construction expression and purification of rabaptin 5 551 661 fragment
báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx
... not have its own BVMO and the conversion of (-)-camphor was observed with crude cell Figure SDS-PAGE analysis of 2 ,5- DKCMO: lane 1: marker: 150 , 130, 100, 70, 55 , 35, 25, 15 kDa; lane 2: crude ... as the amount of enzyme that catalyzes the oxidation of μmol of substrate per minute Results Cloning, expression and purification of 2, 5- diketocamphane 1,2-monooxygenase The 2 ,5- diketocamphane ... Pseudomonas putida NCIMB 10007: Enantioselective preparation of a precursor of Azadirachtin J Chem Soc Perkin Trans 1: 150 5– 150 5 doi:10.1039/p19 950 00 150 5 Grogan G, Roberts SM, Wan PWH, Willetts AJ (1993)...
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Báo cáo hóa học: " Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance" doc
... http://www.virologyj.com/content /5/ 1/74 4 .5 3 .5 - GP2 bac - NP bac - GP1 bac 3 .5 2 .5 1/100 3.0 1/200 2 .5 O.D O.D 450 nm LASV IgG Assay GP1bac A 2.0 1 .5 1 .5 1.0 0 .5 0 .5 -MsIgG-HRP MsIgG Patient # ... immunoassays, Dr Randal Schoepp kindly provided the following LASV-specific mAbs: NP-specific mAbs 52 273-8 and L2 -54 -6A; GP1-specific mAb L52-74-7A; and GP2-specific mAbs L52-272-7, L52-121-22, and L52-2727, ... for the major bands of each LASV protein The full length 55 -kDa and truncated 46-kDa fragments of LASV Protein Protein Form N-terminal sequence NP NP GP2 GP2 GP1 Full length – 55 kDa Truncated...
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Optimization of expression and purification of HSPA6 protein from Camelus dromedarius in E. Coli
... were induced with varying concentrations of IPTG (0, 10, 25, 50 , 100, 250 , 50 0 and 1000 μM) and further expressions were made for h at 37 °C An equal amount of soluble protein extract was analyzed ... reached basal level and the wash was collected To elute bound protein, gradient was set to 50 %B (50 mM Tris, 50 0 mM imidazole and 50 0 mM sodium chloride, pH 7 .5) at 0 .5 ml/min and the protein was ... concentration on the overexpression of cHSPA6 Lane 1, LMW marker; lane 2, μM; lane 3, 10 μM; lane 4, 25 μM; lane 5, 50 μM; lane 6, 100 μM; lane 7, 250 μM; lane 8, 50 0 μM and lane 9, 1000 μM IPTG...
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Báo cáo khoa học: Expression and characterization of recombinant 2¢,5¢-oligoadenylate synthetase from the marine sponge Geodia cydonium ppt
... p3A2¢p5¢A; 3, p3A2¢p5¢A2¢p5¢A; 4, p3A2¢p5¢A2¢p5¢A2¢p5¢A (m ⁄ z 1493 .5) ; 5, p3A2¢p5¢A3¢p5¢A; 6, p3A3¢p5¢A; 7, p3A3¢p5¢A2¢p5¢A; 8, p3A3¢p5¢A3¢p5¢A; 9, adenosine; 10, mixture of A2¢p5¢A and A2¢p5¢A2¢p5¢A2¢p5¢A; ... A2¢p5¢A2¢p5¢A2¢p5¢A; 11, mixture of A2¢p5¢A2¢p5¢A and A3¢p5¢A2¢p5¢A (m ⁄ z 924.6); 12, A2¢p5¢A3¢p5¢A (m ⁄ z 924.7); 13, putative A2¢p5¢A2¢p5¢A3¢p5¢A (m ⁄ z 1 253 .9); 14, mixture of A3¢p5¢A and A3¢p5¢A3¢p5¢A ... nature of the catalytic domain of 2¢ -5 -oligoadenylate synthetases J Biol Chem 274, 255 35 255 42 22 Rogozin IB, Aravind L & Koonin EV (2003) Differential action of natural selection on the N and...
