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coli electroporation and selection of galk positive and galk negative bacteria

Báo cáo khoa học: Selection of full-length IgGs by tandem display on filamentous phage particles and Escherichia coli fluorescence-activated cell sorting screening doc

Báo cáo khoa học: Selection of full-length IgGs by tandem display on filamentous phage particles and Escherichia coli fluorescence-activated cell sorting screening doc

Báo cáo khoa học

... development of an E colibased technology, termed E-clonal, for the successful production of soluble full-length IgGs in bacteria and for screening of combinatorial IgG libraries using bacterial ... highest-affinity and lowest-affinity IgGs were determined to have KD values of and 440 nm, respectively Enrichment of high-affinity and moderate-affinity IgGs To evaluate the utility of tandem phage ... PA-specific binders Sequence analysis of the positive clones confirmed the selection of the highaffinity M18 and the moderate-affinity YMF10 To assess the enrichment of M18 and YMF10, phagemid was isolated...
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Báo cáo y học:

Báo cáo y học: "Evolution of the core and pan-genome of Streptococcus: positive selection, recombination, and genome composition" pdf

Báo cáo khoa học

... gain, loss and duplication, and positive selection Gene gain, loss and duplication, and positive selection Core-genome phylogenies of Streptococcus (left), S agalactiae (middle), and S pyogenes ... were MGAS10270 and that leading to SSI-1/MGAS315, and for S Frequency 150 100 50 34 Number of lineages Figure Frequency of positive selection Frequency of positive selection Numbers of genes showing ... Lefébure and Stanhope R71.11 comment Figure selection occurrence per genes and lineages Positive Positive selection occurrence per genes and lineages A black dot indicates positive selection...
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PHOTOREACTIVATION OF ENTEROHEMORRHAGIC E. COLI, VRE AND P. AERUGINOSA FOLLOWING UV DISINFECTION

PHOTOREACTIVATION OF ENTEROHEMORRHAGIC E. COLI, VRE AND P. AERUGINOSA FOLLOWING UV DISINFECTION

Môi trường

... MATERIALS AND METHODS Bacterial Strains One strain of enterohemorrhagic Escherichia coli O26 was provided by Prof M Fukuyama, College of Environmental Health, Azabu University One strain of vancomycin ... Photoreactivation of VRE - 22 - Comparison of UV Dose Required for 90% Inactivation of Tested Bacteria The UV doses required for 90% inactivation of tested bacteria are shown in Figure (Tosa and Hirata, ... Ultraviolet inactivation of selected bacteria and viruses with photoreactivation of the bacteria Wat Res., 21, 687–692 Hassen A., Mahrouk M Ouzari H., Cherif M., Boudabous A and Damelincourt J J...
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the positive and the negative sides of globalization

the positive and the negative sides of globalization

Kỹ năng đọc tiếng Anh

... promote globalization and meanwhile the local government should take measures to combat culture assimilation and sweat workshops This essay is extremely long (338 words instead of the advised 250-265) ... well-organized, and structure-wise the sentences are fine The vocabulary is impressive and there were only a few grammatical errors (see comments underlined in blue) Overall, this seems to be a band 7.5...
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Tài liệu Sexual and Reproductive Health of HIV Positive Women and Adolescent Girls: A Dialogue on Rights, Policies and Services ppt

Tài liệu Sexual and Reproductive Health of HIV Positive Women and Adolescent Girls: A Dialogue on Rights, Policies and Services ppt

Sức khỏe phụ nữ

... and the Ukraine iii) Summary of Main Issues Raised The overarching issue - at the root of barriers to care, lack of services and low quality of existing care - was the high degree of stigma and ... Discrimination and Rights Violations Stigmatizing and discriminatory attitudes toward HIV -positive women (and men) lie at the root of many human rights concerns and violations, and result in poor quality of ... rights and advocacy” and “SRH services and programmes and policies” - were focused on separately during consecutive months of the fora, themes of human rights violations in the context of SRH...
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx

Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx

Báo cáo khoa học

... pseudosubstrates and as reversible and irreversible inhibitors of EIIGlc and EIIMan Two assays were employed: (a) nonvectorial phosphorylation of Glc (analogues) by solubilized and purified EII and by EII-containing ... II-BGlc, a glucose receptor of the bacterial phosphotransferase system: molecular cloning of ptsG and purification of the receptor from an overproducing strain of Escherichia coli Proc Natl Acad Sci ... k2 ¼ 32 min)1, KI2 ¼ 700 lM KS1 and KS2 are the dissociation constants of E1 and E2 for Glc, KI1 and KI2 the dissociation constants for the inhibitor, and k1 and k2 are the turnover numbers DYNAFIT...
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Ergonomic Principles and Checklists for the Selection of Office Furniture and Equipment doc

Ergonomic Principles and Checklists for the Selection of Office Furniture and Equipment doc

