distance information for disordered proteins from nmr and esr measurements using paramagnetic spin labels
nmr methods for the investigation of structure and transport
... sensitivity and information content in NMR spectra Molecular biology and medicine were revolutionized by the advent of multidimensional NMR spectroscopy and NMR imaging Apart from chemical analysis and ... Dr Nestle and Dr Wassmer from BASF SE, Ludwigshafen, and Ms Herold Technical assistance from Mr Oliver and the workshops is gratefully acknowledged Forschergruppe 338 der Deutschen Forschungsgemeinschaft ... field Spin density Discrete spin density Discrete spin density with artifacts Constant spin density Threshold for spin density Binary filtered Matrix Q/nm Filtered Matrix Q0 /nm Discrete spin...
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Báo cáo y học: "Investigating the complementary value of discrete choice experiments for the evaluation of barriers and facilitators in implementation research: a questionnaire survey" pptx
... receive information from the surgeon before discharge Patients should receive information from the anesthesiologist before discharge Patients should be visited by the breast care nurse before discharge ... elsewhere [8] Therefore, we will now present the DCE method to the extent that the information is relevant for understanding of the present paper For more theoretical background information about ... oncologist and breast cancer nurse are both responsible for information about diagnosis and treatment options (preoperative counselling) Oral counselling after the diagnosis is supported by written information...
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báo cáo khoa học: " Investigating the complementary value of discrete choice experiments for the evaluation of barriers and facilitators in implementation research: a questionnaire survey" pot
... receive information from the surgeon before discharge Patients should receive information from the anesthesiologist before discharge Patients should be visited by the breast care nurse before discharge ... elsewhere [8] Therefore, we will now present the DCE method to the extent that the information is relevant for understanding of the present paper For more theoretical background information about ... oncologist and breast cancer nurse are both responsible for information about diagnosis and treatment options (preoperative counselling) Oral counselling after the diagnosis is supported by written information...
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Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc
... atnos for automated NOESY cross-peak picking and NOE signal identification [77], candid for NOESY cross-peak assignment and calibration of NOE-derived upper distance constraints [78], and dyana for ... cross-peaks originating from helices and 3, as well as from residues between helix and the tight loop, were least affected and remained at levels between 20% and 50% The paramagnetic relaxation ... secondary structure and 16% turn formed, whereas only 40% of unordered conformations remained A NMR spectroscopy and resonance assignment The experimental conditions for the NMR study of VpUcyt...
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Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc
... FSBG-targeted proteins Overlaying an image of the immunoblot with the 2D pattern of silver-stained proteins pointed to candidate ATP-binding or GTPbinding proteins that were selected from the 2D gel and ... Fund for Scientific Research ) Flanders (Belgium) The work in this paper was supported by research grants from the Fund for Scientific Research ) Flanders (Belgium) (project number G.0280.07) and ... modifications ) protein processing [34,36] and N-glycosylation [39] – and describe the use of COFRADIC for studying interactions between small molecules and proteins The latter is a particular application...
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Tài liệu Mesoscopic Model for Mechanical Characterization of Biological Protein Materials docx
... Cα atoms i and j are connected by an entropic spring with force constant K if the equilibrium distance rij0 between two Cα atoms i and j is less than the cut-off distance rc RESULTS AND DISCUSSIONS ... other hand, for the case (ii) where parallel bonds reside in the polymer chain, the force exerted for each bond in parallel configuration is given by f = F/NB The probability to withstand the force ... interatomic potential in the form of Eq (4) consists of potential for backbone chain stretching and the potential for native contacts Go potential is a versatile model for protein modeling such...
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Báo cáo khoa học: Affinity purification-mass spectrometry Powerful tools for the characterization of protein complexes ppt
... art for the biochemical retrieval of associated proteins and protein complexes from cells and tissues as well as their analysis by mass spectrometry, for it is this combination of biochemistry and ... particularly useful for the study of model organisms for which only limited sequence information on the protein level is available Even in cases where no sequence information is available, tandem mass ... availability of protein and nucleotide sequence information has made mass spectrometry and biology much more compatible than in the past The Ôjoined forcesÕ of sophisticated biochemical and mass spectrometry...
