cytology immunocytochemistry and in situ hybridization
Báo cáo khoa học: "Comparison of immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) assessment for Her-2 status in breast cancer" ppsx
Ngày tải lên :
09/08/2014, 04:21
... results, including variability in tissue fixation and processing, variable sensitivity and specificity of commercially available antibodies, and differences in scoring criteria with considerable interobserver ... variability in interpretation of results [23] Our study is trying to answer a simpler question about the concordance between IHC and FISH in our local setting in Guilin city of China, using some ... methods used in clinical diagnosis, and certain kits or antibodies are approved by the FDA (U.S Food and Drug Administration) IHC is easy to perform and relatively cheap, and is predominantly used...
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Tìm hiểu kỹ thuật lai huỳnh quang tại chỗ FISH (Florescence In Situ Hybridization) trên vi sinh vật
Ngày tải lên :
30/10/2012, 11:02
... DNA in bovine erythrocytes by slot-blot and in situ hybridization with a PCRmediated digoxigenin-labeled DNA probe J.Vet Diagn Invest 7, 465–472 [47] Gebhart, C.J., 1994 Specific in situ hybridization ... detection of the genus Serpulina, S hyodysenteriae and S pilosicoli in porcine intestines by fluorescent rRNA in situ hybridization Mol Cell Probes 12, 323–330 [20] Brown-Howland, E.B., 1992 Development ... colonization of large intestines of streptomycin-treated mice Infect Immun 67, 6152–6156 [37] Felske, A., 1998 In situ detection of an uncultured predominant Bacillus in dutch grassland soils Appl Environ...
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ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS
Ngày tải lên :
05/09/2013, 08:40
... fluorescence in situ hybridization (FISH) and microsensor techniques have a high spatial (at a single-cell resolution) and temporal resolution and great potential, and provide reliable and direct information ... nitrifiers and heterotrophs in mixed population biofilms Biotechnol Bioeng 50:24-35 Okabe, S., H Satoh, and Y Watanabe 1999a In situ analysis of nitrifying biofilms as determined by in situ hybridization ... or t-RFLP Cloning PCR Clone Libraries Sequencing of individual bands Sequencing of clones Southern Hybridization analysis Genetic Fingerprints Adaptation of culture conditions Hybridization analysis...
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in situ hybridization protocols, 2nd
Ngày tải lên :
11/04/2014, 09:47
... and differentiation by the subsequent staining with Giemsa stain is improved (see Note 1) 3.6.1 Staining for GBG- and RBG-Banding Sensitize slides in a Coplin jar for 30 at room temperature in ... Washing and Detection Do not allow slides to dry out at any stage All washing is performed in a Coplin jar Remove coverslips and wash 3× for in prewarmed solution A in a Coplin jar in a shaking ... metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries Hum Genet 80, 224–234 Collins, C., Lin Kuo, W., Segraves, R., Fuscoe, J., Pinkel, D., and Gray,...
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prins and in situ pcr protocols
Ngày tải lên :
11/04/2014, 10:11
... Hopman Introduction Primed in situ (PRINS) labeling has become an alternative to in situ hybridization (ISH) for the localization of nucleic acid sequences in cell preparations (1-4) In the PRINS ... chromosome and centromeres with biotin/PO-DAB (brown) and digoxigenin/APase-fast red (red), respectively, in a human lymphocyte metaphase spread after double-target PRINS, hematoxylin counterstaining and ... chamber, and incubate for h at 37°C in an incubator 11 Dehydrate the slides as described in step and au-dry before running the next PRINS reaction with another primer and different reporter 12 Finally,...
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Báo cáo hóa học: "Prognostic Impact of MiR-155 in Non-Small Cell Lung Cancer Evaluated by in Situ Hybridization" pot
Ngày tải lên :
18/06/2014, 16:20
... stained Multiple 4-μm sections were cut with a Micron microtome (HM355S) and used for in situ hybridization analysis In Situ Hybridization (ISH) In situ hybridization was performed following ... KE, TB and KL carried out and supervised the ISH SWS and KE scored the cores KAS, SAS, SA and HS contributed in the clinical and demographic database and KAS and SAS in making the TMAs RB and LTB ... TMAs RB and LTB supervised and participated in the study design, result interpretation and in the writing All authors read and approved the final manuscript Competing interests The authors declare...
