fluorescence in situ hybridization for cancer related studies

... Traditional Chinese Medicine Hospital which are the two main hospitals for the treatment of breast cancer in Guilin city of China The specimens were fixed in 10% neutral-buffered formalin (pH7.4) for 24 ... Isola J: Chromogenic in situ hybridization: a practical alternative for fluorescence in situ hybridization to detect HER-2/ neu oncogene amplification in archival breast cancer samples Am J Pathol ... Evaluation of clinical outcomes according to HER2 detection by fluorescence in situ hybridization in women with metastatic breast cancer treated with trastuzumab Clin Breast Cancer 2005, 6(3):240-6...

Ngày tải lên: 09/08/2014, 04:21

... 2.5: Fluorescence in- situ hybridization: Fluorescence in- situ hybridization (FISH) is a technology utilizing fluorescently labeled DNA probes (with a fluorescent dye or a hapten, usually in the form ... anomalies in standard karyotyping for prenatal and pre-implantation genetic diagnosis and cancer cytogenetics 2.5.5: Fluorescence in- situ hybridization (FISH) as a sensitive tool in detecting mosaicism: ... FISH: Fluorescence in situ hybridization (FISH) is a rapid reliable technique in molecular cytogenetics It supplements conventional karyotyping and in some cases provides additional information...

Ngày tải lên: 04/10/2015, 15:45

... without a cancer diagnosis were stained Multiple 4-μm sections were cut with a Micron microtome (HM355S) and used for in situ hybridization analysis In Situ Hybridization (ISH) In situ hybridization ... in formalin-fixed tissue, making them ideal candidates for investigation in routinely processed material Among the miRNAs, miR-155 is particularly interesting as it is consistently involved in ... validation studies are needed to confirm these results Potential microRNA-based therapy is now being exploited in cancer, attempting to modulate their expression, reintroducing microRNAs lost in cancer, ...

Ngày tải lên: 18/06/2014, 16:20

... Okjin Kim Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by in situ hybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated ... resulted in the increasing sensitivity as compared with hourshybridization protocol Digoxigenin-labeled probe was capable of detecting CHV in MDCK cells infected with 103 TCID50 using overnight hybridization ... enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled probe,...

Ngày tải lên: 07/08/2014, 17:22

... DNA in bovine erythrocytes by slot-blot and in situ hybridization with a PCRmediated digoxigenin-labeled DNA probe J.Vet Diagn Invest 7, 465–472 [47] Gebhart, C.J., 1994 Specific in situ hybridization ... necessary for colonization of large intestines of streptomycin-treated mice Infect Immun 67, 6152–6156 [37] Felske, A., 1998 In situ detection of an uncultured predominant Bacillus in dutch grassland ... Boye, M., 1998 Specific detection of the genus Serpulina, S hyodysenteriae and S pilosicoli in porcine intestines by fluorescent rRNA in situ hybridization Mol Cell Probes 12, 323–330 [20] Brown-Howland,...

Ngày tải lên: 30/10/2012, 11:02

... by inversion continually for the first 30 s then two inversions every 30 s Rinse twice with tap water Fix for min, with agitation, with Ilford Rapid Fixer (Subheading 3.5.5.) Rinse in running ... (1988) Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries Hum Genet 80, 224–234 Collins, C., Lin Kuo, ... Engineering Council of Canada and by SeeDNA Biotech Inc References Lawrence, B J (1990) A fluorescence in situ hybridization approach for gene mapping and the study of nuclear organization, in...

Ngày tải lên: 11/04/2014, 09:47

... We have called this a double in situ approach, because the in situ synthesis of the nanoparticle (TiO2) coincides with the in situ polymerization, resulting in the formation of a well-dispersed ... according to ASTM D2863-97 Vertical burning tests (UL-94) were conducted on a vertical burning test instrument (CZF-2-type) (Jiangning, China) with sheet dimensions of 130 13 mm3 according to ... spread but the presence of flaming drips, while V-0 shows self-extinguishing behaviour without burning drips While the increase in melt viscosity is to be expected on incorporation of well-dispersed...

Ngày tải lên: 22/06/2014, 01:20

Ngày tải lên: 28/06/2014, 15:23

... and Yukinori Yoshimura, 52001 A sensitive non – radioactive in situ hybridization method for detection of chicken IgG - chain mRNA: a technique suitable for detecting of variety of mRNA in tissue ... Biotinylated probes for in situ hybridization histochemistry: use for mRNA detection J Histochem Cytochem 41: 1751 – 1754 22 Heitzman H., Richards F.M., 1974 Use of avidin – biotin complex for ... Procedures Online 3: – Japan 18 Baumgart E., Schad A., Grabenbauer M., 2001 In situ hybridization: general principles and application digoxigenin – labeled cRNA for detection of mRNAs In Immunocytochemistry...

Ngày tải lên: 28/07/2014, 02:21

... tuyệt đối, xylene chất thay xylene, nƣớc cất hai lần, chloroform, paraffin, formalin, formaldehyde 37%, acid acetic nguyên chất, paraformaldehyde dạng bột, Bismark Brown Y dạng bột, keo dán (Boume ... 12,5 Paraffin đƣợc dùng quy trình xử lý mẫu hỗn hợp với sáp ong theo tỷ lệ paraffin : sáp ong III.5.2.3 Đúc mẫu paraffin Đặt mẫu xử lý vào khuôn inox, dùng bình rót paraffin (5 paraffin : sáp ong) ... dịch Blocking buffer 1X, chất blocking dung dịch khóa phân tử lai không đặc hiệu lại  Ủ mẫu với dung dịch Anti – Digoxigenin (Anti – DIG) có gắn Alkaline phosphatase (AP), Anti – Digoxigenin kết...

