detection of hif 1 by western blotting
STUDY ON EPIDEMIOLOGICAL FEATURES, APPLICATION OF DIAGNOSTIC KIT FOR DETECTION OF TRYPANOSOMIASIS CAUSED BY TRYPANOSOMA EVANSI IN CATTLE AND BUFFALOES IN A FEW NORTHERN MOUNTAINOUS PROVINCES AND RECOMMENDATION FOR PREVENTIVE AND TREATMENT MEASURES
... 0 /10 3 /10 3 /10 3 /10 2/3 3 /10 4 /10 7 /10 7 /10 7 /10 0 /10 5 /10 10 /10 10 /10 10 /10 0 /10 0 /10 0 /10 0 /10 0 /10 0 /10 3 /10 9 /10 10 /10 10 /10 10 /10 0 /10 0 /10 0 /10 0 /10 0 /10 0 /10 Table 3.29 shows that at dose ... 1/ 10 2 /10 1/ 10 1/ 10 0 /1 2 /10 5 /10 5 /10 7 /10 9 /10 3 /10 4 /10 6 /10 6 /10 6 /10 2/6 1/ 10 1/ 10 3 /10 4 /10 4 /10 5 /10 7 /10 9 /10 9 /10 7 /10 1/ 10 1/ 10 1/ 10 1/ 10 3 /10 1/ 7 Table 3.28 shows that at dose lower ... drugs was used 0 0 8 /10 10 /10 10 /10 10 /10 - 6 /10 6 /10 9 /10 6 /10 2/6 0 1/ 10 4 /10 10 /10 10 /10 9 /10 7 /10 0/7 0 1/ 10 3 /10 10 /10 10 /10 9 /10 7 /10 0 1/ 10 3 /10 0/7 Table 3.30 shows that at dose lower than...
Ngày tải lên: 28/04/2014, 13:08
Báo cáo y học: "Catabolic stress induces expression of hypoxia-inducible factor (HIF)-1α in articular chondrocytes: involvement of HIF-1α in the pathogenesis of osteoarthritis" ppt
... Circ Res 20 01, 88 :12 47 -12 53 44 Jewell UR, Kvietikova I, Scheid A, Bauer C, Wenger RH, Gassmann M: Induction of HIF- 1a in response to hypoxia is instantaneous FASEB J 20 01, 15 :13 12 -13 14 R 914 ... face of catabolic changes in articular cartilage Many molecular aspects of HIF- 1 signaling should be also studied to further clarify the role of HIF- 1 in the pathogenesis of OA References 10 11 12 ... Rheum 2002, 46:2 911 -2 916 Pfander D, Cramer T, Schipani E, Johnson RS: HIF- 1alpha controls extracellular matrix synthesis by epiphyseal chondrocytes J Cell Sci 2003, 11 6 :18 19 -18 26 Hall JL, Matter...
Ngày tải lên: 09/08/2014, 06:23
Báo cáo y học: "α The role of HIF-1α in maintaining cartilage homeostasis and during the pathogenesis of osteoarthritis" pot
... 10 11 12 13 14 15 Yudoh K, Nakamura H, Masuko-Hongo K, Kato T, Nishioka K: Catabolic stress induces expression of hypoxia-inducible factor (HIF) -1a in articular chondrocytes: involvement of HIF1 a ... Arthritis Rheum 20 01, 44 :10 82 -10 88 Pfander D, Cramer T, Schipani E, Johnson RS: HIF- 1alpha controls extracellular matrix synthesis by epiphyseal chondrocytes J Cell Sci 2003, 11 6 :18 19 -18 26 Lin C, McGough ... reported that mechanical overload of bovine cartilage discs leads to an increased expression of VEGF-A via HIF- 1 In conclusion, the α-subunit of HIF- 1 is stabilized and HIF- 1 activity is significantly...
