discovery and expression profiling
Báo cáo sinh học: " Cloning and expression profiling of the VLDLR gene associated with egg performance in duck (Anas platyrhynchos)" docx
... population mean; Si and Gj are the fixed effects of each generation and genotype, respectively, and εij is the random error Values are considered significant at P < 0.05 and are presented as ... tissue, and there was no obvious differential expression in heart and brain tissues Considering that the differential expression of both VLDLR variants varies slightly among species and tissues, ... tissue, intestine, pituitary gland, ovary and oviduct, immediately frozen in liquid nitrogen and stored at -80° C until total RNA extraction Ducks of the second (n = 350) and third (n = 251) generations...
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gene expression profiling, methods and protocols
... Grand Island, NY) E coli DNA ligase (BRL, Grand Island, NY) E coli DNA polymerase (BRL, Grand Island, NY) E coli RNase H (BRL, Grand Island, NY) Arctic shrimp alkaline phosphatase (USB, Cleveland, ... relationship among internal standards within the SMIS and between each internal standard and its respective cDNA from a sample The internal standard for each reference gene and target gene is at a ... (endpoint) even though the band intensity for both NT and CT is low at early cycle compared to late cycle Similarly, the target gene NT band and CT band are about equivalent and the real-time value...
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báo cáo hóa học:" Gene expression profiling for molecular distinction and characterization of laser captured primary lung cancers" ppt
... (overexpression in AC vs NT, SCC vs NT and SCLC vs NT was 2.2, 2.1 and 3.2-fold, respectively); collagen, type I, alpha (overexpression in AC vs NT, SCC vs NT and SCLC vs NT was 7.98, 3.24 and ... SCC and SCLC to normal lung tissue using significance analysis of microarrays (SAM), we found 205, 335 and 404 genes with an at least 2-fold different expression level and an estimated false discovery ... of microtubules and intermediary filaments, as well as keratin and 15 We also looked for genes involved in cell adhesion and migration and found integrin alpha 3, integrin beta and intercellular...
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Báo cáo hóa học: " cDNA targets improve whole blood gene expression profiling and enhance detection of pharmocodynamic biomarkers: a quantitative platform analysis" ppt
... study design, participated in the expression profiling assays and participated in the ex vivo dosing study DA participated in the expression profiling assays and participated in the ex vivo dosing ... achieve and inadvertent delays in processing time can lead to processing biases that can reduce discovery power of expression profiles [12] To improve the laboratory assays and increase discovery ... to changes in expression of thousands of genes [9,12,13] Another challenge is that the specificity and sensitivity of a given RNA profiling platform are affected by the abundance and variability...
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Báo cáo y học: "Perspectives and limitations of gene expression profiling in rheumatology: new molecular strategies" doc
... in the exchange and development of different approaches to gene expression profiling and in comparing selected candidates This will improve our concepts to overcome the problems and limitations ... for RANTES and MCP-2, and for T-cell migration only for RANTES The expression of GCP-2 and MCP-2, which have not yet been investigated in RA, was identified in both synovial tissue and synovial ... precursor, K-cadherin, cyclin G1, DNA polymerase and the tumour-associated glycoprotein E4 By expression profiling in experimental SLE of NZB/W mice, Alexander and colleagues [20] identified endogenous...
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Báo cáo y học: "Gene expression profiling in murine autoimmune arthritis during the initiation and progression of joint inflammation" pps
... clusters C and D showed an expression peak later, at the acute phase of inflammation (Fig 5), and encoded chemokine receptors (Ccr2 and Ccr5) and chemokine ligands (Cxcl1, Ccl2, Ccl7, and Ccl9) ... C and D also included interferon-activating genes Ifi203, Ifi47, and Ifigtp, and cell differentiation antigens such as CD48 and CD53 Hierarchical clusters E and F contained four genes whose expression ... cellular and tissue specificity of gene expression, using public gene expression databases [54-56], indicated that genes encoding CD48 (Cd48), membrane-spanning 4A6B and 4A6C (Ms4a6b and Ms4a6c),...
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Báo cáo y học: "Metalloproteinase and inhibitor expression profiling of resorbing cartilage reveals pro-collagenase activation as a critical step for collagenolysis" pot
... performed the cartilage assays, and helped conceive, design, and coordinate the study and draft the manuscript ADR and TEC helped conceive, design, and coordinate the study and draft the manuscript ... are the mean ± standard error of the mean = control; ▲ = IL-1+OSM PCR Furthermore, we have correlated this gene expression with gelatinase and collagenase enzyme expression and activation, as ... extracted from cartilage, and TIMP-1, -2, -3, and -4, RECK, and α2M gene expression determined by real-time polymerase chain reaction (n = 3) as described in Materials and methods The data are...
