... (KIND) is a putative protein protein interaction module. Four KIND-containing proteins, Spir-2 (actin-nuclearfactor), PTPN13 (protein tyrosine phosphatase), FRMPD2 (scaffold protein) and very-KIND ... compilation ª 2011 FEBS 1655Introduction Protein protein interactions play important roles inthe molecular recognition and functional modulationbetween proteins in many signal transduction path-ways ... for 1 h, followed byrotation with protein A-sepharose or protein G-sepharose(GE Healthcare UK Ltd) at 4 °C. Proteins immunoprecipi-tated with the antibody protein A- or G-sepharose com-plexes...
... these proteins, all tetrameric, showed signifi-cant similarity to the published structures oftransthyretin. By way of example, a comparison of the structure of S. dublin TLP with the structures ... 3D structures of transthyretin from various organ-isms have been well characterized. The first transthyre-tin crystal structure to be solved (that of human) waspublished in 1978 [34]. The Protein ... site of TLPappears to have been sufficient for the acquisition ofnew functional properties of the protein whilst its over-all structure was unchanged. Furthermore, the distri-bution of TLPs in...
... in a constitutively active state byCXXC domain proteins that bind to non-methylatedCG sequences. These proteins include MLL1 and theCG-binding protein Cfp1 that itself recruits both Set1and ... 3822–3831.80 Melcher M, Schmid M, Aagaard L, Selenko P, LaibleG & Jenuwein T (2000) Structure- function analysis ofSUV39H1 reveals a dominant role in heterochromatinorganization, chromosome ... Perturbation of nucleosome structure by the erythroid transcription factorGATA-1. J Mol Biol 279, 529–544.244 Armstrong JA & Emerson BM (1996) NF-E2 disruptschromatin structure at human beta-globin...
... circles 0.6:1 (redrawn from Koehl, 1995).BODY SIZE AND SUSPENSION FEEDING 21Body SizeThe Structure and Function of Aquatic EcosystemsEdited byALAN G. HILDREWSchool of Biological and Chemical ... Allen, A. P. & Brown, J. H.(2006). Food web structure and dynamics:reconciling alternative ecological currencies.In Ecological Networks: Linking Structure toDynamics in Food Webs, eds. M. ... in the rate of fluid passing over or through feeding structures. Tocounter this, many animals extend their bodies, or just their feeding structures,up through the velocity gradient where they...
... stabilizers of structured formsof proteins and are often employed in studies aimed atclarifying aspects of the folding process of proteins[34], including cytochrome c [18]. Concerning this last protein, ... mechanism(s) and the factors affecting protein stabilization, in this article we extend our knowledgeon anion protein interactions by determining the effecton the protein produced by anions carrying ... dena-tured (ÆÆÆÆ) protein are shown for comparison. Protein concentration:10 lM. Other experimental conditions were as described in thelegend to Fig. 1.F. Sinibaldi et al. Anion-modulated structure...
... program PROTEIN EXPLORERhttp://www.proteinexplorer.org.27Ó FEBS 2004 Plant aspartic proteinases (Eur. J. Biochem. 271) 2069REVIEW ARTICLE Structure and function of plant aspartic proteinasesIsaura ... barley-grain aspartic proteinase:a structural rationale for observed hydrolytic specificity. FEBSLett. 352, 131–136.37. O’Brien, J.S. & Kishimoto, Y. (1991) Saposin proteins: structure, function, and ... Tecnologia).References1. Davies, D.R. (1990) The structure and function of the asparticproteinases. Annu. Rev. Biophys. Biophys. Chem. 19, 189–215.2. Dunn, B.M. (2002) Structure and mechanism of the pepsin-likefamily...
... understanding of their structure- activity relationships,may start to provide a rational way to develop additionalpharmacological tools for the elucidation of nAChR structure and function. Ó FEBS ... pentameric quaternary structure of this protein (Fig. 1) and have provided valuableinformation about the channel architecture and dimensions.A deeper insight into the molecular structure, in particularthe ... (b2orb4) subunit to form functional channels [2,3,9]. However,pairwise combinations of the a6withtheb2orb4 subunitresulted in protein aggregation or very inefficient expressionof functional channels...
... insectsThe structure and function of b-helical antifreeze proteinsSteffen P. Graether and Brian D. SykesCIHR Group in ProteinStructure and Function, Department of Biochemistry and Protein Engineering ... antifreeze protein; CfAFP, Choristoneura fumi-ferana antifreeze protein; DAFP, Dendroides canadensis antifreeze protein; DcAFP, Daucus carota antifreeze protein; INP, ice-nucleation protein; LpxA, ... describe the structure and dynamicsof each protein, and present a comparison of s bwAFP andTmAFPwitheachotherandwithproteinsthathaveasimilar fold. Structure of sbwAFP and TmAFPThe structure...