Ngày tải lên: 09/08/2014, 07:20
Báo cáo y học: " Preferential binding of HIF-1 to transcriptionally active loci determines cell-type specific response to hypoxia" docx
... Genome Biology 2009, 10 :R 113 http://genomebiology.com/2009 /10 /10 /R 113 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Genome Biology 2009, tion Genes Dev 19 89, 3: 612 - 619 Perini G, Diolaiti ... Biology 2009, 10 :R 113 http://genomebiology.com/2009 /10 /10 /R 113 U87 10 27 766 209 475 205 14 7 967 97 HepG2 3 01 1285 246 MB2 31 276 14 6 HepG2 3 51 MB2 31 GAPDH MLL5 PPME1 (d) 10 00.0 p =1. 1e- 41 100% 20 47 ... All HIF1 bound HIF1 bound p
Ngày tải lên: 09/08/2014, 20:20
Báo cáo y học: " Detection of HIV-1 RNA/DNA and CD4 mRNA in feces and urine from chronic HIV-1 infected subjects with and without anti-retroviral therapy" pptx
... lamina propria J Immunol 19 92, 14 9:2 816 -2822 Page 10 of 11 (page number not for citation purposes) AIDS Research and Therapy 2009, 6:20 10 11 12 13 14 15 16 17 18 19 20 21 Brenchley JM, Schacker TW, ... XX186 9/24/2008 N/A 839 XX148 9 /17 /2008 N/A 724 XX0 21 8 /19 /2008 N/A 845 XX280 8/26/2008 10 03 923 XX495 8 /16 /2008 354 71 238 XX326 8/23/2008 2 014 9 392 XX286 9 /17 /2008 2974 320 XX 119 8/26/2008 18 2 31 ... HIV -1 Negative N = 10 Sample Date Viral Load (copies/ml) CD4/mm3 642 N/A 13 17 XX163 8 /19 /2008 N/A 899 XX983 8 /19 /2008 N/A 11 53 XX2 71 9/5/2008 N/A 828 XX003 9 /16 /2008 N/A 547 XX744 9/25/2008 N/A 15 20...
Ngày tải lên: 10/08/2014, 05:21
Báo cáo khoa học: " A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes" pps
... by Sigma-Proligo, Singapore Table Primers and probe used in this study Name Sequencea Nucleotideb Sense 5’-TTGGTTACCATGCAAACAAYT-3’ 91- 111 Antisense 5’-TRTCTTGGGCRTGTGTAACA-3 15 2 -17 1 Probe 11 9 -14 3 ... virus in eastern Asia Nature 2004, 430(6996):209- 213 WHO: Evolution of H5N1 avian influenza viruses in Asia Emerg Infect Dis 2005, 11 (10 ) :15 15 -15 21 Smith GJ, Naipospos TS, Nguyen TD, de Jong MD, ... TL, Lim WW: Influenza A H5N1 detection Emerg Infect Dis 2005, 11 (8) :13 03 -13 05 18 Lu YY, Yan JY, Feng Y, Xu CP, Shi W, Mao HY: Rapid detection of H5 avian influenza virus by TaqMan-MGB real-time...
Ngày tải lên: 12/08/2014, 04:21
Báo cáo y học: "Extensive complement-dependent enhancement of HIV-1 by autologous non-neutralising antibodies at early stages of infection" pdf
... 759 IC90 < 10 < 10 < 10 < 10 < 10 45 74 19 2 < 10 10 IC50 < 10 < 10 < 10 < 10 < 10 < 10 10 Patient MM38 DFOS 29 IC90 < 10 < 10 < 10 < 10 < 10 36 51 58 93 IC50 < 10 < 10 < 10 < 10 < 10 Patient MM42 ... 320 12 80 12 80 12 80 2560 12 80 38 66 94 18 5 76 53 83 10 9 11 1 299 Patient MM28 DFOS IC90 20 34 62 93 19 8 < 10 < 10 < 10 < 10 < 10 405 < 10 503 < 10 782 < 10 950 < 10 IC50 < 10 < 10 < 10 < 10 < 10 ... < 10 < 10 < 10 Patient MM33 DFOS 26 2 01 523 6 21 719 IC90 < 10 < 10 < 10 < 10 < 10 33 69 96 < 10 < 10 < 10 IC50 < 10 < 10 < 10 < 10 < 10 < 10 < 10 < 10 Patient MM34 DFOS 32 443 607 759 IC90 < 10 ...
