capillary electrophoresis of biopharmaceutical proteins
capillary electrophoresis of carbohydrates
... Profiling of oligosaccharidemediated microheterogeneity of a monoclonal antibody by capillary electrophoresis Electrophoresis 17, 418–422 58 Kakehi, K and Honda, S (1996) Analysis of glycoproteins, ... speculation about the purpose of GPI anchors in terms of membrane mobility, role or lack thereof in signaling, and in the controlled release of proteins, the functions of this mode of attachment remain ... silica capillary is slow under these conditions Introduction of ABEE derivatives from the anodic end of the capillary will Derivation of Carbohydrates 49 Fig Capillary electrophoretic analysis of...
- 297
- 321
- 0
capillary electrophoresis of nucleic acids, volume i
... constant denaturant capillary gel electrophoresis CGE, non-denaturing capillary gel electrophoresis CEMSA, capillary electrophoresis mobility shift assay CE/MS, capillary electrophoresis/ mass ... sieving capacity Electrophoresis 19, 3154–3159 19 Chiari, M and Cretich, M (2001) Capillary coatings: choices for capillary electrophoresis of DNA, in Capillary Electrophoresis of Nucleic Acids, ... sequencing by capillary electrophoresis Electrophoresis 18, 2393–2399 42 Dovichi, N J and Zhang, J.-Z (2001) DNA sequencing by capillary array electrophoresis, in Capillary Electrophoresis of Nucleic...
- 456
- 377
- 0
capillary electrophoresis of nucleic acids, volume ii
... the fast electrophoresis protocol consisted of a capillary of 41 cm total length, an electrophoresis voltage of 15 kV and 2% GeneScan polymer in 0.6X electrophoresis buffer and 60°C electrophoresis ... for a number of applications in DNA analysis The From: Methods in Molecular Biology, Vol 163: Capillary Electrophoresis of Nucleic Acids, Vol 2: Practical Applications of Capillary Electrophoresis ... the same, regardless of the type of the separation channel used (capillary or chip) The main advantage of the chip technology is the ease of fabrication of a large number of channels In comparison...
- 365
- 528
- 0
Báo cáo khoa học: "Two-dimensional gel electrophoresis of membrane proteins from ectomycorrhizal fungi" doc
... likely that some of the SR -proteins are membrane-bound proteins involved in recognition, metabolite transport and protein secretion However, investigation of the protein composition of symbiotic ... with separate lots of samples min) Method After solubilisation of membrane proteins corresponding to 300 mg fresh weight by 300 μl of buffer as described in method 1, four vol of cold (-20 °C) ... plasma membrane, and component of the protein secretory pathway are therefore present in this microsomal fraction Solubilisation of membrane proteins Membrane proteins of P tinctorius and C geophilum...
- 11
- 115
- 0
Báo cáo Y học: Characterization of heparin binding by a peptide from amyloid P component using capillary electrophoresis, surface plasmon resonance and isothermal titration calorimetry ppt
... at a capillary temperature thermostatting of 20 °C The capillary was rinsed after electrophoresis for with each of the following: 0.1 M NaOH, water, and electrophoresis buffer Samples were pressure ... fragmentation pattern of the two N-terminal amino-acid residues of mAP-1 supported this notion (data not shown) Fig Heparin binding specificity of synthetic AP-1 peptides in capillary electrophoresis ... constant amount of LMW heparin or water (controls) Thus, the differences in the positions of the fitted straight lines are indicative of the amount of bound peptide It is clear that binding of heparin...
- 8
- 347
- 0
clinical applications of capillary electrophoresis
... quantitation of human urine proteins by capillary electrophoresis J Chromatogr B 662, 108–112 39 Jenkins, M A (1997) Clinical application of capillary electrophoresis to unconcentrated human urine proteins ... Quantitative analysis of serum proteins separated by capillary electrophoresis Clin Chem 39,689–692 Jenkins, M A and Guerin, M D (1995) Quantification of serum proteins using capillary electrophoresis ... (1995) Capillary zone electrophoresis of serum proteins: study of separation variables J Chromatogr A 709, 99–110 10 Lehmann, R., Liebich, H M., and Voelter, W (1996) Application of capillary electrophoresis...