Cao đẳng - Đại học

... Introduction Office Ergonomics and Occupational Health Principles for Designing an Office Workstation 4 Furniture and Equipment to be Selected Development and Use of Checklists 12 Try-outs and Consultation ... THE SELECTION OF OFFICE FURNITURE AND EQUIPMENT Introduction The Ergonomics Unit at Worksafe Australia receives frequent requests for advice on the purchase of furniture and equipment for offices ... furniture; # British Standard BS 5940:Part 1:1980, Office furniture, Part Specification for design and dimensions of office workstations, desks, tables and chairs; 12 # Health and Safety Executive,...
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Báo cáo khoa học: Dehydroepiandrosterone inhibits the proliferation and induces the death of HPV-positive and HPV-negative cervical cancer cells through an androgen- and estrogen-receptor independent mechanism pptx

Báo cáo khoa học: Dehydroepiandrosterone inhibits the proliferation and induces the death of HPV-positive and HPV-negative cervical cancer cells through an androgen- and estrogen-receptor independent mechanism pptx

Báo cáo khoa học

... estrogen and androgen, because use of antagonists to androgen and estrogen receptors (flutamide and ICI 182,780, respectively), and letrozol, an inhibitor of the aromatase responsible for converting androgen ... assess this, antagonists to androgen and estrogen receptors (flutamide and ICI 182,780, respectively), and an inhibitor of the aromatase responsible for conversion of androgen to estrogen (letrozol), ... associated with the presence and risk of development of cancer Oral administration of DHEA to mice inhibits spontaneous breast cancer and chemically induced tumors of the lung and colon [7]; however,...
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ON THE VALUATION OF GOODS AND SELECTION OF THE BEST DESIGN ALTERNATIVE docx

ON THE VALUATION OF GOODS AND SELECTION OF THE BEST DESIGN ALTERNATIVE docx

Tiếp thị - Bán hàng

... negative value of the slope of a cartel member's demand curve with price Res Eng Design 13 (2001) The basic assumption of the S-model is that demand is an analytic function of the N values and ... measure of the value of the product is also intuitive in the absence of the S-model The use of V±P as a measure of the driving force for demand was suggested independently by Anderson and Naurus ... weak in the type of market H E Cook, A Wu: On the valuation of goods and selection of the best design alternative Table Explanation of attribute indices studied here The EEVs of the lottery tickets...
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Request for Proposal For Selection of Project Management Unit (PMU) For Pension and Life Insurance Fund pot

Request for Proposal For Selection of Project Management Unit (PMU) For Pension and Life Insurance Fund pot

Quản lý dự án

... Approach and Methodology 20 Understanding Scope of Work 10 Approach and Methodology 10 C Profile of Key Team Members 30 34 Ministry of Overseas Indian Affairs Request for Proposal For Selection of ... consisting of two (2) copies of all the bid documents The name of the project, the bidder's name and address, and the name of the primary and secondary contact person should be provided on the right hand ... Proposal For Selection of Project Management Unit For Pension and Life Insurance Fund List of Acronyms MOIA Ministry of Overseas Indian Affairs POE Protectors of Emigrants PLIF Pension and Life Insurance...
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Financial Constraints, Endogenous Educational Choices And Self-selection Of Migrants doc

Financial Constraints, Endogenous Educational Choices And Self-selection Of Migrants doc

Khoa học xã hội

... assumption of homogeneity of degree of F (.) in K and H make the size of the firm in terms of number of workers L irrelevant for the firm’s maximization problem level k ∗ The profit maximization of the ... of migration B is positive: j = ⇔ B ≡ w1 (e) − w0 (e) − M (e) ≥ 3.2 (5) Selection We define selection on unobservables in terms of the skill parameter θ and selection on observables in terms of ... Educational Choices and Self -Selection of Migrants Juliano Assun¸ao c˜ Pontifical Catholic University of Rio de Janeiro juliano@econ.puc-rio.br Leandro Carvalho Rand carvalho@rand.org March 2010...
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Guide for the Selection of Chemical and Biological Decontamination Equipment for Emergency First Responders ppt

Guide for the Selection of Chemical and Biological Decontamination Equipment for Emergency First Responders ppt

Cao đẳng - Đại học

... Bureau of Justice Assistance, the Bureau of Justice Statistics, the Office of Juvenile Justice and Delinquency Prevention, and the Office for Victims of Crime U.S Department of Justice Office of ... LAW ENFORCEMENT AND CORRECTIONS STANDARDS AND TESTING PROGRAM The Law Enforcement and Corrections Standards and Testing Program is sponsored by the Office of Science and Technology of the National ... Coordination by: Office of Law Enforcement Standards National Institute of Standards and Technology Gaithersburg, MD 20899–8102 Prepared for: National Institute of Justice Office of Science and Technology...
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Guide for the Selection of Chemical and Biological Decontamination Equipment for Emergency First Responders pdf

Guide for the Selection of Chemical and Biological Decontamination Equipment for Emergency First Responders pdf

