cd45 cells prepared from synovium rich tissue
Báo cáo y học: "Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation" doc
... ductatCD11b+ subpopulations of transfer cells in synovium- rich tissue - indicated times after adoptive Phenotype of CD4+CD11b+ and CD 4cells MHC IIhi cells in synovium- rich tissue at indicated times after ... monocytelike cells are related to DCs Further investigation and enumeration of CD45+ subsets in synovium- rich tissue after adoptive transfer of arthritogenic thoracic duct cells Importantly, only CD45+ cells ... standard deviation, n = 5) (a) Numbers of total CD45+ , CD45+ MHC IIhi, CD45+ MHC IIlo, and CD45+ MHC II- cells in SRT prepared from normal rats and from rats to 14 days after adoptive transfer of...
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Báo cáo y học: "High CXCR3 expression in synovial mast cells associated with CXCL9 and CXCL10 expression in inflammatory synovial tissues of patients with rheumatoid arthritis" potx
... synovial tissue than in OA synovial tissue R246 It was previously found that T cells are present in approximately 50% of RA synovial tissue [42] According to our own observations, nearly 20% T cells ... expression of CXCR3 was detected in endothelial cells and dendritic cells, as well as in eosinophils within Th1 dominated tissues, including RA synovial tissue [19,26,27] Thus, CXCR3 expression does ... found in synovial tissue from RA as compared with that from OA patients Concomitantly, significantly increased mRNA levels of CXCL9 and CXCL10 were also detected in RA synovial tissue Our immunohistochemical...
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báo cáo khoa học: "Prognostic significance of STAT3 and phosphorylated STAT3 in human soft tissue tumors - a clinicopathological analysis" ppt
... endothelial cells lining tiny rounded vascular spaces with inconspicuous and (D) shows proliferation of spindle cells in inflammatory background The malignant soft tissue tumors (E) shows epithelioid cells ... and intermediate grade soft tissue tumors compared to benign tumors Lane 1: malignant soft tissue tumor; lane 2: intermediate soft tissue tumor; lane 3: benign soft tissue tumor b-actin was used ... different stages of soft tissue tumors v/s GAPDH (269 bp) (A and B) Lane 1: benign soft tissue tumor; lane 2: intermediate soft tissue tumor; lane 3: malignant soft tissue tumor A 100-bp ladder...
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báo cáo khoa học: " Conifer R2R3-MYB transcription factors: sequence analyses and gene expression in wood-forming tissues of white spruce (Picea glauca)" pot
... as bark tissues were collected from three 30–40 cm bolts taken from the lower third of the main stem These vascular tissues were scraped with a scalpel immediately after peeling the bark Tissues ... various organs and tissues Transcript Transcript abundance for 13 spruce MYB genes and 4CL in various organs and tissues Transcript abundance was determined by Q-RTPCR of six tissues from two different ... and RNA isolation Several tissues were isolated from two 33-year-old P glauca trees felled in July 2003, from a progeny trial established near Quebec City (Canada) All tissues were frozen in liquid...
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Báo cáo sinh học: "Genetic and metabolic aspects of androstenone and skatole deposition in pig adipose tissue: A review (Open Access publication)" potx
... Indoles are produced by bacteria in the colon, from the breakdown of the amino-acid tryptophan [18] The reason why high levels of skatole in fat tissue exist in some intact pig males, but not ... is high (h2 = 0.56), ranging from 0.25 to 0.88 [35] with recent estimated values in the same range [36, 48] Skatole levels show medium heritability values ranging from 0.19 to 0.54 [31, 45] In ... (5 α-androst-16en-3-one) is synthesized in the testis from pregnenolone [16,19,20], in relation with sexual development, and stored in fat tissue because of its lipophilic properties The pathways...
