... recruitment of
MOF to recently transcribed regions, especially at the
3¢ ends of genes where H3-K36me3 is enriched [128].
In mammalian cells MOF also exists as partof a sepa-
rate MOF–MSLv1 complex ... with
binding of both MOF and Tip60 (equivalent to Esa1
in yeast NuA4) [124,130]. However, in human cells
depletion of MOF, but not Tip60, results in reduced
global levels of AcH4-K16 and defective ... acetylation and deacetyla-
tion, and transient recruitment of remodellers and
transcription factors.
A cycle of transcription commences with the recruit-
ment of transcription factors and co-factors...
... of dendritic arborization
patterns
To quantify dendrite growth and branching, 15 neurons
transfected with each construct were randomly chosen for
each experiment, and EGFP fluorescent images of ... MAP2 cDNA and its derivatives
generated by PCR were cloned into pGEX-4T-2. The KIND
domains of Spir-2 and PTPN13 were generated by RT-PCR
and inserted into pEGFP-C1 and pGEX-4T-2. The N- and
C-terminal ... stability and rear-
rangement of microtubules in neuronal dendrites
[13–15] and is associated with some neurological and
psychiatric disorders [16,17]. However, the structure–
function relationship of...
... predicting,
measuring and understanding the roles of different kinds of organisms in the flux
and storage of elements in ecosystems. The total biomass per unit area, W,is
simply the sum ofthe body mass of all ... NC 28557 and Virginia Institute of
Marine Science, The College of William and
Mary, Gloucester Point, VA 23062.
Michael C. Marshall Department of
Zoology and Physiology, University of
Wyoming, ... Zealand.
Lusha M. Tronstad Department of
Zoology and Physiology, University of
Wyoming, Laramie, WY 82071, USA.
Philip Warren Department of Animal
and Plant Sciences, University of Sheffield,
Western...
... superposition of the exponential rise to analyze
quantitatively the contribution ofeach phase. The
amplitude and rate constant ofeachof the three
phases can be calculated by deconvolution of the
traces ... use of FI in the study of PSII electron transport
and to obtain a more complete analysis of the O–J,
J–I and I–P rises. This method was also used to find
the apparent activation energy ofeach ... type of nonlinear regression
shows that the method can be used as an excellent
approximation of FI traces and to quantitatively esti-
mate the contribution ofeach phase. The average val-
ues of...
... extent of membrane
localization of Noxo1b and Noxo1c. The
intensities of immunoreactive bands for
HA-Noxo1b and HA-Noxo1c in (B) were
quantified using a LAS-1000plus (Fuji film)
image analyzer and ... activity of the PX
domains of Noxo1b and Noxo1c
The membrane localization of Noxo1b is mediated in
part by binding of the PX domain to membrane phos-
pholipids [19]. Less association of Noxo1c ... SH3 domain of Noxo1 participates in
regulation of Nox3, the role of the PX domain in Nox3
activity remains unknown.
In the process of cloning of human Noxo1, some
spliced transcripts of the NOXO1...
... prosegment and total or partial removal of the internal
Fig. 1. Plant aspartic proteinase precursors. Comparison of the amino
acid sequences of representative members of the A1 family of plant
aspartic ... The structure andfunctionof the aspartic
proteinases. Annu. Rev. Biophys. Biophys. Chem. 19, 189–215.
2. Dunn, B.M. (2002) Structure and mechanism of the pepsin-like
family of aspartic peptidases. ... by the prosegment, but also by
the 13 residues of the N-terminal of the mature enzyme and
by the ÔflapÕ. The anchorage of the prosegment andofpart
of the N-terminus in the active site cleft is...
... domain ofeach subunit contri-
butes to the formation of the hydrophilic pore. ACh binding protein
has structural and functional homology to the extracellular ligand
binding domain of the nAChR, and ... based on
single channel recordings and the use of a-conotoxins MII
and AuIB further dissected and correlated the combinato-
rial and functional heterogeneity of the slowly decaying
population ... the identification and
characterization of a-conotoxins with selectivity for neur-
onal nAChR subtypes and provides examples of their use in
defining the compositions andfunctionof neuronal nAChR
subtypes...
... de note the five residues of on e of the three
sides of s bwAFP o r s ix residues of on e of two sides of T mAFP. T he
strands that make up t he three, parallel b-sheets of t he pro tein are
designated ... addition of an
additional one, two or three b-helical c oils compared to the
shorter isoforms. In the case of one sbwAFP isoform,
named CfAFP-501, a detailed e xamination of t he structure
and function ... D. Sykes
CIHR Group in Protein Structure and Function, Department of Biochemistry and Protein Engineering Network of Centres of
Excellence, University of Alberta, Edmonton, Alberta, Canada
Antifreeze...
