detection of putative cancer stem cells of the side population phenotype in human tumor cell cultures
Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps
... This suggests that the use of feeder cells in our cultures of cancer stem cells is likely to be important for maintaining gene expression patterns similar to cancer stem cells in vivo Genome Biology ... PTL induces apoptosis in primitive cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing ... distinct subset of tumor cells [3] that are defined by the expression of CD34+CD38- surface antigens and have been termed leukemic stem cells Similar tumor- initiating cells have also been found in...
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Báo cáo khoa học: " Loratadine dysregulates cell cycle progression and enhances the effect of radiation in human tumor cell lines" pot
... loratadine alone results in Chk1 activation leading to an increase in the percentage of cells in the G2/M phase of the cell cycle Since Page of 12 the G2/M phase of the cell cycle is one of the ... Figure 5B, the percent of cells in G2 did not increase following radiation in loratadinetreated cells In contrast to cells exposed to radiation alone, the loratadine-treated irradiated cells had ... determined by dividing the density of the released DNA band by the density of the total DNA in the lane (the released DNA band plus the unreleased DNA remaining in the well) Page of 12 Cell Cycle...
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Báo cáo y học: " Open Access Quantification of the virus-host interaction in human T lymphotropic virus I infection" pdf
... patients and in the ACs in each of the bins was calculated The test statistic, "number of bins in which the proportion of CD4+ cells expressing Tax was higher in the HAM/TSP patients than the ACs" ... The increase in proviral load due to arate of CTL lysis of The increase in proviral load due to a high rate of Tax expression decreases with increasing rate of CTL lysis of infected cells A theoretical ... also increases infected cell proliferation by upregulating cellular genes involved in proliferation and deregulating cell cycle checkpoints This increase in infected cell proliferation results in...
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Trinucleotide (CAG) repeat polymorphism of the androgen receptor gene in human disease
... resulting in the activation of a number of proteins responsible for the trafficking of nutrients from the Sertoli cells to the germ cells Genetic causes of male infertility In 40-60% of cases, the ... amino acids at the base of the first zinc finger (glycine 577, serine 578 and valine 581) which are essential for the interaction of the AR protein with the hormone response elements (HRE) in the ... modulate proteinprotein interactions, raising the possibility that changes in their length during evolution could result in changes in the strength of protein-protein interactions As a number of studies...
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Báo cáo y học: "Hedgehog overexpression leads to the formation of prostate cancer stem cells with metastatic property irrespective of androgen receptor expression in the mouse model" pps
... prostate cancer cells include malignant cells of luminal, basal or neuroendocrine origin at various proportions and unlike the ChgA- luminal cancer cells, tumor cells of neuroendocrine origin are ... the P63+ basal /stem cells of the pCX-shh-IG-injected prostates, being located in the cell membrane of P63+ basal cells in regions of primary PIN lesions (Figure 3B; arrow in (b) indicated P63 ... [23,24] In this study, we used the Hh overexpression mouse model to elucidate whether the PCSCs arise from P63+ basal /stem cells and to examine whether these cancer cells can maintain stem cell...
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Báo cáo y học: "Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study" pptx
... characteristics of LCNEC and SCLC The preferential expression of the cancer stem cell markers in LCNEC over SCLC suggests that the mechanism of increasing the extent of malignancy in LCNEC differs ... Association for the Study of Lung Cancer International Staging Committee; Participating Institutions: The IASLC Lung Cancer Staging Project: Proposals for the revision of the TNM stage groups in the forthcoming ... from the conventional ones used in this study, consistent with being negative in LCNEC, and with the reported rate for positive staining in SCLC (26 out of 31) [27] The role of AL1A1 in lung cancers...
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Stem CELLS and the FUTURE OF REGENERATIVE MEDICINE pdf
... called the trophoblast, and the cluster of cells inside the sphere is called the inner cell mass At this stage, there are about 70 trophoblast cells and about 30 cells in the inner cell mass The cells ... system cells, insulin-producing cells of the pancreas, bone cells, hematopoietic cells, yolk sac, endothelial cells, primitive endodermal cells, and smooth and striated muscle cells, including cardiomyocytes—heart ... understanding the capabilities of adult stem cells is the relationship of the cellular environment to the concept of plasticity in adult stem cells Markus Grompe showed that HSCs and pancreatic stem cells...
