name and function of each part

... recruitment of MOF to recently transcribed regions, especially at the 3¢ ends of genes where H3-K36me3 is enriched [128]. In mammalian cells MOF also exists as part of a sepa- rate MOF–MSLv1 complex ... with binding of both MOF and Tip60 (equivalent to Esa1 in yeast NuA4) [124,130]. However, in human cells depletion of MOF, but not Tip60, results in reduced global levels of AcH4-K16 and defective ... acetylation and deacetyla- tion, and transient recruitment of remodellers and transcription factors. A cycle of transcription commences with the recruit- ment of transcription factors and co-factors...

Ngày tải lên: 14/02/2014, 18:20

... of dendritic arborization patterns To quantify dendrite growth and branching, 15 neurons transfected with each construct were randomly chosen for each experiment, and EGFP fluorescent images of ... MAP2 cDNA and its derivatives generated by PCR were cloned into pGEX-4T-2. The KIND domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2. The N- and C-terminal ... stability and rear- rangement of microtubules in neuronal dendrites [13–15] and is associated with some neurological and psychiatric disorders [16,17]. However, the structure– function relationship of...

Ngày tải lên: 14/02/2014, 19:20

... predicting, measuring and understanding the roles of different kinds of organisms in the flux and storage of elements in ecosystems. The total biomass per unit area, W,is simply the sum ofthe body mass of all ... NC 28557 and Virginia Institute of Marine Science, The College of William and Mary, Gloucester Point, VA 23062. Michael C. Marshall Department of Zoology and Physiology, University of Wyoming, ... Zealand. Lusha M. Tronstad Department of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA. Philip Warren Department of Animal and Plant Sciences, University of Sheffield, Western...

Ngày tải lên: 17/02/2014, 19:20

... superposition of the exponential rise to analyze quantitatively the contribution of each phase. The amplitude and rate constant of each of the three phases can be calculated by deconvolution of the traces ... use of FI in the study of PSII electron transport and to obtain a more complete analysis of the O–J, J–I and I–P rises. This method was also used to find the apparent activation energy of each ... type of nonlinear regression shows that the method can be used as an excellent approximation of FI traces and to quantitatively esti- mate the contribution of each phase. The average val- ues of...

Ngày tải lên: 19/02/2014, 05:20

... extent of membrane localization of Noxo1b and Noxo1c. The intensities of immunoreactive bands for HA-Noxo1b and HA-Noxo1c in (B) were quantified using a LAS-1000plus (Fuji film) image analyzer and ... activity of the PX domains of Noxo1b and Noxo1c The membrane localization of Noxo1b is mediated in part by binding of the PX domain to membrane phos- pholipids [19]. Less association of Noxo1c ... SH3 domain of Noxo1 participates in regulation of Nox3, the role of the PX domain in Nox3 activity remains unknown. In the process of cloning of human Noxo1, some spliced transcripts of the NOXO1...

Ngày tải lên: 19/02/2014, 06:20

... prosegment and total or partial removal of the internal Fig. 1. Plant aspartic proteinase precursors. Comparison of the amino acid sequences of representative members of the A1 family of plant aspartic ... The structure and function of the aspartic proteinases. Annu. Rev. Biophys. Biophys. Chem. 19, 189–215. 2. Dunn, B.M. (2002) Structure and mechanism of the pepsin-like family of aspartic peptidases. ... by the prosegment, but also by the 13 residues of the N-terminal of the mature enzyme and by the ÔflapÕ. The anchorage of the prosegment and of part of the N-terminus in the active site cleft is...

Ngày tải lên: 19/02/2014, 12:20

... domain of each subunit contri- butes to the formation of the hydrophilic pore. ACh binding protein has structural and functional homology to the extracellular ligand binding domain of the nAChR, and ... based on single channel recordings and the use of a-conotoxins MII and AuIB further dissected and correlated the combinato- rial and functional heterogeneity of the slowly decaying population ... the identification and characterization of a-conotoxins with selectivity for neur- onal nAChR subtypes and provides examples of their use in defining the compositions and function of neuronal nAChR subtypes...

Ngày tải lên: 19/02/2014, 12:20

... de note the five residues of on e of the three sides of s bwAFP o r s ix residues of on e of two sides of T mAFP. T he strands that make up t he three, parallel b-sheets of t he pro tein are designated ... addition of an additional one, two or three b-helical c oils compared to the shorter isoforms. In the case of one sbwAFP isoform, named CfAFP-501, a detailed e xamination of t he structure and function ... D. Sykes CIHR Group in Protein Structure and Function, Department of Biochemistry and Protein Engineering Network of Centres of Excellence, University of Alberta, Edmonton, Alberta, Canada Antifreeze...

