nature of law role and function of law in society

Ngày tải lên: 13/08/2014, 16:20

... full-length v-KIND; DKIND1, deletion of KIND1; DKIND2, deletion of KIND2; DKIND1 + 2, deletion of both KIND1 and KIND2; DRasN, deletion of RasN; DGEF, deletion of RasGEF; KIND1, KIND1 domain; KIND2, KIND2 ... v-KIND KIND2 binding resides within residues 702–744 of MAP2. Residues 456–487 in the KIND2 domain of v-KIND contain the core MAP2 binding module To identify the core MAP2-binding site within ... sequence for v-KIND binding. To evaluate the v-KIND KIND2-binding specificity of residues 702–744 of MAP2, we performed a pull- down assay of combinations of EGFP-fused KIND1 or KIND2 of v-KIND with...

Ngày tải lên: 14/02/2014, 19:20

Ngày tải lên: 29/03/2014, 20:20

Ngày tải lên: 09/08/2014, 20:20

Ngày tải lên: 10/08/2014, 20:20

Ngày tải lên: 16/12/2013, 14:45

... [238–240]. Even in the absence of pro- tein–protein interactions, factors can assist each other’s binding to DNA within nucleosomes. This principle is illustrated in the model shown in Fig. 7A. In this ... considerable varia- tion and complexity in the number of nucleosomes dis- rupted, the histone composition of the remaining nucleosomes, and the number and nature of protein complexes they interact with. Much ... transcribed regions of active genes in human T cells [124]. In a study in yeast, mutations were introduced alone or in combination in lysines 5, 8, 12 and 16 in the gene for histone H4 [125]. Of these,...

Ngày tải lên: 14/02/2014, 18:20

... predicting, measuring and understanding the roles of different kinds of organisms in the flux and storage of elements in ecosystems. The total biomass per unit area, W,is simply the sum ofthe ... size, in comparison to a linear increase in gain as the driving force for the development of gelatinous bodies. As seen above, however, the same principle of costs increasing more rapidly than gains ... 28557 and Virginia Institute of Marine Science, The College of William and Mary, Gloucester Point, VA 23062. Michael C. Marshall Department of Zoology and Physiology, University of Wyoming, Laramie,...

Ngày tải lên: 17/02/2014, 19:20

... (PX) domain in the N-terminus. PX domains occur in a variety of proteins involved in cell signaling, mem- brane trafficking, and polarity establishment, and func- tion as phosphoinositide-binding modules ... in the testis and fetal brain. The Noxo1c protein contains an additional five amino acids in the N-terminal PX domain, a phosphoinosi- tide-binding module; the domain plays an essential role in ... domains with p22 phox [25– 27]. Although the SH3 domain of Noxo1 participates in regulation of Nox3, the role of the PX domain in Nox3 activity remains unknown. In the process of cloning of human...

Ngày tải lên: 19/02/2014, 06:20

... and C-terminal domain in red. (B) Ribbon representation of the structure of the PSI domain of barley prophytepsin [25] (N-terminal domain, blue; C-terminal domain, red). (C) Model structure of ... family includes saposins, which are lysosomal sphingolipid-activator pro- teins [37], NK-lysin, granulysin, surfactant protein B, amoebapores and domains of acid sphingomyelinase and acyloxyacyl hydrolase ... folding, stability and intracellular sorting of several zymogens [16], the PSI is an insertion only identified in plant APs, which is highly similar to saposins and saposin-like proteins and whose biological...

Ngày tải lên: 19/02/2014, 12:20

... structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors. Nature 411, 269–276. 6. Dutertre,S.&Lewis,R.J.(2004)Computationalapproachesto understand a-conotoxin interactions ... (Table 1). Interestingly, Vc1.1 was able to inhibit in vivo a vascular response to pain and was effective in alleviating chronic pain and accelerating functional recovery in an animal model of neuropathy. ... transmembrane domain of each subunit contri- butes to the formation of the hydrophilic pore. ACh binding protein has structural and functional homology to the extracellular ligand binding domain of the...

Ngày tải lên: 19/02/2014, 12:20

... freeze-intolerant insects The structure and function of b-helical antifreeze proteins Steffen P. Graether and Brian D. Sykes CIHR Group in Protein Structure and Function, Department of Biochemistry and Protein ... so, a considerable number of questions remain before we can solve the interaction at the atomic level and understand t he role of the threonine side chains in ice binding. The contradiction between ... f isoform 337 with the structure o f th e l onger i soform CfAFP-501 using the main chain of residues Thr23fiAsn90 in i soform 337 and residues Thr54fiMet121 in CfAFP-501. (B) Overlap of isoforms...