Ngày tải lên: 13/08/2014, 01:20
Báo cáo y học: " Trans-inhibition of HIV-1 by a long hairpin RNA expressed within the viral genome" pps
... days HIV + AS19 M 10 :1 1 :1 1 :10 - HIV - AS19 10 00 800 600 - C M 10 00 800 - HIV R1 AS19 12 16 19 23 12 16 19 23 12 16 19 23 days HIV + R1 AS19 + R1 - HIV - R1 - AS19 10 21 42 52 62 10 21 42 52 62 ... LAI-lhNef AS44 AS15 HIV HIV-lhNef AS44 AS19 AS15AS19 AS 11 HIV HIV- AS44 AS19 AS15 AS 11 lhNef AS 11 AS6 AS6 AS6 R1 B trans-inhibition of HIV -1 CA-p24/RL (%) 360 360 300 300 240 240 18 0 18 0 12 0 12 0 60 60 ... SupT1 winner HIV/AS44 HIV/AS19 HIV/AS15 HIV/AS 11 HIV/AS6 HIV/R1 AS19/R1 HIV (4)b HIV (58) HIV (23) HIV (23) HIV (11 ) HIV (52) AS19/R1 (>62) HIV (4) AS19 (12 ) AS15 (23) AS 11 (33) AS6 (16 ) HIV/R1...
Ngày tải lên: 13/08/2014, 09:20
ICA based EEG energy spectrum for detection of negative emotion by EEG
... EXPERIMENTAL DESIGN 31 4 .1 4 .1. 1 4 .1. 2 4 .1. 3 4.2 4.3 4.3 .1 4.3.2 4.3.3 4.3.4 4.4 4.4 .1 4.4.2 BIOLOGICAL BASIS OF EMOTION . 31 Emotion Loop 32 Function of limbic system ... -500 500 O1 300 -10 00 500 Pz 200 -500 10 00 P4 10 0 -500 (a) twelve seconds of anxiety emotion state EEG raw data 22 ICA-based EEG Energy Spectrum 10 C1 -10 C2 500 600 700 800 900 10 00 10 0 200 300 ... value of SVM during optimization 71 vii List of Tables LIST OF TABLES Table 4 .1 Function of components of limbic system 34 Table 4.2 The conductivity (S/m) of tissues below 10 0...
Ngày tải lên: 22/10/2015, 21:19
Báo cáo khoa học: "Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe" pps
... 0 .11 7. 71 ± 0 .12 8 .13 ± 0.02 8.72 ± 0 .13 8.4 ± 0 .19 8 .14 ± 0 .16 8.02 ± 0.05 7.97 ± 0.04 8.75 ± 0.20 7.94 ± 0 .11 8.32 ± 0 .16 8.00 ± 0 .11 7.95 ± 0.09 8.23 ± 0 .10 8. 41 ± 0 .19 1d Table Mean AHV -1 ... 29.63 0. 21 0.70 1. 00E+04 33.07 0. 31 0.92 1. 00E+03 36.33 0. 31 0.84 1. 00E+02 39.50 0 .17 0.44 1. 00E+ 01 42.67 0.32 0.75 1. 00E+08 19 .70 0.28 1. 41 1.00E+07 1. 00E+06 23.09 26. 31 0.47 0.32 2.03 1. 23 1. 00E+05 ... 7.33 ± 0 .19 6.78 ± 0.05 7.70 ± 0 .18 6.64 ± 0 .11 7. 61 ± 0 .19 7 .14 ± 0 .18 7.23 ± 0 .12 7.24 ± 0.07 7.76 ± 0 .14 7.39 ± 0 .15 7.84 ± 0.08 7 .11 ± 0 .15 7.30 ± 0 .16 6.84 ± 0.06 7.53 ± 0 .12 7.29 ± 0 .11 7.87...