Ngày tải lên: 19/02/2014, 16:20

... activation and function of the cytokine Spa ¨ tzle in the innate immune response of a lepidopteran insect, Manduca sexta Chunju An 1 , Haobo Jiang 2 and Michael R. Kanost 1 1 Department of Biochemistry, ... by recognition of microbial patterns and by microbial proteinases. Further biochemical and genetic research is required for a more complete understanding of the extracellular reactions of plasma proteins ... collected, and fat body RNA samples were prepared from each insect. (A) Antimicrobial activity of plasma assayed against E. coli, and identification of antimicrobial plasma proteins by SDS ⁄ PAGE and...

Ngày tải lên: 06/03/2014, 09:22

... minidomain and the zinc-binding domain of the zinc finger protein Ynr046w [25] (Fig. 8). The fit of the heavy atoms (Ca, C, and N) from three b-strands and the a-helices of both the closed and the open ... variation of pH in the range between 6.3 and 7.7, of ionic strength between 25 and 100 mm NaCl and of temperature between 278 and 313 K did not lead to any detectable change in the populations of the ... that of the corresponding part of the crystal structure [19]. Four b-strands (b1, 301–303; b2, 320–323; b6, 389–392; and b7, 409–412) form a b-sheet with three antiparallel strands (1, 2 and 7) and strand...

Ngày tải lên: 06/03/2014, 11:20

... dublin TLP and zebrafish TLP are found in the flex- ible portions of strands B and C that protrude towards the solvent and in the conformation of the long loop connecting strands D and E [17]. ... present and discuss recent find- ings regarding the identification and distribution of TLP genes in nature, the structural and functional characterization of the TLP from various organisms, and the ... Interruptions in the b-strands A, G and H are also observed in TLP structures as a result of alterations to the formation of hydrogen bonds between strands. The carbonyls of residues V104 and P105 (zebrafish...

Ngày tải lên: 07/03/2014, 00:20

... ARTICLE Synthesis and function of ribosomal proteins – fading models and new perspectives Sara Caldarola, Maria Chiara De Stefano, Francesco Amaldi and Fabrizio Loreni Department of Biology, University ... maturation and export of the two ribosomal subunits [1]. Most of the constitu- ents of the preribosomal particles have been identified in yeast by exploiting the potent combination of genetic and biochemical ... steps of ribosome biogenesis and ⁄ or ribosome function. Indeed, a systematic study of the incorporation of RPs into preribosomes led to the identification of the in vivo assembly pathway of the...

Ngày tải lên: 07/03/2014, 01:20

... which the b1-strand and b2-strand are adjacent and parallel to each other, and the b¢-strand is adja- cent and antiparallel to the b1-strand (Fig. 1). The length and sequence of the variable ... syndrome and paraneoplastic disease, are associated with the loss of function of a particular KH domain. Here we discuss the progress made towards understanding both general and specific features of ... b-sheet composed of three antiparallel b-strands is abutted by three a-helices (a1, a2, and a¢). The b-sheet in type I KH domains consists of three b-strands in the order b1, b¢ and b2. The b1-strand and...

Ngày tải lên: 07/03/2014, 05:20

... part that holds two of the Fe-S clusters in the small subunit of the D. gigas enzyme is missing in HoxY of the SH, suggesting an exposure of part of the hydrophobic surface of the HoxH subunit. ... resulted in the total loss of this activity when assayed in the usual way, i.e. after addition of H 2 , a catalytic amount of NADH and the subsequent addition of BV. Restoration of this activity (to ... effect of FMN, but not of FAD or riboflavin, has been noticed earlier [36,37]. Figure 4 shows the effect of the FMN concentration on the reconstitution of the activity of the reduced SH. Addition of...

Ngày tải lên: 07/03/2014, 15:20

... labeled with a star. Ó FEBS 2002 Structure and function of GlcNAc kinase (Eur. J. Biochem. 269) 4215 Structure and function of N -acetylglucosamine kinase Identification of two active site cysteines Markus ... characterization of GlcNAc kinase and cysteine mutants In order to get a more detailed insight into the functions of specific cysteine residues of GlcNAc kinase, the K m values for both substrates, GlcNAc and ... using the RASMOL software. RESULTS Construction of cysteine mutants and functional expression in E. coli BL21 In an earlier study, the use of specific thiol-modifying chemical reagents revealed the presence of...

Ngày tải lên: 08/03/2014, 10:20

Ngày tải lên: 08/03/2014, 22:20

Ngày tải lên: 16/03/2014, 16:20

Ngày tải lên: 22/03/2014, 16:20

Ngày tải lên: 23/03/2014, 15:20

Ngày tải lên: 29/03/2014, 20:20