dynamic expansion culture for mesenchymal stem cells
Dynamic mechanical stimulation for mesenchymal stem cell chondrogenesis in an elastomeric scaffold
... particular that of stem cells, and the biomaterials, for cartilage tissue engineering will be addressed 2.3.1 Stem cell based Approaches for Cartilage Tissue Engineering The aforementioned weaknesses ... encourage the investigation on stem cells with chondrogenic potential for articular cartilage repair Both pluripotent and multipotent stem cells have been reported for studying cartilage repair(Hwang ... outcome from BMSCs treatment Pluripotent cells utilized in cartilage tissue engineering include embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) (Medvedev et al., 2010,Oldershaw...
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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt
... human mesenchymal stem cells Science 284, 143–147 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pluripotent population with multiple applications Curr Stem Cell ... marrowderived mesenchymal stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan Z (2009) Paracrine action mediates the antifibrotic effect of transplanted mesenchymal ... to hypoxia ⁄ SD for h The cells were then fixed in 2% paraformaldehyde in NaCl ⁄ Pi for 30 min, washed twice with NaCl ⁄ Pi and permeabilized with 0.3% Triton X-100 in NaCl ⁄ Pi for 10 Next, the...
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Báo cáo y học: "Local adherent technique for transplanting mesenchymal stem cells as a potential treatment of cartilage defect" pdf
... (page number not for citation purposes) vial mesenchymal stem cells into cartilage defect Application of low-invasive local adherent technique to transplant synovial mesenchymal stem cells into cartilage ... 24 25 26 Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Prockop DJ: Marrow stromal cells as stem cells for nonhematopoietic tissues Science 1997, 276:71-74 ... remove nonadherent cells, and the adherent cells were cultured for days as passage without refeeding The cells were then trypsinized, harvested and resuspended to be used for transplantation...
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Báo cáo y học: "Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix" ppsx
... AD-MSCfor 3D co cultured alone Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stem cells (AD-MSC) and annulus cells compared with separate culture of AD-MSC ... matrix formed by sand rat adipose-derived mesenchymal extracellular 3D factor in the Immunohistochemical documentation ofstem cellsgrowthculture beta sand rat adipose-derived mesenchymal stem cells ... lineage specific differentiation satisfy the standard criteria suggested for defining mesenchymal stem cells Mesenchymal stem cells should characteristically show positive localisation of CD44, CD29,...
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Báo cáo y học: " Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment" pdf
... canine adipose tissue derived mesenchymal stem cells In order to demonstrate that the cells isolated from the canine adipose tissue (Figure 1A) are indeed mesenchymal stem cells (MSCs) we differentiated ... enabling co -culture induced chondrogenesis in MSCs A: Fourteen days high-density culture Untreated MSC cultures became apoptotic (a) In primary chondrocyte cultures (b), co-cultures (c), co-cultures ... chondrocyte cultures and untreated co-cultures To further demonstrate the influence of curcumin on the induction of the apoptotic signalling cascade by IL-1β in co-cultures, cultures were evaluated for...
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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
... 43 2.3 Stem cells 51 2.3.1 Definition and classification 51 2.3.2 Stem cells in tissue engineering 53 2.3.3 Mesenchymal stem cells 54 v 2.4 Stem cell ... effectively induce osteogenic differentiation of stem cells without the need for osteogenic solutes The scaffolds were enriched with mesenchymal stem cells (MSCs) to provide the osteogenic property ... ACID)-BASED NANOFIBERS FOR OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS NGUYEN THI HIEN LUONG (B.Eng., Ho Chi Minh city University of Technology, Vietnam) A THESIS SUBMITTED FOR THE DEGREE...
