... identifying patients at risk for complicated diverticular disease. Dis Colon Rectum. 1991;34:339-42. 4. Kourtesis GJ, Williams RA, Wilson SE. Surgical options in acute diverticulitis: value of sigmoid ... Schumpelick V. Increased distribution of collagen type III and reduced expression of matrix metalloproteinase 1 in patients with diverticular disease. Int J Colorectal Dis. 2001;16:271-5. 3. ... (arrow). Discussion The prevalence of diverticulum in patients aged 65 years or more is as high as 65%, but merely 5% in those below 40 years of age [6]. Sigmoid colon diverticulum is closely...
... connectivity company is introducing "Patch by Exception", the next generation in cable management systems.Patch by Exception guarantees network managers a system that is not only flexible, ... even easier.HOW TO GET THE MOST OUT OF PATCH BY EXCEPTIONMany organisations have successfully implemented the Patch by Exception solution by establishing a maintenance contract with an installer ... changes without the need for assistance from an installer.To date this has been achieved by assigning a hub port and an extension port to each individual within an organisation. If an individual...
... the tissuesproducing it. In response to tissue injury, such ashepatic and renal injury, the inactive single-chain HGF is converted to a two-chain form exclusively inthe injured tissue. This ... exclusively inthe injured tissue. This conversion is mediated by ser-ine protease activity, which is induced in the injuredtissue [28]. The two-chain form is required for the bio-logical activity ... TMPRSS13.Proteolytic activation of pro-HGF by TMPRSS13Pro-HGF is proteolytically activated by matriptaseand hepsin, and the protease activity is inhibited by HAI-1 [9,10,18]. Therefore, we examined...
... investigations that includeanalysis of pancreatic islet cells.The H-PGDS TG mouse is a novel obesity modelwith which to investigate the mechanism of adipogene-sis. As is the case for obese people ... et al. (2001) The mechanisms by which bothheterozygous peroxisome proliferator-activated receptorgamma (PPARgamma) deficiency and PPARgammaagonist improve insulin resistance. J Biol Chem 276,41245–41254.38 ... molecular mecha-nisms underlying adipogenesis may lead to the devel-opment of strategies for reducing the prevalence ofobesity.Adipogenesis is a complex process accompanied by various changes...
... followed by Strep-tag IIwas isolated by BamHI digestion and inserted into theBamHI site of pmGFP–calpain 7.To construct pCMV3xFLAG–IST1 and pGEX–IST1, anIST1 cDNA fragment was amplified by PCR, ... autolysis. Therefore, it is prematureto draw conclusions regarding the mechanism of theenhancing effects of IST1 on mGFP–calpain 7–Strepautolysis in vitro.Both mGFP–calpain 7 and IST1 have ... FLAG–IST1 did notenhance the autolysis of mGFP–calpain 7 in culturedcells. On the other hand, overexpression of FLAG–IST1DMIM1,2suppressed the autolysis. This observationmight be explained by...
... Kmvalues is exclu-sively displayed by the tetramer forms, and low-efficiency kinetics with high Kmvalues is displayed by the dimer forms. These observations strengthen theprevious hypothesis that ... Km is the Michaelisconstant and K0.5 is the substrate concentration wherev = 0.5 Vmax. When n = 1, there is no co-operativity, andK0.5= Km.AcknowledgementsThis work was supported by ... behaviour after dilution to 6 lgÆmL)1indicate thatdissociation of the tetramer to the dimer is a slowlyprogressing process. This is supported by the kineticbehaviour of the recombinant enzyme...
... wasdetected using MUPP1 antibodies.ImmunohistochemistryMice were raised and maintained according to governmen-tal and institutional care instructions. Immunohistochemis-try was carried out on 14 lm ... different odorant molecules is governed by the diverse array ofolfactory receptors expressed by olfactory sensory neurons in the nasalepithelium. Olfactory receptors consist of seven transmembrane ... betweenMUPP1 and OR2AG1 is inhibited by over-expressingthe interacting PDZ domains, the resulting response ofOR2AG1 to odorant is modified in that the rapiddecay of signal is impaired.We finally...
