capillary electrophoresis of bacterial lipo polysaccharides
capillary electrophoresis of carbohydrates
... (1996) Profiling of oligosaccharidemediated microheterogeneity of a monoclonal antibody by capillary electrophoresis Electrophoresis 17, 418–422 58 Kakehi, K and Honda, S (1996) Analysis of glycoproteins, ... silica capillary is slow under these conditions Introduction of ABEE derivatives from the anodic end of the capillary will Derivation of Carbohydrates 49 Fig Capillary electrophoretic analysis of ... speculation about the purpose of GPI anchors in terms of membrane mobility, role or lack thereof in signaling, and in the controlled release of proteins, the functions of this mode of attachment remain...
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capillary electrophoresis of nucleic acids, volume i
... constant denaturant capillary gel electrophoresis CGE, non-denaturing capillary gel electrophoresis CEMSA, capillary electrophoresis mobility shift assay CE/MS, capillary electrophoresis/ mass ... sieving capacity Electrophoresis 19, 3154–3159 19 Chiari, M and Cretich, M (2001) Capillary coatings: choices for capillary electrophoresis of DNA, in Capillary Electrophoresis of Nucleic Acids, ... sequencing by capillary electrophoresis Electrophoresis 18, 2393–2399 42 Dovichi, N J and Zhang, J.-Z (2001) DNA sequencing by capillary array electrophoresis, in Capillary Electrophoresis of Nucleic...
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capillary electrophoresis of nucleic acids, volume ii
... the fast electrophoresis protocol consisted of a capillary of 41 cm total length, an electrophoresis voltage of 15 kV and 2% GeneScan polymer in 0.6X electrophoresis buffer and 60°C electrophoresis ... for a number of applications in DNA analysis The From: Methods in Molecular Biology, Vol 163: Capillary Electrophoresis of Nucleic Acids, Vol 2: Practical Applications of Capillary Electrophoresis ... the same, regardless of the type of the separation channel used (capillary or chip) The main advantage of the chip technology is the ease of fabrication of a large number of channels In comparison...
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Analysis of intact bacteria, bacterial DNA and mutagenic alkaloids by capillary electrophoresis
... Table of Contents Acknowledgements I Table of Contents II Summary VII Chapter Introduction 1.1 Electrophoresis 1.2 History of capillary electrophoresis 1.3 Basic principles of capillary electrophoresis ... techniques Figure 1-3 Flow profiles of EOF and laminar flow 1.3.3 Measurement of EOF Routine measurement of the EOF is necessary to ensure the integrity of the separation If the EOF is not reproducible, ... conductivity in the vicinity of the adsorbed polymer particles suggest a possible focusing or compaction mechanism as shown in Figure 2-1 A EOF EOF B EOF EOF C EOF EOF D EOF EOF Figure 2-1 Hairy particle...
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Báo cáo Y học: Characterization of heparin binding by a peptide from amyloid P component using capillary electrophoresis, surface plasmon resonance and isothermal titration calorimetry ppt
... at a capillary temperature thermostatting of 20 °C The capillary was rinsed after electrophoresis for with each of the following: 0.1 M NaOH, water, and electrophoresis buffer Samples were pressure ... fragmentation pattern of the two N-terminal amino-acid residues of mAP-1 supported this notion (data not shown) Fig Heparin binding specificity of synthetic AP-1 peptides in capillary electrophoresis ... constant amount of LMW heparin or water (controls) Thus, the differences in the positions of the fitted straight lines are indicative of the amount of bound peptide It is clear that binding of heparin...
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clinical applications of capillary electrophoresis
... Characterization of lipoproptein a by capillary zone electrophoresis J Chromatogr A 717(1–2), 33–39 29 Holmes, R P (1995) Measurement of urinary oxalate and citrate by capillary electrophoresis ... (1995) Capillary zone electrophoresis of serum proteins: study of separation variables J Chromatogr A 709, 99–110 10 Lehmann, R., Liebich, H M., and Voelter, W (1996) Application of capillary electrophoresis ... applications of capillary electrophoresis Ann Clin Lab Sci 22, 398–405 Jenkins, M A and Guerin M D (1996) Capillary electrophoresis as a clinical tool J Chromatogr B 682, 23–34 Clinical Applications of...
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báo cáo khoa học: "Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay" docx
... Separation of bioconjugated quantum dots using capillary electrophoresis Anal Chem 2008, 80:1988-1994 Pereira M, Lai EPC, Hollebone B: Characterization of quantum dots using capillary zone electrophoresis ... net-charge of the conjugate The increase in peak width of the QD-BSA can be attributed to a number of factors, including the polydispersity Page of 15 (page number not for citation purposes) Journal of ... particles by capillary zone electrophoresis Electrophoresis 2002, 23:1957-1972 Quang C, Petersen SL, Ducatte GR, Ballou NE, et al.: Characterization and separation of inorganic fine particles by capillary...
