division of labour in animal cells
Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
... confirmed by sequencing DNA samples of 51 healthy individuals. Interestingly, this SNP had not yet been registered in any SNP databases despite of its high frequency of 20% in samples of patients ... of samples containing the C/T SNP downstream of exon 10 led to the identification of cleavage products of approximately 101 bp and 444 bp in size in samples either containing the heterozygous ... (SNP) in the intron sequence flanking the 3’ end of exon 10 in 7 out of 35 (20%) patient samples. The SNP consisted of a C > T exchange (Figure 1 C-E) and was positioned at nt 67 of the intron...
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The noneffects of class on the gendered division of labor in the home
... mode of production level of analysis, involving comparisons of capitalist class structures with different kinds of noncapitalist classstructures, or at a more concrete level of analysis, involving ... gender is the problem of bargaining power between men and women within households. Parti-cularly in an era in which gender roles are being challenged, the division of labor in the household should ... andgender. Instead of asking how the gender division of labor withinfamilies varies across locations within a class structure, the focus of analysis would be on how the gender division of labor...
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Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells pdf
... was obtained with scFv4A-d1-EGFP and 7A-d1-EGFP expressed in parallel.Interestingly, in the case of 4A (but not 7A), we founda minor percentage of cells with a faint cytoplasmicstaining (arrow). ... FRAP:measuring protein mobility and activity in living cells. Nature Cell Biol 3, 145–147.34 Bachmair A, Finley D & Varshavsky AK (1986) In vivohalf-life of a protein is a function of its amino-terminalresidue. ... fused to d1-EGFP are maintained in the cells by binding to antigen.Generation of soluble binding-defectiveand cysteine-deleted mutantsTo demonstrate that the observed intracellular colocal-ization...
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
... POUhomeodomain family proteins bind to this consensus motifand regulate gene transcription in diverse cells throughinteraction with a variety of partner DNA binding proteins,including Sp1 family members ... thepivotal role of fgl2 in a murine model for murine hepatitisvirus strain 3 (MHV-3) induced fulminant hepatic failure[21,22]. Administration of neutralizing antibodies againstfgl2 conferred ... development of a normal fetus and in regulatingimmune responses within the intestine. Induction of fgl2 in macrophages in response to viruses results in theproduction of a potent coagulant activity...
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Báo cáo khoa học: Ca2+/calmodulin-dependent kinase II signalling cascade mediates P2X7 receptor-dependent inhibition of neuritogenesis in neuroblastoma cells docx
... withuntreated cells. As expected, cells exposed to KN93also developed more neurites than control cells, point-ing to a key role of this kinase in the control of neuritogenesis in N2a cells (Fig. 5E).Involvement ... currents in this cell line. Pharmacological inhibition of the P2X7 receptor and its knockdown by small hairpin RNA interferenceresulted in increased neuritogenesis in cells cultured in low serum-contain-ing ... adhesion kinase is upstream of phosphati-dylinositol 3-kinase ⁄ Akt in regulating fibroblast survival in response to contraction of type I collagen matricesvia a beta 1 integrin viability signaling...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... many proteins involved in apoptosis, including the expression profiles of proteins involved in the mito-chondrial (bcl-2, bax, and bcl-x, etc.) and stress reac-tion-related pathways of apoptosis ... transfected cells and control cells. Interestingly, the phosphorylation of JNK/SAPK was inhibited by the Int. J. Med. Sci. 2007, 4 40Figure 2 PowerBlot patterns of JAR cells. Forty elements of ... overexpressed in cytotrophoblasts, and a decrease in the expression of Bcl-2 and increase of apoptotic cells are seen in preeclampsia. Thus antiapoptotic proteins can play an important role in preventing...
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Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
... signaling. This finding corroborates the interaction observed between various intracellular signaling pathways and apoptosis induced by 1mM concentration of ethanol in HepG2 cells [19]. Int. ... . Interestingly, HMGN3 has been found to be associated with resistance against anticancer drugs including vinblastine, topotecan, paclitaxel and doxorubincin in human hepatocellular carcinoma ... (including Kupffer cells) [41, 42], and ethanol-induced mucosal injury in the upper gastrointestinal tract leading to increase in the permeability of the gut mucosa to endotoxins [43, 44]. These...
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Báo cáo y học: " Low concentration of ethanol induce apoptosis in HepG2 cells: role of various signal transduction pathways"
... its initial effect on protein kinases phosphorylation. The most strikingly and novel finding obtained hereby was the strong inhibition of the GPCR signaling pathway induced mainly by increased ... of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54. 24. Pelech S, Sutter C, Zhang H. Kinetworks protein kinase multiblot analysis. ... Kinne RKH. Effects of doxorubicin, mitomycin C, and ethanol on Hep-G2 cells in vitro. J Cancer Res Clin Oncol 1999;125:1-8. 26. Higashi K, Hoshino M, Nomura T, et al. Interaction of protein...
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Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
... expression of claudin-1 in the cytoplasm, with some nuclear expression of claudin-1. B) Treatment of these cells with PKA inhibitor results in nuclear translocation of claudin-1 in as little as 15 minutes, ... is involved in claudin-1 expression and localization. Indeed, a study shows that increasing β-catenin in- creases the expression of claudin-1 in colorectal cancer cells, and that claudin-1 ... lo-calization of claudin-1. Nuclear claudin-1 does not increase melanoma cell motility. We have previously shown that increasing the levels of CLDN-1 increases the invasion of melanoma cells (4)....
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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... long-chain saturatedfatty acids (SFAs) led to increased expression of proin-flammatory cytokines, inhibition of insulin signaling,induction of endoplasmic reticulum (ER) stress, andpromotion of ... carcinoma cells were grown in DMEM containing 10% heat-inactivated fetal bovineserum, 2 mm glutamine, 100 UặmL)1penicillin, and100 ngặmL)1streptomycin, at 37 C in air with 5% CO2. Cells ... in two phases according to the intensityand the duration of the stress. An initial adaptiveresponse culminates in the temporary inhibition of protein synthesis, providing cells with the opportunityto...
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