gfp aequorin probes in tissue slices and animals

... artificial tissue and organ replacements This is the field of tissue engineering, which has been defined as "an interdisciplinary field that applies the principles of engineering and the life ... surrounding tissues, and which replaces the original missing tissue This is in contrast to prosthetic devices, which are designed to maintain their mechanical integrity during their intended ... for Tissue Engineering Applications 33 3.1 Introduction As explained in chapter 1, inadequate vascularization is a major bottleneck limiting widespread clinical applications of tissue engineering,...

Ngày tải lên: 09/09/2015, 11:11

... proline and hydroxyproline As a non-essential amino acid, proline is synthesized from arginine/ornithine via the urea cycle and glutamate (directly or indirectly from glutamine via glutaminase) ... protein samples were pooled and resolved under non-reducing conditions using in- house 5% resolving/3% stacking polyacrylamide gels on a Mini-Protean as outlined in Raghunath et al [93] Protein standards ... proline into the wound [78] Providing additional proline or glutamine in the diet to enhance collagen biosynthesis, however, does not result in increased collagen accumulation In contrast, arginine,...

Ngày tải lên: 10/09/2015, 15:52

... cylinders using a Brendel-Vitron tissue slicer [26] Tissue incubation Three lung slices from each donor were floated onto titanium roller inserts, blotted, and loaded horizontally into glass scintillation ... holes, placed in a Dynamic Organ Culture incubator (Vitron, Inc., Tucson, AZ, USA) at 37°C, and exposed to 95% O2 and 5% CO2 The human tissue slices were incubated for 2, 6, and 24 h, and cellular ... (ATP) content and protein synthesis in human lung tissue ATP content was measured using a luciferin-luciferase assay [27] Tissue slices were homogenized in mL 10% trichloroacetic acid and were immediately...

Ngày tải lên: 12/08/2014, 15:21

... pancreatic juice and intestine: inadequate for role in zinc absorption Am J Clin Nutr 35, 1–9 Evans GW & Johnson PE (1980) Characterization and quantitation of a zinc binding ligand in human milk ... Residues involved in catalysis in the bacterial enzyme and the corresponding amino acids in the human protein are pointed with arrows The mean hydrophobicity values of residues within the window ... of Huntington’s disease are duplicated in experimental animals by intrastriatal quinolinate injection [5,6] In addition, a significant elevation of quinolinate levels has been observed in low-grade...

Ngày tải lên: 19/02/2014, 02:20

... mRNA in muscle and heart were 6–24 times higher than in brain, eye, gills, testis, skin, kidney and swimbladder, and 320–650 times higher than in liver, intestine and ovary Both RT-PCR and RT-qPCR ... only detected in the olfactory vesicles of embryos and larvae (Fig 5), and in the liver, intestine, muscle, brain, heart, eye, gills, ovary, testis, skin, kidney and swimbladder in adult zebrafish ... Intracellular lipid-binding proteins and their genes Annu Rev Nutr 17, 277–303 Schaap FG, van der Vusse GJ & Glatz JFC (2002) Evolution of the family of intracellular lipid binding proteins in...

Ngày tải lên: 16/03/2014, 00:20

... Radiation in Tissue Banking Trim Size: 9in x 6in Need for Radiation Sterilization of Tissue Grafts ch02 15 contamination originating from the environment and handling Additional handling during processing ... Radiation in Tissue Banking Trim Size: 9in x 6in This page intentionally left blank ch03 FA March 15, 2007 22:5 b478 Radiation in Tissue Banking Trim Size: 9in x 6in Chapter Tissue Banking in the ... Radiation in Tissue Banking Trim Size: 9in x 6in This page intentionally left blank ch02 FA March 15, 2007 22:5 b478 Radiation in Tissue Banking PART II TISSUE BANKING Trim Size: 9in x 6in ch03...

Ngày tải lên: 22/03/2014, 20:21

... n-mers having a subsequence belonging to one file and the remaining sequence in another file were not included in our calculations All calculations on the human genome utilized both the original and ... properties of short subsequences in microbial identification In Proceedings of the International Conference on Mathematics and Engineering Techniques in Medicine and Biological Sciences (Valafar ... the sequences that are present in the genome of interest and absent from the host genome as being ‘host-blind’ (human-blind, mosquito-blind, mouseblind, rat-blind, etc.) sequences The greater...

Ngày tải lên: 23/03/2014, 11:20

... saline containing 5% dimethylsulfoxide and 1% methyl carboxy cellulose, and administered through i.p injections Epinephrine in physiological saline was also injected i.p Animals were housed in ... by low doses of norepinephrine exerted a significant inhibition on lipoysis (Fig 1C) The initial step involved in the epinephrine ⁄ norepinephrine-induced lipolytic cascade includes activation ... in the plasma via the lipoprotein system [5,6] Tissue- distribution studies have shown that B[a]P accumulates in lipid-storing tissues including the mammary glands and adipose tissue The carcinogenicity...

Ngày tải lên: 30/03/2014, 11:20

... The following gives an overview over the motifs mapped in different nuclear proteins, including transcription factors, DNA- and RNA-binding proteins, viral proteins, kinases, and kinase adapters ... transactivation domain, and a C-terminal steroid-binding domain While in these early studies the domain required for nuclear matrix attachment was localized to the C-terminal steroid binding domain in hAR, ... movement is determined by Ran, a small GTP-binding protein (reviewed in refs 22, 48 and 50) NPC structure includes soluble proteins, termed nucleoporins (nups) and integral membrane proteins, termed...