Cao đẳng - Đại học

... Bureau of Justice Assistance, the Bureau of Justice Statistics, the Office of Juvenile Justice and Delinquency Prevention, and the Office for Victims of Crime U.S Department of Justice Office of ... LAW ENFORCEMENT AND CORRECTIONS STANDARDS AND TESTING PROGRAM The Law Enforcement and Corrections Standards and Testing Program is sponsored by the Office of Science and Technology of the National ... Coordination by: Office of Law Enforcement Standards National Institute of Standards and Technology Gaithersburg, MD 20899 Prepared for: National Institute of Justice Office of Science and Technology...
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Báo cáo Y học: Selection of effective antisense oligodeoxynucleotides with a green fluorescent protein-based assay Discovery of selective and potent inhibitors of glutathione S-transferase Mu expression doc

Báo cáo Y học: Selection of effective antisense oligodeoxynucleotides with a green fluorescent protein-based assay Discovery of selective and potent inhibitors of glutathione S-transferase Mu expression doc

Báo cáo khoa học

... TRITC -positive; R4, GFP -positive; R5, TRITC -positive and GFP -positive (A–D) provide representive examples of multiple FACS analyses The table gives the amounts of cells (expressed as percentage of ... set of 15 PS-ODNs These ODNs selectively inhibit the expression of GSTM1 and/ or GSTM2 and can be used to study the influence of reduced GSTM expression on the detoxification of xenobiotics and ... by summation of the molecular weights of EGFP (25 kDa) and GSTM (27 kDa) Colocalization of ODN and pEGFP For proper evaluation of antisense effects, it is important that the AS-ODNs and the EGFP-expressing...
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Báo cáo Y học: Refolding of the Escherichia coli expressed extracellular domain of a7 nicotinic acetylcholine receptor Cys116 mutation diminishes aggregation and stabilizes the b structure pdf

Báo cáo Y học: Refolding of the Escherichia coli expressed extracellular domain of a7 nicotinic acetylcholine receptor Cys116 mutation diminishes aggregation and stabilizes the b structure pdf

Báo cáo khoa học

... a subunits of neuronal and muscle-type AChRs On the other hand, Cys116 is present only in a7 and a8 subunits We thought that the presence of a free SH group of Cys116 might be one of the factors ... flow rate of 0.5 mLÆmin)1 The same conditions, with the exception of presence or absence of detergents in the elution buffers, and designations apply to (B), (C) and (E) (B) GST–a7-(1–208) and GST–a7m-(1–208) ... has a 1.5-fold higher content of a helices and a somewhat higher percentage of unordered structure In view of the more stable secondary structure of the mutant and its decreased tendency to aggregation,...
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the influence of financial pressure on academic administrators' selection of management and resource allocation strategies

the influence of financial pressure on academic administrators' selection of management and resource allocation strategies

Kinh tế

... Reproduced with permission of the copyright owner Further reproduction prohibited without permission Reproduced with permission of the copyright owner Further reproduction prohibited ... without permission Reproduced with permission of the copyright owner Further reproduction prohibited without permission Reproduced with permission of the copyright owner Further reproduction prohibited ... without permission Reproduced with permission of the copyright owner Further reproduction prohibited without permission Reproduced with permission of the copyright owner Further reproduction prohibited...
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báo cáo hóa học:

báo cáo hóa học:" Determinants of Treatment Access in a Population-based Cohort of HIV-positive Men and Women Living in Argentina" pdf

Hóa học - Dầu khí

... briefly characterize the determinants of access to HAART and to assess late vs early initiation of HAART in a population-based cohort of HIV -positive Argentinean men and women Methods PUMA is an ongoing ... monitor access to and impact of HAART in Argentina using prospectively collected sociodemographic, clinical, and morbidity and mortality data for HIV -positive individuals 16 years and older, who ... considerable number of people initiated therapy late in the course of HIV disease Further characterization of the population of HIV -positive individuals eligible for HAART in Argentina, in terms of co-infections...
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Báo cáo hóa học:

Báo cáo hóa học: " Adaptive selection of antenna grouping and beamforming for MIMO systems" ppt

Hóa học - Dầu khí

... columns of the channel matrix are close to each other, and the channel is ill conditioned, and the performance of antenna grouping degrades When the sum of smaller two of (|A| + |F|), (|B| + |E|), and ... grouping algorithms and introduce mode selection criteria in Sections and The proposed antenna grouping algorithm and the mode selection criterion are presented in Sections 3.5 and 4.5, respectively ... Figures and 5, the performance of mode selection is the best By examining Figure and the condition number of the M4 algorithm, we can roughly obtain the desirable Figure Relationship of the condition...
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báo cáo hóa học:

báo cáo hóa học: " In vitro and in vivo pre-clinical analysis of a F(ab’)2 fragment of panitumumab for molecular imaging and therapy of HER1-positive cancers" pot

Hóa học - Dầu khí

... those of the F(ab’)2 fragment with a higher overall detection of tumor lesions The authors postulate that the success of these studies was the result of careful selection and matching of the ... μCi) of 86Y-CHX-A"-panitumumab F(ab’)2 and imaged and days post-injection of the RIC (c) Specificity was demonstrated by co-injecting 100 μg of panitumumab with the RIC and blocking uptake of the ... vitro and in vivo studies KEB and KG carried out radiolabelings TN performed PET imaging and analysis MWB synthesized the chelate and participated in the design of the studies DEM conceived of and...
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