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Transplantation of mesenchymal stem cells for the treatment of parkinsons disease in a mouse model
... bone marrow mesenchymal stem cells Society for Neuroscience 35th Annual Meeting, Washington DC, USA Chao YX, He BP, Tay SSW (2006) Immune Potential of Mesenchymal Stem Cells Society for Neuroscience ... for in vitro expansion has made it difficult to use broadly in the future clinical study Mesenchymal stem cells (MSCs) represent one of the most accessible sources of multipotent stem cells for ... replacement as a possible strategy for the CNS is worth considering because stem cells could differentiate into neural cells Neural stem cells would be good candidate for cell replacement in neurodegenerative...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal stem cells ... human mesenchymal stem cells from marrow Tissue Eng (4):415-428 129 Beresford JN, Graves SE, Smoothy CA 1993;Formation of mineralized nodules by bone derived cells in vitro: a model of bone formation? ... the proliferation and differentiation of rat mesenchymal stem cells Stem Cells Dev (4):661-670 69 Edlund U, Dånmark S, Albertsson AC 2008;A strategy for the covalent functionalization of resorbable...
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Characterization of adult human bone marrow mesenchymal stem cells for effective myocardial repair
... Stem cells 1.5.1.1 Embryonic stem cells 1.5.1.2 ES cell stem therapy is not ideal for clinical use 1.5.2 Adult stem cells 10 1.5.2.1 Bone marrow stem cells ... Bone marrow stem cells There are most common types of stem cells in bone marrow: (i) hematopoietic stem cells and (ii) mesenchymal stem cells These cell types are believed to be ideal for myocardial ... has since been believed that hematopoietic stem cells may not differentiate into cardiomyocytes45 1.5.2.1.2 Mesenchymal stem cells Mesenchymal stem cells (MSCs) encompass a heterogeneous population...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... stromal progenitor cells (mesenchymal progenitor cells) : implications for therapeutic use Bone Marrow Transplant,1995,16(4):557-564 Aggarwal S, Pittenger MF Human mesenchymal stem cells modulate ... application of stem cells provides promise for the treatment of ischemic femoral head necrosis When compared with vascular intervention and local drilling for injection, transplantation with stem cells ... Z, Chen B, et al Ex vivo expansion and in vivo infusion of bone marrow-derived Flk-1+CD31-CD34- mesenchymal stem cells: feasibility and safety from monkey to human Stem Cells Dev,2006,15(3):349-357...
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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood Stem Cells 22, 625–634 ... differentiation from stem cells For microarray analysis, we confirmed the hepatic differentiation of AT-MSC into hepatocyte-like cells using the original protocol (Fig 1A) The differentiated cells (AT-MSC-Hepa) ... hepatic cells, the findings presented here suggest that MET might be a pivotal factor in determining stem cell transdifferentiation Discussion AT-MSCs may be good candidates as stem cells for cell...
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báo cáo hóa học:" Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures" potx
... adherent cells when isolated from hFTs (primary cultures) Morphology of adherent cells when isolated from hFTs (primary cultures) A): Cells cultured for three days after initial plating Cells with ... phenotype and a small cluster of cells with endothelial appearance (arrows) (100×) B): Cells cultured for six days after initial plating (100×) C) Cells cultured for six days after initial plating ... Hayward, CA) Unstained cells were gated on forward scatter to eliminate particulate debris and clumped cells A minimum of 5.000 events were counted for each sample Mesenchymal Stem Cell Differentiation...
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Báo cáo hóa học: " Adipose-derived mesenchymal stem cells markedly attenuate brain infarct size and improve neurological function in rats" pptx
... adipose-derived mesenchymal stem cells (ADMSCs) for the treatment of stroke has seldom been discussed [19] Compared with embryonic stems cells and bone marrow-derived mesenchymal stem cells, ADMSCs ... transplanted cells into the host tissue [3] Interestingly, while stem cell therapy, including bone marrow-derived mesenchymal stem cells [15-17], embryonic stem cells [14] and endothelial progenitor cells ... embryonic stem cell, neuron stem cells, bone marrow-derived mesenchymal stem cell, and peripheral blood-derived stem cells have been frequently investigated for their feasibility and safety in...