Ngày tải lên: 08/04/2014, 12:52

... hunting and foraging intelligences, courting and mating intelligences, flying and swimming intelligences, migrating and molting intelligences, communicating and playing intelligences—we begin to ... that in uenced the wording of these principles Biotechnology and Animals: Ethical Issues in Genetic Engineering and Cloning bernard rollin Since scientific ideology distances itself from ethics in ... in these stories is largely pragmatic and rural; animals are for food and work However, it is also a world in which imaginary and fantastic animals have a large share, and in which ordinary animals...

Ngày tải lên: 11/06/2014, 14:46

... results, drafting and revising the manuscript, and approving the manuscript in its final form RA, KK, and JO contributed to acquiring and managing the data, revising the manuscript, and approving the ... analyzing the data and interpreting the results, revising the manuscript, and approving the manuscript in its final form Competing interests The authors declare that they have no competing interests ... the manuscript in its final form EA contributed to performing statistical analysis, acquiring and managing the data, revising the manuscript, and approving the manuscript in its final form RT contributed...

Ngày tải lên: 20/06/2014, 22:20

... discriminate between the intentional shortening of life as a by-product of the pain therapy (administration of opiates such as morphine), inducing terminal sedation, terminating of life (e.g by administration ... “cripples” and other “unfit” persons and arguing that the doctrine of evolution justifies shortening the lives of suffering people (Vanderpool, 2004) Euthanasia and medicine in the 19th and 20th ... the overpopulation of animals In veterinary medicine euthanasia is routinely XI XII Preface used in the form of killing out of kindness severely injured and/ or seriously sick animals To not euthanatize...

Ngày tải lên: 28/06/2014, 04:20

... Standing Moral standing indicates those individuals that moral agents must respect in certain separately determined ways In Western countries all human beings are assumed to have moral standing ... guidelines demonstrate moral standing—the individual, though to be killed, must be managed (and killed) in a certain manner In contrast, it has been and remains common in the West to deny moral standing ... that having interests is synonymous with having inherent value If something can be in an entity’s interest, then that entity has inherent value: “A sentient being has intrinsic value in that...

Ngày tải lên: 05/08/2014, 21:23

... fields is critical both to determine and to support any given moral outlook, but incorrect information can be misleading Diligence in exploring and incorporating information from outside the field ... which (1) they pursue and obtain what they prefer, (2) they take satisfaction in pursuing and obtaining what they prefer, and (3) what they prefer and obtain is in their interests (Case 117) An animal ... disciplines; therefore this book is interdisciplinary, including such topics as biology and theology, ethology and economics Human endeavors are rooted in human “knowledge”; our understanding of...

Ngày tải lên: 05/08/2014, 21:23

... plainly stated intent and the whole point of utilitarianism Singer does not wish to increase but to decrease suffering by maximizing the satisfaction of interests “Singer is in favor of increasing ... possibilities embedded in Singer’s replaceability argument These surprising outcomes appear to stem from errors in Singer’s reasoning In “Killing Humans and Killing Animals Singer examines two utilitarian ... death and suffering Singer asserts that the ethics involved in killing are “much more complicated” than the morality of in icting suffering (Singer, Animal 228) For Singer, pain is pain, but the harm...

Ngày tải lên: 05/08/2014, 21:23

... intentions and aims must be directed toward not interfering with or harming animals and plants in natural ecosystems and to preserving their wild status for their sake Having those aims and intentions ... undesirable intrusion into the natural world c Fidelity Fidelity forbids gaining an entity’s trust in order to deceive and gain advantage for immoral reasons We are required to maintain integrity in our ... Regrettably, Taylor indicates, this is sometimes necessary He points to the hunting of whales and seals in the Arctic and the hunting of wild goats and sheep in mountainous regions In these cases,”...

Ngày tải lên: 05/08/2014, 21:23

... man and woman: And God said, “Let the earth bring forth living creatures of every kind: cattle and creeping things and wild animals of the earth of every kind.” And it was so God made the wild animals ... all things in him, things in heaven and things on earth (Eph 1:9–10) 246 chapter five [F]or in him all things in heaven and on earth were created all things have been created through him and ... creatures of God Linzey asserts that a good and loving God must remain morally responsible for and invested in every sentient being: “[A] redeeming God could not eschew the sighing and suffering of all...

Ngày tải lên: 05/08/2014, 21:23

... themselves in every other living being, and to see every other living being in themselves Inasmuch as Indians see God in humanity, they must also find the divine in anymals “Hindu belief in the cycle ... shining stars!/Praise him, you highest heavens, /and you waters above the heavens! /Mountains and all hills,/fruit trees and all cedars!/Wild animals and all cattle,/creeping things and flying ... “causes injury to many beings [T]he wooden implement with iron point injures the earth and the beings living in the earth” (Embree, Hindu 94) For many Indians, profiting from anymal suffering is...

Ngày tải lên: 05/08/2014, 21:23

... process “binds all things into one, equalizing all things and all opinions” (Chan 177) Nothing lies outside of this Daoist continuum, and so “the chain of being is never broken,” and a link can ... not fish or hunt and thereby harm and kill living beings You should not in winter dig up hibernating animals and insects You should not use cages to trap birds and [other] animals (Schipper ... skins of wild animals, against target practice on living creatures, and against inciting animals to fight for human entertainment A well-known Hadith story about a woman condemned for confining and...

Ngày tải lên: 05/08/2014, 21:23

... human being is not viable, even if a human being feels no pain and has no brain waves, even if a human being is terminally ill and in terrible pain, and even if a human life can only be maintained ... distinguishes between being interested in (active), versus something being in one’s interest (passive) (Narveson, Nature 22) Children are often interested in things that are not in their interest; ... her interests should receive” (249) Philosophers (including Regan and Singer) tend to include consciousness in their assessment of moral standing, generally favoring anymals with larger brains and...

Ngày tải lên: 05/08/2014, 21:23