detecting dna methylation using the methylated cpg island amplification and microarray technique
báo cáo khoa học: " Ovarian cryopreservation after laparoscopic ovariectomy using the Endo-GIA stapling device and LAPRO-clip absorbable ligating clip in a woman: a case report" ppt
... sufficiently in the work IR, XD and MG performed surgical procedure and analyzed and interpreted the patient data regarding the surgical management JL and AM performed the ovarian cryopreservation and were ... Cutting the mesovarium using the Endo-GIA stapling device References American Society for Reproductive Medicine, The Practice Committee of the American Society for Reproductive Medicine and the Practice ... tissue damage on the outcome of ovarian tissue harvesting and reimplantation Further studies should assess ovarian tissue damage and the results of ovarian cryopreservation according to the ovarian...
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A cross-cultural study of using the Sandwich feedback by Vietnamese and American teachers and its effects on students’ uptake
... and the suggestion for further researches 44 4.3 Conclusion REFERENCE QUESTIONNAIRE 45 46 References Abbas, A M (2010) The concept of pragmatic politeness with reference to standard English and ... "The Thorn Birds" University of Languages and International Studies, Hanoi Pennington, R (2011) The Feedback Sandwich Resource International, Seamon's, R (2001) The Routledge Companion of Aesthetics ... 2010) The relationship between Language and Culture Retrieved May 17th, 2012, from http://www.lexiophiles.com/topic/therelationship-between-language -and- culturehttp://www.lexiophiles.com/topic/therelationship-between-language -and- culture...
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INVESTIGATION OF a LYSIMETER USING THE SIMULATION TOOL siwapro DSS AND ADAPTATION OF THIS PROGRAM TO VIETNAMESE REQUIREMENTS
... A and B, equation (10) can be written by equation (11): (11) PA and PB are the partial vapor pressures of the components A and B XA, XB are the mole fraction of the component A and B So, XA and ... of the substances, which is called the solvent, and may itself be a mixture, is treated differently than the other substances, which are called solutes If the sum of the mole fractions of the ... expressed by the log-linear model 21 log S=log Sw+σf where (2) S and Sw are the solubility in the co solvent–water mixture and water, respectively f the fraction of the co solvent σ the solubilization...
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![Tài liệu Báo cáo khoa học: The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases pptx](https://media.store123doc.com/images/document/14/br/gc/small_Y97X8XlBli.jpg)
Tài liệu Báo cáo khoa học: The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases pptx
... compared with the levels of DNA methylation, and thus the effect of the absolute configurations and conformations of the adducts on DNA methylation was not evaluated More recently, the effect of ... 32P-labeled DNA (R) is the ratio of the fraction of bound 32 P-labeled DNA in the presence of the competitor DNA (cpmbound ⁄ cpmtotal) to the fraction of bound 32P-labeled DNA in the absence of the competitor ... (+)cis-B[a]P-N2-dG on the 5¢-side of the target dC residue The kcat value for the hemimethylated duplex GCX+ ⁄ CGM was about the same as that for the GCG ⁄ CGM duplex The kcat values determined for the unmethylated...
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Báo cáo khoa học: Auto-methylation of the mouse DNA-(cytosine C5)-methyltransferase Dnmt3a at its active site cysteine residue pot
... by the action of the Dnmt3a and Dnmt3b de novo DNA methyltransferases (MTases) Once established, the methylation pattern is further maintained during each DNA replication and cell division by the ... standard auto -methylation reaction Whereas Dnmt3L increases the level of auto -methylation of Dnmt3a-C, the methylation of the dsDNA substrate is efficiently competing with the Dnmt3a-C auto -methylation ... double-stranded DNA substrate abolished the tritium incorporation into the MTase but at the same time the DNA got efficiently methylated This result illustrates that after binding both substrates (DNA and...