- 200
- 204
- 0
Báo cáo khoa học: "Contribution of two-dimensional electrophoresis of proteins to maritime pine genetics" ppt
... Two-dimensional electrophoresis of denatured proteins (2D PAGE) allows the analysis of several hundreds of gene products in a single gel (O’Farrell, 1975) In gymnosperm species proteins have been ... explained by the existence of major genes responsible for the determinism of polypeptide amounts A total of 38% of the proteins were clustered at two loci Each group of covariable spots could ... twodimensional gel electrophoresis of needle, bud and pollen proteins J Mol Evol 41, 231-237 Bahrman N, Zivy M, Damerval C, Baradat P (1994) Organisation of the variability of abundant proteins in seven...
- 12
- 400
- 0
báo cáo khoa học: "Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay" docx
... to the attempt of bioconjugating QD-COOH to proteins of increasingly higher pI, using cationic proteins such as cytochrome c and lysozyme However, it was observed that the pI of proteins can play ... Separations of chemically different particles by capillary electrophoresis Anal Chem 1990, 62:2484-2490 Cifuentes A, Rodriguez MA, García-Montelongo FJ: Separation of basic proteins in free solution capillary ... Dolník V: Applications of capillary electrophoresis on microchip Electrophoresis 2006, 28(15):1094-2009 Baryla NE, Lucy CA: Simultaneous separation of cationic and anionic proteins using zwitterionic...
- 15
- 301
- 0
The development of acidic protein aptamers using capillary electrophoresis methods and their use in surface plasmon resonance
... positions of the constituents of the equilibrium in the capillary at t0 and t1; (c) electrophoretogram plot profile of the equilibrium mixture with the area of the unbound DNA library (A1), the area of ... efficiency of selection CE-SELEX takes advantage of the higher separation efficiency of capillary electrophoresis and does not require the target to be immobilized71 CE-SELEX involves the use of capillary ... positions of the constituents of the equilibrium in the capillary at t0 and t1; (c) electrophoretogram plot profile of the equilibrium mixture with the area of the unbound DNA library (A1), the area of...
- 205
- 284
- 0
Novel methods for quantitative analysis and evaluation of effects of chronic exposure to microcystins by capillary electrophoresis and metabolomic studies
... Figure 8: Illustration of EOF inside a fused silica capillary 22 Figure 9: Illustration of the order of migration of ions in CE 22 Figure 10 Illustration of EOF profile 23 Figure ... schematic diagram of a 15 Figure 4: Schematic diagram of capillary electrophoresis system High performance capillary electrophoresis (HPCE) does not require the use of gels because the capillary walls ... EVALUATION OF EFFECTS OF CHRONIC EXPOSURE TO MICROCYSTINS BY CAPILLARY ELECTROPHORESIS AND METABOLOMIC STUDIES GRACE BIRUNGI (MSc) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF...
- 177
- 292
- 0
Applications of capillary electrophoresis in the analysis of natural products
... coating capillary wall (hydrophillicity or charge) Stability often problematic Table 1.2 Different Modes of High Performance Capillary Electrophoresis CE Mode Capillary zone electrophoresis (CZE) Capillary ... migration of cations results in the migration of whole buffer solution through the capillary; this migration is the EOF Since the driving force of EOF is uniformly distributed along the capillary ... perspective of Capillary Electrophoresis Since the introduction of capillary electrophoresis (CE) in 1980s by Jorgenson, the good performance and relatively simple instrumentation quickly make CE one of...
- 155
- 525
- 0
Development of methods to improve sensitivity and portability of capillary electrophoresis for the analysis of stabilizers and drugs 1
... Basic Theory of Capillary Electrophoresis ……………………….… 1~6 1.2 Electroosmotic Flow (EOF)…………………………………………… 6~13 1.3 Different Modes of CE…………………………………………….…… 13~20 1.3.1 Capillary Zone Electrophoresis ... Cyclodextrin CE Capillary Electrophoresis CEC Capillary Electrochromatography CF Concentration factor CF-FAB Continuous-flow fast atom bombardment CGE Capillary gel electrophoresis CIEF Capillary isoelectric ... 1.3.3 Capillary Gel Electrophoresis (CGE)………………………… 15~16 1.3.4 Capillary Isoelectric Focusing (CIEF)……………………….… 16~17 1.3.5 Capillary Electrochromatography (CGE)……………………… 17~18 1.3.6 Capillary...
- 16
- 283
- 0
Development of methods to improve sensitivity and portability of capillary electrophoresis for the analysis of stabilizers and drugs 2
... analysis 12 Figure 1.6 Illustration of double layer and reversal of EOF by addition of a quaternary amine to the buffer 1.3 Different Modes of CE 1.3.1 Capillary Zone Electrophoresis (CZE) CZE, which ... separation of immunoglobulins, hemoglobins and the measurement of the pI of proteins, 1.3.5 Capillary Electrochromatography (CEC) Figure 1.10 Scheme of CEC mode CEC is based on the partitioning of analytes ... Landers, Handbook of Capillary Electrophoresis, USA, 1996 [5] R Kuhn, S H Kuhn, Capillary Electrophoresis: Principles and Practice, Berlin, 1993 [6] P G Righetti, Capillary Electrophoresis in...
