steps in excitation contraction coupling in smooth muscle figure 1 16

... 48 14 0 50 14 2 14 4 14 6 Time (min) 14 8 15 0 1. 5 2+ [Ca ]c, Z (µM) C 0.5 14 1 14 2 14 3 14 4 14 5 14 6 14 7 14 5 14 6 14 7 14 5 14 6 14 7 Time (min) V (mV) D –40 –50 –60 [Ins (1, 4,5)P3]c, (µM) –70 14 1 14 2 14 3 14 4 ... pacemaking –45 –50 –55 –60 –65 20 40 60 80 10 0 12 0 Time (min) 14 0 16 0 18 0 B 2 20 mV 10 s V (mV) C –55 –60 –65 50 V (mV) D 10 0 15 0 200 Time (min) 250 300 –62 –64 –66 20 40 60 80 10 0 12 0 Time (min) 14 0 ... typically in the range 2–3 mm [26] Finding experimental evidence that electrical coupling is the key ‘spring’ interlinking the Ca2+ stores has involved repeating the decoupling experiment of Fig 1, ...

Ngày tải lên: 16/02/2014, 09:20

... described [12 ,18 ] Freshly isolated cells were phenotypically characterized using muscle- specific antibodies against smooth muscle actin and myosin Cells were maintained in DMEM supplemented with 10 % ... Jasplakinolide hrs 0.5 Swinholide A hrs C CTGF GAPDH 0.5 16 24 hrs 0.5 16 24 hrs LtB + Jasplakinolide Swinholide A +Jasplakinolide actin targets (Fig 6C) In addition, jasplakinolide and swinholide ... Control +S1P +Anisomycin Y-27632 ) ) + – + – + G-Actin/F-Actin 0.260 ± 0.023 0 .18 1 ± 0.023* 0.22 ± 0.0 21 0.239 ± 0. 018 0.223 ± 0. 013 0 .11 0 ± 0.034** 0.227 ± 0. 019   *P < 0.05, **P < 0. 01 versus...

Ngày tải lên: 19/02/2014, 16:20

... branches of rat intrapulmonary arteries [11 -13 ] Compared with P2X receptors much less is known about which of the eight mammalian P2Y subtypes (P2Y1,2,4,6 ,11 ,12 ,13 ,14 ) [14 ,15 ] are expressed in pulmonary ... receptor agonists induce oscillating inward currents ATP, UTP and UDP (10 -4 M) each evoked inward currents (peak amplitude = 13 – 727 pA) in most SPA (n = 11 8) and LPA (n = 11 7) smooth muscle cells ... Edinburgh References 10 11 12 13 14 15 16 Competing interests The author(s) declare that they have no competing interests 17 Authors' contributions KC was involved in the planning of the experiments described...

Ngày tải lên: 12/08/2014, 18:20

... http://respiratory-research.com/content/9 /1/ 76 CF-CXCR2 B CF-CXCR1 60 60 37% 50 40 Counts Counts 40 30 30 20 20 10 10 10 10 10 10 FL1-H 10 10 Control-CXCR1 C 10 10 10 FL2-H 10 Control-CXCR2 D 60 60 50 50 22% 34% 40 40 Counts Counts 16 % ... 9:76 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Boucher RC: Human airway ion transport Part one Am J Respir Crit Care Med 19 94, 15 0:2 71- 2 81 Mitchell I, Corey M, Woenne R, Krastins ... Counts 16 % 50 30 20 30 20 10 10 10 10 10 10 10 10 10 10 FL2-H FL1-H 10 10 Expression of CXCR1/CXCR2 % of positive cells E NS CXCR1 CXCR2 50 NS 40 30 20 10 CF Control Figure Flow cytometric analysis...

Ngày tải lên: 12/08/2014, 14:20

... hypercontractile airway smooth muscle phenotype Eur J Pharmacol 2003, 4 81: 125 -13 1 Page 10 of 11 (page number not for citation purposes) Respiratory Research 2005, 6:85 12 13 14 15 16 17 18 19 20 21 22 23 24 ... platelet-derived growth factor-induced contraction in aortic smooth muscle through inhibition of protein tyrosine kinase(s) J Pharmacol Exp Ther 19 93, 267 :11 19 -11 25 10 11 Berk BC, Alexander RW, Brock TA, Gimbrone ... as the insulinlike growth factor -1 (IGF -1) -receptor, can activate the Rho/Rho-kinase pathway directly [5] and may be involved in smooth muscle contraction via Rho-kinase [6] Smooth muscle contraction...

