analysis of arrhythmic potential of embryonic stem cell derived cardiomyocytes pdf

... Sachinidis A: Embryonic stem cells: a promising tool for cell replacement therapy J Cell Mol Med 2004, 8:465-473 Winkler J, Hescheler J, Sachinidis A: Embryonic stem cells for basic research and potential ... remarkable mechanisms of cytoprotection and cell survival, also because of the absence of cell division [27] reports Gene Ontology enrichment analysis of the downregulated genes in α-MHC+ cardiomyocytes ... developmental potential of embryonic stem cells Nature 1988, 336:684-687 Klug MG, Soonpaa MH, Koh GY, Field LJ: Genetically selected cardiomyocytes from differentiating embronic stem cells form...

Ngày tải lên: 14/08/2014, 20:22

... Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of ... structures of the peripheral nervous system, such as Schwann and glia cells of the autonomic and enteric nervous systems, endocrine cells, such as the adrenal medulla, and C-cells of the thyroid ... µm 20 µm Analysis the vascular and haematopoietic cell gene markers in the BMP2+ cell population Figure of Analysis of the vascular and haematopoietic cell gene markers in the BMP2+ cell population...

Ngày tải lên: 14/08/2014, 08:20

... for a role in ES cell self renewal Of primary interest are genes whose expressions are restricted to the embryonic stages, as pluripotency is characteristic of embryo derived stem cells The similarity ... seeding densities of 300 and 500 cells per 56cm2 (surface area of each well of a well dish is 56cm2) When 300 cells were reseeded, the number of secondary colonies formed for cells transfected ... cells Put together, all these evidence suggest that the regulation of Lefty1 by OCT4, KLF4 and NANOG may be part of the ES cell self renewal mechanism The role of Lefty1 in regulation of ES cell...

Ngày tải lên: 11/09/2015, 16:02

... embryonic development and stem cell- specific expression of Lefty has been observed in mouse and human, its functional significance in embryonic stem cells has never been demonstrated A part of ... role(s) in embryonic stem cells To elucidate the biological role(s) of Lefty2, loss of function studies using shRNAs was carried out I was able to show that Lefty2 RNAi induces ES cell differentiation ... specification of ES cells is a SMAD3 dependent process On the other hand, SMAD3 plays no role in trophectodermal 158 specification of ES cells as inhibition of SMAD3 activity does not lead to ES cell...

Ngày tải lên: 11/09/2015, 16:02

... ES cells and HEK293 Transfection of plasmids into mouse ES cells and HEK293 cells were performed using Lipofectamine2000 (Invitrogen) 167 4.5.2 Human ES cell transfection For transfection of ... and analysis was carried out on the fourth day of selection 4.9 Alkaline phosphatase staining Detection of alkaline phosphatase, which is indicative of the nondifferentiated state of ES cells, ... 4.14.1 Enhancement of Nodal/Activin signaling E14, TAG1 or tet-Nodal E14tg2a cells were seeded in ES cell medium at a density of 676 cells/cm2 without gelatin The next day, the seeded cells were subjected...

Ngày tải lên: 11/09/2015, 16:02

... pluripotency of human embryonic stem cells in the absence of feeder layers Stem Cells 23, 489-495 Besser, D (2004) Expression of nodal, lefty-a, and lefty-B in undifferentiated human embryonic stem cells ... expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells Cell 113, 643-655 Chambers, I., and Smith, A (2004) Self-renewal of teratocarcinoma and embryonic stem cells Oncogene ... fails to maintain self-renewal of human embryonic stem cells Stem Cells 22, 770-778 Darr, H., Mayshar, Y., and Benvenisty, N (2006) Overexpression of NANOG in human ES cells enables feeder-free growth...

Ngày tải lên: 11/09/2015, 16:02

... 1.1 Embryonic stem cells 1.1.1 Derivation and definition of embryonic stem cells During the transition of an embryo from the morula to the blastocyst ... inefficient clonal propagation etc with stem cells derived from post implantation mouse epiblasts Human ES cells and mouse epiblast derived stem cells also share patterns of gene expression and signaling ... lines of evidence that Nodal signaling mediated by SMAD2/3 indeed plays a role in self renewal and cell fate decisions of pluripotent cell populations in vivo and also that of embryonic stem cells...

Ngày tải lên: 11/09/2015, 16:02

... Subunit of Cadherin protein complex Intracellular mediator of canonical Wnt signaling Non-SET domain protein Histone H3 methyltransferase Transcription factor Intracellular mediator of canonical ... target in mouse Es cells LeftyB (human Lefty1) is regulated by Wnt pathway High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed ... ES cells LeftyA (human Lefty2) is regulated by Wnt pathway Expression detected in ES cells and F9 murine teratocarcinoma Differential expression pattern between ES cells and differentiated cells...

Ngày tải lên: 11/09/2015, 16:03

... Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage embryos, offer a renewable and unlimited source of ... HUMAN EMBRYONIC STEM CELL- DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY (B.Sc (Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... Chapter 1: Introduction 1.1 Neural stem cells 1.1.1 A brief history of NSCs The field of neural stem cell (NSC) biology has its humble beginnings in the second half of the 19th century Several published...

Ngày tải lên: 10/09/2015, 09:08

... Maintenance of pluripotential embryonic stem cells by stem cell selection Reprod Fertil Dev 1998, 10:527-533 53 Wdziekonski B, Villageois P, Dani C: Differentiation of mouse embryonic stem cells and of ... differentiation of embryonic stem cells Curr Opin Cell Biol 1995, 7:862-869 10 Dani C, Smith AG, Dessolin S, Leroy P, Staccini L, Villageois P, Darimont C, Ailhaud G: Differentiation of embryonic stem cells ... the mESC culture system might not only allow the generation of adipose stem cells, but also of their potential vascular niche, opens new avenues for the study of adipose stem cell biology and their...