... cancer. The activity of cyclin dependentkinase 4 is controlled by multiple mechanisms, including phosphorylationof tyrosine 17. This site is equivalent to tyrosine 15 of cyclin dependentkinase ... in HeLa lysates. C-YES is a 62 kDa nonreceptor tyrosine kinase of the Srcfamily that is expressed in a wide range of tissues [24].The N-terminus of C-YES is dually myristoylated andpalmitoylated ... thyroid epithelial cells: activation by TSH, inhi-bition by TGFbeta, and phosphorylations of itssubunits demonstrated by two-dimensional gel electro-phoresis. Exp Cell Res 291, 135–149.35 Sexl...
... aureus is a major food- borne pathogenwhich produces a number of toxins and virulence fac-tors [1]. The staphylococcal enterotoxins produced by S. aureus are known to cause staphylococcal food poisoning, ... cytokine synthesisand febrile response induced by SEC1 is dependent onNADPH oxidase and not on PI3K ⁄ Akt or 5-LOX ⁄FLAP because these phenomena are attenuated by apocynin and not by ETYA, MK ... andIL-6 that is blocked by the NF-jB inhibitor (PDTC orSN-50). Moreover, the NF-jB binding ability is abro-gated by binding site competition and antibody super-shift analysis. These findings...
... precipitated DNA was analyzed by 1% agarose gel electro-phoresis. DNA was visualized by staining with ethidium bromide. Results are represented by negative images. Asterisks indicate nucleicacids ... and were isolated as a single peak fraction by a 0.1–1.0 m NaCl linear gradient in R buffer. Histone H1,which exists in the DNA affinity fraction, was separated asa major component stepwise elution ... nuclear focilinked to phosphorylated histone H2AX (c-H2AX) [3],which is responsible for the redistribution of repairfactors to DSB sites [4], although it is dispensablefor initial damage recognition...
... multigenefamily is modulated by distal cis elements. J Biol Chem271, 28146–28153.24 Sharma S & Gopinathan KP (1996) Role of TATATAAelement in the regulation of tRNAGly1gene expression inBombyx ... examined by including heparin (20 lgÆmL)1) in the binding reaction (lanes 5,6, both panels). The complex formation was analysed by electrophoresis on 4% polyacylamide (nondenaturing) gels and visualized ... as marked. The heparin-resistant complex on tRNAGly1-1 (left) is marked by an arrow; ++ denotes 6 lg of protein. (B) Thespecificity of complex formation was examined by the competition with...
... this gene is controlled by Ets-1 and requires thepresence both of the Sp1 and Sp3 transcription factors. Thepresence of this complex multicomponent PRD in the fgl2proximal promoter is consistent ... pre-sented in this study suggest that this PRD is necessary forthe inducible expression of fgl2, we plan to undertakeadditional studies aimed at more precisely elucidatingspecific mechanisms regulating ... [2,3], glomerulonephritis [4,5], septic shock [6,7],and bacterial and viral infections [8]. For example, tissuefactor (TF) [9], the transmembrane receptor of factor VII, is the major procoagulant...
... SODs is not governed by one or even two residues, but is most likely accomplished by the concerted effects of a combination of key residues.Further analysis of these and other mutant SODs is currently ... electrophoresis (PAGE)Native 8% polyacrylamide gels (acrylamide : N,N¢-methyl-enebisacrylamide, 29 : 1, w/w) containing NaCl/Tris,pH 8.8 utilized a Tris/glycine electrophoresis buffer systemconsisting ... N-terminal aminoacid residues. SOD is released from GST-SOD fusionproteins by cleavage with FXa (Fig. 2C). Although thismethod is less efficient than cleavage by thrombin [24] thereleased SOD...
... semiquantitatively by t his method. The effects ofenzymes, inhibitors and other reagents on the cell cycle–dependent interaction could also be examined very easily by means of t his method. This method is ... meiotic division until they meet sperm. In thatphase, the nuclear envelope is dispersed in the cytop lasm asnuclear envelope precursor vesicles. Cell cycle progression is triggered by fertilization, ... shown by sperm chromatinin vitro ([6,13]; this study), and by mitotic phase chromo-somes f rom C HO cells [5]. Regulation of the binding ofLBR to chromatin by phosphorylation was shown in thisstudy...