Ngày tải lên: 12/08/2014, 18:22

... monoclonal anti-(cyclin D1) Ig There was little cyclin D1 protein in SMC stimulated with IL-1b for 24 h (Fig 1C) Incorporating AA before stimulation by IL-1b increased the cyclin D1 concentration, ... cyclin D1 it until it was barely detectable As the retinoblastoma protein, Rb, is a key target of cyclin D1/cyclin-dependent kinase complexes, we investigated whether inhibiting cyclin D1 with ... MAPK kinase (MEK) inhibitor, U 012 6 U 012 6 completely inhibited the cyclin D1 promoter activity in AA-pretreated SMC incubated with IL-1b and reduced it in cells incubated with fetal bovine serum...

Ngày tải lên: 19/02/2014, 16:20

... cells induced by vitamin E-succinate J Cell Sci 11 1, 435–4 41 20 Suzuki, Y.J & Packer, L (19 93) Inhibition of NF-jB activation by vitamin E derivatives Biochem Biophys Res Commun 19 3, 277–283 21 Suzuki, ... 16 Neuzil, J., Weber, T., Terman, A., Weber, C & Brunk, U.T (20 01) Vitamin E analogues as inducers of apoptosis: implications for their potential antineoplastic role Redox Report 6, 14 3 15 1 17 ... Sanders, B.G & Kline, K (19 99) Vitamin E succinate (VES) induces Fas sensitivity in human breast cancer cells: role for Mr 43,000 Fas in VEStriggered apoptosis Cancer Res 59, 953–9 61 11 Neuzil, J.,...

Ngày tải lên: 22/02/2014, 04:20

... ATP 10 12 14 16 18 10 Min 18 (12 ) (55) Control cADPR (1 nM) (40) (11 ) Control amounts of the second messenger cADPR [1, 2] might have an effect on the release of cotransmitters in the smooth muscle ... ATP Ado AMP 10 AMP cADPR, no EFS cADPR, no EFS 12 25 LU ADP 14 16 18 10 16 18 CD38 –/– (12 ) 14 D Spontaneous (PS) ATP overflow (fmol·mg 1 tissue) *** 12 Min CD38 +/+ C Ado AMP ATP Min Spontaneous ... ADP 12 AMP ATP 14 Ado ADP AMP ATP 10 ADP ATP ST, BoNTA Ado β-NAD + ADPR + cADPR 10 0 LU AMP ATP Ado AMP ATP AMP ADP L Durnin and V N Mutafova-Yambolieva 16 18 10 12 Min CD38+/+ C 14 16 18 Min CD38–/–...

Ngày tải lên: 05/03/2014, 23:20

... activated AP -1 [11 13 ] AP -1 is a complex composed of the Fos and Jun proteins [14 16 ] In general, Fos and Jun family proteins function as dimeric transcription factors that bind to AP -1 regulatory ... compilation ª 2009 FEBS No antibody Input 17 23 STAT5B and AP -1 interaction in AGT gene activation A M Han et al +1 AP -1 STAT5 tataaa –282~–277 17 2~ 16 3 TATA AP -1 – 419 ~– 412 B Relative luciferase activity ... pathway induced by acute pressure overload in the rat heart Circ Res 81, 611 – 617 Schieffer B, Bernstein KE & Marrero MB (19 96) The role of tyrosine phosphorylation in angiotensin II mediated intracellular...

Ngày tải lên: 30/03/2014, 02:20

... Journal of Neuroinflammation 2006, 3 :18 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Hendriks L, van Duijn CM, Cras P, Cruts M, Van Hul W, van Harskamp F, Warren A, McInnis MG, Antonarakis ... HS: Aβ-induced chemokine production in primary human macrophages and astrocytes J Neuroimmunol 2002, 12 7 :16 0 -16 8 http://www.jneuroinflammation.com/content/3 /1/ 18 32 33 34 35 36 37 38 39 40 41 42 ... Metalloproteinase inhibition blocks edema in intracerebral hemorrhage in the rat Neurology 19 97, 48:9 21- 926 Frisch SM, Ruoslahti E: Integrins and anoikis Curr Opin Cell Biol 19 97, 9:7 01- 706 Jung...

