excitation contraction coupling in human embryonic stem cell derived cardiomyocytes
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... lncRNAs involved in differentiation has been carried out in human cells In this thesis, functional characterization of lncRNAs in human development is addressed using human embryonic stem cells ... exhibiting trans-acting transcriptional cooperativity with DLX homeodomain proteins and increasing Dlx5/6 enhancer activity in a neural stem cell line (Feng et al., 2006) In the developing ventral ... to induce pluripotency in adult somatic cells (Takahashi and Yamanaka, 2006), further demonstrating the pivotal role of transcription factors in the maintaining and inducing the pluripotent cell...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
... target 226 lincRNAs expressed in mESCs, and the authors identified 26 lincRNAs that resulted in loss of Nanog and Oct4 expression upon lincRNA knockdown, indicating that lincRNAs maintain the pluripotent ... governing stem cell pluripotency and cell fate determination, and perturbations of lncRNA levels will result in stem cell differentiation 104 Chapter VII – Long Non-coding RNAs are Indispensable in ... lncRNAs in the maintenance of hESC pluripotency, and their involvement in neuronal differentiation respectively 82 Chapter VI – Long Non-coding RNAs Regulate Human Embryonic Stem Cell Pluripotency...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
... expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST chromatin-immunoprecipitation ... database indicates the presence of a REST binding site upstream of RMST REST (or NRSF) binding sites were observed upstream of RMST in three human cell lines, namely GM12878, a lymphoblastoid cell line, ... chromatin binding Quantitative PCR of the ChIP DNA revealed REST occupancy at the region upstream of RMST (pRMST), confirming that REST binds upstream of RMST in the human neural stem cell line...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
... very few human lncRNAs have been studied with such extent In this thesis, I focused on examining lncRNAs involved in the pluripotency maintenance of human embryonic stem cells (hESCs), and human ... Liu, Y., Shin, S., Zhan, M., Rao, M.S., and Zeng, X (2010) Stage-specific role for shh in dopaminergic differentiation of human embryonic stem cells induced by stromal cells Stem Cells Dev 19, ... and Freed, W.J (2008) Assessment of stromal -derived inducing activity in the generation of dopaminergic neurons from human embryonic stem cells Stem Cells 26, 1517-1525 Walker, E., Chang, W.Y.,...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 1
... anchoring substrates are the underlying principles of these systems, which try to mimic the microenvironments of the hESCs Indeed, keeping in mind the origin of hESCs, the neighbouring cells ... generating new epithelium and basement membrane, providing anchorage for the hESCs [18] The presence of ECM proteins in developing embryos indicates that these proteins play a role in maintaining ... Crook of Singapore Stem Cell Bank, Singapore Stem Cell Consortium, Institute of Medical Biology The iPSCs were derived by transfecting IMR-90 fetal fibroblasts with lentiviral vector expressing Oct-4,...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 2
... 105340 Keratin, type I cytoskeletal 10 Keratin, type II cytoskeletal Collagen alpha-1(I) chain*† Collagen alpha-2(I) chain*† EMILIN-1*† Fibrillin-1* Isoform of Collagen alpha-1(XII) chain Isoform ... metalloproteinase with thrombospondin motifs 1*† Transforming growth factor-beta-induced protein ig-h3 74665 122841 234698 Isoform of Extended synaptotagmin-1*† Isoform of Myoferlin*† 99.80% ... alpha-2(VI) chain Thrombospondin-1* Vitronectin* 100.00% 12.40% Isoform of Tenascin*† Isoform of Transglutaminase 100.00% 10.50% 99.80% 4.61% 100.00% 99.80% 8.29% 8.79% Thrombospondin-1* 100.00%...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 3
... aid in increasing integrin binding and hence cell binding This contradiction could possibly be due to the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering ... maintain hESCs in a pluripotent state This observation is surprising, since these matrices contains more Fn (by immunofluoresence comparison, section 3.3.2), it would be expected that the 52 increased ... interfering with TGFB signaling for the hESCs, since DCN and BGN both bind active TGFB, and could lead to a decrease in the amount of active TGFB available for the hESCs 3.5 Matrices that maintain...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 4