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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
... treatments Primary cultures of SMCs were prepared from the bladders of mid- to late-gestational fetal calves as previously described [12,18] Freshly isolated cells were phenotypically characterized ... and myosin Cells were maintained in DMEM supplemented with 10% (v/v) fetal bovine serum (FBS) and antibiotics in a humidified atmosphere containing 5% (v/v) CO2 in air at 37 °C Cells from passages ... an additional h RNA isolation and northern blot analysis Total RNA was extracted from cells using TRIzol Reagent from Invitrogen A sample containing 12 lg of total RNA was fractionated by electrophoresis...
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Ambient particulate air pollution induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues ppt
... cells in WAT These so called “brite” cells are present in WAT as evidenced by the presence of UCP1 expressing cells in WAT Studies in cell culture indicate that brown adipocytes and muscle cells ... at least two distinct types of adipose cells, white and brown adipocytes, with opposing effects on energy balance and body weight regulation White adipose tissue (WAT) is highly adapted to store ... demonstrated that brown and white adipocytes are not sister cells, but rather that brown adipocytes are closely related to myocytes, and both originate from a common “adipomyocyte” precursor [4,5] Among...
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Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc
... detected in KM12, KM20L2 and WiDr cells COX-1 was highly expressed in HT29 and WiDr cells and weakly expressed in KM20L2 cells COX-2 was detected only in WiDr cells The two terminal PGE2-biosynthetic ... [41] RT-PCR analyses revealed that, unlike mouse mast cells, human mast cells developed in the presence of SCF and IL-6 from cord blood cells [38] expressed only sPLA2-IID, but not the other ... analysis has demonstrated that human intestinal mast cells contain sPLA2-IIA [55] We also recently found that sPLA2-V is located in mast cells in tissues from patients with allergic symptoms (M Murakami...
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Báo cáo khoa học: Increased glucose metabolism and ATP level in brain tissue of Huntington’s disease transgenic mice pdf
... 2) Cellfree extracts were prepared from the affected (posterior) and unaffected (anterior and cerebellum) regions of the HD, from the neurotoxin-administered as well as from the control mice In ... studies were performed with brain tissues containing different types of neuronal cells as well as a large excess of glial cells Astrocytes, a sub-type of the glial cells (outnumbering the neurones ... increased by 30% Vmax (GAPDH) two-fold increase and KmGAP (GAPDH) from 20 to lM KmGAP (GAPDH) from 20 to lM KmGAP (aldolase) from 300 to 75 lM 6a and 5a 6b and 5b FEBS Journal 275 (2008) 4740–4755...
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Báo cáo Y học: Expression of the aspartate/glutamate mitochondrial carriers aralar1 and citrin during development and in adult rat tissues docx
... blot analysis of aralar1 and citrin in cells from the immune system and hematopoietic tissues Mitochondrial extracts (20 lg) obtained from human Jurkat cells (a T-cell leukemia cell line) and ... fractions from a few representative tissues were processed in parallel with either known amounts of recombinant citrin [7] and aralar1 [6] or mitochondrial fractions from HEK-293T cells overexpressing ... analysis Total RNA was extracted from rat tissues using the guanidine isothiocyanate method Northern blot analysis was carried out using 20 lg total RNA from different rat tissues as previously described...
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báo cáo hóa học:" Aberrant STYK1 expression in ovarian cancer tissues and cell lines" pdf
... estradiol-treated cells although the increase was only significant in OvCar5 cells (p < 0.001; Fig 3B) Conversely, STYK1 expression decreased slightly in G-1-treated OvCar8 cells When the cells were ... using the 2ΔΔCT formula [16] RNA was analyzed from three independent experiments Western blotting Lysates were collected from treated and untreated cells in modified radioimmuno precipitation assay ... Formalin-fixed arrays of normal, benign, and malignant ovarian tissues were obtained from Pantomics Inc (San Franscico, CA) The tissues were stained with a mouse monoclonal antibody for STYK1...
