enzyme structure and function

Ngày tải lên: 02/07/2014, 11:20

Ngày tải lên: 30/03/2014, 10:20

... cooperative binding, and creates an accessi- ble environment that also enables Sp1 and Runx1 binding [85,86,233]. Both NFAT and AP-1 have the potential to recruit the HATs CBP and p300 which may ... required for function in either T cells or mast cells. Shown below are the positions that nucleo- somes and GATA-2 occupy in unstimulated T cells and mast cells [85]. Active chromatin and DNase ... exist within CG islands which are characterized by their very high CG content and have an intrinsic resistance to DNA meth- ylation. CG-island promoters tend to be constitutively and ⁄ or ubiquitously...

Ngày tải lên: 14/02/2014, 18:20

... clarified the structural and functional importance of the v-KIND and MAP2 interaction core modules in the regulation of dendrite growth and branching in hippocampal neu- rons and cerebellar granule ... MAP2 cDNA and its derivatives generated by PCR were cloned into pGEX-4T-2. The KIND domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2. The N- and C-terminal ... polymerization, stability and rear- rangement of microtubules in neuronal dendrites [13–15] and is associated with some neurological and psychiatric disorders [16,17]. However, the structure function relationship...

Ngày tải lên: 14/02/2014, 19:20

... from Koehl, 1995). BODY SIZE AND SUSPENSION FEEDING 21 Body Size The Structure and Function of Aquatic Ecosystems Edited by ALAN G. HILDREW School of Biological and Chemical Sciences, Queen Mary, ... SIZE AND SUSPENSION FEEDING 19 Body Size: The Structure and Function of Aquatic Ecosystems Ecologists have long struggled to predict features of ecological systems, such as the numbers and diversity ... important, and (ii) to compare freshwater and marine ecosystems. In Body Size: The Structure and Function of Aquatic Ecosystems, both those questions of scale and comparison among systems are very...

Ngày tải lên: 17/02/2014, 19:20

... Tecnologia). References 1. Davies, D.R. (1990) The structure and function of the aspartic proteinases. Annu. Rev. Biophys. Biophys. Chem. 19, 189–215. 2. Dunn, B.M. (2002) Structure and mechanism of the pepsin-like family ... similar both in sequence and structure to saposin-like proteins. This insert is usually removed during processing and is absent from the mature form of the enzyme. Its functions are still unclear ... preproenzymes and subsequently converted to mature enzymes that can be either single- or two-chain enzymes. The cDNA derived amino acid sequences of several plant APs revealed that the primary structures...

Ngày tải lên: 19/02/2014, 12:20

... investigation and understanding of their structure- activity relationships, may start to provide a rational way to develop additional pharmacological tools for the elucidation of nAChR structure and function. Ó ... perisynaptically, and a less abundant population of a3* nAChRs which contain a5andb4 subunits and in some cases b2 subunits, and is localized at postsynaptic densities [18,96]. Functionally, a ... a-conotoxins MII and AuIB further dissected and correlated the combinato- rial and functional heterogeneity of the slowly decaying population [77]. In this study, two long events of 25 pS 16 and 40 pS...

Ngày tải lên: 19/02/2014, 12:20

... b-sheet face with six b-strands but like sbwAFP the b-strands are very flat. An overlap of the X-ray structure and 3 0 °C NMR structure is shown in Fig. 3C. The secondary structure assignment is ... (1999) Structure, function and evolution of an tifreeze proteins. Cell Mol. Life Sci. 55 , 271 – 283. 11. Yeh, Y. & Feeney, R.E. (1996) Antifreeze proteins: Structures and mechanisms of function. ... [31,32,36] and their three- dimensional structures were determined [34,37–40]. In subsequent sections, we describe the structure and dynamics of each protein, and present a comparison of s bwAFP and TmAFPwitheachotherandwithproteinsthathavea similar...

Ngày tải lên: 19/02/2014, 16:20

... three antiparallel strands (1, 2 and 7) and strand 6, which is parallel to strand 2. b-Strands are located between the four a-helices (a1, 278–294; a2, 305–313; a4, 374–381; and a5, 397–405), with ... b-strands (b3, 329–335; b4, 339–344; and b5, 367–372) and a distorted a-helix (a3, 348–356) (Fig. 3B,C). The three b-strands of the minidomain are all antiparallel, and form a single b -structure. Two ... com- parison of the structure of the protein core (resi- dues 277–328 and 373–413) in solution and in the solid state, and indicates their similarity. A superposition of the C-domain NMR structure on the...

Ngày tải lên: 06/03/2014, 11:20

... between strands E and F in trans- thyretin. The two transthyretin dimers associate, via nonpolar interactions, between the loops joining stands G and H with the loops joining strands A and B, mak- ing ... dublin TLP and zebrafish TLP are found in the flex- ible portions of strands B and C that protrude towards the solvent and in the conformation of the long loop connecting strands D and E [17]. ... regarding the identification and distribution of TLP genes in nature, the structural and functional characterization of the TLP from various organisms, and the evolution of TLP and transthyretin. The...