Ngày tải lên: 12/08/2014, 04:21
Tài liệu Báo cáo khoa học: Rac upregulates tissue inhibitor of metalloproteinase-1 expression by redox-dependent activation of extracellular signal-regulated kinase signaling pptx
... the effect of C 119 9-Tiam1 In addition, the effects of MEK1,2 inhibitors and catalase on C 119 9Tiam1-induced TIMP -1 secretion in quantitative TIMP -1 immunoassays were verified by immunoblotting (data ... Miki T & Gutkind JS (19 95) The small GTP- 4766 16 17 18 19 20 21 22 23 binding proteins Rac1 and Cdc42 regulate the activity of the JNK ⁄ SAPK signaling pathway Cell 81, 11 37– 11 46 Bagrodia S, Taylor ... overexpression of C 119 9-Tiam1 strongly activated Rac, but neither C 119 9-Tiam1 nor V12-Rac1 affected Rho activity (Fig 3D) This suggests that Rac-induced upregulation of TIMP -1 is neither 4758 mediated by...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc
... 2.30 1. 50 4.87 1. 75 4.27 2 .12 1. 70 1. 24 2.69 2.00 4.36 5.02 1. 59 2.45 · · · · · · · · · · · · · · · a )4 10 10 )4 10 )4 10 )4 10 )3 10 )5 10 )6 10 )6 10 )5 10 )4 10 )4 10 )5 10 )6 10 )5 10 )5 c d Type of inhibition ... of the Dixon plot and/or Lineweaver–Burk plot Compound Tat (1 9) Tat (1 9)a Trp1-Tat (1 9) Gly3-Tat (1 9) Ile3-Tat (1 9) Lys2-Tat (1 9) Trp2-Tat (1 9) Trp2-Tat (1 9)* Met-Trp1-G-CSF (1 8) Met-IL-2 (1 12 ) ... )5 h, 1. 5 · 10 M; n, · 10 M; ,, · 10 M; e, · 10 )5 M; d, · 10 )5 M) represented as a Dixon plot (1/ v vs [I]) (B) Lineweaver– Burk plot (1/ v vs 1/ [S], [I] ¼ s, · 10 )4 M; h, · 10 )4 M; n, · 10 )4 M;...
Ngày tải lên: 20/02/2014, 11:20
Tài liệu Báo cáo khoa học: "Detection of Japanese Homophone Errors by a Decision List Including a Written Word as a Default Evidence" docx
... { t~-~-=, ~=-~ } \ e 0.7 0.6 o 0.5 0,4 0.3 % o.2 LR0 LR1 10 62 11 04 11 20 11 34 10 07 9 21 760 811 799 760 697 695 844 6 71 667 622 424 9 21 319 788 469 665 255 397 homophone word to another homophone ... number of problems detected as errors by DL1 is given by Eq.4, and the number of real errors in these detections is TGpg Therefore, the precision P1 of DL1 is as follows: p1 = Pg by a is 1, 6 31, and ... the recall of DL1 Because a of DL1 is the same as that of DL0, the number of problems detected as errors by a is given by Eq.4 In the case of DL1, problems judged by ~ of DL0 are judged by the written...