... activation andfunctionof the cytokine Spa
¨
tzle
in the innate immune response of a lepidopteran insect,
Manduca sexta
Chunju An
1
, Haobo Jiang
2
and Michael R. Kanost
1
1 Department of Biochemistry, ... by
recognition of microbial patterns and by microbial
proteinases. Further biochemical and genetic research
is required for a more complete understanding of the
extracellular reactions of plasma proteins ... collected, and fat body RNA samples were prepared from each insect.
(A) Antimicrobial activity of plasma assayed against E. coli, and identification of antimicrobial plasma proteins by SDS ⁄ PAGE and...
... minidomain and the zinc-binding domain
of the zinc finger protein Ynr046w [25] (Fig. 8). The fit
of the heavy atoms (Ca, C, and N) from three
b-strands and the a-helices of both the closed and the
open ... variation of pH in the range
between 6.3 and 7.7, of ionic strength between 25 and
100 mm NaCl andof temperature between 278 and
313 K did not lead to any detectable change in the
populations of the ... that of the corresponding part of
the crystal structure [19]. Four b-strands (b1, 301–303;
b2, 320–323; b6, 389–392; and b7, 409–412) form a
b-sheet with three antiparallel strands (1, 2 and 7) and
strand...
... dublin TLP and zebrafish TLP are found in the flex-
ible portions of strands B and C that protrude towards
the solvent and in the conformation of the long loop
connecting strands D and E [17]. ... present and discuss recent find-
ings regarding the identification and distribution of
TLP genes in nature, the structural and functional
characterization of the TLP from various organisms,
and the ... Interruptions in the b-strands A, G
and H are also observed in TLP structures as a result
of alterations to the formation of hydrogen bonds
between strands. The carbonyls of residues V104 and
P105 (zebrafish...
... ARTICLE
Synthesis andfunctionof ribosomal proteins – fading
models and new perspectives
Sara Caldarola, Maria Chiara De Stefano, Francesco Amaldi and Fabrizio Loreni
Department of Biology, University ... maturation and export of
the two ribosomal subunits [1]. Most of the constitu-
ents of the preribosomal particles have been identified
in yeast by exploiting the potent combination of
genetic and biochemical ... steps of ribosome biogenesis
and ⁄ or ribosome function. Indeed, a systematic study
of the incorporation of RPs into preribosomes led to
the identification of the in vivo assembly pathway of
the...
... which the b1-strand and b2-strand are adjacent
and parallel to each other, and the b¢-strand is adja-
cent and antiparallel to the b1-strand (Fig. 1). The
length and sequence of the variable ... syndrome and
paraneoplastic disease, are associated with the loss offunctionof a particular
KH domain. Here we discuss the progress made towards understanding both
general and specific features of ... b-sheet
composed of three antiparallel b-strands is abutted by
three a-helices (a1, a2, and a¢). The b-sheet in type I
KH domains consists of three b-strands in the order
b1, b¢ and b2. The b1-strand and...
... part that holds two of the Fe-S clusters in the small subunit of
the D. gigas enzyme is missing in HoxY of the SH, suggesting an
exposure ofpartof the hydrophobic surface of the HoxH subunit. ... resulted in the total loss of this
activity when assayed in the usual way, i.e. after addition
of H
2
, a catalytic amount of NADH and the subsequent
addition of BV. Restoration of this activity (to ... effect of
FMN, but not of FAD or riboflavin, has been noticed
earlier [36,37].
Figure 4 shows the effect of the FMN concentration
on the reconstitution of the activity of the reduced SH.
Addition of...
... labeled
with a star.
Ó FEBS 2002 Structure andfunctionof GlcNAc kinase (Eur. J. Biochem. 269) 4215
Structure andfunction of
N
-acetylglucosamine kinase
Identification of two active site cysteines
Markus ... characterization of GlcNAc kinase
and cysteine mutants
In order to get a more detailed insight into the functions of
specific cysteine residues of GlcNAc kinase, the K
m
values
for both substrates, GlcNAc and ... using
the
RASMOL
software.
RESULTS
Construction of cysteine mutants and functional
expression in
E. coli
BL21
In an earlier study, the use of specific thiol-modifying
chemical reagents revealed the presence of...