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Báo cáo sinh học: " Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" pptx
... CA, USA) and CD45 (clone TU116, BD Biosciences) by incubating the cells in 100 μL PBS for 10’ at 4°C Cells were then washed with PBS, and fixed with 1% formaldehyde for 20’ at 4°C before permeabilization ... methods (positive selection, CD45 depletion and the combination of both) in a spiking experiment model using a cell line known to be positive for EpCAM, and CK and We observed the highest recovery in ... measurements, performed the statistical analysis and drafted the manuscript AF conceived the study, designed and conducted the study and drafted the manuscript AS collected the samples and reviewed...
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báo cáo hóa học:" Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" docx
... CA, USA) and CD45 (clone TU116, BD Biosciences) by incubating the cells in 100 μL PBS for 10’ at 4°C Cells were then washed with PBS, and fixed with 1% formaldehyde for 20’ at 4°C before permeabilization ... methods (positive selection, CD45 depletion and the combination of both) in a spiking experiment model using a cell line known to be positive for EpCAM, and CK and We observed the highest recovery in ... measurements, performed the statistical analysis and drafted the manuscript AF conceived the study, designed and conducted the study and drafted the manuscript AS collected the samples and reviewed...
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báo cáo khoa học: "Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay" docx
... were purchased from Fisher Scientific Preparation of buffer solutions and stock solutions All buffer solutions were prepared and pH-adjusted using sodium hydroxide (10 M, M, and M) and hydrochloric ... approximately 20 mM and the mixture was left to stand for 10 minutes The activated QDs were purified of unreacted reagents and byproducts by dialysis using 100 kDa MWCO microcentrifuge tubes and re-suspended ... serum albumin (BSA) and horse radish peroxidase (HRP) [7], and QD-conjugates with Ulex europaeus (UEA-1) and anti-von Willebrand factor (anti-vWF) [32] CE has also been used for immunoassays involving...
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báo cáo khoa học: " A new model for the characterization of infection risk in gunshot injuries:Technology, principal consideration and clinical implementation" pptx
... out CT and CBCT scans for clinical and systematic analyses; but the potential for systematic testing offered by gelatin blocks is far from having been fully exploited The present paper therefore ... at 120 kV and 200 mA Analysis The data obtained were stored in a digital format (DICOM) and transferred to a personal computer for further analysis Statistical analyses were performed using the ... Sources of support and financial interest: none Authors’ contributions CS, MR, MS, CW, MRu, and NCG conceived of the work and participated in its design and coordination CS and MR made substantial...
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Báo cáo y học: "Novel multiplex technology for diagnostic characterization of rheumatoid arthritis" pdf
... correlation between the results from the multiplex assay and those from the single assay was good, with correlation coefficients of 0.92 for RF and 0.97 for CRP (Figures 2b and 2c) Analysis of the bone-turnover ... obtaining informed consent from the patients and under human subjects protocols approved by the Stanford University Institutional Review Board Samples from RA patients were obtained from ARAMIS ... calculated on the basis of the standard curves generated Results for the synovial antigen and chips (for which standards have not yet been generated) were reported and analyzed as signal intensities...
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IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE
... were performed using QuickChange (Qiagen) mutagenesis ALDH3A1 mutants were constructed using forward primer 5’–CTT CAA CCT CAC CAT CGC GCC CAT GGT GGG CGCC–3’ and complement for Q122A and forward ... chemotherapeutic agent and in the mobilization and conditioning regimens for blood and marrow transplantation Reviewing the chemistry and pharmacology of cyclophosphamide is crucial for understanding its ... mustard and its mechanism of DNA cross linking Figure represents how phosphoramide mustard forms intrastrand and interstrand cross–links between guanine bases from DNA 23 B Hypothesis and approaches...
