computational evaluation of protein stability change upon mutations
Báo cáo lâm nghiệp: "In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker" ppsx
... were performed in 25 µL: 2.0 µL of 1.25 mM each of dNTP’s, 15 ng of the primer, 1× Taq polymerase buffer, 0.5 U of Taq DNA polymerase (Genei, India) and 20 ng of genomic DNA DNA amplification ... test at the level of 5% + Callusing at the basal end Table III Analysis of variance for the effects of BAP, Ads, NAA and IAA on production of multiple shoots (A) and average number of shoots per ... 3–4 nodes) within weeks of subculture (Tab II) (Fig 1b) Analysis of variance for the effects of cytokinins and auxins on production of multiple shoots and average number of shoots/explant was presented...
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báo cáo khoa học: " Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis" pdf
... content of total protein (mg g -1FW) and the % yield of the extraction of soluble proteins (% of extracted soluble proteins respect to the total content) for the roots and the leaves of the plants ... The aim of this work was to apply a proteomic approach to study the changes in protein patterns of root and leaf organs of maize plants in the first phase of exposure to high availability of nitrate, ... reference gel of the software-assisted analysis the values of pI predicted for any given length of the strip Both Mr and pI of the spots of interest were then determined by using software-automated...
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Structural basis of protein stability at poly extreme crystal structure of amya at 1 6 a resolution
... Refinement is the optimization of a function of a set of observations by changing the parameters of a model During the refinement of a protein structure no data cut-off should be applied and generally ... bodies and wavelengths of incident radiation In Bragg’s model of diffraction as reflection from parallel sets of planes, (Fig 1.1) any of these sets of planes can be the source of one diffracted X-ray ... expansion of a periodic function f(x) in terms of an infinite sum of sines and cosines and makes use of the orthogonality relationships of the sine and cosine functions The computation and study of...
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Structural basis of protein stability at poly extreme crystal structure of amya at 1 6 a resolution 3
... to measure an S-value distribution of the sample which indicated that 70% of the protein was sedimenting with an S-value of around 85-90 S, about 25% of the protein sedimenting 64 between 20-80 ... flow rate of 0.5 ml min-1 61 3.3 RESULTS 3.3.1 Thermal stability at different salt concentrations To understand the relationship between the presence of salt and the thermal stability of AmyA, ... aggregate of AmyA However, these aggregates retain 40% of activity when tested (Fig 3.2) Addition of as low as mM of NaCl reversed most of the protein aggregates into the monomeric form, sedimenting...
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Structural basis of protein stability at poly extreme crystal structure of amya at 1 6 a resolution 4
... halophilic stability? Results from various studies on the effect of salt on the stability of various thermophilic proteins suggest that it is very unlikely The effect of salt on the activity and stability ... acting at both the protein surface and the core of AmyA might contribute collectively and significantly to the thermal stability of AmyA 86 Table 4.8 The number of ion pair networks of AmyA and other ... Hyperthermophilic 100 82 4.3.5 Structural determinants of thermal stability of AmyA To determine the conserved features of thermophilic proteins that are present in AmyA, the AmyA structure was...
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Structural basis of protein stability at poly extreme crystal structure of amya at 1 6 a resolution 2
... 2.3.1 Overview of AmyA structure AmyA consists of a single polypeptide chain of 488 residues and comprises a total of 18 α-helices and 20 β-strands The overall fold of AmyA consists of three distinct ... regions of proteins play a major role for protein stability at extreme environments All known halophilic protein structures possess an overall acidic surface This conserved acidic surface of halophilic ... kcal/mol to protein stability (Serrano et al, 1991) 2.1.1.5 Ion-pair Salt bridges are formed by spatially proximal pairs of oppositely charged residues in native protein structures Often salt-bridging...
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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
... 1. FT-IR spectra of PA (a) and pullulan (b) 500 Stability and in vivo evaluation of pullulan acetate 555 (2008) also reported that the smaller size of NPs may be in favor of longer circulation ... Worcestershire, UK) The morphology of NPs was observed using TEM (FEI, TECNAI G2F-20, Eindhoven, Holland) The stability of PANs in water In order to study the stability of PANs, the above dispersions ... informahealthcare.com by University of Technology Sydney on 03/01/11 For personal use only Stability and in vivo evaluation of pullulan acetate 553 aqueous media The hydrophobic core of these selfassembled...