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Báo cáo khoa học: CpG methylation of the CENP-B box reduces human CENP-B binding pptx
... unmethylated CENP-B box DNA (A) The 21-mer CENP-B box DNA and the 21-mer nonspecific DNA (DnaA box DNA [30]), used in this study The methylated cytosine residues in the methylated CENP-B box DNA ... the unmethylated and methylated CENP-B box DNAs The 32P-labeled unmethylated CENP-B box DNA (1 lM) or the 32P-labeled methylated CENPB box DNA (1 lM) was mixed with the indicated amounts of the ... with the unmethylated CENP-B box DNA, rather than the methylated CENP-B box DNA Y Tanaka et al The CENP-B binding affinity to the methylated CENP-B box DNA is similar to the level of nonspecific DNA...
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báo cáo hóa học:"Detecting exacerbations using the Clinical COPD Questionnaire" docx
... when they were present that day and not part of their normal symptom status Patients were contacted by telephone by the investigators after the first days to review their compliance and understanding ... exacerbations in the analysis demands accounting for dependency on the patient level and the inherent correlation of paired data In our analysis the impact of multipleevents is marginal since only of the ... Utrecht, The Netherlands Authors’ contributions All co-authors have read and approved the final manuscript JCAT - leading of development of the study conceptualisation, design, refining of protocol and...
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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf
... enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled ... pre-treatment, and overnight hybridization The practicality of digoxigenin-labeled probe is better than those of biotin-labeled probe in the hybridization assay for the detection of CHV These results ... digoxigenin-labeled probe and streptavidin conjugated with alkaline phosphatase (Roche) for biotinlabeled probe respectively, and then colorized with NBT/ BCIP (Roche) The results of ISH were presented...
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Báo cáo thú y: " DNA methylation patterns detected by the Amplified Methylation Polymorphism Polymerase Chain Reaction (AMP PCR) technique among various cell types of bulls" pot
... maintain their functions, and the rest are suppressed by DNA methylation or other gene regulation processes [21] On the other hand, somatic cells possessing the ability to change their morphology and ... used in the experiment DH and BC contributed in the experimental designs and techniques MO and MT provided the concept of the experiment and helped to draft the manuscript All authors read and approved ... markers: R marker between leukocytes and fibroblasts, S marker between leukocytes and the others and D marker between fibroblasts and the others The summary of the individual variations mentioned...
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THE ROLE OF DNA METHYLATION IN REGULATING LHX3 GENE EXPRESSION
... microenvironment and the developmental transcription factor, LHX3 17 3.3 DNA methylation DNA methylation represents another level of transcriptional control and regulation in the cell DNA methyltransferases ... Complementary DNA (cDNA) was synthesized from μg of total RNA using High Capacity Reverse Transcription kit (Applied Biosystems, Grand Island, NY) according to the manufacturer’s protocol The cDNA was ... 11A, B) The CpG sites at +12 and +17 of the Lhx3b 39 promoter were not as affected by the treatment, with 57% and 50% of the clones methylated at these positions, respectively Trichostatin A and...
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AN1016 detecting small capacitive sensors using the MCP6291 and PIC16F690 devices
... worth the effort The dominant error sources are covered in this section They will be covered in the same sequence they propagate through the circuit and algorithm Their impact on RH accuracy, and ... contribute to the current imbalance The common mode voltage setting resistors (RCM1 and RCM2) and the op amp (U1) dominate the VCM errors If RCM1 is 1% low and RCM2 is 1% high, then the relative ... (INTRVL) to the correct row of the table The bottom bits of CSN (CSN – ACSN, interpreted as integers between and 15) are the input to the linear interpolation formula using the coefficients for the current...
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Expert Service Oriented Architecture in C Sharp Using the Web Services Enhancements
... platforms such as J2EE and IBM WebSphere I have full respect for these platforms and for the efforts of the developers and architects who use them These guys and girls “get it,” and they have been doing ... forms To some it is the freedom to recompile their C++ code on another compiler Others think of freedom as the ability to see and modify the source code of underlying libraries The portability of ... comfort zone, and to help you rethink the technology and think of the bigger picture that is SOA I devote the first part of this book to clearing up the misconceptions And I devote the second part...
