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fourier transform infrared microspectroscopy of complex biological systems from intact cells to whole organisms

nmr methods for the investigation of structure and transport

nmr methods for the investigation of structure and transport

Đại cương

... inverse Fourier transform (2.40) of the measured continuous Fourier transform (2.38) signifies To this end the discrete k values have to be expressed as a function of the index k and inserted into ... number of rows Noise Number of coil turns Number of discretization points in ˛ direction Number of averages Number of nuclear spins Set of indexes (first index) or number of slices Set of indexes ... dipole vector is replaced by the corresponding vector operator O and the energy by the Hamilton operator: O H0 D O B0 : (2.2) O The magnetic-dipole vector operator is proportional to the nuclear...
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Báo cáo y học:

Báo cáo y học: "Investigating the complementary value of discrete choice experiments for the evaluation of barriers and facilitators in implementation research: a questionnaire survey" pptx

Báo cáo khoa học

... tailored to the preferences of those concerned with the actual implementation The objective of our study was to investigate the complementary value of DCE for the evaluation of barriers and facilitators ... it possible to gain insight into the relative importance of barriers to the implementation of change, and the trade-offs people make between barriers on the one hand and facilitators on the other ... supportive caregiver towards informal caregiver, from clinician to nurse specialist, from ward nursing staff to outpatient nursing staff, and from hospital-based nursing staff to home care nursing...
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báo cáo khoa học:

báo cáo khoa học: " Investigating the complementary value of discrete choice experiments for the evaluation of barriers and facilitators in implementation research: a questionnaire survey" pot

Báo cáo khoa học

... tailored to the preferences of those concerned with the actual implementation The objective of our study was to investigate the complementary value of DCE for the evaluation of barriers and facilitators ... it possible to gain insight into the relative importance of barriers to the implementation of change, and the trade-offs people make between barriers on the one hand and facilitators on the other ... supportive caregiver towards informal caregiver, from clinician to nurse specialist, from ward nursing staff to outpatient nursing staff, and from hospital-based nursing staff to home care nursing...
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Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

Báo cáo khoa học

... derived from ambiguous NOEs that are not uniquely assigned to a single pair of protons at the end of the last cycle are automatically purged from the output list [78] The software-generated list of ... 39–81 of VpU The dotted lines mark the theoretical average values of the downfield shift of 13Ca (top) and 13CO (bottom) as well as of the upfield shift of 1Ha resonances (middle) characteristic of ... number of cross-peaks of VpUcyt were obtained with perdeuterated DPC (DPC-d38) at 30 °C A series of HSQC spectra of mm VpUcyt was recorded with varying amounts of DPC-d38 in the sample (from to 200...
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Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc

Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc

Báo cáo khoa học

... using COFRADIC, we select subsets of peptides related to the biological question under investigation For instance, for the study of the oxidation of protein methionines during oxidative stress, cells ... dephosphorylation of phosphopeptides [37]), or by converting it into a moiety with altered properties (e.g by reducing nitrotyrosine to aminotyrosine [38]) An overview of the COFRADIC sorting protocols ... COFRADIC and protein modifications analysis of purine-binding proteins in a total lysate of human Jurkat T -cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing ) protease degradomics...
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Tài liệu Mesoscopic Model for Mechanical Characterization of Biological Protein Materials docx

Tài liệu Mesoscopic Model for Mechanical Characterization of Biological Protein Materials docx

Kĩ thuật Viễn thông

... comparable to mechanical properties of biological protein materials obtained from experiments and/or atomistic simulations Further, we discuss the role of native topology on the mechanical properties of ... N is the total number of atoms for a protein crystal in a unit cell, rij = rj – ri with the position vector of ri for an atom i in a unit cell, Φ(rij) the inter-atomic potential for atoms i and ... such as atomistic model (e.g molecular dynamics simulation) for protein crystal Despite of the ability of atomistic model to provide the macroscopic properties of protein crystals,28 the atomistic...
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Báo cáo khoa học: Affinity purification-mass spectrometry Powerful tools for the characterization of protein complexes ppt

Báo cáo khoa học: Affinity purification-mass spectrometry Powerful tools for the characterization of protein complexes ppt