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The development of acidic protein aptamers using capillary electrophoresis methods and their use in surface plasmon resonance
... positions of the constituents of the equilibrium in the capillary at t0 and t1; (c) electrophoretogram plot profile of the equilibrium mixture with the area of the unbound DNA library (A1), the area of ... efficiency of selection CE-SELEX takes advantage of the higher separation efficiency of capillary electrophoresis and does not require the target to be immobilized71 CE-SELEX involves the use of capillary ... positions of the constituents of the equilibrium in the capillary at t0 and t1; (c) electrophoretogram plot profile of the equilibrium mixture with the area of the unbound DNA library (A1), the area of...
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Novel methods for quantitative analysis and evaluation of effects of chronic exposure to microcystins by capillary electrophoresis and metabolomic studies
... Figure 8: Illustration of EOF inside a fused silica capillary 22 Figure 9: Illustration of the order of migration of ions in CE 22 Figure 10 Illustration of EOF profile 23 Figure ... schematic diagram of a 15 Figure 4: Schematic diagram of capillary electrophoresis system High performance capillary electrophoresis (HPCE) does not require the use of gels because the capillary walls ... EVALUATION OF EFFECTS OF CHRONIC EXPOSURE TO MICROCYSTINS BY CAPILLARY ELECTROPHORESIS AND METABOLOMIC STUDIES GRACE BIRUNGI (MSc) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF...
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Applications of capillary electrophoresis in the analysis of natural products
... coating capillary wall (hydrophillicity or charge) Stability often problematic Table 1.2 Different Modes of High Performance Capillary Electrophoresis CE Mode Capillary zone electrophoresis (CZE) Capillary ... migration of cations results in the migration of whole buffer solution through the capillary; this migration is the EOF Since the driving force of EOF is uniformly distributed along the capillary ... perspective of Capillary Electrophoresis Since the introduction of capillary electrophoresis (CE) in 1980s by Jorgenson, the good performance and relatively simple instrumentation quickly make CE one of...
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Development of methods to improve sensitivity and portability of capillary electrophoresis for the analysis of stabilizers and drugs 1
... Basic Theory of Capillary Electrophoresis ……………………….… 1~6 1.2 Electroosmotic Flow (EOF)…………………………………………… 6~13 1.3 Different Modes of CE…………………………………………….…… 13~20 1.3.1 Capillary Zone Electrophoresis ... Cyclodextrin CE Capillary Electrophoresis CEC Capillary Electrochromatography CF Concentration factor CF-FAB Continuous-flow fast atom bombardment CGE Capillary gel electrophoresis CIEF Capillary isoelectric ... 1.3.3 Capillary Gel Electrophoresis (CGE)………………………… 15~16 1.3.4 Capillary Isoelectric Focusing (CIEF)……………………….… 16~17 1.3.5 Capillary Electrochromatography (CGE)……………………… 17~18 1.3.6 Capillary...
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Development of methods to improve sensitivity and portability of capillary electrophoresis for the analysis of stabilizers and drugs 2
... analysis 12 Figure 1.6 Illustration of double layer and reversal of EOF by addition of a quaternary amine to the buffer 1.3 Different Modes of CE 1.3.1 Capillary Zone Electrophoresis (CZE) CZE, which ... Landers, Handbook of Capillary Electrophoresis, USA, 1996 [5] R Kuhn, S H Kuhn, Capillary Electrophoresis: Principles and Practice, Berlin, 1993 [6] P G Righetti, Capillary Electrophoresis in ... separation of immunoglobulins, hemoglobins and the measurement of the pI of proteins, 1.3.5 Capillary Electrochromatography (CEC) Figure 1.10 Scheme of CEC mode CEC is based on the partitioning of analytes...
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Development and application of novel capillary electrophoresis techniques for analysis of DNA fragments and organic pollutants
... effects of pollutants at biological level XVI List of Tables List of Tables Table 1.1 Methods to Control EOF…………………… …… Table 1.2 Different Modes of Capillary Electrophoresis … Table 1.3 LODs of ... Concentration of MWCN .92 XXI List of Figures Figure 3.11 Electrophoresis of ΦX174 DNA by CE-CCD in Buffers containing Different Concentration of MWCN .94 Figure 3.12 Electrophoresis of 2-Log ... Chapter Introduction 1.1 Overview of Capillary Electrophoresis Since the modern era of capillary electrophoresis (CE) commenced in the early 1980s with a series of publications by Jorgenson and...
