combined mrna protein expression analysis for pathway mapping and candidate target selection
báo cáo hóa học:" Low RBM3 protein expression correlates with tumour progression and poor prognosis in malignant melanoma: An analysis of 215 cases from the Malmö Diet and Cancer Study" potx
... RFS and OS in both uni- and multivariate analysis Some subjects had no information on one or several markers and missing values were coded as a separate category for categorical variables and ... mm and > mm) were used instead or when clinical stage was excluded from the analysis (data not shown) Information on tumour diameter was only available for 162 (61%) of the patients and therefore ... Median 0.71 Range 0.08-40.00 and disease outcome was examined For survival analysis, data were dichotomized into strong vs negativemoderate intensity for RBM3 Kaplan Meier analysis of the evaluated...
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Báo cáo y học: "Differential expression analysis for sequence count data" ppt
... [http://www-huber.embl.de/users/anders/HTSeq/] 36 DESeq [http://www-huber.embl.de/users/anders/DESeq] doi:10.1186/gb-2010-11-10-r106 Cite this article as: Anders and Huber: Differential expression analysis for sequence ... performed RNA-Seq on replicates of Saccharomyces cerevisiae cultures They tested two library preparation protocols, dT and RH, and obtained three sequencing runs for each protocol, such that for ... middle row to edgeR and bottom row to a Poisson-based c2 test The right column shows the distributions for all genes, the left and middle columns show them separately for genes below and above a mean...
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iOS Forensic Analysis: for iPhone, iPad, and iPod touch doc
... from Wow! eBook iOS Forensic Analysis for iPhone, iPad, and iPod touch ■■■ Sean Morrissey i iOS Forensic Analysis for iPhone, iPad, and iPod touch Copyright © 2010 by Sean ... Chris Cook for his legal analysis and Alex Levinson for his expertise in network forensics Chris Cook is both an attorney and computer forensic analyst He has extensive education and experience ... Collector (CDMC) and Certified Digital Forensic Examiner (CDFE) and was a lead author on the book Mac OS X, iPod, and iPhone Forensic Analysis (Syngress, 2008) Sean also founded Katana Forensics from...
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báo cáo hóa học:" Gene expression profiling for molecular distinction and characterization of laser captured primary lung cancers" ppt
... software, and relative gene expression levels were expressed as the difference in Ct values of the target gene and the control gene ribosomal protein S11(RPS11) RPS11 was selected as reference gene for ... of microtubules and intermediary filaments, as well as keratin and 15 We also looked for genes involved in cell adhesion and migration and found integrin alpha 3, integrin beta and intercellular ... condensation protein G (overexpression in AC vs NT, SCC vs NT and SCLC vs NT was 2.2, 2.1 and 3.2-fold, respectively); collagen, type I, alpha (overexpression in AC vs NT, SCC vs NT and SCLC vs...
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Báo cáo hóa học: " Performance analysis for optimum transmission and comparison with maximal ratio transmission for MIMO systems with cochannel interference" pdf
... simple closedform BER expressions for MRC diversity BPSK in correlated Rayleigh fading and CCI IEEE Trans Commun 57(3), 630–634 (2009) X Zhang, NC Beaulieu, A closed-form BER expression for BPSK ... set to 10 dB for simulation The performances of MIMO systems based on both MRT and OT schemes are investigated and compared, when the signal and interferers are subject to nonfading and Rayleigh ... compensate for each other which makes the performance on BER is symmetric in T and R in the absence of CCI For example, the BER for (T, R) = (3,1) or (3, 2) will be the same as that for (T, R)...
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Báo cáo khoa hoc:" Genetic analysis for mastitis resistance and milk somatic cell score in French Lacaune dairy sheep" ppsx
... Repeatability model for all test-days from DIM 25 to 174 Standards errors between 0.011 and 0.034 Standards errors between 0.029 and 0.119 for genetic correlations and between 0.012 and 0.075 for environmental ... Number of daughters per sire for EBV Reliability for milk yield Reliability for fat or protein contents EBV for milk yield (litres) EBV for fat content (g · L−1 ) EBV for protein content (g · L−1 ... clinical and subclinical mastitis, but adding CM information would increase the efficiency of selection for udder health, and particularly for CM [13,24] Conversely, genetic literature is limited for...