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iec 60079-15 electrical apparatus for explosive gas atmospheres - construction, test and marking of type of protection n electrical apparatus
... terre 750 < U 100 19 19 12 ,5 12 ,5 100 < U 300 37 ,5 25 19 12 ,5 300 < U 600 63 31 ,5 25 19 V 600 < U 11 000 90 45 37 ,5 25 11 000 < U 13 800 110 55 45 31 ,5 13 800
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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt
... presence and absence of IL-10 *P < 0. 05 and **P < 0.01 versus Cont group (D) Representative western blots for collagen I and III in the presence and absence of 0.1 lM angiotensin II and IL-10 ... from lg of total RNA using M-MLV reverse transcriptase and oligo(dT)18 primer Real-time PCR was performed in a total volume of 25 lL containing 0 .5 lL RT product, 0 .5 lm primers and 12 .5 lL Power ... < 0. 05 versus SD group (C) MSCs were exposed to BAY 11-7082 or MG132, followed by hypoxia ⁄ SD for h and detection of relative mRNA levels of IL-1b and TNF-a by real-time PCR *P < 0. 05 and **P...
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Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc
... reported variants: Noxo1b (AB097667, AF532984, and AF539796), Noxo1c (AF5329 85) , Noxo1a (AY 255 768 and AF532983), and Noxo1d (AY191 359 ) On the basis of the sequence of splice variants, we synthesized ... PI5P PI(3 ,5) P2 PI(4 ,5) P2 PI(3,4)P2 PI(3,4 ,5) P3 B GST-Noxo1γ -PX PI PI3P PI4P PI5P PI(3 ,5) P2 PI(4 ,5) P2 PI(3,4)P2 PI(3,4 ,5) P3 Fig Phosphoinositide-binding activity of the PX domains of Noxo1b and ... compilation ª 2006 FEBS 36 65 Expression and function of Noxo1c R Takeya et al Expression of Noxo1b and Noxo1c in various human tissues To compare the distribution pattern of Noxo1b and Noxo1c, we designed...
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... P212121 51 . 75, 57 .60, 82 .51 28.8–1.92 1. 95 1.92 138441 19106 98 .5 (97.3) 12.9 (5. 5) 8.8 (24 .5) 2079 57 – 143 17 .5 21.6 0.0 05 1 .5 23.4 22.0 20.2 1.3 23.2 16 .5 14.9 1.4 23.1 16 .5 13.7 1.3 23.2 19.9 ... 57 . 05, 81.67 34.4–2.00 2. 05 2.00 83761 1 654 2 97.1 (96.8) 10.2 (3.1) 9.6 (30.7) 2081 57 – 256 16 .5 23.4 0.0 05 (NH4)2SO4 Cocrystallization Chitopentaose Chitopentaose 120 15% glycerol P212121 51 .48, ... P212121 51 . 95, 57 .57 , 82.42 44.0–1.92 1. 95 1.92 187 850 19419 99.8 (97.4) 14.8 (4 .5) 8.7 (32.4) 2083 57 – 141 16.7 20.2 0.009 PEG3 350 Cocrystallization Chitohexaose Chitotetraose 120 – P212121 50 .80,...
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Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus pdf
... full-length cDNA of the cPDI was inserted into an expression vector, pET24a, which contains an N-terminal His6 tag Expression and purification of cPDI and hPDI The expression plasmid of cPDI was transformed ... Folding of omega-conotoxins Influence of precursor sequences and protein disulfide isomerase Biochemistry 35, 155 47– 155 57 Craig AG, Bandyopadhyay P & Olivera BM (1999) Post-translationally modified ... PDI)1)] % of cPDI Oxidase activityb [102 · (lM)1Æmin)1)] % of cPDI Isomerization activityb (lMÆmin)1ÆlM PDI)1) % of cPDI Conus Human 5. 74 ± 0.36 5. 38 ± 0 .52 100 94 9.24 ± 0.83 10.18 ± 0 .57 100 110...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... 2 05 and 2 15 nm, indicating that Rv1399c is composed of 22% of a-helices, 25% of b-strand and 39% of random coil The catalytic triad is located at the bottom of a small ˚ ˚ pocket, 20 A length and ... – – 9 .5 57.6 76.7 68.1 71 .5 80 – – – – 80 .5 486 9 95 1000 1 050 1 350 5. 9 35 48 42.6 44.7 50 Ó FEBS 2004 Biochemical characterization of Rv1399c from M tuberculosis (Eur J Biochem 271) 3 957 Table ... sheet is composed of parallel b strands associated to an antiparallel strand (b2) and is surrounded by helices (a1, a2, a3, a7 and a8) The second domain consists of helices a4, a5 and a6 all clustered...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... concentration of 20 50 lgÆmL)1 during 48 h at °C Refolded hVb5.2mCb 15 was concentrated and dialyzed extensively against NaCl/Pi and affinity purified using the anti-mouse Cb mAb (H57 -59 7) [24, 25] Alternatively, ... SSA(C26S)–Vb5.2 SSA(C26S)–Vb2.1 SSA(C26S)–Vb1 34.4 – 5. 1 29.9 – 5. 6 34 .5 ± – 5. 5 ± 32.0 ± – 5. 1 ± 10.0 – 10.8 10.7 – 9.1 0.6 0.2 0 .5 0.1 no Cys in the mature protein sequence (TSST-1, SPEB, SMEZ1 and ... Immobilization of the SAgs instead of TCRs yielded a higher binding constant of the former, which was similar for both complexes, wtSSA–Vb5.2 and SSA(C26S)– Vb5.2 Different concentrations of human Vb2.1 and...
