... PEP-1-SOD1 and PEP-1-CAT fusion protein pET15b-SOD1-His, pET15b-PEP-1-SOD1-His, pET15bCAT-His and pET15b-PEP-1-CAT-His were successfully expressed and purified as shown in Figure 1A The results indicated ... individual proteins on LDH release and apoptosis rates in cardiomyocyte H9c2 cells The levels of LDH in the supernatants and the apoptosis of cells were indicators of hypoxiareoxygenation injury [25,26] ... treatment, we labeled the cells with Annexin V and PI fluorescein (Bender MedSystems, Austria) Cells were washed with 1×PBS, and suspended in 200 μl 1×binding buffer (10 mM HEPES pH 7.4, 140 mM NaCl,...
... cells invasiveness was suppressed in ER-negative cells MDA-MB-231 At the same time, the protein expression of MMP-9 was analyzed using western blotting The results showed that protein expression ... statistical significance of differences in mean values was assessed using Student s t test with SPSS 11.0 statistic software P < 0.05 was considered statistically significant Average values were ... metastatic and non-metastatic rat mammary adenocarcinoma cell lines[ 3,4] Overexpression of MTA1 plays an important role in tumorigenesis and tumor aggressiveness, especially tumor invasiveness and...
... understanding of their mode of action and why they are essential to life (7) Some studies have assessed the association between iron status and CAD risk but the results have been inconsistent ... use of nonspesific measures of body Fe stores, such as serum transferrin (16-19) The strongest supporting evidence that Fe is a risk factor for CAD stems from a cohort study of eastern Finnish ... Adsorption Spectrophotometer Statistical Analysis Data were presented as mean±standard deviation (SD) Using SPSS package 16.0 (SPSS Inc Chicago, IL, USA), data between the groups were compared with Student’s...
... further studies in certain regions of the world in age-related catarct cases with certain diets is suggested In this order, results can be compared with similar studies Conflict of interest The ... groups Discussion The most important result of this study is elevated serum Na+ level in those suffering from senile cataract which verifies previous studies [2, 25, 28] Though aging by itself is the ... control ones were mainly selected from the close relatives of the patients Both groups were investigated for any drug intake, hypertension and any other systemic disease Then positive ones of both...
... collection Statistical analyses Baseline characteristics of cases and controls were first compared Categorical variables were expressed as proportions, and compared using the c2 test Continuous variables ... a severity of disease classification system Crit Care Med 1985, 13:818-829 43 Soubassi LP, Chiras TC, Papadakis ED, Poulos GD, Chaniotis DI, Tsapakidis IP, Soubassi SP, Zerefos SN, Zerefos NS, ... demographics and clinical characteristics of the 20 subjects in this study are summarized in Table We studied 12 cases who developed at least Stage I AKI and control subjects who did not Cases and controls...
... SLE, systemic lupus erythematosus Statistical analysis Statistical analysis was performed using GraphPad Prism 3.03 (GraphPad Software, Inc., San Diego, CA, USA) and Statistica 7.1 (StatSoft, Inc., ... points No correlations between measured parameters were found in the patients with SLE Discussion This is the first study to demonstrate both an increase in BAFF levels in patients with SLE and ... http://arthritis-research.com/content/8/6/R167 Table Disease activity, B -cell status, BAFF, and APRIL at all time points in patients with SLE ID S4 S5 S7 S1 1 S1 3 S1 4 S1 6 S1 7 S1 9 S2 0 27/F 56/F...
... excess in hepatic copper in sheep in autumn is common in the inland districts of Troms and Trøndelag This is in line Acta vet scand vol 45 no 1-2, 2004 76 T Sivertsen & C Plassen with the results ... the south east inland districts, similar to the levels found in Trøndelag and northern Norway The present study includes 60 lambs from 10 flocks in south-east Norway Obviously, this is a small subset ... risk also in the south-east inland Recently, Strøm et al Cobalt and copper in lambs (2003) observed marginal serum levels of vitamin B12 in sheep from a farm in south-east Norway, indicating...
... compared using Student 's t-tests in pair wise comparisons between groups For comparisons within groups over time, consecutive recordings were compared with each other using paired t-tests Results The ... others, suggesting a lower resistance towards development PCV2-associated lesions of Landrace pigs [38,39] However, the station mixed pigs from different sources and also the effect of stressors and ... Development of rearing systems and health for fattening pigs in Sweden Sv Vet Tidn 2002, 54:469-474 Sorden SD: Update on porcine circovirus and postweaning multisystemic wasting syndrome Swine health...
... capable of inducing basophil degranulation and histamine release The presence of IgA containing-immune complexes in nasal secretions was not determined in this investigation If it is assumed that ... CIC and determine the serum levels of C3 and C4 in patients and to see if a correlation existed between these parameters and allergic rhinitis Methods Subjects and blood specimens One hundred and ... patients with rhinitis (65 females, 48 males, age range between and 82 years) were included in the study Criteria used to diagnose rhinitis included history, nasal obstruction, rhinorrhea and nasal...