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Báo cáo hóa học: " Effect of annealing treatments on photoluminescence and charge storage mechanism in silicon-rich SiNx:H films" docx
... are silicon rich, being characterized by excess of Si ranging from 50 to 20 at.% in this study As evidenced from ERDA measurements, the total amount of hydrogen content decreases from 16 to at.% ... strong luminescence from SiNx materials is commonly suggested from the combination of Si/SiNx interface luminescence, gap state luminescence/band-tail luminescence, and luminescence from Si nanodots/clusters ... characteristics of silicon -rich silicon nitride thin films Jpn J Appl Phys 2007, 46:3206 19 Vamvakas VEm, Gardelis S: FTIR characterization of light emitting Si -rich nitride films prepared by low pressure...
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Báo cáo lâm nghiệp: "Effects of defoliation on the frost hardiness and the concentrations of soluble sugars and cyclitols in the bark tissue of pedunculate oak (Quercus robur L.)" potx
... previous measurements had shown that, in bark tissue from oaks, a linear relationship exists between the index of injury and freezing temperatures ranging from –5 °C to –30 °C [45, 46] Second, temperatures ... dehardening of bark tissue from oaks during winter can be revealed [44, 46] In order to test whether the actual air temperatures during winter did cause any injury to the bark tissue of the trees, ... SD treatment did not differ significantly from that of control or RD trees Therefore, chemical analyses of the bark tissue were confined to samples from control and repeatedly defoliated oaks...
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Báo cáo toán học: "Vertex subsets with minimal width and dual width in Q-polynomial distance-regular graphs" docx
... matroid [37] The semilattice structure of Γ is then completely recovered from P, and the characterization of Γ follows from the classification of nontrivial regular quantum matroids with rank at ... Chapter 3] for terminology from poset theory the electronic journal of combinatorics 18 (2011), #P167 13 We now list five examples of nontrivial regular quantum matroids from [37] In (7.1)– (7.5) ... (A.1) is taken from [40], but the presentation is changed so as to be consistent with the notation in [2, 34, 35, 36] (A.1) Theorem([40, Theorem 5.16]) Let Φ be the Leonard system from (3.1) and...
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Báo cáo y học: "Expression and functional properties of antibodies to tissue inhibitors of metalloproteinases (TIMPs) in rheumatoid arthritis" docx
... Antibodies from RA patients support metalloproteinase activity by neutralizing TIMP-2 The purified IgG fractions originating from two RA serum samples containing antibodies against TIMPs and from two ... citrate (0.129 mol/l; pH 7.4) All synovial fluid samples were obtained from knee joints At the same time, blood samples were obtained from the cubital vein and directly transferred into sodium citrate ... was low and ranged from 0% to 6% The samples of the control groups within SD demonstrated a bimodal distribution Purification of immunoglobulins Four serum samples were selected from RA patients,...
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Báo cáo y học: "Enumeration and phenotypical analysis of distinct dendritic cell subsets in psoriatic arthritis and rheumatoid arthritis" pps
... was obtained from patients with RA (n = 12), from patients with PsA (n = 13) and from patients with OA (n = 11), and was compared with healthy controls (n = 12) SF was obtained from a subset ... number of cells (×104/ml) in rheumatoid arthritis and psoriatic arthritis peripheral blood and matched synovial fluid White blood cells Myeloid dendritic cells Plasmacytoid dendritic cells Rheumatoid ... sufficient to induce substantial IL-10 release from healthy PB mDCs and pDCs, it was sufficient for IL-10 release from PsA SF and RA SF purified cells plasma cells, immature DCs are able to capture...
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Báo cáo y học: "Nurse-like cells reside in the synovial tissue and bone marrow in rheumatoid arthritis" doc
... that of FSCs derived from synovial tissue and bone marrow cells from non-RA controls Namely, the phenotype of NLCs derived from osteoarthritis patients and human skin nurse cells was similar to ... 10,615 540 355 255 RA-BMNCs, cytokine production from RA-NLCs derived from bone marrow cells; RA-SNCs, cytokine production from RA-NLCs derived from synovium; G-CSF, granulocyte colony-stimulating ... production from RA-NLCs derived from synovium and immunoglobulin from B cellsb [6] RA-SNCs
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