creactive protein structure affects function

Ngày tải lên: 23/03/2014, 15:20

... (KIND) is a putative protein protein interaction module. Four KIND-containing proteins, Spir-2 (actin-nuclear factor), PTPN13 (protein tyrosine phosphatase), FRMPD2 (scaffold protein) and very-KIND ... compilation ª 2011 FEBS 1655 Introduction Protein protein interactions play important roles in the molecular recognition and functional modulation between proteins in many signal transduction path- ways ... for 1 h, followed by rotation with protein A-sepharose or protein G-sepharose (GE Healthcare UK Ltd) at 4 °C. Proteins immunoprecipi- tated with the antibody protein A- or G-sepharose com- plexes...

Ngày tải lên: 14/02/2014, 19:20

... these proteins, all tetrameric, showed signifi- cant similarity to the published structures of transthyretin. By way of example, a comparison of the structure of S. dublin TLP with the structures ... 3D structures of transthyretin from various organ- isms have been well characterized. The first transthyre- tin crystal structure to be solved (that of human) was published in 1978 [34]. The Protein ... site of TLP appears to have been sufficient for the acquisition of new functional properties of the protein whilst its over- all structure was unchanged. Furthermore, the distri- bution of TLPs in...

Ngày tải lên: 07/03/2014, 00:20

... in a constitutively active state by CXXC domain proteins that bind to non-methylated CG sequences. These proteins include MLL1 and the CG-binding protein Cfp1 that itself recruits both Set1 and ... 3822– 3831. 80 Melcher M, Schmid M, Aagaard L, Selenko P, Laible G & Jenuwein T (2000) Structure- function analysis of SUV39H1 reveals a dominant role in heterochromatin organization, chromosome ... Perturbation of nucleosome structure by the erythroid transcription factor GATA-1. J Mol Biol 279, 529–544. 244 Armstrong JA & Emerson BM (1996) NF-E2 disrupts chromatin structure at human beta-globin...

Ngày tải lên: 14/02/2014, 18:20

... circles 0.6:1 (redrawn from Koehl, 1995). BODY SIZE AND SUSPENSION FEEDING 21 Body Size The Structure and Function of Aquatic Ecosystems Edited by ALAN G. HILDREW School of Biological and Chemical ... Allen, A. P. & Brown, J. H. (2006). Food web structure and dynamics: reconciling alternative ecological currencies. In Ecological Networks: Linking Structure to Dynamics in Food Webs, eds. M. ... in the rate of fluid passing over or through feeding structures. To counter this, many animals extend their bodies, or just their feeding structures, up through the velocity gradient where they...

Ngày tải lên: 17/02/2014, 19:20

... stabilizers of structured forms of proteins and are often employed in studies aimed at clarifying aspects of the folding process of proteins [34], including cytochrome c [18]. Concerning this last protein, ... mechanism(s) and the factors affecting protein stabilization, in this article we extend our knowledge on anion protein interactions by determining the effect on the protein produced by anions carrying ... dena- tured (ÆÆÆÆ) protein are shown for comparison. Protein concentration: 10 l M. Other experimental conditions were as described in the legend to Fig. 1. F. Sinibaldi et al. Anion-modulated structure...

Ngày tải lên: 19/02/2014, 05:20

... program PROTEIN EXPLORER http://www.proteinexplorer.org. 27 Ó FEBS 2004 Plant aspartic proteinases (Eur. J. Biochem. 271) 2069 REVIEW ARTICLE Structure and function of plant aspartic proteinases Isaura ... barley-grain aspartic proteinase: a structural rationale for observed hydrolytic specificity. FEBS Lett. 352, 131–136. 37. O’Brien, J.S. & Kishimoto, Y. (1991) Saposin proteins: structure, function, and ... Tecnologia). References 1. Davies, D.R. (1990) The structure and function of the aspartic proteinases. Annu. Rev. Biophys. Biophys. Chem. 19, 189–215. 2. Dunn, B.M. (2002) Structure and mechanism of the pepsin-like family...

Ngày tải lên: 19/02/2014, 12:20

... understanding of their structure- activity relationships, may start to provide a rational way to develop additional pharmacological tools for the elucidation of nAChR structure and function. Ó FEBS ... pentameric quaternary structure of this protein (Fig. 1) and have provided valuable information about the channel architecture and dimensions. A deeper insight into the molecular structure, in particular the ... (b2or b4) subunit to form functional channels [2,3,9]. However, pairwise combinations of the a6withtheb2orb4 subunit resulted in protein aggregation or very inefficient expression of functional channels...

Ngày tải lên: 19/02/2014, 12:20

... insects The structure and function of b-helical antifreeze proteins Steffen P. Graether and Brian D. Sykes CIHR Group in Protein Structure and Function, Department of Biochemistry and Protein Engineering ... antifreeze protein; CfAFP, Choristoneura fumi- ferana antifreeze protein; DAFP, Dendroides canadensis antifreeze protein; DcAFP, Daucus carota antifreeze protein; INP, ice-nucleation protein; LpxA, ... describe the structure and dynamics of each protein, and present a comparison of s bwAFP and TmAFPwitheachotherandwithproteinsthathavea similar fold. Structure of sbwAFP and TmAFP The structure...