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Tài liệu Báo cáo khóa học: Numerical calculations of the pH of maximal protein stability pptx
... in the field of ÔpH of stability Following search strings were used: Ômaximal stability , Ômaximum stability , Ôoptimal stability , Ôoptimum stability , Ôbest stability , Ôhighest stability and ... balance in protein folding Adv Protein Chem 46, 27–58 33 Elcock, A (1999) Realistic modeling of the denaturated states of proteins allows accurate calculations of the pH dependence of protein stability ... the pH dependence of the folding free energy Some proteins may tolerate a free energy change of 10 kcalÆmol)1 and still be stable, while others became unstable upon a change of only a few kcalÆmol)1...
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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
... parameters of the enzyme-catalyzed reaction change upon modulation of protein stability (DGD°) by osmolytes To investigate the protein stability activity relationship in the presence of osmolytes, ... stabilize proteins [3,4], but they also induce refolding of misfolded proteins [5–8] and remove protein aggregation [9–12] Mechanisms of protein osmolyte interactions, the effect of osmolytes on protein ... effects that urea has on protein function [16–19] Despite significant advances in understanding the effect of osmolytes on protein stability, folding and the activity of proteins and enzymes, the...
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Báo cáo khoa học: Effects of the G376E and G157D mutations on the stability of yeast enolase – a model for human muscle enolase deficiency pdf
... step, dissociation of the dimer into monomers, is accompanied by small changes in the UV spectrum of the protein, changes which were attributed to changes in the environment of several tyrosine ... significantly lower yield of the variants may also be the result of dissociation followed by proteolysis of the monomers The effects of these mutations on temperature stability is not surprising ... dissociation of enolase may be the result of changes in other regions of this same loop In a study of the pressure dissociation [26], we demonstrated that pressure dissociation and inactivation of yeast...
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Báo cáo khoa học: Using directed evolution to improve the solubility of the C-terminal domain of Escherichia coli aminopeptidase P Implications for metal binding and protein stability pptx
... number of mutations per gene The implication of this observation is that the effects of most mutations are small compared with those of G270V, E406G, and R166G Changes at the surface of the protein ... effect of producing a slightly soluble aggregated form of the protein Subsequent changes to the amino acid sequence were far more effective in improving the solubility of the domain In the case of ... ZnCl2 inhibits the activity of AMPP [1] Here, the presence of ZnCl2 in the dialysis buffer led to the precipitation of each of the three proteins Neither the intact protein nor the FEBS Journal...
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Báo cáo Y học: Cytochrome c from a thermophilic bacterium has provided insights into the mechanisms of protein maturation, folding, and stability potx
... responsible for the stability of a thermophilic protein Only a small number of mutant proteins is required to experimentally confirm which amino acids are responsible for overall protein stability This ... underlying protein stability BIOGENESIS OF BACTERIAL CYTOCHROMES c Fig 3D structural comparison of HT c-552 and PA c-551 The main-chain folding of HT c-552 (red) is overlaid with that of PA c-551 ... To characterize the factors that affect protein stability, we attempted to achieve maximal enhancement of the stability of PA c-551 by introducing minimal mutations into spatially separate regions...
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Báo cáo khoa học: and protein bilinear indices – novel bio-macromolecular descriptors for protein research: I. Predicting protein stability effects of a complete set of alanine substitutions in the Arc repressor ppt
... the Arc repressor stability when punctual mutations are induced, and therefore its linear combinations achieve a better description of the effect of mutations upon stability changes Another interesting ... labels) of the vertices of Gm, as a result of the fact that components of the molecular vectors are values of some amino acid property that characterizes each kind of R-chain in the protein The ... cause no signicant changes in protein stability [70] Milla et al [32] subjected each puried mutant of Arc to thermal and urea denaturation experiments The stability of the proteins was checked...
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Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx
... form of dimers [5,14] To understand the importance of capsid protein protein contacts for the stability of coat protein, we determined the stability of dlFG and compared it with the stability of ... determine the role of protein RNA and protein protein interactions in virus stability, measuring the effects of urea, GdnHCl and high pressure on the structure and stability of whole particles ... 300–420 nm Role of the nucleic acid protein complex in the stability of dlFG As shown before, pressure was able to completely denature dlFG above 1.8 kbar To compare the stability of the protein in...