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Báo cáo y học: "Integrative analysis for finding genes and networks involved in diabetes and other complex diseases" ppt
... inferred for two markers at a time due to the high number of missing and non-informative values for many markers Protein interaction networks Genetic interaction analysis was performed for the ... contributions Basic idea and protocol formation: RB and FP Data mining and decision tree analyses: MA, RB and FP Analysis and interpretation of gene-gene interaction data: RB, MA and FP Development ... system for protein- protein interactions: ZMS, KL, EOK, PÍÓ and SB Construction of proteinprotein interaction networks: ZMS, KL, CTW, EOK, PÍÓ and SB Development of statistical method for ranking...
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Efficient progressive collapse analysis for robustness evaluation and enhancement of steel concrete composite buildings
... collapse analysis to obtain the deformation and force demands under sudden column removal event Material damage and geometry nonlinearity need to be considered in progressive collapse analysis for ... advantages and disadvantages of each method and suggests that each analysis should be checked and selected for a target in a progressive way from linear static to nonlinear dynamic analysis In ... equivalent static analysis for robustness evaluation of building In the study, comparison between the results of equivalent static analysis, codified static analysis and nonlinear time-history analysis...
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Expression analysis of y TMT AND FAD2 gene in escherichia coli and transgenic trichoderma reesei
... B and plates were incubated as previously The obtained transformants were further subcultured for generations, and then examined the expression of PfTMT gene 2.2 Molecular analysis of transformants ... slational modifications and biological activity of the protein pPK5-PfTMT vector was used for the expression of 6×His-tagged recombinant proteins in T reseive and the recombinant proteins was easily ... heterologous proteins [179, 169] Based on the efficient expression and the considerable information on regulation of cellulase genes, their promotors are routime used for heterologous protein production...
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báo cáo khoa học: " Association mapping and marker-assisted selection of the lettuce dieback resistance gene Tvr1" potx
... calculated for markers LK1475, Cntg4252, and Cntg11275 when combined intron and exon data were considered and for markers Cntg10192, CLSM9959, and CLSZ1525 that contain exons only Association mapping ... qualitative trait (resistant or susceptible) and used for linkage analysis Association mapping and assessment of population structure Association mapping was performed on a set of 68 accessions from ... developed primers, performed marker and sequence analysis, and assisted in drafting the manuscript LKM, MJT, OEO and RWM developed mapping populations, provided EST data, and revised the manuscript...
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Exergy analysis for combined regenerative Brayton and inverse Brayton cycles
... Energy and Environment (IJEE), Volume 3, Issue 5, 2012, pp.715-730 717 Figure T-s diagram for the combined cycle Exergy analysis and optimization The following assumptions are made for simplicity and ... the system efficiency for open cycle systems In this paper, the exergy analysis for combined regenerative Brayton and inverse Brayton cycles proposed in Ref [10] is performed The purposes of ... been performed in this paper The effects of the effectiveness of the regenerator and other parameters on the exergy performances of the combined cycle are analyzed, and the exergy performances...
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mRNA Expression Analysis
... RefSeq, and more specialized sources Illumina Whole-Genome Expression BeadChips are ideal for applications such as differential expression analysis, disease classiication, pathway analysis, and eQTL ... comprehensive and stable reference for genomic DNA, transcript, and protein products The HumanRef-8, MouseRef-8, and RatRef-12 BeadChips are developed exclusively from the RefSeq database content and target ... calling, and standard iltering by the Analysis Pipeline generate a list of sequence tags and counts This list of tags can be annotated with genomic information and used to analyze differential gene expression...
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Báo cáo khoa học: Identification of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis doc
... levels of expression of XlMGP transcripts IA and IB ODC was used as a loading control RNA extracts used for RT-PCRs were made from pools of five randomly picked embryos Results obtained for egg and ... second functional promoter for the XlMGP gene Luc reporter constructs and subsequent deletion mutant analysis confirmed this hypothesis and provided clear evidence for the presence of two functional ... model, i.e MGP mRNA was consistently found in cells from the chondrocytic lineage and thus associated with cartilage formation and mineralization In conclusion, our data identifies for the first...