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Báo cáo hóa học: " A Novel Method to Fabricate Silicon Nanowire p–n Junctions by a Combination of Ion Implantation and in-situ Doping" docx
Ngày tải lên :
22/06/2014, 00:20
... Conclusion In conclusion, we have demonstrated a novel method to form p–n junction NW diodes by combining two well- 123 established doping techniques in- situ doping and ion implantation, in succession ... are in direct contact with the p–n junction Therefore we think that surface recombination is indeed playing a major role in the current conduction mechanism across the p–n junction resulting in ... should result in B-doped—intrinsic (p–i) type NWs, since the boron diffusion in silicon is negligible at 525°C [7], i.e., the B atoms incorporated in the lower half will not diffuse into the upper...
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Luận văn : ỨNG DỤNG PHƯƠNG PHÁP IN SITU HYBRIDIZATION ĐỂ CHẨN ĐOÁN MẦM BỆNH WSSV (White Spot Syndrome Virus) TRÊN TÔM SÚ (Penaeus monodon) VÀ TSV (Taura Syndrome Virus) TRÊN TÔM THẺ CHÂN TRẮNG (Penaeus vannamei) part 3 doc
Ngày tải lên :
28/07/2014, 02:21
... Odile Michel and Xue-Ming Tang, 1997 Biotin and Digoxigenin as Labels for Light and Electron Microscopy in situ Hybridization Probes: Where we stand? The Journal of Histochemistry and Cytochemistry ... Procedures Online 3: – Japan 18 Baumgart E., Schad A., Grabenbauer M., 2001 In situ hybridization: general principles and application digoxigenin – labeled cRNA for detection of mRNAs In Immunocytochemistry ... virus (GAV) by in situ hybridization during acute and chronic infections of Penaeus monodon and Penaeus esculentus Diseases of Aquatic Organisms 56: – 10 Queenlands Bioscience Precint, 306 Carmody...
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Luận văn : ỨNG DỤNG PHƯƠNG PHÁP IN SITU HYBRIDIZATION ĐỂ CHẨN ĐOÁN MẦM BỆNH WSSV (White Spot Syndrome Virus) TRÊN TÔM SÚ (Penaeus monodon) VÀ TSV (Taura Syndrome Virus) TRÊN TÔM THẺ CHÂN TRẮNG (Penaeus vannamei) part 2 ppsx
Ngày tải lên :
28/07/2014, 02:21
... mẫu paraffin Đặt mẫu xử lý vào khuôn inox, dùng bình rót paraffin (5 paraffin : sáp ong) vào khuôn chứa mẫu, cố định mẫu cho nằm yên dƣới đáy tách rời Sau đặt khuôn inox chứa mẫu paraffin lên bề ... 21 phân tử lai đƣợc phát cho ligand avidin (hay streptavidin) vào phản ứng Avidin (hay streptavidin) trƣớc có gắn với nhóm phát huỳnh quang hay enzyme xúc tác cho ... dịch Blocking buffer 1X, chất blocking dung dịch khóa phân tử lai không đặc hiệu lại Ủ mẫu với dung dịch Anti – Digoxigenin (Anti – DIG) có gắn Alkaline phosphatase (AP), Anti – Digoxigenin kết...
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Luận văn : ỨNG DỤNG PHƯƠNG PHÁP IN SITU HYBRIDIZATION ĐỂ CHẨN ĐOÁN MẦM BỆNH WSSV (White Spot Syndrome Virus) TRÊN TÔM SÚ (Penaeus monodon) VÀ TSV (Taura Syndrome Virus) TRÊN TÔM THẺ CHÂN TRẮNG (Penaeus vannamei) part 1 pdf
Ngày tải lên :
28/07/2014, 02:21
... phƣơng pháp phòng bệnh virus đốm trắng II.5 PHƢƠNG PHÁP IN SITU HYBRIDIZATION II.5.1 Sơ lƣợc phƣơng pháp In situ hybridization Phƣơng pháp In Situ hybridization (ISH) đƣợc phát vào năm 1969 công Gall ... : In Situ hybridization PCR : Polymerase Chain Reaction ELISA : Enzyme Linked Immuno Sorbent Assay RT – PCR : Reverse – transcription polymerase chain reaction PBS : Phosphate Buffered Saline ... 14 II.4.2.5 Phòng trị bệnh 14 II.5 PHƢƠNG PHÁP IN SITU HYBRIDIZATION 14 II.5.1 Sơ lƣợc phƣơng pháp In situ hybridization 14 II.5.2 Khái niệm lai phân tử ...
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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf
Ngày tải lên :
07/08/2014, 17:22
... enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled probe, ... Okjin Kim Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by in situ hybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated ... of intracellular viral DNAs in paraffin sections J Vet Med Sci 2003, 64, 231-235 73 Kim O, Chae C In situ hybridization for the detection and localization of porcine epidemic diarrhea virus in...