... under-expression of Oct-4 in ES cells leads to their differentiation into trophoectoderm lineages [10], indicating the crucial role that Oct-4 plays in maintaining the pluripotent state of ES cells As Oct-4 ... maintained in the embryonic ectoderm at the cylinder stage, and down-regulated at gastrulation in an anterior-posterior pattern Germ cells continue to maintain the expression of Oct-4 until the initiation ... within the nuclei 64 of hESCs, and so, in order to obtain successful Oct-4 immunostaining, the cells have to be first permeabilized using 0.2% Triton-X 100 - this extra processing step results in...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 5
... In vitro induced differentiation hESCs cultured on DxSDOCDOC for 13 passages were induced into neural differentiation using a protocol modified from WiCell Research Institute, National Stem Cell ... will still prove superior at increasing the population doublings and also in 84 maintaining the pluripotent characteristics of the hESCs during their propagation Once again, hESCs were cultured onto ... Stem Cell Bank In this protocol, the hESCs formed embryoid bodies using non-adherent cell culture plates and were transferred onto laminincoated plates and incubated in neural induction medium...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 6
... possibility of developing an alternative embryonic stem cell culture system, with the aim of overcoming current obstacles that hinder the safe clinical implementation of human embryonic stem cell culture ... signatures of seven individual uman embryonic stem cell lines Stem Cells 23:1343-1356 (2005) Stewart R, Stojkovic M, Lako M Mechanisms of self-renewal in human embryonic stem cells Eur J Cancer ... Viiri KM, Kaukinen K, Maki M, Lindfors K Extracellular transglutaminase has a role in cell adhesion, whereas intracellular transglutaminase is involved in regulation of endothelial cell proliferation...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
... Glial cell- derived nerve factor Glial fibrillary acidic protein Glial progenitor cell Homeodomain Human embryonic stem cell High mobility group Herpes simplex virus thymidine kinase Interferon Interleukin ... feeder-free conditions according to the technical manual “Maintenance of Human Embryonic Stem Cells in mTeSRTM1” (STEMCELL Technologies Inc.) In brief, H1 hESCs were maintained on BD matrigel hESC-qualified ... domains, which are highly conserved DNA-binding domains In recent years, Sox2 has become widely regarded as a reprogramming factor important for inducing and maintaining 15 pluripotency in ESCs...
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Báo cáo y học: "Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes" doc
... the cardiomyocytes derived from embryonic stem (ES) cells Mouse ES cells can proliferate indefinitely without senescence in vitro in their undifferentiated state in the presence of leukemia inhibitory ... gene interactions AP recordings were performed in single α-MHC cardiomyocytes and in multicellular α-MHC+ cell aggregates at days 11 to 16 Single cardiomyocytes were obtained by dissociating puromycin-purified ... Hescheler J, Sachinidis A: Embryonic stem cells: a promising tool for cell replacement therapy J Cell Mol Med 2004, 8:465-473 Winkler J, Hescheler J, Sachinidis A: Embryonic stem cells for basic...
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Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
... triangles-kinase, circles-others) A solid line indicates a direct interaction while a dashed line indicates an indirect interaction A line without an arrowhead indicates binding and a plus sign indicates ... solid line indicates a direct interaction while a dashed line indicates an indirect interaction A line without an arrowhead indicates binding and a plus sign indicates that othernetworks contain ... while a dashed line indicates an indirect interaction A line without an arrowhead indicates binding and a plus sign indicates that othernetworks contain this gene product Figure Ingenuity pathway...
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Guidelines for Human Embryonic Stem Cell Research potx
... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell ... in which hES cells are introduced into nonhuman primate blastocysts or in which any embryonic stem cells are introduced into human blastocysts (iii) No animal into which hES cells have been introduced ... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html Introduction cell lines demonstrate the need for federal funding of research with NT -derived cell lines or cell...