Ngày tải lên: 19/06/2014, 22:20

... Biochim Biophys Acta 2008, 17 93:223-230 Chen et al Journal of Biomedical Science 2 011 , 18 :2 http://www.jbiomedsci.com/content /18 /1/ 2 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Liu M, Albert ... 10 μM) ± 1- [b-(3-(4-Methoxyphenyl)propoxy)-4-methoxyphenethyl]-1H-imidazole HCl (SKF96365, 10 μM) [13 ,14 ], (9S ,10 R ,12 R)-2,3,9 ,10 ,11 ,12 -hexahydro -10 -methoxy-2,9-dimethyl -1- oxo-9 ,12 -epoxy-1Hdiindolo- [1, 2,3-fg:30,20 ,10 -kl]pyrrolo[3,4-i] [1, 6]benzodiazocine -10 -carboxylic ... 2 011 , 18 :2 http://www.jbiomedsci.com/content /18 /1/ 2 Page 10 of 12 Figure Diagram highlighting protein kinase modulation of SOCs in rat PASMCs Note that protein kinase G (PKG) and protein kinase...

Ngày tải lên: 10/08/2014, 05:21

... http://www.jbiomedsci.com/content /18 /1/ 16 10 11 12 13 14 15 16 17 18 19 20 21 22 Wang R, Xu CQ, Zhao WM, Zhang J, Cao K, Yang BF, Wu LY: Calcium and polyamine regulated calcium-sensing receptors in cardiac tissues ... immunostaining (Figure 1C and 1D) Li et al Journal of Biomedical Science 2 011 , 18 :16 http://www.jbiomedsci.com/content /18 /1/ 16 Page of Figure The calcium sensing receptor (CaSR) is expressed in pulmonary ... changes in patterns of [Ca2+]i induced by 300 μM GdCl3 and various inhibitors were the same as in A Li et al Journal of Biomedical Science 2 011 , 18 :16 http://www.jbiomedsci.com/content /18 /1/ 16 Figure...

Ngày tải lên: 10/08/2014, 05:21

... (rich in activated 27F10-positive inflammatory cells) and in association with clusters of CD68-positive macrophages in deeper medial areas mRNA-expression analysis revealed a distinct increase in ... matrix molecules indicate that although both collagen and elastin production was stimulated in SMC, the even greater increase in MMP activity in both inflammatory cells and SMC resulted in Weissen-Plenz ... Cardiothoracic Surgery 2 010 , 5:66 http://www.cardiothoracicsurgery.org/content/5 /1/ 66 mRNA/GAPDH 2.5 2,5 Page of ctrl CSx 2.0 2,0 1. 5 1, 5 1. 0 1, 0 0.5 0,5 0.0 0,0 MMP1 MMP2 MMP9 Figure Expression of...

Ngày tải lên: 10/08/2014, 09:22

... 2 011 , 8 :18 http://www.journal-inflammation.com/content/8 /1/ 18 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Mahn K, Hirst SJ, Ying S, Holt MR, Lavender P, Ojo OO, Siew L, Simcock DE, McVicker ... Asthmatic group 610 ± 32* 4 61 ± 31* 40 ± 16 * 20 ± 6.3* macrophage 88 ± 15 * Budesonide 372 ± 13 #▲ 14 7 ± 23#▲ 19 ± 3.5#▲ 18 ± 3#▲ 56 ± 10 #▲ 17 2 ± 21 21 ± 7.5 8.2 ± 5.0 0.0 ± 0.0 70 ± 13 treatment group ... Chemokines in asthma: cooperative interaction between chemokines and IL -13 J Allergy Clin Immunol 2003, 11 1:227-242 Riffo-Vasquez Y, Pitchford S, Spina D: Cytokines in airway inflammation Int J...