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Báo cáo hóa học: "Early combined treatment with sildenafil and adipose-derived mesenchymal stem cells preserves heart function in rat dilated cardiomyopathy" doc
... integration of the transplanted cells and the host tissue As compared with embryonic stems cells and bone marrow-derived mesenchymal stem cells, adiposederived mesenchymal stem cells (ADMSCs) have the ... J, Ratajczak MZ: Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis Stem Cells 2005, 23:879-894 Kee HJ, Sohn IS, ... collection: in vivo therapeutic potential of human adipose tissue mesenchymal stem cells after transplantation into mice with liver injury Stem Cells 2008, 26:2705-2712 Behling A, Rohde LE, Colombo FC,...
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Báo cáo sinh học: " Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats" potx
... model performed in rats Methods Animal ethics The investigation conformed to the Principles of Laboratory Animal Care formulated by the National Society for Medical Research and the Guide for the ... W, Chen J, Cong X, Hu S, Chen X: Hypoxia and serum deprivationinduced apoptosis in mesenchymal stem cells Stem Cells 2006, 24:416-425 Sun Y, Jin K, Xie L, Childs J, Mao XO, Logvinova A, Greenberg ... The supernatant from MSCs cultured in normal condition was used for control Any experiment was repeated for three times Page of 10 corresponding to coronal coordinates form bregma -1 to mm were...
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Báo cáo sinh học: "Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model" potx
... Lee JW: Therapeutic potential of mesenchymal stem cells for severe acute lung injury Chest 2010, 138:965-972 20 Chistiakov DA: Endogenous and exogenous stem cells: a role in lung repair and use ... endotoxin-induced systemic response by bone marrow-derived mesenchymal stem cells in mice Am J Physiol Lung Cell Mol Physiol 2007, 293:L131-141 23 Salgar S, Manning E, Ruiz P, Pham S: Mesenchymal Stem Cell ... although bone marrowderived MSC is the major source of stem cells in these studies [22,24-27], the therapeutic role of adiposederived mesenchymal stem cells (ADMSCs) in acute IR injury of the lung has...
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báo cáo hóa học:" Elevated adipogenesis of marrow mesenchymal stem cells during early steroid-associated osteonecrosis development" pptx
... USA) The cells were cultured in an incubator at 37°C, 5% humidified CO2 for two weeks Then the cells were harvested for the following evaluations: MSCs adipogenesis evaluation After plating cells ... MSCs harvest and culture After dynamic MRI measurement at week 2, the bone marrow was harvested from proximal femur for MSCs culture based on our established protocol [12] MSCs were cultured in basal ... http://www.josr-online.com/content/2/1/15 Dynamic- MRI for vessels perfusion function Dynamic MRI for bilateral proximal femora and distal femora was done before LPS injection (week 0), one week (week...
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o cáo hóa học:" Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats" docx
... model performed in rats Methods Animal ethics The investigation conformed to the Principles of Laboratory Animal Care formulated by the National Society for Medical Research and the Guide for the ... W, Chen J, Cong X, Hu S, Chen X: Hypoxia and serum deprivationinduced apoptosis in mesenchymal stem cells Stem Cells 2006, 24:416-425 Sun Y, Jin K, Xie L, Childs J, Mao XO, Logvinova A, Greenberg ... The supernatant from MSCs cultured in normal condition was used for control Any experiment was repeated for three times Page of 10 corresponding to coronal coordinates form bregma -1 to mm were...
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báo cáo hóa học:" Targeting of mesenchymal stem cells to ovarian tumors via an artificial receptor" docx
... Stem Cells 2008, 26(9):2332-8 11 Xin H, Kanehira M, Mizuguchi H, Hayakawa T, Kikuchi T, Nukiwa T, Saijo Y: Targeted delivery of CX3CL1 to multiple lung tumors by mesenchymal stem cells Stem Cells ... targeted cell vehicles Much of the groundwork for such targeting approaches has previously been established for immune cells (T -cells, NK cells, CIK cells) , where lymphocyte populations were modified ... marrow-derived mesenchymal stem cells in the treatment of gliomas Cancer Res 2005, 65(8):3307-18 Studeny M, Marini FC, Champlin RE, Zompetta C, Fidler IJ, Andreeff M: Bone marrow-derived mesenchymal stem cells...
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