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Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx
... properties of derived cell types [19] Using genome-wide bisulfite sequencing (BS-seq) [21], we have recently determined the DNA methylation profile of the human embryonic stem cell line HSF1 [22] ... with that in neighboring sequences, defined as ± 500 bp around the binding sites, to assess whether the factor is being affected by specific changes in methylation of the binding site, instead ... counts in the table are compared against the expected values based on the assumption that methylation at C and G is independent Interestingly, we found that, in all cell lines, the methylation in...
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Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot
... target cells that need to be protected against HIV-1 infection Methods hES cell growth and propagation H1 human embryonic stem cell line (hES) was obtained from WiCell (Madison, Wisconsin) The ... hES-CD34+ cells whereas studies of Galic et al [20] derived functional T cells from hES-CD34+ cells in vivo using humanized mice Whether the hES derived T cells support HIV-1 infection remains to ... demonstrated the ability of hES cells to differentiate into a myriad of cell types that include neurons, hematopoietic cells, cardiomyocytes, and insulin-secreting cells, to name a few [3-9] Many...
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Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt
... toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem cells (ESC), mesenchymal stem cells (MSC), and various tissues using all ... J, Skalnik DG: Reduced genomic cytosine methylation and defective cellular differentiation in embryonic stem cells lacking CpG binding protein Molecular and cellular biology 2005, 25:4881-4891 ... genes Int J Cancer 2004, 110:313-319 Amit M, Carpenter MK, Inokuma MS, Chiu CP, Harris CP, Waknitz MA, Itskovitz-Eldor J, Thomson JA: Clonally derived human embryonic stem cell lines maintain pluripotency...
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Human embryonic stem cell derivatives as cancer therapeutics
... stem cell Forward scatter -Galactosylceramide Granulocyte-macrophage colony-stimulating factor Human embryonic stem cell Human embryonic stem cell derived dendritic cell Human embryonic stem cell ... cell derived mesenchymal stem cell Human embryonic stem cell derived neural stem cell Hematopoietic progenitor cell Hematopoietic stem cell Herpes simplex virus Interferon- Immunoglobulin Interleukin ... formation H1 hESCs (WiCell, Madison, WI, USA) were cultured in feeder-free conditions according to the technical manual Maintenance of Human Embryonic Stem Cells in mTeSRTM1 (StemCell Technologies,...
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Dual roles of transcription factor zic3 in regulating embryonic stem cell pluripotency and differentiation
... Introduction 1.1 Derivation of embryonic stem cells 1.2 Regulation of embryonic stem cells 1.2.1 The key properties of ES cells 1.2.2 Extrinsic signalling pathways maintaining ES cell ... following sections 1.2.2 Extrinsic signalling pathways maintaining ES cell pluripotency Embryonic stem cells are maintained by a network of extrinsic and intrinsic signals that collectively regulate ... from cell lines Cell culture Mouse ES cell culture Human ES cell culture Isolation, expansion, and mitotic inactivation of MEF cells Maintenance of HEK293T cells Cryopreservation of cell lines...
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- karyotype and fluorescent in situ hybridization analysis of human embryonic stem cell and induced pluripotent stem cell lines
- clinical and laboratory aspects of preimplantation genetic diagnosis and derivation of affected human embryonic stem cell lines
- steps in excitation contraction coupling in skeletal muscle figures 1 12 and 1 13
- steps in excitation contraction coupling in smooth muscle figure 1 16
- human embryonic stem cell derived cardiomyocytes for cell therapy and drug discovery pdf
- analysis of arrhythmic potential of embryonic stem cell derived cardiomyocytes pdf
- tgf β signaling in embryonic stem cell derived endothelial cells pdf
- development of pacemaker activity in embryonic and embryonic stem cell derived cardiomyocytes
- human embryonic stem cells derived in xeno free conditions
- characterization and gene expression profiling of five human embryonic stem cell lines derived in taiwan
- derivation of human embryonic stem cell lines from poor quality embryos
- derivation expansion and characterization of human embryonic stem cell lines from aneuploid embryos
- chromatin immunoprecipitation based analysis of gene regulatory networks operative in human embryonic stem cells
- stable isotope labelling with amino acids in cell culture for human embryonic stem cell proteomic analysis
- excitation contraction coupling functional properties and autonomic and hormonal regulation in human embryonic stem cell derived cardiomyocytes
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