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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc
... 5. 00 10.00 15. 00 10.00 20.00 15. 00 F Abundance 160000 120000 80000 40000 Time > 5. 00 20.00 25. 00 30.00 20.0 25. 00 30.00 40.00 45. 00 40.00 45. 00 G Abundance 450 000 350 000 250 000 150 000 50 000 5. 00 ... 16000 80000 Time > 5. 00 10.00 20.00 15. 00 25. 00 30.00 D Abundance 240000 160000 80000 Time >0 5. 00 10.00 15. 00 20.00 25. 00 30.00 35. 00 40.00 45. 00 25. 00 30.00 35. 00 40.00 45. 00 35. 00 E Abundance ... 200000 Time > 5. 00 10.00 15. 00 20.00 25. 00 30.00 35. 00 40.00 45. 00 35. 00 40.00 45. 00 35. 00 40.00 45. 00 B Abundance 3000000 2200000 1400000 600000 Time > 5. 00 10.00 15. 00 20.00 25. 00 30.00 C Abundance...
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Báo cáo khoa học: Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis pdf
... a 155 bp 5 -UTR and a 254 bp 3¢-UTR including a poly(A) tail of 15 bp (Fig 1A) The cDNA of HRT2 was 1 051 bp in length containing an 852 bp ORF (Fig 1B) and with 92% sequence homolgy to that of ... amplify unknown 3¢- and 5 -end sequences of HRT cDNAs by using 3¢- and 5 -RACE strategies The amplified products of the 3¢-end gave two distinct cDNA fragments which were 689 and 55 7 bp in length, ... temperature control of 30 s at 95 °C, 30 s at 50 °C and at 72 °C with a preheat at 95 °C and a 10 final extension at 72 °C using primers, S1 (5 -GCAAATGCAACTGGA AGCGG-3¢) and A1 (5 -ACAGCCTGCTAGCAAAGA...
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Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx
... overlay [17] of a nitrocellulose blot of a gel containing 40 lg of platelet protein, 0 .5 lg of GST and 0 .5 lg of GST±Rab proteins, except for GST±Rab5A (0.1 lg); (C) an immunoblot of a gel containing ... Mg2+free Ratio of GTPase activities (10 mM Mg2+free /5 lM Mg2+free) GST-Rab31 GST-Rab31Q64L GST-Rab32 GST-Rab32Q85L 0.2 05 0. 057 (8) ± 0.3 35 0.071 (8) ± 0.1 95 0.262 0.498 0 .51 5 1. 15 0.13 (8) ... the presence of lM [35S]GTP[S] and the indicated concentrations of Mg2+free, and is expressed as mol of [35S]GTP[S] bound per 100 mol of protein Mean values SE from the numbers of separate protein...
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Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx
... Effect of temperature on activity of AATB3 (pH 7.2) (B) Thermostability of AATB3 The enzyme was pre-incubated at 40, 50 , 60 or 65 °C for 5, 15 or 30 before the assay (C) Effect of pH on activity of ... specificity and kinetics parameters The purified AATB3 was optimally active at 45 °C (at pH 7.2), and more than 80% of the maximum activity was retained in the temperature range 25 55 °C (Fig 5A) After ... aatB3 fragment was inserted into KpnI and EcoRI sites of pET30a(+) for the expression of protein AATB3; T7 promoter-based expression vector; Kmr The aatB3 fragment was inserted into KpnI and EcoRI...