Ngày tải lên: 19/02/2014, 16:20

... solution structure of the C-domain. (A) Stereo view of the ensemble of the final 48 calculated structures. Twenty-four structures of the closed protein conformer are shown in red, and 24 structures ... Mantsyzov et al. NMR structure and function of the eRF1 C-domain FEBS Journal 277 (2010) 2611–2627 ª 2010 The Authors Journal compilation ª 2010 FEBS 2623 NMR solution structure and function of the ... in solution. The overall fold and the structure of the b-strand core of the protein in solution are similar to those found in the crystal structure. The structure of the minidomain (residues...

Ngày tải lên: 06/03/2014, 11:20

... produces two protein protein interfaces. (B) One protein protein interface generated by two-fold NCS. (C) Other protein protein inter- faces also generated by two-fold NCS. R. Valverde et al. Structure ... the protein protein interaction. A B Fig. 12. Schematic representation of protein protein surfaces of free and RNA-bound Nova-2 crystals. The schematic in (A) and (B) is based on the protein protein ... which parts of the large proteins were involved in associa- AB Fig. 13. Schematic representation of protein protein interfaces in the structure of PCBP. (A) was generated using Protein Data Bank coordi- nates...

Ngày tải lên: 07/03/2014, 05:20

... and secondary structures (I, II, and III). a-helices are indicated in bold, b-sheets are underlined. Mutated cysteine residues of GlcNAc kinase are labeled with a star. Ó FEBS 2002 Structure and function ... S., Guinea, B., Sanchez, L., Roca, R., Valencia, A. & Bernad, A. (2002) Functional Ó FEBS 2002 Structure and function of GlcNAc kinase (Eur. J. Biochem. 269) 4217 ... GlcNAc kinase cDNAs were expressed in E. coli BL21 cells. The proteins were fused to a His-tag, which allowed purification of the proteins by Ni-nitrilotriacetic acid chromatography and detection...

Ngày tải lên: 08/03/2014, 10:20

... supercoiling. relaxed supercoiled Proteins often bind to specific sequences of DNA. Example: Restriction enzyme EcoRI binds to the DNA sequence 5’-GAATTC-3’ 3’-CTTAAG-5’ Protein – DNA interaction Genomes Source ... introns. Ribonucleotides have a 2’-OH Deoxyribonucleotides have a 2’-H Chemical Structure of DNA vs RNA DNA molecules are packaged in the cell as structures called chromosomes. Bacteria have a single chromosome. ... (genome) RNA is synthesized in the nucleus and exported to the cytoplasm Nucleus Cytoplasm DNA RNA (mRNA) Proteins replication transcription translation In eukaryotes, genes contain exons (coding regions) and...

Ngày tải lên: 13/03/2014, 16:42

... Nucleus Nucleus  Structure Structure  Nuclear envelope Nuclear envelope • Two Phospholipid bilayers with protein Two Phospholipid bilayers with protein lined pores lined pores  Each pore is a ring of 8 proteins ... Reticulum Endoplasmic Reticulum  Function RER Function RER • Proteins are modified as they move through Proteins are modified as they move through the RER the RER • Once modified, the proteins are packaged ... fiber of DNA with proteins attached proteins attached  Chromatin – all of the cell’s DNA and the Chromatin – all of the cell’s DNA and the associated proteins associated proteins Why Are...

Ngày tải lên: 13/03/2014, 19:31

... detoxification 3. 3. Transport of proteins from RER Transport of proteins from RER RER RER SER SER Functions of Eukaryotic Functions of Eukaryotic Cell Features Cell Features Structure Structure Function( s) Function( s) Cilia ... distributes proteins and lipids from ER distributes proteins and lipids from ER Functions of Functions of Eukaryotic Cell Eukaryotic Cell Features Features Structure Structure Function Function Mitochondrion Mitochondrion Captures ... CELLS CELLS Structure and Function Structure and Function Cell = smallest unit of life Cell = smallest unit of life Functions of Eukaryotic Cell Features Functions of Eukaryotic Cell Features Structure...

Ngày tải lên: 13/03/2014, 19:31

... MicroRNA-373, a new regulator of protein phosphatase 6, functions as an oncogene in hepatocellular carcinoma Nannan Wu*, Xuyuan Liu*, Xuemei ... of small noncoding RNAs that function as key reg- ulators of gene expression at the post-transcriptional level. Recently, micr- oRNA-373 (miR-373) has been found to function as an oncogene in testicular ... the protein phosphatase 6 catalytic sub- unit (PPP6C ), a negative cell cycle regulator, was identified as a direct target gene of miR-373 by use of a fluorescent reporter assay. The mRNA and protein...

Ngày tải lên: 14/03/2014, 23:20

Ngày tải lên: 16/03/2014, 16:20

Ngày tải lên: 16/03/2014, 18:20

Ngày tải lên: 17/03/2014, 11:20