Ngày tải lên: 28/07/2014, 02:21

... phƣơng pháp phòng bệnh virus đốm trắng II.5 PHƢƠNG PHÁP IN SITU HYBRIDIZATION II.5.1 Sơ lƣợc phƣơng pháp In situ hybridization Phƣơng pháp In Situ hybridization (ISH) đƣợc phát vào năm 1969 công Gall ... : In Situ hybridization PCR : Polymerase Chain Reaction ELISA : Enzyme Linked Immuno Sorbent Assay RT – PCR : Reverse – transcription polymerase chain reaction PBS : Phosphate Buffered Saline ... 14 II.4.2.5 Phòng trị bệnh 14 II.5 PHƢƠNG PHÁP IN SITU HYBRIDIZATION 14 II.5.1 Sơ lƣợc phƣơng pháp In situ hybridization 14 II.5.2 Khái niệm lai phân tử ...

Ngày tải lên: 28/07/2014, 02:21

... results also showed that dark staining was observed around the nucleus predominately, indicating that β-actin mRNAs existed mainly in the vicinity of the nucleus In order to analyze the correlation ... following previous studies [12,13] ISH for β-actin mRNA of single cells Digoxigenin (DIG) labeled RNA probe preparation β-actin cDNA [224–987 bp] was prepared by RT-PCR βactin cDNA was inserted into ... (a-c) Each square indicates the region of the AFM probe insertion Numbers in lower panels indicate β-actin mRNA quantities detected by the SCN method, and dark intracellular staining indicates distribution...

Ngày tải lên: 11/08/2014, 00:22

... used for in situ, hybridization has been described elswhere (Gellin et al., 1985), and we added only some modifications concerning the washings after hybridization: the slides were first rinsed in ... explain the efficiency of in situ hybridization This observation confirms the one given by Lin et al 1985 We have also modified the washings after hybridization They are less stringent and, in ... & Labonnardi6re C (1986) Molecular cloning and sequencing of a gene incoding biologically active porcine interferon alpha J Interferon Res 6, 349-360 Lin C.C., Draper P.N & De Braekeleer M (1985)...

Ngày tải lên: 14/08/2014, 19:23

Ngày tải lên: 19/12/2014, 20:12

... stiffness index UK – United Kingdom YB – Yolande Borthwick Chapter Introduction Breast cancer is the most common cancer affecting women in Scotland, accounting for 28% of all cancers in the Scottish ... (http://globocan.iarc.fr/factsheets/cancers/breast.asp) Global incidence of breast cancer continues to increase (WHO 2008) and breast cancer survival rates are reported as varying between 40% in low income countries and 80% in ... which will result in oedema formation Chronic venous insufficiency results in damage to the vessels in the vascular system resulting in increased pressure and increased production of interstitial...

Ngày tải lên: 22/12/2014, 21:42

... industry include : • decreasing feature size • increasing need for reduced defect density • increasing interconnect levels • reducing chip cost These trends place tremendous pressure on the industry ... the spin-coating and baking performed within the same machine, latter processing steps can commence as the former nears completion For instance, towards the end of the spin-coating step with ... inspection Surface inspection for etch irregularities and other problems Table 2-1 Summary of steps for fabricating a single layer In general, the sequence of steps for patterning a single layer can...

Ngày tải lên: 04/10/2015, 15:53

... care provider trained in screening for skin cancers The general population not at increased risk of skin cancer There is at this time no evidence for or against skin cancer screening of the general ... of the skin be offered surveillance by a physician, including total-body skin examination and counselling to perform skin self-examination? What characteristics should clinicians assess in order ... recommended for individuals at average or low risk for skin cancer Key Evidence (Please see Section for the complete systematic review of the evidence conducted by the Skin Cancer Screening Guideline...

Ngày tải lên: 15/02/2014, 05:20

... a basis for selecting a “suite of indicators” to measure disparities in screening, risk factors, and other cancer- related health objectives Initiatives to Eliminate Health Disparities In 1979, ... healthy)? In regard to reducing income disparities, we are comfortable as a society in considering the need to reduce the incomes of the advantaged via taxation in order to increase the incomes ... information about the sources of increasing or decreasing disparity as indicated in a summary statistic In public health, decomposition often is used to capture differences in summary rates For...

Ngày tải lên: 06/03/2014, 01:20

... diagnosis of cancer in 2002 In spite of the scale of the cancer burden in Ireland, there have been improvements both in curtailing the risk of developing cancer and in increasing cancer survival in recent ... evolving cancer control system stimulating high-quality research on all aspects of cancer control developing and maintaining international alliances in support of cancer control A Strategy for Cancer ... continue to divide and grow to produce tumours There are several main types of cancer Carcinoma is cancer that begins in the skin or in tissues that line or cover internal organs Sarcoma is cancer...

Ngày tải lên: 15/03/2014, 00:20

... detailed instructions from the investigator before leaving the testing room on how to insert the probe Once inserted, they were encouraged to relax on a reclining chair for 10 minutes before watching ... used a process of discussing discrepancies and resolving them as a team in line with the guidelines for analysis we developed for each theme Psychophysiological Recording Vaginal pulse amplitude ... Psychosexual functioning after treatment for gynecological cancer: An integrative model, review of determinant factors and clinical guidelines International Journal of Gynecologic Cancer, 2, 281-290...

Ngày tải lên: 22/03/2014, 10:20