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USING THE ANALYTIC HIERARCHY PROCESS APPROACH FOR ASSESSMENT OF THE STRENGTH OF UNIVERSITY-INDUSTRY-GRI COOPERATION IN VIETNAM
... to the others For example, the flow from University to Industry and vice versa Therefore, these flows become the main factor in the individual model and are considered as the third level The ... of the report contains the most relevant matters on the research and details input and output of analysis and supportive information are given in the appendix part The first chapter gives the ... introduction and the problem description The next chapter deals with the literature review related to the problem and tool used in the research In the third chapter, detail methodology of the work...
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PREDICTION OF ATRAZINE FATE IN RIPARIAN BUFFER STRIPS SOILS USING THE ROOT ZONE WATER QUALITY MODEL
... (Simpkins and Schultz, 1993) was used as the basis for model inputs and simulation The Bear Creek watershed is situated within the Des Moines Lobe landscape, the depositional remnant of the late ... 1995) The five-year-old switchgrass buffer strips (age in 1998) and cropped areas (corn-soybean rotation) are located on the Strum Farm in the southern half of the Bear Creek channel system The ... channel system The elevation of the site is 318 m The aspect from true north is 200 degrees and the latitude is 42 degrees The slope of the field is 5.71 degrees These values were estimated by...
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Removal of arsenic from synthetic groundwater by adsorption using the combination of laterite and ironmodified activated carbon
... and atmospheric condition The samples were taken then acidified and analyzed for residual arsenic concentration A mixture of 70% As[III] and 30% As[V] was used in all of the experiments and the ... in the values of the regression coefficient r2 Therefore, adsorption process of arsenic onto LA and AC-Fe may be described by Langmuir isotherm and the adsorption capacities were 0.48mg/g and ... for LA and AC-Fe are 0.48mg/g and 1.18mg/g, respectively) However, it is also possible to realize that the longer the portion of AC-Fe, the higher the adsorbent cost Therefore, optimizing the AC-Fe...
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Using the Shell
... previous ls command, replacing CF with l Another way to edit the command history is using the fc command With fc, you open the chosen command from history using the vi editor The edited command runs ... bash` The first command line in the preceding example finds the full path of the ps command and finds the package that contains that ps command The second command line finds the full path to the ... only the path to the man page (the second element on the line) Using backticks, you can execute one section of a command line first and feed the output of that command to the rest of the command...
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Using the vi Editor
... list the vi editor’s editing and positioning commands, which are used to make changes The vi editor is case sensitive, so use the specified case when using the editing and positioning commands The ... text at the end of the line Insert text before the cursor Insert text at the beginning of the line Open a new line below the cursor Open a new line above the cursor Positioning Commands The following ... command U Undo all changes to current line :u Undo previous last-line command Copying and Pasting Text To copy and paste text, use the following options: Table 9-6 Copy and Paste Commands Command...
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AJAX-Style Mapping Using the Virtual Earth SDK
... MAPPING USING THE VIRTUAL EARTH SDK Barnes and Noble locations in the Chicago area along with their addresses, phone numbers, and other information The search results appear in the left pane, and their ... top The PanXY JavaScript function you saw in the preceding code segment reads the X and Y coordinates from the text boxes on the page and pans the map accordingly using the Pan method of the ... The VEMap control takes in the ID of the tag as the host container in its constructor VEMap is at the heart of the VE API, and as the core component, it handles most of the functionality...
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Using the ASP.NET AJAX Control Toolkit (Part 1)
... of the regular button, and set the text for the ConfirmText property Lastly, create a Label control, and in the event handler for the button, set the label’s text to a message indicating the ... ID of the control responsible for expanding the panel ExpandDirection Direction of expansion of the panel (horizontal/vertical) ExpandedImage Displayed image when the panel is expanded ExpandedSize ... CollapsedImage="~/images/expand_blue.jpg" SuppressPostBack="true" /> The first panel (Panel2) is essentially the header where the image to expand/collapse the panel is located The majority of the actual content is in the...
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