Ngày tải lên: 19/02/2014, 16:20

... contained a 30 bp insertion in the ORF, beginning at nucleotide 516. This resulted in insertion of a 10 amino acid segment (TREIDYPETI) and one substitution (Ser fi Gly) at the C-terminal end of ... residue cystine-knot domain. Injection of Spa ¨ tzle-C108, but not proSpa ¨ tzle-1A, into larvae stimulated expression of several antimicrobial peptides and proteins, including attacin-1, cecropin-6, moricin, lysozyme, ... development and in the antimicrobial immune response in larvae and adults. We have investigated the function of Spa ¨ tzle in a lepidopteran insect, Manduca sexta, in which hemolymph proteinases activated...

Ngày tải lên: 06/03/2014, 09:22

... minidomain and the zinc-binding domain of the zinc finger protein Ynr046w [25] (Fig. 8). The fit of the heavy atoms (Ca, C, and N) from three b-strands and the a-helices of both the closed and the open ... specificity of the factor. The results provide new insights into the possible role of the C-domain in the process of translation termi- nation. Abbreviations C-domain, C-terminal domain (or domain 3) of ... most of the residues of the protein rigid core could be successfully fitted using models 1 and 2. For a few residues of the minidomain (330–332, at the beginning of the central b-strand) and residues...

Ngày tải lên: 06/03/2014, 11:20

... various organisms, and the evolution of TLP and transthyretin. The identification of TLPs and transthyretins in nature The evolution of transthyretin and its distribution in nature have been well ... b-strands A, G and H [17]. Structural nature of the TLP and transthyretin active sites One of the striking features of transthyretin is the cen- tral channel of the protein into which the THs bind. This ... transthyretin dimers associate, via nonpolar interactions, between the loops joining stands G and H with the loops joining strands A and B, mak- ing the transthyretin tetramer a ‘dimer of dimers.’ Recently,...

Ngày tải lên: 07/03/2014, 00:20

... FEBS synthesis involving small modifying peptides (ubiquitin and NEDD8) affecting protein stability. The most intriguing recent finding is the phenotype resulting from mutations in RPs in zebrafish, mice, and ... elusive. In Xenopus , two proteins have been identified, La and cellular nucleic acid-binding protein (CNBP) ⁄ zinc finger protein 9 (ZNF9), which bind the 5¢-UTRs of RP mRNAs in vitro. La interacts ... mitogen-induced signal transduction path- ways acting through TSC1–TSC2 and involving mTORC1, as suggested by the rapamycin effect. Rapamycin, which inhibits mTORC1 by binding to mTOR in a complex...

Ngày tải lên: 07/03/2014, 01:20

... poly(C)-bind- ing proteins: a multiplicity of functions and search for mechanisms. RNA 8, 265–278. 32 Gamernik AV & Andino R (2000) Interactions of viral protein 3CD and poly(rC) binding protein ... nucleotide at the 3¢-end of the ssDNA strand (dC in 2AXY; dT in 2PQU) is participating in base-stacking interactions with the preceding cytosine base. Structure and function of KH domains R. Valverde ... sequences. Although in both examples the coupling of two RNA-binding domains will effectively increase the specificity and affinity of the RNA–protein interaction, the two different binding modes have...

Ngày tải lên: 07/03/2014, 05:20

... work and [36,37]). (f) It is proposed that the FMN-a is bound to the inwards-pointing end of the flavodoxin fold in the HoxY subunit. Such a flavodoxin fold is conserved in the small subunit of all ... part that holds two of the Fe-S clusters in the small subunit of the D. gigas enzyme is missing in HoxY of the SH, suggesting an exposure of part of the hydrophobic surface of the HoxH subunit. ... used in all experiments. The purity of the samples was examined by SDS/PAGE [29]. Protein concentrations were routinely determined by the Bradford method [30] using bovine serum albumin as a standard. Activity...

Ngày tải lên: 07/03/2014, 15:20

... Irina Druzhinina 1 and Christian P. Kubicek 1 1 Division of Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, TU Wien, Vienna; 2 Institute of Ecology, University of ... significant differences in amino acids flanking the active site. The 3D model of Lad1 was fitted, according to the 2D structure of SDH [25]. Amino acids involved in binding of D -sorbitol [25] and conserved between ... alignment of mammalian and fungal sorbitol dehydrogenases. Amino acids involved in binding of D -sorbitol [25] are marked by asterisks, amino acids conserved between Lad1 and SDH in % are indicated...

Ngày tải lên: 07/03/2014, 15:20

... align- ments of glucokinase and GlcNAc kinase and the common ATP-binding domain, the GlcNAc kinase was fitted into the glucokinase model using RasMol software. Figure 5 shows the predicted structure of ... alignment of glucokinase and GlcNAc kinase. The amino acid sequences are numbered beginning with the first amino acid of the N-terminus. Both amino acid sequences share the ATP binding subdomains (Phosphate1, ... for hexokinase [30]. Binding of the sugar results in a strong conforma- tional change allowing the binding of ATP. Mutations of amino acids involved in this mechanism therefore result in lower...

Ngày tải lên: 08/03/2014, 10:20