... solution structure of theC-domain. (A) Stereo view of the ensembleof the final 48 calculated structures.Twenty-four structures of the closed protein conformer are shown in red, and 24structures ... Mantsyzov et al. NMR structure and function of the eRF1 C-domainFEBS Journal 277 (2010) 2611–2627 ª 2010 The Authors Journal compilation ª 2010 FEBS 2623NMR solution structure and function of the ... in solution. The overall fold and the structure of the b-strandcore of the protein in solution are similar to those found in the crystal structure. The structure of the minidomain (residues...
... produces two proteinprotein interfaces. (B) One proteinprotein interface generated by two-fold NCS. (C) Other proteinprotein inter-faces also generated by two-fold NCS.R. Valverde et al. Structure ... the proteinprotein interaction.ABFig. 12. Schematic representation of proteinprotein surfaces offree and RNA-bound Nova-2 crystals. The schematic in (A) and (B)is based on the proteinprotein ... whichparts of the large proteins were involved in associa-ABFig. 13. Schematic representation of proteinprotein interfaces in the structure of PCBP. (A) was generated using Protein Data Bank coordi-nates...
... andsecondary structures (I, II, and III). a-helices are indicated in bold, b-sheets are underlined. Mutated cysteine residues of GlcNAc kinase are labeledwith a star.Ó FEBS 2002 Structure and function ... S., Guinea, B., Sanchez, L., Roca, R., Valencia, A. & Bernad, A. (2002) FunctionalÓ FEBS 2002 Structure and function of GlcNAc kinase (Eur. J. Biochem. 269) 4217 ... GlcNAc kinase cDNAs wereexpressed in E. coli BL21 cells. The proteins were fused toa His-tag, which allowed purification of the proteins byNi-nitrilotriacetic acid chromatography and detection...
... supercoiling.relaxed supercoiledProteins often bind to specific sequences of DNA.Example: Restriction enzyme EcoRI binds to the DNA sequence5’-GAATTC-3’3’-CTTAAG-5’ Protein – DNA interactionGenomesSource ... introns.Ribonucleotides have a 2’-OHDeoxyribonucleotides have a 2’-HChemical Structure of DNA vs RNADNA molecules are packaged in the cell as structures called chromosomes.Bacteria have a single chromosome. ... (genome)RNA is synthesized in the nucleus and exported to the cytoplasmNucleusCytoplasmDNARNA (mRNA)ProteinsreplicationtranscriptiontranslationIn eukaryotes,genes contain exons (coding regions)and...
... NucleusNucleus Structure StructureNuclear envelopeNuclear envelope•Two Phospholipid bilayers with protein Two Phospholipid bilayers with protein lined poreslined poresEach pore is a ring of 8 proteins ... ReticulumEndoplasmic Reticulum Function RER Function RER•Proteins are modified as they move through Proteins are modified as they move through the RERthe RER•Once modified, the proteins are packaged ... fiber of DNA with proteins attachedproteins attachedChromatin – all of the cell’s DNA and the Chromatin – all of the cell’s DNA and the associated proteinsassociated proteins Why Are...
... detoxification3.3.Transport of proteins from RERTransport of proteins from RERRERRERSERSERFunctions of EukaryoticFunctions of Eukaryotic Cell Features Cell Features Structure Structure Function( s) Function( s)Cilia ... distributes proteins and lipids from ERdistributes proteins and lipids from ERFunctions of Functions of Eukaryotic Cell Eukaryotic Cell FeaturesFeatures Structure Structure Function FunctionMitochondrionMitochondrionCaptures ... CELLSCELLS Structure and Function Structure and Function Cell = smallest unit of lifeCell = smallest unit of lifeFunctions of Eukaryotic Cell FeaturesFunctions of Eukaryotic Cell Features Structure...
... MicroRNA-373, a new regulator of protein phosphatase 6,functions as an oncogene in hepatocellular carcinomaNannan Wu*, Xuyuan Liu*, Xuemei ... of small noncoding RNAs that function as key reg-ulators of gene expression at the post-transcriptional level. Recently, micr-oRNA-373 (miR-373) has been found to function as an oncogene intesticular ... the protein phosphatase 6 catalytic sub-unit (PPP6C ), a negative cell cycle regulator, was identified as a directtarget gene of miR-373 by use of a fluorescent reporter assay. The mRNAand protein...