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Báo cáo khoa học: "Gene expression profiling of human dermal fibroblasts exposed to bleomycin sulphate does not differentiate between radiation sensitive and control patients" pptx
... cellular localization and cell death (Table 2) and for down-regulated genes included regulation of progression through cell cycle, mitotic phase of cell cycle and DNA damage response and repair (Table ... to cause both G1/S and G2/M arrest [21] At high doses, extensive double strand DNA breaks and apoptosis occur [22] We aimed to use a dose at which post-mitotic differentiation and cellular response ... in the study design and statistical analysis, carried out cell and RNA preparation and drafted the manuscript KKS participated in study design, performed statistical analysis and helped to draft...
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Báo cáo y học: "Microarray gene expression profiling of osteoarthritic bone suggests altered bone remodelling, WNT and transforming growth factor-β/bone morphogenic protein signalling" pot
... analysis of WNT5B, ITGB2, MMP25 and SMAD3 expression between females and males in OA bone For each gene, a graph depicts relative MMP25 and SMAD3 expression between females and males in OA bone real-time ... example, ANXA2, GSN, ITGB2, FOXP1 and PDE4A) and osteoblast (LTF, DF and TGFB1) function and hence bone remodelling (Tables and 6) Collectively, the differential expression of these genes is consistent ... protein kinases (AKT3 and PRKCD), calcium and metal ion binding proteins (S100A4, S100A6, MT1L, MT2A and MT1G), transcription factors (TWIST1, FMR2, KLF6, NR4A2 and DEC1), and both enzymatic (ADAMTS4,...
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Báo cáo y học: "Early and long-standing rheumatoid arthritis: distinct molecular signatures identified by gene-expression profiling in synovia" pot
... basis of tissue-preferred expression in liver and corresponded to genes with liver-restricted expression (10% of the probes) and genes with combined hepatic and broad expression (the other 90%) ... analyze and interpret biological data, draft the manuscript, and perform the statistical analyses OV and JPS helped conceive of the study and participated in its design and coordination and helped ... analyze and interpret biological data and draft the manuscript XLL helped conceive of the study and participated in its design and coordination and helped draft the manuscript MH, XA, NB, IAA, and...
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Báo cáo y học: "Candidate genes for alcohol preference identified by expression profiling in alcohol-preferring and -nonpreferring reciprocal congenic rats" ppsx
... steps, and dashed lines represent genes that are functionally related Identified candidate genes are in boxes; gray color indicates a lower expression in iP and white color indicates higher expression ... mining millions of expression profiles–database and tools Nucleic Acids Res 2005, 33:D562-566 Edgar R, Domrachev M, Lash AE: Gene Expression Omnibus: NCBI gene expression and hybridization array ... The dopamine and serotonin biosynthesis and other pathways - for example, the Nfkb1 pathway - were overrepresented Six candidate genes, including Akr1b1, Qdpr, Snca, Spr, Scap2, and Copg2, are...
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Báo cáo y học: "Integrated miRNA and mRNA expression profiling of mouse mammary tumor models identifies miRNA signatures associated with mammary tumor lineage" ppt
... in miRNA expression between the normal mammary glands and mammary tumors, we compared the miRNA expression profiles across all of the murine tumor models and normal mammary glands miRNA expression ... Figure Over -expression of (a) miR-494 and (b) miR-412 inhibits expression of Birc4 and Bmpr1a, respectively M6 cells and DB-7 cells were transduced with lentivirus expressing miR-494 and miR-412, ... experiments, analyzed and interpreted data and drafted the manuscript CHK performed the microarray experiments and helped with quality control and analysis MY and RS normalized the data and performed...
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Báo cáo y học: "Integrated miRNA and mRNA expression profiling of mouse mammary tumor models identifies miRNA signatures associated with mammary tumor lineage" docx
... in miRNA expression between the normal mammary glands and mammary tumors, we compared the miRNA expression profiles across all of the murine tumor models and normal mammary glands miRNA expression ... Figure Over -expression of (a) miR-494 and (b) miR-412 inhibits expression of Birc4 and Bmpr1a, respectively M6 cells and DB-7 cells were transduced with lentivirus expressing miR-494 and miR-412, ... experiments, analyzed and interpreted data and drafted the manuscript CHK performed the microarray experiments and helped with quality control and analysis MY and RS normalized the data and performed...
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báo cáo khoa học: "EST sequencing and gene expression profiling of defence-related genes from Persea americana infected with Phytophthora cinnamomi" pot
... gene expression analysis Expression analyses of nine genes were conducted at 0, 3, 6, 12, 24 and 48 hours post infection (hpi) to validate if the pyrosequencing data reflected their gene expression ... increase in expression at 12 hpi The highest level of up-regulation achieved was 1.1 and 1.47 respectively (Figure 4h &4i) Table Similarities between pyrosequencing data and gene expression profiles ... JO840468 Library and Library 3/12 hpi yes All the genes chosen for expression profiling from the tolerant avocado rootstock infected with Phytophthora cinnamomi showed the highest expression from...