Báo cáo khoa học

... assembly of a protein complex In addition, overexpression can occasionally cause cytotoxicity This might be overcome by use of sophisticated vector systems in which the expression level of the epitope ... retrieval of protein complexes from endogenous components of cells and tissues which is obviously closest to resembling physiological conditions as there is no need for ectopic expression of the ... downside of epitope-tagging is obviously the need to ectopically express proteins in cells which largely limits this approach to cell culture systems Tight control over the expression level of bait...
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Báo cáo sinh học:

Báo cáo sinh học: " Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" pptx

Hóa học - Dầu khí

... transition (EMT), a process that has been linked to the generation of cells with properties of stem cells, and to the ability of tumor cells to enter the circulation and seed metastases EpCAM-CK ... samples, found to be negative for presence of EpCAM+CK+CD45- cells, EpCAM-CK +CD45- cells were detected in 38% (14/37) of peripheral blood samples, and the median of the number of these cells was ... fraction resulted to be lower in comparison to the mean fluorescence of the SW620 cells and considerably lower in comparison to the fluorescence of the SW620 cells we were able to detect in the...
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báo cáo hóa học:

báo cáo hóa học:" Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" docx

Hóa học - Dầu khí

... transition (EMT), a process that has been linked to the generation of cells with properties of stem cells, and to the ability of tumor cells to enter the circulation and seed metastases EpCAM-CK ... samples, found to be negative for presence of EpCAM+CK+CD45- cells, EpCAM-CK +CD45- cells were detected in 38% (14/37) of peripheral blood samples, and the median of the number of these cells was ... fraction resulted to be lower in comparison to the mean fluorescence of the SW620 cells and considerably lower in comparison to the fluorescence of the SW620 cells we were able to detect in the...
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báo cáo khoa học:

báo cáo khoa học: "Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay" docx

Báo cáo khoa học

... purchased from Sigma Aldrich Coumarin 521 was purchased from Exciton (Dayton, Ohio, USA) Micro-centrifuge tubes (50 kDa and 100 kDa MWCO) were purchased from Fisher Scientific Preparation of buffer ... immediately after preparation Stock solutions of 2-mercaptoethanol (1 M) and hydroxylamine hydrochloride (1 M) were prepared and stored at room temperature Stock solutions of cysteamineHCl (100 mM) ... with the addition of 2-mercaptoethanol (1 M) to a final concentration of approximately 20 mM and the mixture was left to stand for 10 minutes The activated QDs were purified of unreacted reagents...
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báo cáo khoa học:

báo cáo khoa học: " A new model for the characterization of infection risk in gunshot injuries:Technology, principal consideration and clinical implementation" pptx

Báo cáo khoa học

... carried out to identify the contamination of gunshot injuries focused on providing quantitative proof of the existence of bacteria It was impossible to determine the relationship to the bullet ... to differences in the depths to which they penetrate into the gelatin block These can be attributed to the fragmentation behavior of the soft point bullets and the associated higher release of ... contrast to this, the infiltration depth of barium titanate particles in the case of the full metal jacket projectile was significantly lower in the area 8.0 cm from the entry up to the exit of the...
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Báo cáo y học:

Báo cáo y học: "Novel multiplex technology for diagnostic characterization of rheumatoid arthritis" pdf

Báo cáo khoa học

... conversion of the lowest 5% of signals to black and the top 5% of signals to white, with the color scale adjusted accordingly The rheumatoid arthritis sample analyzed exhibits very high levels of autoantibody ... rheumatoid arthritis; RF: rheumatoid factor; SAM: significance analysis of microarrays; SE: shared epitope; TNF: tumor necrosis factor Acknowledgements We thank members of the Robinson Laboratory ... levels of osteoprotegerin and soluble receptor activator of nuclear factor kappa B ligand in serum of rheumatoid arthritis patients and their normalization after anti-tumor necrosis factor alpha...
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IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE

IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE

Y khoa - Dược

... Various non– cancerous cells, such as hematopoietic progenitor cells, express relatively high levels of ALDH1A1 and hence are relatively resistant to oxazaphosphorine–induced toxicity (Sladek et ... cessful isolation of viable hematopoietic stem cells from human umbilical cord blood and was reported to be specific for ALDH1A1 (Storms et al., 1999) However, while ALDH isoforms show substrate ... reported to be associated with hepatotoxicity in alcoholics, late onset of Alzheimer’s and Parkinson’s disease Hepatotoxicity in alcoholics occurs due to competition of lipid peroxidation product–derived...
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guide for structural maintenance of parking structures

guide for structural maintenance of parking structures

Cơ sở dữ liệu

... tends to create conditions conducive to progressive deterioration of the structural concrete Spalling on the top surfaces of the deck can lead to rapid deterioration due to the ponding of water ... Stair and elevator towers—Leaks often occur through joints between the deck slab and stair and elevator towers This problem can often be attributed to poor drainage around the towers Drainage ... mechanism of joint sealants is deterioration of the surfaces to which the sealant is bonded, allowing the intrusion of water and subsequent progressive failure of the sealant along the length of the...
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Application of biased scanning probe microscopy techniques for multifunctional characterization of bifeo3 and zno thin films