Ngày tải lên: 22/02/2014, 03:20
Báo cáo khoa học: Suppression of a cold-sensitive mutant initiation factor 1 by alterations in the 23S rRNA maturation region pdf
... cold-sensitive IF1 10 11 12 13 14 15 References Laursen BS, Sorensen HP, Mortensen KK & SperlingPetersen HU (2005) Initiation of protein synthesis in bacteria Microbiol Mol Biol Rev 69, 10 1 12 3 Pon CL, ... form of the processing stem of 23S rRNA (helix 1) is not proximal to the subunit interface of 50S, suggesting that direct contacts between IF1 and FEBS Journal 278 (2 011 ) 17 45 17 56 ª 2 011 The ... 278 (2 011 ) 17 45 17 56 ª 2 011 The Authors Journal compilation ª 2 011 FEBS J M Belotserkovsky et al Processing stem mutations suppress cold-sensitive IF1 Fig A model of possible mechanism of suppression...
Ngày tải lên: 06/03/2014, 00:21
Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
... pg per oocyte): m2R: 15 00; GIRK1F137S: 37.5; GIRK1F137SS385C: 37.5; GIRK1F137SS401C: 37.5; GIRK1F137ST407C: 37.5; GIRK1F137SS385CS401CT407C: 15 0 Oocytes were kept in NDE at 19 °C for 3–5 days after ... mmolÆL )1 KCl, mmolÆL )1 NaCl, mmolÆL )1 MgCl2, mmolÆL )1 CaCl2, mmolÆL )1 Hepes buffered with KOH to pH 7.4; Glutathione buffer: 12 0 mmolÆL )1 NaCl; 0.05% Tween-20; 10 0 mmolÆL )1 Tris; 15 mmolÆL )1 glutathione ... mutation (GIRK1F137SS385C: 0. 21 ± 0.04; 0.26 ± 0.05; GIRK1F137ST407C: GIRK1F137SS401C: Fig Effect of cAMP injections on homooligomeric GIRK1 wildtype and mutated channels (A) Effect of cAMP injection...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc
... 26, 817 5– 818 2 31 Coleman DE, Berghuis AM, Lee E, Linder ME, Gilman AG & Sprang SR (19 94) Structures of active conformations of Gia1 and the mechanism of GTP hydrolysis Science 265, 14 05 14 12 32 ... 15 8, 643–655 19 Matsuki M, Kunitomo H & Iino Y (2006) Goalpha regulates olfactory adaptation by antagonizing GqalphaDAG signaling in Caenorhabditis elegans Proc Natl Acad Sci USA 10 3, 11 12 11 17 ... Biomol Screen 10 , 12 7 13 6 Gether U & Kobilka BK (19 98) G protein-coupled receptors J Biol Chem 273, 17 979 17 982 Lefkowitz RJ (19 89) G protein-coupled receptors J Biol Chem 273, 18 677 18 680 Perfus-Barbeoch...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Selective inhibition of ADAMTS-1, -4 and -5 by catechin gallate esters ppt
... Mono-Q 19 0 1. 1 0.20 11 0 82 44 0.60 75 220 (10 0) 75 40 (1) 12 5 367 rhADAMTS-5 Medium Heparin F-F Mono-Q 11 0 2.8 0 .10 720 410 12 0 (10 0) 57 17 (1) 23 18 5 Results Purification and characterization of ... 200 17 0 13 0 rhADAMTS-5 280 200 17 0 13 0 10 0 N-terminal sequence Yield (pmol) V1EVSEPDN V1EVSEPXN G1481RXTXD G1667LGSVEL V1EVSEPD A374RGSVIL A1772GEGPSGI V1EVSE G1481RXTXD L1872GQRPXV G1481RXTXD ... V1EVSEPDN V1EVSEPDN A374XGS G1481RGTXD V1EVSEP G1667LGSVEL A374RGSV V1EVSEP A1772GEGPSGI 13 12 20 A374XGSVIL V1EVSEPDN G1481RGTIDI A374RGSVXL G1667LGSVEL A1772GEGPXGI L1872GQRPPV 9 cleavage of...
Ngày tải lên: 08/03/2014, 02:20