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Báo cáo khoa học: Structure, linkage mapping and expression of the heart-type fatty acid-binding protein gene (fabp3 ) from zebrafish (Danio rerio) pot
... quantities of mRNAs, rRNAs and tRNAs are synthesized, some mRNAs are translated immediately into protein while others are stored in an inactive form, ribosomes and mitochondria accumulate, and quantities ... employed for detection of RACK1 mRNA were the same as RT-PCR of fabp3 mRNA (see above) As a negative control, reactions contained all RT-PCR components and specific primers for either fabp3 or RACK1 mRNA, ... in 4% paraformaldehyde for min, in NaCl/Pi twice for three min, and then washed in 1· NaCl/Cit for 20 The fixed tissue sections were immersed in a hybridization buffer containing 50% formamide,...
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Báo cáo hóa học: " Research Article The Wavelet-Based Cluster Analysis for Temporal Gene Expression Data" pptx
... continuous wavelet analysis to transform all expression data by wavelet transform; it decomposes temporal gene expression data in both time and frequency domains In wavelet transform we take a real/complex ... wavelet analysis and the Fourier transformation (FT) are two widely used methods in signal processing In its original form, the FT assumes that the expression signal exists for all time This for ... transformation of gene expression profile Wavelet transformation is an analysis method that uses both time and the frequency domains It allows a time series to be viewed in multiple resolutions, and...
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Báo cáo y học: "A scaling normalization method for differential expression analysis of RNA-seq data" pps
... t-statistic analysis The solid and dashed lines show the library size normalized and TMM normalized Poisson model analysis, respectively The blue and black lines represent the LR test and exact ... total reads for gene g in experimental condition zk Their analysis utilizes an offset to account for the library size and a likelihood ratio (LR) statistic to test for differences in expression ... accounting for within-class biological variability in serial analysis of gene expression (SAGE) BMC Bioinformatics 2004, 5:119 11 Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B: Mapping and...
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Báo cáo y học: "ExpressionPlot: a web-based framework for analysis of RNA-Seq and microarray gene expression data" doc
... Friedman1,2,3* and Tom Maniatis4 Abstract RNA-Seq and microarray platforms have emerged as important tools for detecting changes in gene expression and RNA processing in biological samples We present ExpressionPlot, ... discussion group, and a prototype are available at http://expressionplot.com/ Rationale RNA-Seq has emerged in recent years as the eminent platform for analysis of gene expression and RNA processing ... or GB) and hard drive space to hold the sequencing data and processed files (roughly to GB per lane) In short, ExpressionPlot is a unified solution for gene expression analysis of RNA-Seq and microarray...
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báo cáo khoa học: "Combinatorial peptidomics: a generic approach for protein expression profiling" potx
... of known proteins by using calculated and predicted combinations of digestion and separation features Combinatorial peptidomics is suitable for studying both novel protein targets and for routine ... Heller J and Horwitz J: Involvement of tyrosine and lysine residues of retinol-binding protein in the interaction between retinol and retinol-binding protein and between retinolbinding protein and ... of peptides and proteins Proc Natl Acad Sci U S A 1997, 94:12297-12302 Liu R, Barrick JE, Szostak JW and Roberts RW: Optimized synthesis of RNA -protein fusions for in vitro protein selection Methods...
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báo cáo khoa học: " Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality" pot
... improvement and for the help in bioinformatic analysis of field phenotypic data and correlation analysis This research was financially supported by a grant from the German Ministry of Education and Research ... groups and included transcription regulators, genes induced by abiotic stress, genes responsible for the synthesis of storage proteins and genes related to amino acid and carbohydrate metabolism, and ... included landraces, elite breeding lines, the mapping population parents and H spontaneum This delivered 180 polymorphic sites (SNPs and indels) across both intronic and exonic sequence, and led...
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báo cáo khoa học: " An extensive (co-)expression analysis tool for the cytochrome P450 superfamily in Arabidopsis thaliana" docx
... plant gene Co -expression analysis and pathway mapping Affymetrix expression data for the selected 4,129 probe sets were retrieved and processed as described above for the P450s and the expression ... added and saved in html format Results for each P450 can be found at the 'Pathway Map' webpage for each P450 Expression data and pathway information data for co-expressed genes (r > 0.5 for a ... co-expressed pathways were identified for each P450 and expression dataset The lists of coexpressed pathways can be found from the 'CYPedia' home page following the 'pathway maps' link for each P450...
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