–308 Ose, D.E & Fridovich, I (1979) Manganese-containing superoxide dismutase from Escherichia coli: reversible resolution and ... F., Schinina, M.E., Bannister, W.H & Bannister, J.V (1987) Structural identity between the iron- and manganese-containing superoxide dismutases Protein Eng 1, 393–397 Meier, B., Sehn, A.P., Schinina, ... Escherichia coli Biochemistry 36, 4925–4933 25 Edwards, R.A., Whittaker, M.M., Whittaker, J.W., Baker, E.N & Jameson, G.B (2001) Removing a hydrogen bond in the dimer interface of Escherichia coli...
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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
Ngày tải lên :
22/02/2014, 04:20
... envelope EMBO J 15, 7108–7119 Kawahire, S., Takeuchi, M., Gohshi, T., Sasagawa, S., Shimada, M., Takahashi, M., Abe, T.K., Ueda, T., Kuwano, R., Hikawa, A., Ichimura, T., Omata, S & Horigome, T (1997) ... binding of LBR to chromatin (Eur J Biochem 269) 953 28 Kawahire, S., Tachibana, T., Umemoto, M., Yoneda, Y., Imai, N., Saito, M., Ichimura, T., Omata, S & Horigome, T (1996) Subcellular distribution ... K252b (compare columns 3–6 with column in Fig 6A) Fifty percent suppression with staurosporine was achieved with as little as nM (data not shown) These results indicate that the increase in the...
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Tài liệu Báo cáo Y học: Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents pptx
Ngày tải lên :
22/02/2014, 07:20
... the quantification of changes in mRNA levels of 23 apoptosis-related genes in chorion laeve tissue during in vitro incubation of human fetal membranes obtained at term Based on the extent of quantitative ... T4 Nature 227, 680–685 12 Bradford, M.M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, ... glutathione peroxidase and glutathione reductase were also expressed constitutively and showed no quantitative alteration We provisionally named this group of genes group-1 Expression of mRNA for...
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Tài liệu Báo cáo Y học: Toxicity of substrate-bound amyloid peptides on vascular smooth muscle cells is enhanced by homocysteine docx
Ngày tải lên :
22/02/2014, 07:20
... Three fields in each well were photographed with an Olympus DP10 digital camera and apoptotic nuclei quantified Cells with condensed or fragmented nuclear chromatin were considered apoptotic The number ... [45] who found that substratebound Ab inhibited neurite outgrowth and cell adhesion Interestingly, Chinese hamster ovary cells transfected with a5b1 integrin demonstrated reduced susceptibility to ... associated with Alzheimer’s disease patients [64] and other disorders of the nervous system such as schizophrenia and Parkinson’s disease Homocysteine has also been shown to be toxic to neurons in...
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Báo cáo khoa học: Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages pdf
Ngày tải lên :
06/03/2014, 22:21
... protein expression was analyzed by western blotting and relative signal intensities were quantified using Quantity One Software The results are representative of four independent macrophage preparations ... kit (Amersham) and the intensity of signals was subsequently analyzed by densitometry and quantified using Quantity One software Measure of visfatin protein secretion by ELISA Human RM were treated ... Y, Murakami M, Ichisaka T, Murakami H et al (2007) Retraction Science 318, 565 14 Fukuhara A, Matsuda M, Nishizawa M, Segawa K, Tanaka M, Kishimoto K, Matsuki Y, Murakami M, Ichisaka T, Murakami...
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Báo cáo khoa học: Autocatalytic processing of procathepsin B is triggered by proenzyme activity doc
Ngày tải lên :
07/03/2014, 03:20
... investigated by zymography Recombinant human procathepsin B and cathepsin B were produced in Escherichia coli and thus represented nonglycosylated enzymes Initially, procathepsin B, cathepsin B and ... phosphate buffer (pH 6.0) containing mm EDTA and 0.1% (w ⁄ v) polyethylene glycol 6000 (Serva, Wichita Falls, TX, USA) Fluorescence of the released 7-amino-4-methylcoumarin was followed continuously ... References Turk B, Turk D & Turk V (2000) Lysosomal cysteine proteases: more than scavengers Biochim Biophys Acta 1477, 98–111 Turk V, Turk B & Turk D (2001) Lysosomal cysteine proteases: facts...
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Báo cáo khoa học: The autophagic response to nutrient deprivation in the hl-1 cardiac myocyte is modulated by Bcl-2 and sarco⁄endoplasmic reticulum calcium stores ppt
Ngày tải lên :
07/03/2014, 09:20
... deconvolution (AutoQuant) Scale bar, 10 lm (B) The percentages of cells with numerous GFP–LC3 punctae without (steady-state, solid bar) and with lysosomal inhibitors (cumulative, hatched bar) were quantified ... 59 Demarchi F, Bertoli C, Copetti T, Tanida I, Brancolini C, Eskelinen EL & Schneider C (2006) Calpain is required for macroautophagy in mammalian cells J Cell Biol 175, 595–605 60 Demarchi F, ... and autophagosome–lysosome fusion [24] Baf effectively blocked lysosomal acidification (Fig 1B) Quantifying autophagy and autophagic flux in HL-1 cardiac myocytes We first characterized the basal...
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Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx
Ngày tải lên :
07/03/2014, 16:20
... Talamillo, A., Chisholm, A.A., Garesse, R & Jacobs, H.T (1998) Expression of the nuclear gene encoding mitochondrial ATP synthase subunit alpha in early development of Drosophila and sea urchin Mol ... Mathieu-Costello, A., Tsuchiya, N., Filburn, C & Hansford, R.G (1997) Mitochondrial biogenesis during cellular differentiation Am J Physiol 272, C1345–51 Chung, A.B., Stepien, G., Haraguchi, Y., Li, K & ... expression of genes that are essential for the cell-cycle and for the nuclear DNA replication machinery [26], establishing a link between mitochondrial and nuclear DNA replication [24,25] Here,...
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Báo cáo khoa học: Androgen receptor function is modulated by the tissue-specific AR45 variant ppt
Ngày tải lên :
07/03/2014, 16:20
... Solloway M, Prall O, Stennard F, Schindeler A, Groves N, Lavulo L, et al (2002) Homeodomain factor Nkx2–5 in heart development and disease Cold Spring Harb Symp Quant Biol 67, 107–114 35 Valen ... AR45–GFP expression construct and treated with R1881 Fluorescence microscopy was used to visualize the chimeric AR45 protein Right panel: nuclear staining with DAPI FEBS Journal 272 (2005) 74–84 ª 2004 ... transfected with 20 lg of an AR45-expressing vector The number of viable cells was measured after days by quantifying the ATP levels (grey bars) In the control experiment empty pSG5 plasmid was used (white...
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