Ngày tải lên: 07/03/2014, 00:20

... antiparallel b-strands is abutted by three a-helices (a1, a2, and a¢). The b-sheet in type I KH domains consists of three b-strands in the order b1, b¢ and b2. The b1-strand and b2-strand are parallel to ... line connecting the b2-strand and b¢-strand rep- resents the variable loop. The white line connecting the a1-helix and the a2-helix represents the GXXG loop. R. Valverde et al. Structure and function of KH ... other, and the b¢-strand is antiparallel to both (Fig. 1). This all-antiparallel arrangement of strands distinguishes the type I KH fold from the type II KH fold, in which the b1-strand and b2-strand...

Ngày tải lên: 07/03/2014, 05:20

... GlcNAc kinase are labeled with a star. Ó FEBS 2002 Structure and function of GlcNAc kinase (Eur. J. Biochem. 269) 4215 Structure and function of N -acetylglucosamine kinase Identification of two ... C131 and C143. Their counterparts in glucokinase are C233 and C252. Mutations of these cysteines to serines have very similar effects on enzyme activity and substrate binding in glucokinase and ... 5¢-CCCATTCTGAGTGTGGGCTCAGTGTGG Ó FEBS 2002 Structure and function of GlcNAc kinase (Eur. J. Biochem. 269) 4213 The parental template is then digested by the restriction enzyme DpnI, which specifically cuts...

Ngày tải lên: 08/03/2014, 10:20

... double-stranded DNA F plasmid 95,000 Closed-circular double-stranded DNA λ phage 48,500 Linear double-stranded DNA T7 phage 40,000 Linear double-stranded DNA M13 phage 6,400 Closed-circular single-stranded ... phage 3,600 Linear single-stranded RNA Human 6,000,000,000 Linear double-stranded DNA Fruit fly 270,000,000 Linear double-stranded DNA HIV 9,700 Linear single-stranded RNA

Ngày tải lên: 13/03/2014, 16:42

... ring  Nucleoplasm – fluid of the nucleus Nucleoplasm – fluid of the nucleus Cell Structure and Cell Structure and Function Function Why Are Cells So Small? Why Are Cells So Small?  Strategies for ... proteins and Packaging of fully modified proteins and lipids into vesicles for export or use in lipids into vesicles for export or use in the cell the cell  And more that we will not cover! And ... cells  Cell size and shape – Cell size and shape – why are cells so small? why are cells so small?  Prokaryotic cells Prokaryotic cells  Eukaryotic cells Eukaryotic cells  Organelles and structure...

Ngày tải lên: 13/03/2014, 19:31

... fluid Ribosomes Ribosomes Enzymes Enzymes Functions of Functions of Eukaryotic Eukaryotic Cell Features Cell Features Structure Structure Function( s) Function( s) Lysosome Lysosome Contains digestive enzymes ... CELLS CELLS Structure and Function Structure and Function Cell = smallest unit of life Cell = smallest unit of life Functions of Eukaryotic Cell Features Functions of Eukaryotic Cell Features Structure ... from RER RER RER SER SER Functions of Eukaryotic Functions of Eukaryotic Cell Features Cell Features Structure Structure Function( s) Function( s) Cilia and Cilia and Flagella Flagella...

Ngày tải lên: 13/03/2014, 19:31

... Plant g lutathione S-transferases: enzymes with multiple functions in s ickness and in health. Trends Pharmacol S ci 5, 193–198. 4. Marrs, K.A. (1996) The functions and regulation of glutathione S-transferases ... 3505 S -(2,3-Dichlorotriazinyl)glutathione A new affinity label for probing the structure and function of glutathione transferases Georgia A. Kotzia and Nikolaos E. Labrou Laboratory of Enzyme Technology, Department of Agricultural ... nterface and forms part of the h ydro- phobic lock -and- key intersubunit motif. The ability of SDTG to inactivate other glutathione-binding enzymes a nd GST isoenzymes was also investig ated, and it...

Ngày tải lên: 16/03/2014, 16:20

... mitochondrial structure and function. Materials and methods Materials DiS-C 3 (5) and cyclosporin A (CsA) were kindly provided by Hayashibara Biochemical Laboratories, Inc. (Okayama, Japan) and Novartis ... 2004 Effects of DiS-C on mitochondrial structure and function (Eur. J. Biochem. 271) 3577 Multiple effects of DiS-C 3 (5) on mitochondrial structure and function Takenori Yamamoto 1,2 , Aiko Tachikawa 1,2 , ... found DiS-C 3 (5) to show multiple effects on the mitochondrial structure and function, effects dependent on both its concentration and the P i status. 11 Acknowledgements This work was supported...

Ngày tải lên: 16/03/2014, 18:20

Ngày tải lên: 23/03/2014, 06:20

Ngày tải lên: 23/03/2014, 13:20

Ngày tải lên: 23/03/2014, 15:20