Ngày tải lên: 09/08/2014, 20:22

... is known about the conservation and variability of DNA methylation across different stem cell lines A recent analysis of about 1% of the genome of HESC lines shows that, by monitoring DNA methylation ... methylation profile of the human embryonic stem cell line HSF1 [22] BS-seq is able to generate genome-wide DNA methylation profiles at single base resolution, much improved from previous profiling ... for providing embryonic stem cell DNA (HSF1) and discussion P-YC is funded by Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA SF is Special Fellow of the Leukemia...

Ngày tải lên: 09/08/2014, 23:20

... hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell- derived NK cells acquire functional receptors ... human embryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Lu S-J, Li F, Vida L, Honig GR: CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic ... Endothelial cells derived from human embryonic stem cells Proc Natl Acad Sci USA 2002, 99:4391-4396 Choi K: The hemangioblast: a common progenitor of hematopoietic and endothelial cells J Hematother Stem...

Ngày tải lên: 14/08/2014, 08:20

... established a cell bank consisting of mouse embry- Page of 18 onic stem (mES) cell clones capable of the inducible overexpression of each one of 32 selected genes, 29 murine orthologs of HSA21 genes ... CD: Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency Stem Cells 2005, 23:663-680 35 Munne S, Howles CM, Wells D: The role of preimplantation ... overexpression of some of the HSA21 genes is due to a high basal expression level of these genes Dosage sensitivity of HSA21 genes in mES cells We further investigated the cause of the lack of a strong...

Ngày tải lên: 09/08/2014, 20:22

... to neural fate by global expression profiling of mouse embryonic stem cells and adult neural stem/ progenitor cells Stem Cells 2006, 24:889-895 NIA array analysis [http://lgsun.grc.nia.nih.gov/ANOVA/ ... al cells Mol Cell Biol 2006, 26:3514-3526 Ohnishi S, Yasuda T, Kitamura S, Nagaya N: Effect of hypoxia on gene expression of bone marrow -derived mesenchymal stem cells and mononuclear cells Stem ... Background Multiple types of adult stem cell exist, of which the hematopoietic stem cell (HSC), which gives rise to cells of all hematopoietic lineages for the life of an animal, is the best...

Ngày tải lên: 14/08/2014, 08:20

... application of the method produces a set of 4,449 cell and tissue markers, including 45 out of 71 known stem cell markers (69%) Analysis of the markers that segregate six types of stem cells (hematopoietic, ... differentiated sets of stem cells We chose six pairs of stem cell samples and their differentiated derivatives from our dataset (two embryonic stem cell lines, hematopoietic stem cells [HSCs], osteoblasts, ... function that endows cells with the property of 'stemness', in the same way that there appears to be no single stemness gene for all stem cells [60] This set of 426 markers of stem cell differentiation...

Ngày tải lên: 14/08/2014, 08:20

... range of threshold values Embryonic stem (ES) cells are derived from the inner cell mass of the embryo and possess the property of pluripotency, which is the ability to develop into any cell lineage ... circuitry in human embryonic stem cells Cell 2005, 122:947-956 Kim J, Chu J, Shen X, Wang J, Orkin SH: An extended transcriptional network for pluripotency of embryonic stem cells Cell 2008, 132:1049-1061 ... culture of pluripotential cells from mouse embryos Nature 1981, 292:154-156 Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM: Embryonic stem cell lines derived...

Ngày tải lên: 14/08/2014, 20:22

... MEF   Mouse embryonic fibroblast xiv  Chapter I – Introduction 1.1 Transcriptional control of stem cell pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, ... referred to as N2B27+BAGC 3.6 Culture of human fetal mesencephalon -derived neural stem cells (ReN-VM) 3.6.1 Maintenance of ReN-VM cells ReN-VM neural stem cells were purchased from ReNeuron (Millipore) ... Maintenance of ReN-VM cells Differentiation of ReN-VM cells 32 32 3.7 Small-interfering RNA (siRNA)-mediated gene silencing 33 3.7.1 3.7.2 Transfection of H1 hESCs with siRNAs Transfection of ReN-VM cells...

Ngày tải lên: 09/09/2015, 17:54

Ngày tải lên: 09/09/2015, 17:54

... profiles of these lncRNAs mirror that of known drivers of neuronal differentiation (Figures 5.2E-F), and were therefore indicative of important roles of the lncRNAs in neurogenesis A group of ... 7.6)   112  Figure 7.5: Schematic representation of differentiation of ReN-VM neural stem cells following transfection of siRNAs Neural stem cells were plated one day prior to siRNA transfection ... knockdown of neuronal lncRNAs The percentage of TUJ1+ cells from Figure 7.6 was quantified by flow cytometry The number of TUJ1+ cells were significantly reduced upon knockdown of any of the four...

Ngày tải lên: 09/09/2015, 17:54

... confirmed by qPCR in two independent cell lines: hESC (H1) -derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC -derived cells and ReN-VM cells, RMST expression was upregulated ... factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST chromatin-immunoprecipitation ... ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas (PFSK-1) indicated a REST binding peak upstream of RMST, suggesting that REST may regulate the transcription of RMST (Figure 8.4)...

Ngày tải lên: 09/09/2015, 17:54