- 160
- 1,228
- 0
Development and application of novel capillary electrophoresis techniques for analysis of DNA fragments and organic pollutants
... effects of pollutants at biological level XVI List of Tables List of Tables Table 1.1 Methods to Control EOF…………………… …… Table 1.2 Different Modes of Capillary Electrophoresis … Table 1.3 LODs of ... Concentration of MWCN .92 XXI List of Figures Figure 3.11 Electrophoresis of ΦX174 DNA by CE-CCD in Buffers containing Different Concentration of MWCN .94 Figure 3.12 Electrophoresis of 2-Log ... Chapter Introduction 1.1 Overview of Capillary Electrophoresis Since the modern era of capillary electrophoresis (CE) commenced in the early 1980s with a series of publications by Jorgenson and...
- 223
- 752
- 0
Analysis of intact bacteria, bacterial DNA and mutagenic alkaloids by capillary electrophoresis
... Table of Contents Acknowledgements I Table of Contents II Summary VII Chapter Introduction 1.1 Electrophoresis 1.2 History of capillary electrophoresis 1.3 Basic principles of capillary electrophoresis ... techniques Figure 1-3 Flow profiles of EOF and laminar flow 1.3.3 Measurement of EOF Routine measurement of the EOF is necessary to ensure the integrity of the separation If the EOF is not reproducible, ... conductivity in the vicinity of the adsorbed polymer particles suggest a possible focusing or compaction mechanism as shown in Figure 2-1 A EOF EOF B EOF EOF C EOF EOF D EOF EOF Figure 2-1 Hairy particle...
- 173
- 380
- 0
Synthesis of ionic liquids and their applications in capillary electrophoresis
... by Vigh et al [74] for weak EOF Fig 1-4 Comparison of flow profiles of chromatography and CE 1.2.3.1.1 Control of EOF It is desirable to manipulate the magnitude of EOF in order to optimize the ... grape They found that EOF of the capillary was reversed by adsorption of the IL cations onto the silica wall, and the magnitude of the anodic EOF increased with the amount of IL added The neutral ... migration direction of anion, cation and EOF in a fused silica capillary The magnitude of the EOF depends on the surface concentration of silanol groups and on their degree of dissociation The...
- 201
- 474
- 0
Use of Cyanobacterial Proteins to Engineer New Crops
... biofarming is a major goal of plant biotechnology Direct transfer of plant genes has resulted in new varieties with improved properties However, this approach is limited by the genetic stock of ... the various existing Use of Cyanobacterial Proteins 67 Fig 4.1 Summary of current advances in plant biotechnology using cyanobacterial genes Gene mining on cyanobacteria offers a promising opportunity ... phytochrome reaction spectra Production of biodegradable polymers (Section 4.5) Phytochrome perception (Section 4.6) (synthesis of PCB) Biofarming Production of cyanophycin Pigment manipulation (Section...
- 6
- 479
- 0
Tài liệu Báo cáo khoa học: Seeking the determinants of the elusive functions of Sco proteins pptx
... divergence of Sco proteins in prokaryotes is apparent from the analysis of their genomes, Determinants of the elusive functions of Sco proteins some of which contain genes coding for Sco proteins ... variety of possible functions Given that the fold of Sco proteins resembles that of proteins involved in thiol-disulfide redox chemistry, it is reasonable to assume that the primary role of Sco proteins ... oxidoreductase function of the CXXXC motif of human Sco proteins could therefore be implicated not only in the maturation of the CuA site of Cox2 but also in the maintenance of cellular copper homeostasis...
- 19
- 743
- 0
Tài liệu Báo cáo khoa học: The social life of ribosomal proteins doc
... functional effects of these mutations were due to the architectural and stabilizing role of the proteins alone, i.e that perturbation of protein structure would affect the catalytic activity of the rRNA ... composed of RNA However, two long C-terminal tails of small ribosomal subunit proteins S9 and S13 that are otherwise located at the top of the 30S, make their way down through the head of the subunit ... antiterminator of transcription of k phage N protein in E coli [58] Furthermore, several ribosomal proteins are known to regulate the expression of themselves or other ribosomal proteins through...
- 11
- 563
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008