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Báo cáo khoa học: "Cell death by the quinoxaline dioxide DCQ in human colon cancer cells is enhanced under hypoxia and is independent of p53 and p21" pdf
... protein in the human colon carcinoma cell line T-84, and in EMT6 mouse mammary carcinoma cells and Lewis Lung Carcinoma (LLC) cells [4,8] We also showed that DCQ inhibited cell proliferation and induced ... expression in p53+/+ HCT116 cells SSB and DSB induced by DCQ in p53+/+ cell line (A) Examples of comets induced by DCQ in cells subjected to the alkaline comet assay Cells treated with DCQ for hrs in ... protein also increased in all cell lines except in p53+/+ cells exposed to normoxia (Figure 6A) Exposure of the p53+/+ cells to or 10 μM DCQ gradually increased the level of caspase-2, and the...
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Báo cáo y học: "Hypoxic conditions increase hypoxia-inducible transcription factor 2α and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients" pps
... Research & Therapy Vol No Khan et al practical access, cells from the IPFP are of interest as a potential source of cells for the repair of focal cartilage defects in the knee [5] In previous ... monolayer-cultured IPFP cells; this remained high in the chondrogenic cultures and there was no downregulation of its expression under hypoxic conditions The chondrogenic response of IPFP cells in cell aggregate ... stem cells, and although they stained poorly for STRO-1 and for LNGFR, the expression of these markers on bone marrow-derived stem cells is reported to decline with culture [22-25] In preliminary...
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báo cáo khoa học: " MicroRNAs involved in neoplastic transformation of liver cancer stem cells" pot
... this is the first report demonstrating the existence of SP cells in both fetal liver cells and in primary rodent HCC cancer cells induced by chemical carcinogens Since the HCC cancer cells and ... for cellular heterogeneity of cancer due to aberrant differentiation According to the hierarchical model of cancer, the origin of the cancer stem cells may be long-lived somatic stem cells Therefore, ... differentiation of normal stem cells strongly suggest that miRNA may be involved in the transformation of normal stem cells into cancer stem cells Therefore, screening for differences in miRNA expression...
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báo cáo khoa học: " Microarray-based analysis of microRNA expression in breast cancer stem cells" doc
... be expressed in human embryonic stem cells and other stem cells, indicating that these miRNAs may play a constitutive role in maintaining the biological characteristics of stem cells [40,41] ... expressed in MCF-7 cells, may be one of the causes of high expression of the drug-metabolising enzyme CYP1B1 in cancerous tissues [14] Finally, as a tumor suppressor in breast cancer cells, miR-17-5p ... breast cancer cells with stem/ progenitor cell properties from a breast cancer cell line, and Huang [17] screened side population (SP) cells from a breast cancer cell line Here, we investigated the...
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Báo cáo y học: "Population genetics of cancer cell clones: possible implications of cancer stem cells" pptx
... proportion of 1% to a relative proportion of 99% given a range of differences in the intrinsic rate of increase of the two clones rA - = the intrinsic rate of increase of clone A minus the intrinsic ... generations, P0 = the starting proportion of the two clones, Pt = the final proportion of the two clones, rA = the intrinsic rate of increase of clone A and = the intrinsic rate of increase of clone a ... is the time interval, r is the intrinsic rate of increase and x is the starting population size Another way to think of r is as the ‘compound interest’ on the increase in population size Consider...
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Transplantation of mesenchymal stem cells for the treatment of parkinsons disease in a mouse model
... microglial cells in the SNc of MPTP-treated mice, real-time RT-PCR did not find mRNA expression of MBL in the brain parenchyma, suggesting the infiltration of MBL from the circulation into the brain ... from the blood into the brain parenchyma where they may then interact with microglia In addition to the presence of these inflammatory cytokines, other inflammatory mediators or ongoing inflammatory ... to suppress the function of a broad range of immune cells, including T cells, B cells, NK cells and antigen-presenting cells (Fig 4) and thus may have therapeutic effect in autoimmune diseases...