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Báo cáo y học: "yclooxygenases and prostaglandin E2 receptors in growth plate chondrocytes in vitro and in situ – prostaglandin E2 dependent proliferation of growth plate chondrocytes" ppsx
Ngày tải lên :
09/08/2014, 08:22
... However, inadequate information is available on in situ expression of both COX-1 and COX-2 within the growth plate to correlate in vitro findings with the in situ situation PGE2, the principal ... analysis and interpretation of data and was involved in drafting the manuscript CJK was involved in data interpretation, drafting the manuscript and revised it critically for the physiological and ... EP1, EP2, EP3, and EP4 in the growth plate and compared this profile with their expression in cultured growth plate chondrocytes in order to give innovative insights into in situ -in vitro correlations...
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báo cáo khoa học: "mRNA detection of individual cells with the single cell nanoprobe method compared with in situ hybridization" doc
Ngày tải lên :
11/08/2014, 00:22
... results also showed that dark staining was observed around the nucleus predominately, indicating that β-actin mRNAs existed mainly in the vicinity of the nucleus In order to analyze the correlation ... insertion with the AFM probes β-actin mRNAs were detected mainly in the vicinity of the nucleus Usually β-actin mRNA is known to exist mainly in the vicinity of the nucleus, and these results agreed with ... localization in single living cells [12-14] In those studies, we showed that the SCN method has the possibility of compensating for the disadvantages of ISH in the case of the single-cell study Results and...
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Báo cáo y học: " Caveolin-1 and -2 in airway epithelium: expression and in situ association as detected by FRET-CLSM" pps
Ngày tải lên :
12/08/2014, 16:20
... determine whether cav-1 and cav-2 are in close apposition in airway epithelial cells in situ, thereby indicating an association of both proteins and formation of hetero-oligomers Distinct increase ... 20 and washed) and incubated for1 h in 5% normal goat serum containing 5% BSA in 0.005 M phosphate-buffered saline (PBS) Primary antibodies were diluted in 0.005 M phosphate buffer containing ... agonist binding [21,22] These receptors are involved in the regulation of ciliary function [23,24] In addition, several proteins involved in Ca2+-dependent signaling processes, including the Ca2+-pump...
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Báo cáo sinh học: "Localization of leucocyte interferon gene in the q2.5 region of pig chromosomel by in situ hybridization" potx
Ngày tải lên :
14/08/2014, 19:23
... by in situ hybridization Cytogenet Cell Genet 39, 220-223 Lefevre F & Labonnardi6re C (1986) Molecular cloning and sequencing of a gene incoding biologically active porcine interferon alpha J Interferon ... explain the efficiency of in situ hybridization This observation confirms the one given by Lin et al 1985 We have also modified the washings after hybridization They are less stringent and, in ... concerning the washings after hybridization: the slides were first rinsed in x SSC at room temperature, and in 50% formamide and x SSC at 37 °C for lh, then carefully washed in x SSC Labeling was...
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First principles calculations and in situ characterizations
Ngày tải lên :
14/09/2015, 14:05
... is cut into slices with mm in diameter, then the slice is thinned into 10 µm around by using grinding and dimpling apparatus, and finally the thin sample is post-thinned using ion milling The ... (GeO2 and Ge3 N4 ), and to study, both experimentally (using XPS and TEM) and theoretically (using first-principles calculations), the electronic, intrinsic defect, and interface properties and ... at the interface may tune band offsets Theoretically, the procedure of obtaining band offset by using first-principle calculations is actually similar to that of using XPS to determine band offsets...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 1
Ngày tải lên :
14/09/2015, 14:10
... consisting of mechanical consolidation, sintering and extrusion Mechanical testing however often exhibits controversial results and the understanding on deformation behavior of fine grained/nc ... milling media (balls and vial), (ii) process control agent (PCA) and (iii) atmosphere during processing Residual porosity may result from poor interparticle bonding during consolidation and sintering ... due to grain-boundary sliding and/ or diffusional mass transfer via grain-boundary diffusion As such, it is essential to understand and investigate the mechanical behaviors in fine grained materials...
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Processing and mechanical properties of pure mg and in situ aln reinforced mg 5al composite 2
Ngày tải lên :
14/09/2015, 14:10
... relatively large final grain size was obtained Enhanced recovery during milling was confirmed by the corresponding low internal strain The internal strain during milling showed inverse grain size dependence ... the reinforcementmatrix interfaces are clean, resulting in strong interfacial bonding; 35 Literature Review in- situ formed reinforcing particles are finer in size and their distribution in the ... vacuum or in a reducing medium such as toluene and performing powder handling in an argon-filled glove box can minimize the atmospheric contamination [117] The reduction in grain size in powder...
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