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Synthesis of ionic liquids and their applications in capillary electrophoresis
... by Vigh et al [74] for weak EOF Fig 1-4 Comparison of flow profiles of chromatography and CE 1.2.3.1.1 Control of EOF It is desirable to manipulate the magnitude of EOF in order to optimize the ... grape They found that EOF of the capillary was reversed by adsorption of the IL cations onto the silica wall, and the magnitude of the anodic EOF increased with the amount of IL added The neutral ... migration direction of anion, cation and EOF in a fused silica capillary The magnitude of the EOF depends on the surface concentration of silanol groups and on their degree of dissociation The...
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Tài liệu Báo cáo khoa học: Understanding the binding properties of an unusual metal-binding protein ) a study of bacterial frataxin pdf
... Superimpositions of a reference [1H,15N] HSQC spectrum of CyaY (blue) with a spectrum (red) of a : mixture of CyaY ⁄ Gd3+ (A), a : mixture of CyaY ⁄ Lu3+ (B), and a : mixture of CyaY ⁄ Ca2+ (C) ... concomitant appearance of other signals in other parts of the spectrum This result could be a consequence of the paramagnetic properties of Fe(II), or it might arise from the presence of an intermediate ... of the experiment, we added to the solution five equivalents of Ca2+ As previously noted, the presence of Ca2+ induced small shifts, among which is the diagnostic downfield shift of the amide of...
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Tài liệu Báo cáo khoa học: Phylogenetic relationships in class I of the superfamily of bacterial, fungal, and plant peroxidases pptx
... alignment of 50 selected representatives of the superfamily of bacterial, fungal and plant heme peroxidases: region on the proximal side of the prosthetic heme group Abbreviations of enzyme sources ... conserved in all known C-domains of ´ 3304 M Zamocky (Eur J Biochem 271) ´ Ó FEBS 2004 Fig Structural comparison of four representatives of class I of the superfamily of bacterial, fungal, and plant ... FEBS 2004 Molecular evolution of heme peroxidases (Eur J Biochem 271) 3303 Fig Multiple sequence alignment of 50 selected representatives of the superfamily of bacterial, fungal and plant heme...
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Báo cáo khoa học: Concepts and tools to exploit the potential of bacterial inclusion bodies in protein science and biotechnology pdf
... technique of choice also in the study of the influence of the physiology of expression (i.e temperature, induction, formation of disulfide bonds) on the kinetics of aggregation and on the structure of ... [57,61] Potential of bacterial inclusion bodies Fig FTIR analysis of the aggregation of a recombinant protein in E coli (A) Second derivatives of the FTIR absorption spectra of E coli cells synthesizing ... intermolecular b-sheets The use of this spectroscopic technique for the study of IB aggregates is often limited by the intrinsic insolubility of the samples, responsible for a high level of light scattering...
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Báo cáo khoa học: Protein aggregation and amyloid fibril formation prediction software from primary sequence: towards controlling the formation of bacterial inclusion bodies pot
... correctly predicts 80% of amyloidogenic peptides (115 of 144 peptides) and 72% of non-amyloidogenic ones (189 of 263 peptides), with a cut-off value of 0.062, from a database of 407 amyloidogenic ... FEBS 2433 Software for controlling formation of bacterial inclusion bodies S J Hamodrakas The testing of ‘anti-amyloid’ drugs that would prevent the formation of bacterial IBs in bacterial cell ... cut-off (have numbers of expected contacts higher than the cut-off) and the size of the region is greater than or equal to the size of the sliding window used Optimum values for the cut-off (threshold)...
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Báo cáo khoa học: Biological role of bacterial inclusion bodies: a model for amyloid aggregation potx
... the discovery of the reversibility of IB formation [6], the general acceptance of IBs being formed by functional proteins [7] and the recognition of the amyloid-like architecture of IB proteins ... and timely analysis of the amyloid architecture of IB proteins [14,15] has led to the use of these underestimated bacterial aggregates as intriguing models for the analysis of protein–protein ... U (2004) Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins Microb Cell Fact 3, 11–22 Biological role of bacterial inclusion bodies Carrio MM...
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Báo cáo khoa học: Structure of the O polysaccharides and serological classification of Pseudomonas syringae pv. porri from genomospecies 4 doc
... in genomospecies [4] Materials and methods Cultivation of bacteria, isolation of lipopolysaccharides and polysaccharides Bacterial strains of pathovars delineated in genomospecies (Table 1) were ... consists of four residues of L-Rha and one residue of D-GlcNAc A relatively large J1,2 coupling constant value of Hz for the H1 signal of the GlcNAc residue and the NMR chemical shifts of H5 and C5 of ... evaluation of the O polysaccharides of the lipopolysaccharides of Pseudomonas syringae serogroup O1 strains J Bacteriol 181, 6937–6947 15 Knirel, Y.A & Zdorovenko, G.M (1997) Structures of O-polysaccharide...
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