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The NR4A orphan nuclear receptors are target genes of the novel drug c1 in cancer cells and potential mediators of drug induced apoptosis
... separates the aminoterminal DNA-binding domain of the enzyme from the carboxy-terminal catalytic domain resulting in the loss of normal PARP1 function Although the role of PARP in apoptosis remains ... essential for the development and maintenance of midbrain dopaminergic neurons, the cells that degenerate during Parkinson’s disease, by promoting the transcription of genes involved in dopaminergic ... than 97% homology in their DNA binding domains They also display 37 – 53% homology in the N terminal transactivation domains and 53 - 77% in the C terminal ligand binding domains (Fu et al., 2007)...
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Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
... stem cells Landmark discoveries of the young field of human stem cell science were the isolation and culture of inner cell mass from human blastocysts by Bongso in 1994 and in the derivation of the ... source of cells and the potential to derive the cell type of interest together with the possibility to enrich for genetic modifications during the dividing stem cell state 1.1.1 Human pluripotent stem ... Application of adenosine-releasing lt-NES cells in mouse models of epilepsy 75 4.4.3 Diagonal grafting of lt-NES cells results in distribution throughout the hippocampus in a kindling model of epilepsy...
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Báo cáo khoa học: Transient increase of the labile iron pool in HepG2 cells by intravenous iron preparations Brigitte Sturm, Hans Goldenberg and Barbara Scheiber-Mojdehkar doc
... to cells others than the reticuloendothelial cells, their effect on intracellular iron metabolism and indirectly on the labile iron pool of the human hepatoma cells HepG2 [8] The polymers increase ... LIP The increase in ferritin by the iron preparations showed a pattern of behavior similar to the increase of the LIP The more iron appeared in the LIP the faster the synthesis of ferritin took ... decreased Therefore we assessed the time course of the molar ratio of iron and ferritin in HepG2 cells following IVI exposure for 0–24 h The decrease in the iron content of ferritin paralleled the increase...
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Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx
... RV1 rhadinoviruses A DNA sample from the KSHV infected BCBL-1 cell line [24] containing approximately × 106 copies of the KSHV genome and a sample containing 109 copies of a PCR product of the ORF59/60 ... coefficients cells (Table 2) However, in four of the six positive macaques, the RV-2 assay result was low and outside the linear range of the assay Discussion The novel ORF 59/60 intergenic regions of ... levels of 320 and 880 genomes per 106 cells In the other 28 animals, the RRV load was below the level of detection While RRV was detected more frequently in SIV-infected macaques in this study, the...
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báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot
... RV1 rhadinoviruses A DNA sample from the KSHV infected BCBL-1 cell line [24] containing approximately × 106 copies of the KSHV genome and a sample containing 109 copies of a PCR product of the ORF59/60 ... coefficients cells (Table 2) However, in four of the six positive macaques, the RV-2 assay result was low and outside the linear range of the assay Discussion The novel ORF 59/60 intergenic regions of ... levels of 320 and 880 genomes per 106 cells In the other 28 animals, the RRV load was below the level of detection While RRV was detected more frequently in SIV-infected macaques in this study, the...
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Báo cáo khoa học: "Detection of swine hepatitis E virus in the porcine hepatic lesion in Jeju Island" doc
... ygolohtapotsih rof nilamrof dereffub lartuen %01 ni dexif saw trap gniniamer eht elihw ,)RCP( noitcaer niahc esaremylop rof Co07− ta nezorf saw revil hcae fo driht-enO ysporcen ta sgip morf detcelloc ... nI yrassecen si sgip ronod ni VEH eniws rof ygolonhcet gnineercs etarucca dna evitisnes a fo tnempoleved eht ,eroferehT noitatnalp -snartonex ni esu rof snoitatimil evah sgip detcefni-VEH eniws ... )'3-GGTCGTCGCTGTCTACTGCACTCG-'5( 2F remirp drawrof eht htiw demrofrep ,RCP dnuor-dnoceS )'3-CGTT AGTTCCGACCGCGAGACATC-'5( 1R remirp esrever eht dna )'3-CTCAGTTGTAGTCCATGTCCTCGA-'5( 1F remirp drawrof eht htiw )pb( sriap...
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