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Báo cáo khoa học: Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress pot
... decreased to 156 ± 50 and 44 .5 ± 12.8 fmolÆmL)1 hemolymph (n = 9, P < 0. 05) , respectively Effects of stresses on the levels of glucose and lactate in hemolymph and gene expression in the ES and PO Hypoxia ... maenas Endocrinology 146, 55 45 55 51 25 Rome MS, Young-Williams AC, Hines AH, Goodison MR & Aquilar R (20 05) Linking temperature and salinity tolerance to winter mortality of Chesapeake Bay blue ... 3¢-UTR ( 1 .5 kbp) The amplicon (132 bp) produced from the nested PCR of 5 -RACE cDNA contained a 5 -UTR and the remaining sequence of the 5 -end of PO-CHH The complete cDNA sequence of PO-CHH,...
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Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt
... Institute PCR was used to amplify the portion of mouse genomic DNA that was missing in the Sanger database The CB1 cDNA (AY52 255 4) and genomic DNA (AY52 255 5) sequences were submitted to GenBank RNase ... and known amounts of each standard using each set of primers HPRT cycling conditions were 10 at 95 °C, 45 cycles of denaturation ( 95 °C for 15 s), annealing (63 °C for s), and extension (72 °C ... )318, +109, +201 )267 )219, )138, +206 ) 153 )53 )52 )52 +222 +260 +423 +4 65 +466 a Numbers refer to the position of the 5 -end of the conserved matrix of each response element in relation to the...
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Báo cáo khoa học: Bovine tryptases cDNA cloning, tissue specific expression and characterization of the lung isoform ppt
... of 58 °C, using the primer pair Met (nt 1–20, 5 -ATG CTCCATCTGCTGGCGCT-3¢, designed on the basis of the 5 RACE experiments reported below) and Coda [5 -CGCGCGCG(T)16)3¢] [29] and sequenced 5 ... characterization and amino-termoinal sequence Arch Biochem Biophys 258 , 55 5 56 3 Vanderslice, P., Craik, C.S., Nadel, J.A & Caughey, G.H (1989) Molecular cloning of dog mast cell tryptases and a related ... amplification; C5/N10 for intron V amplification Sequences of primers Met, C1, N9 and N10 are reported above Other primers used are: C5, 5 -CCGTCGTGGAGAACAGTGTC-3¢ (nt 53 0 54 9); C6, 5 -TGTCCGCCCCGTTCTTAACGCTGTA-3¢...
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Báo cáo Y học: Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme pdf
... 231, 259 –2 75 16 Candy, D.J & Kilby, B.A (1 958 ) Site and mode of trehalose biosynthesis in the locust Nature 183, 158 4– 159 5 17 Murphy, T.A & Wyatt, G.R (19 65) The enzymes of glycogen and trehalose ... molecular mass of 55 863 Da A 1 .5- kb PCR product was amplified from M tuberculosis H37Rv genomic DNA using the oligonucleotide primers DC 154 (5 -ACTCGAGAGCATATGGCTCC CTCG-3¢) and DC 155 (5 -AGCGGGATCCGCTT ... carried out with 1 .5 mM MgCl2 and at an annealing temperature of 57 °C The resulting PCR product was digested with NdeI and BamI, and ligated with the expression vector pET15b (Novagen), which...
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Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc
... include CR1 (33–317), CR2 ( 356 –4 15) , CR3 (420– 451 ), CR4 (4 65 51 9), CR5 (52 8 54 4), CR6 (55 5 56 7), and CR7 (58 1–609) Residues that are identical among the Conus carboxylase and the vertebrate carboxylases ... proprothrombin), proFIX18 (residues )18 to )1 of proFactor IX), proFIX28 (residues )18 to +10 of proFactor IX), pro-e-TxIX/12 (residues )12 to )1 of e-TxIX precursor), e-TxIX12 (residues 1–12 of e-TxIX), ... Furie, B (1996) Biosynthesis of prothrombin: intracellular localization of the vitamin K-dependent carboxylase and the sites of gamma-carboxylation Blood 88, 258 5– 259 3 15 Kuliopulos, A., Nelson,...
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