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Báo cáo sinh học: "Pitfalls in the phylogenomic evaluation of human disease-causing mutations" potx
... Tooth agenesis (28/28) Figure MSX1 structure and MAPP evaluation of sequence changes (a) Cartoon of protein [1] showing relative positions of seven conserved motifs (boxes) and missense substitutions ... examined the impact of amino acid changes in human MSX1 using the multivariate analysis of Journal of Biology 2009, 8:26 26.4 Journal of Biology 2009, Volume 8, Article 26 Wilkie protein polymorphism ... limitations of MAPP analysis in this type of situation by regrouping the results of the analysis of Finnerty et al (given in Figure of [1]) according to the phenotype with which the sequence change...
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Báo cáo y học: "Long term evaluation of disease progression through the quantitative magnetic resonance imaging of symptomatic knee osteoarthritis patients: correlation with clinical symptoms and radiographic change" pps
... Comparison of changes in cartilage volume and JSW versus clinical parameters over time The evaluation of the clinical course of all 107 OA patients revealed no significant correlation between the changes ... effects of a bisphosphonate on knee OA structural changes was recently completed A subset of 110 of these patients underwent MRI in addition to the clinical standardized radiograph and biomarker evaluations, ... or NSAIDs, the regimens of which could be changed according to the preference of the rheumatologist and the clinical course of the patient Such regimens, as well as any changes to them, were closely...
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Báo cáo y học: " Development and evaluation of an immunochromatographic strip test based on the recombinant UL51 protein for detecting antibody against duck enteritis virus" ppsx
... and that of the negative number of ICS to the negative number of ELISA is 82.61% b The consistency ratio of the positive number of ICS to the positive number of NT is 19.51% and that of the negative ... purification of the recombinant UL51 protein By the fermenter cultivation, a large number of bacterial cells containing the recombinant UL51 protein were harvest The recombinant protein obtained ... the capture reagent of the ICS Page of Figure SDS-PAGE and western blotting analysis of recombinant UL51 protein (a) SDS-PAGE analysis of recombinant UL51 protein Lane M, molecular mass markers...
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Báo cáo y học: "Hepatitis B virus core protein with hot-spot mutations inhibit MxA gene transcription but has no effect on inhibition of virus replication by interferon a" pptx
... CTL escape mutations of core protein are more frequent in strains of HBeAg negative patients with low levels of HBV DNA J Clin Virol 2009, 46:259-264 Liu Z, Luo K, He H, Hou J: Hot-spot mutations ... analysis of variance (ANOVA) Values of P < 0.05 were considered significant All statistical calculations were performed with SPSS10.5 program Results Influence of HBc protein with hot-spot mutations ... the treatment of CHB The antiviral action of IFN-a is mediated by the induction and activation of at least three IFN-inducible proteins: 2,5-oligoadenylate synthetase (OAS), p68 protein kinase...
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Báo cáo y học: "Characterization of the invariable residue 51 mutations of human immunodeficiency virus type 1 capsid protein on in vitro CA assembly and infectivity" docx
... proteolysis of the Pr55Gag and maturation of CAp24, the NTD of CAp24 refolds into a β-hairpin structure which is then stabilized by formation of a salt-bridge between Pro1 and Asp51 of the processed ... affected Results Viral protein expression of HIV-1 CAp24 mutants We investigated the effects of three HIV-1 CAp24 mutants carrying the D51N, D51E, and D51Q mutations for viral protein expression ... level of viral proteins in released virions, normal- Page of 12 (page number not for citation purposes) Retrovirology 2007, 4:69 http://www.retrovirology.com/content/4/1/69 the level of this protein...
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Báo cáo y học: "implementation and evaluation of the SPRINT protocol for tight glycaemic control in critically ill patients: a clinical practice change" ppsx
... the SPRINT patient pool had a larger proportion of gastrointestinal patients Changes in the economics of health care caused changes in the types of patients admitted to the Christchurch ICU over ... iterations of the cohort selection procedure described in Figure Table shows the change in mortality, both in-ICU and in-hospital, for patients with lengths of stay of at least to days, Page of 15 ... Percentage of measurements between 4.4 and 6.1 mmol/L Percentage of measurements less than Mean insulin usage, U/hour Mean nutrition rate During periods of feeding, kcal/day Entire duration of SPRINT...
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