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báo cáo khoa học: " Expression profiling and integrative analysis of the CESA/CSL superfamily in rice" doc
... The total expression of the CSLA genes was highest in plumules (mostly contributed by CSLA1 and 6) and was followed by high expression in radicles (roots) and calli, with the lowest expression ... exhibit different expression patterns Expression divergence of OsCESA/CSL genes in duplication We further observed the expression profiling of the duplicated OsCESA and OsCSL genes The expression of ... same as OsCSLA4 and now has been excluded These updated OsCESA/CSL genes were indentified and characterized in this study We performed expression, co -expression and comparative co -expression analyses...
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Báo cáo y học: "Host gene expression profiling in influenza A virus-infected lung epithelial (A549) cells: a comparative analysis between highly pathogenic and modified H5N1 viruses" ppt
... contributions AKC and ACM conceived and designed the experiments AKC, VCV, SM, RS and SDP performed the experiments AKC, VCV, SM performed data analysis and bioinformatics studies AKC, SM and ACM wrote ... differential expression pattern and were mainly involved in Cytokine-cytokine receptor interaction, Toll like-receptor mediated signaling and p53 signaling pathway [Figure S1 (Additional file 3) and Figure ... highly-pathogenic and RG modified H5N1 infected A549 cell lines (16 h post infection time point) Red arrow indicates expression in highly-pathogenic H5N1 infected A549 cells and Blue arrow indicates expression...
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Báo cáo y học: "Gene expression profiling in circulating endothelial cells from systemic sclerosis patients shows an altered control of apoptosis and angiogenesis that is modified by iloprost infusion" potx
... CECs and EPCs levels between healthy controls and patients affected by SSc with and without ulcers were performed by T-test and Pearson test Correlations between CECs and EPCs number before and ... receptors (TLR2, and 5) in particular TLR3 and TLR5 expression underwent extensive variation in SSc patients after iloprost infusion (F.C -55.82 and -27.49 before treatment to F.C -6.59 and + 2.03 ... (Tree Star, Ashland, OR, USA) was used to analyze data A sequential Boolean gating strategy [28], designed to remove dead cells, platelet aggregates and debris, and to exclude CD45 + and CD3 +/CD19...
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Báo cáo y học: " Gene expression profiling of Hfe-/- liver and duodenum in mouse strains with differing susceptibilities to iron loading: identification of transcriptional regulatory targets of Hfe and potential hemochromatosis modifiers" pdf
... characterized mouse genes, and bioinformatics tools to characterize expression networks in the duodenum and the liver of wild-type control and Hfe-/B6 and D2 mice Results Differential gene expression between ... Cdca7l, Ifit1 and Ifit2, Oasl2, and Scotin), innate antiviral or antimicrobial activity (Defcr4, Ddx58, and Lzp-s), and B and T cell mediated immune response (Mpa2l, Psme1, Trfrsf13b, and Tnfrsf17) ... mice (eight B6 and eight D2) and 16 knockout mice (eight B6 and eight D2) Three mice in each of the four genotype/strain groups were used for genome-wide expression profiling, and five for validation...
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Báo cáo y học: "Genome-wide expression profiling and bioinformatics analysis of diurnally regulated genes in the mouse prefrontal cortex" pps
... hour light and 12 hour dark cycle (LD 12:12) for two weeks We collected tissue samples at four time points, and hours after lights on (ZT3 and ZT9) and and hours after lights off (ZT15 and ZT21), ... diurnally regulated expression patterns in the prefrontal cortex will shed light on the function of prefrontal cortex and provide candidate genes for genetic studies of sleep and psychiatric disorders ... in the sleep:wake cycle and circadian rhythm [12-15] Both the sleep:wake cycle and circadian rhythms are accompanied by diurnally regulated gene expression - the gene expression levels change...
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Báo cáo y học: "Novel insights into the relationships between dendritic cell subsets in human and mouse revealed by genome-wide expression profiling" ppt
... and human BDCA3 cDCs on the one hand, and mouse CD11b and human BDCA1 cDCs on the other hand, clustered together and shared a conserved genetic signature (Figure and Table 6) Thus, although a higher ... intra- and inter-species comparisons, we define the common and specific core genetic programs of DC subsets Results Generation/assembly and validation of the datasets for the gene expression profiling ... populations isolated from mouse and human cathepsin family (Ctsb, Ctsd, Ctsh, Ctss, and Ctsw) as well as Ifi30 and Lamp1 and Lamp2 (see Additional data file for expression values) Thus, it is...
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