Application of biased scanning probe microscopy techniques for multifunctional characterization of bifeo3 and zno thin films

Cao đẳng - Đại học

... FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MECHANICAL ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2011 Preface This dissertation is submitted for the degree of Doctor of Philosophy ... in the Department of Mechanical Engineering, National University of Singapore (NUS) under the supervision of Associate Professor Zeng Kaiyang To the best of my knowledge, all of the results presented ... like to thanks Prof John Wang and Prof Ding Jun at Department of Material Science and Engineering NUS, for their valuable discussion during the collaboration of the research works I am thankful to...
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Structural characterization of nogo proteins  implications for biological functions and moleculedesign of therapeutic applications

Structural characterization of nogo proteins implications for biological functions and moleculedesign of therapeutic applications

Cao đẳng - Đại học

... folds back to the convex side of the LRRs of NgR1 (Wen et al., 2005) Because NgR1 lacks a cytosolic component, it would appear to require a transmembrane co-receptor to transduce an inhibitory signal ... formation of endothelial cells in vitro The identification of NgBR facilitate 20 understanding the function of NogoB as a regulator of vascular remolding and may also lead to the discovery of agonists ... turned out to be clinically useless due to its effect of increasing sensitivity to pain (Schwab, 2002) 1.1.2.3 Inhibitory molecules in the CNS The lack of CNS regeneration was not only due to the...
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Tài liệu ComProtect® Solid-State Single-Pair Protectors for Series 2 Switching Blocks docx

Tài liệu ComProtect® Solid-State Single-Pair Protectors for Series 2 Switching Blocks docx

Phần cứng

... 400V 20 sec sec 50 MΩ 1000Vrms  dB up to 10 MHz 0.2 dB up to 10 – 14 MHz 23 dB up to MHz 32 dB up to 100 MHz -40° to 85° C (-40° F to 185 ° F) UL94 V-0 ±10A, 10/1000µs 1500 operations ... transport and storage • NEBS Level compliant • Insertion loss of less than 0.6 dB (up to 16 MHz) • The ComProtect module supports broadband circuits, such as ADSL2+ Applications: •  Fiber to the curb ... Package of 8, black housing 5909 211-00 ComProtect® protectors without ground bars Package of 25 Black housing 6659 008-30 Yellow housing 6659 008-90 Red housing 6659 008-50 Package of 100 Black...
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Fundamentals of quantum mechanics for solid state electronics, optics   c  tang

Fundamentals of quantum mechanics for solid state electronics, optics c tang

Vật lý

... for example, instead of a billiard ball, a tiny atomic or subatomic particle In the process of scattering at least one photon from the particle to a photodetector in order to measure its position, ... is the Hamiltonian of the system The Hamiltonian is the operator corresponding to the total energy of the system From Postulate 2, it is always possible to write down such an operator for any ... range from x1 to x1 + dx to that in the range from x2 to x2 + dx is in the ratio of Nx1: Nx2 ¼ jYðx1 Þj2 : jYðx2 Þj2 The expectation value of the position of the particle, hxiY , is the average of...
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nanomaterials for solid state hydrogen storage, 2009, p.346

nanomaterials for solid state hydrogen storage, 2009, p.346

Vật lý

... alternative energy vector It is ubiquitous, clean, efficient, and can be produced directly from sunlight and water by biological organisms and using semiconductor-based systems similar to photovoltaics ... Synchronic History of Development of Hydrides and Nanomaterials Early Investigations of Metal–Hydrogen Systems and Hydrides Although not considered to be stored and delivered from metal-hydrogen systems, ... ball travels from the o’clock position, at the bottom of the cylinder, to the o’clock position, and then falls to the bottom of the reactor The point where each ball is detached from the wall...
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