... College of Chest Physicians/Society of Critical Care Medicine Consensus Conference: Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis Crit Care ... patients admitted with sepsis The implications of this pilot study are that septic medical ICU patients with AUD exhibit heightened stress response and less robust immune response in the setting ... incremental increase in cortisol in response to ACTH administration We defined cortisol as the baseline cortisol (prior to ACTH administration) Cortisols were tested in samples within one hour after...
... The skin shows a non-linear stress-strain relationship, behaves timedependent, incompressible, anisotropic and inhomogeneous, and is subjected to a prestress The complex structure of the skin ... white) Increasing the suction pressure led to an increasing uplift of the skin surface, an increasing fat thickness and a decreasing thickness of the skin composite At 19.4 kPa suction pressure ... important consideration inboth cosmetic and clinical applications such as the development of creams and personal care products, or in understanding skin diseases and skin ageing Especially knowledge...
... Interestingly, no changes was observed in CD4+CD8− T cells, resulting decreased CD4+/CD8+ ratio The summary of numbers was shown in Table Increased CD3+ cells, NK cells and MHC class II+ cells in ... observed in all groups Longer intake of LABs showed more effects as the all of wks groups showed above increase of 70 % (Fig 2) NK cells and MHC class II+ cells which are antigen presenting cells ... teratocarcinoma cells (1 × 106 cells/ mouse) were inoculated i.p at the day of 14th The change of body weights was measured at the intervals of days, and survival rate was assessed Statistics were done...
... evidence a synergistic effect of lutein and zeaxanthin in promoting growth and preventing stress; these results could be ascribed to the different setting of stress conditions used with respect to ... pigment-proteins from sucrose gradient bands (Figure 1A) was determined as previously described [9] The results are shown in Figure 2A The highest resistance was found in WT band 3, containing trimeric ... band 2, containing mostly minor Lhcbs, was more prone to photobleaching in agreement with previous findings [23] In the case of band from lut2.1, the resistance to photobleaching was, surprisingly,...
... installation suburbaine ou rurale sont, dans certains pays, s vốrement expos s aux surtensions Dans de tels cas, des tensions dessais plus ộlevộes, des essais de choc de foudre et dautres essais ... I S rie NOTE Dans certains pays, pour les transformateurs dont U,,, 72,5 k v , les essais CF sont prescrits comme des essais de s rie et les essais FI LD sont prescrits comme des essais de s rie ... impulse test stresses in other windings shall be limited to approximately 80 % of the assigned lightning impulse withstand voltages at these terminals Series windings in booster regulating transformers,...
... (a) and (b) show Kupffer cell staining, andin (d) portal vein endothelial VT1 cell staining Most VT1 staining is lost after CsA treatment but portal triad staining was retained 2070 FEBS Journal ... developed Both have focused on inhibitors of glucosyl ceramide synthase This enzyme is the first glycosyl transferase required for the synthesis of most GSLs, including Gb3 (in Fabry disease) and of ... biosynthesis in vivo was by no means assured The possibility of using MDR1 inhibition as a new approach to neutral GSL storage diseases is supported by our finding that CsA completely reverses GSL accumulation...
... which is already in clinical trials in cancer and Parkinson s disease at different stages Results AS101 reduced homocysteine-induced apoptosis of HL-60 cells The HL-60 cell line model system for ... hydrogens in homocysteine and its RP with AS101 As homocystine is composed of two homocysteine molecules, equivalent hydrogens inboth molecules possess similar magnetic resonance attributes, so the ... homocysteine overnight in vitro with or without AS101 was electrophoresed on a gel The gel was subsequently stained using silver staining The results showed that whereas homocysteine disassembled...
... during installation work • RF signals may affect improperly installed or inadequately shielded electronic systems in motor vehicles such as electronic fuel injection systems, electronic antiskid ... Vehicles RF signals may affect improperly installed or inadequately shielded electronic systems in motor vehicles such as electronic fuel injection systems, electronic antiskid (antilock) braking systems, ... the hands-free unit • Check the +12-V power pin and the ignition pin on the car radio and the respective pins of the ISO cable If necessary, swap the 21 Installation pins on the fuses of the ISO...
... implicated in diabetes [41] InS pombe, Ssp2 is the AMP-dependent protein kinase, and Ssp1 and Ssp2, whose mutants show similar phenotypes, interact closely [30,39] Schizosaccharomyces pombe responds ... [30] Ssp1 and Sds23 are functionally closely related as the phenotype of ssp1 kinase mutants was suppressed by the increased gene dosage of Sds23, and vice versa Glucose consumption was decreased ... glucose and 3.0–3.5 per 106 cells in mm glucose for or 12 h Similar results were obtained in cells in 1.1–4.4 mm glucose We also tested the loss of minichromosomes using the CN2 strain [27], and...