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guide for structural maintenance of parking structures
... housekeeping, and aesthetic maintenance are discussed in Chapter Chapter provides a checklist for maintenance tasks and recommended frequencies Appendices A and B contain information about snowplowing and ... specifications; a set of as-built drawings; product information, including warranty and maintenance information from the manufacturers of various components; and specific maintenance requirements If a ... can reduce the need for significant and expensive repairs This guide is intended for owners, operators, and consultants for parking structures who seek advice on developing and implementing a...
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Application of biased scanning probe microscopy techniques for multifunctional characterization of bifeo3 and zno thin films
... hysteresis loop Forward and reverse coercive voltages, V+ and V−, nucleation voltages Vc+ and Vc− , + − and forward and reverse saturation and remnant responses, R0 , R0 , Rs+ and Rs− , are shown ... amplitude (dzz) and Surface potential results for all the samples under stress and magnetic field at location and Table 6.1 Mean domain angle under the unbiased and biased area for undoped and 2%Mg ... amplitude and phase for (a) undoped and (b) 4% Mg doped BLFO samples (1µm scan size) Figure 6.2 Local hysteresis loop for domain switching and corresponding strain loop for (a) undoped BLFO and (b)...
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Structural characterization of nogo proteins implications for biological functions and moleculedesign of therapeutic applications
... unfolded proteins and provides dynamics information on a residue-by-residue basis Characteristic chemical shift deviations of Hα, Cα, and Cβ from random coil values are good indicators for the ... electric field and produces a very efficient mechanism for nuclear spin relaxation and leads to NMR signal broadening and even no NMR signal could be detected In an external magnetic field, spin of ... isoforms, termed Nogo-A, -B and –C, by alternative promoter usage and /or splicing Nogo-A, -B and –C proteins and mRNAs are expressed ubiquitously, although there is some tissue specificity For...
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Tài liệu ComProtect® Solid-State Single-Pair Protectors for Series 2 Switching Blocks docx
... rain, fungus, airborne contaminants and aging Also meets standards for environmental cycling for impulse and AC surges • Meets GR-63 standards for transport and storage • NEBS Level compliant ... be isolated and easily replaced • Meets all standards for controlled environments including temperature, humidity, office vibration and airborne contaminants • Meets all standards for uncontrolled ... bars Package of 25 Black housing 6659 008-30 Yellow housing 6659 008-90 Red housing 6659 008-50 Package of 100 Black housing 6659 010-30 Yellow housing 6659 010-90 Red housing 6659 010-50 Ground...
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Fundamentals of quantum mechanics for solid state electronics, optics c tang
... Hamiltonian and the commutator and it is of a very different form from Newton’s equation of motion for x or vx , which involves the force On the other hand, Heisenberg’s equation is of the form of ... operators is a non-zero constant For example, ^ ^ from Eqs (2.11), the uncertainty product of x and px is always non-zero, and from (2.12): ÁxÁpx ! "=2; similarly for the y and z coordinates: h (2:13) ... useful tools for arriving at the expansion of state functions in different bases such as the form (2.28) and (2.29) or (2.28a) and (2.29a) For example, applying the unit operators (2.41) and (2.42)...
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nanomaterials for solid state hydrogen storage, 2009, p.346
... safe and efficient production of hydrogen gas At present, 48% of hydrogen is produced from methane steam reforming, 30% from oil/naphtha reforming, 18% from coal gasification, and only 3.9% from ... Therefore, foundations for hydrogen storage and for formation of fine and ultrafine-grained microstructures in solid materials had been developing concurrently At that time when world emerged from ... bottom Force (kgf ) 10 Left top Right top 0 10 12 14 16 Distance (mm) Fig 1.9 The attractive force between the 25-mm steel ball metal vs distance from the cylinder (working distance – WD) and four...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008