Ngày tải lên: 11/08/2014, 03:20

... 2 011 , 8 :18 http://www.journal-inflammation.com/content/8 /1/ 18 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Mahn K, Hirst SJ, Ying S, Holt MR, Lavender P, Ojo OO, Siew L, Simcock DE, McVicker ... Asthmatic group 610 ± 32* 4 61 ± 31* 40 ± 16 * 20 ± 6.3* macrophage 88 ± 15 * Budesonide 372 ± 13 #▲ 14 7 ± 23#▲ 19 ± 3.5#▲ 18 ± 3#▲ 56 ± 10 #▲ 17 2 ± 21 21 ± 7.5 8.2 ± 5.0 0.0 ± 0.0 70 ± 13 treatment group ... Chemokines in asthma: cooperative interaction between chemokines and IL -13 J Allergy Clin Immunol 2003, 11 1:227-242 Riffo-Vasquez Y, Pitchford S, Spina D: Cytokines in airway inflammation Int J...

Ngày tải lên: 11/08/2014, 06:23

... Pharmacol 2008, 582 :14 5 -15 3 Oenema et al Respiratory Research 2 010 , 11 :13 0 http://respiratory-research.com/content /11 /1/ 130 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Sato E, Koyama ... involved in the activation of the ERK1/2 pathway in human aortic smooth muscle cells [38] PKC Oenema et al Respiratory Research 2 010 , 11 :13 0 http://respiratory-research.com/content /11 /1/ 130 induces ... http://respiratory-research.com/content /11 /1/ 130 Page of 10 Figure Involvement of the MEK/ERK1/2 pathway in IL-8 release induced by MCh hASMc were stimulated with CSE (5%) (Figure 5A) or IL-1b (1 ng/mL) (Figure 5B), in the absence...

Ngày tải lên: 12/08/2014, 11:22

... culturing the cells on immobilized laminin -11 1 [11 ,22,23] or endogenously produced laminin- 211 [15 ] These inhibitory effects can be reversed using soluble YIGSR [15 ], a binding motif present in ... Migration of eosinophils through matrigel, a basement membrane extract containing laminin -11 1, also requires interaction with b1-integrins [46] These findings suggest that laminin-competing peptides ... critical in maintenance and induction of a (hyper) contractile ASM phenotype Indeed, culturing of ASM cells on a laminin -11 1 matrix inhibits proliferation [11 ,22,23], maintains contractile protein...

Ngày tải lên: 12/08/2014, 13:22

... with the actin crosslinking protein actinin Journal of Surgical Research 2003, 11 1 :15 2 -15 7 An et al Respiratory Research 2 011 , 12 :8 http://respiratory-research.com/content /12 /1/ 8 19 Dreiza CM, ... regulating actin filament dynamics [16 ,18 ], or indirect actions of displacing cofilin alone (Additional file 1, Figure S2) [19 ,20,22] or other regulatory protein kinases/phosphatases that interact ... An integrated strategy for the discovery of drug targets by the analysis of protein-protein interactions International Journal of Mass Spectrometry 2004, 238 :11 9 -13 0 doi :10 .11 86 /14 65-99 21- 12-8...

Ngày tải lên: 12/08/2014, 13:22

... airway remodeling in mice Am J Physiol Lung Cell Mol Physiol 2006, 290: L162 -16 9 Löfdahl et al Respiratory Research 2 011 , 12 :48 http://respiratory-research.com/content /12 /1/ 48 Page 11 of 11 44 Hogg ... were in the lowest staining category (a), and one in the intermediate (b) Page of 11 available stainings for desmin, 17 cases were spindle shaped cells positive both for a-SMA and desmin, and 15 ... obstruction in chronic obstructive lung disease N Engl J Med 19 68, 278 :13 55 -13 60 doi :10 .11 86 /14 65-99 21- 12-48 Cite this article as: Löfdahl et al.: Tenascin-C and alpha -smooth muscle actin positive...

Ngày tải lên: 12/08/2014, 13:22

... Respiratory Research 2 011 , 12 :89 http://respiratory-research.com/content /12 /1/ 89 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 agonists and PGE2 on airway smooth muscle are PKA dependent ... activation within minutes Billington and Hall Respiratory Research 2 011 , 12 :89 http://respiratory-research.com/content /12 /1/ 89 Page of 10 Figure Re-analysis of data shown in figure concentrating on ... activation in airway smooth muscle Respir Res 2006, 7 :11 9 Hall IP, Kotlikoff M: Use of cultured airway myocytes for study of airway smooth muscle Am J Physiol 19 95, 268 (1 Pt 1) :L1 -11 Hall IP,...

Ngày tải lên: 12/08/2014, 13:22