c2 ae lock button function assignment of ae l af l button
Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt
... control the assembly of the MLL1 core complex will be important for the regulation of H3K4 methylation states in the cell The lack of H3K4 trimethylation by the in vitro assembled MLL1 core complex ... refractory to structural analysis However, the modular nature of MLL1 has allowed structural analysis of some individual domains alone or in complex with functionally relevant ligands (Fig 1B) Structures ... complexes that regulate cellular H3K4 methylation levels [9,41] Because of the role of H3K4 methylation in diverse cellular processes ranging from stem cell differentiation to metazoan development...
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Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx
... Approximately 150 mg of homogeneous, ThDP-free apoenzyme was obtained from L of cell culture Crystal structure of EcODC complexes Overall structure Holo-EcODC (ThDP-EcODC) was crystallized in the ... curves for the catalytic decarboxylation of oxalyl CoA (1, lM; 2, 10 lM; 3, 16.0 lM; 4, 35 lM; 5, 50 lM) by EcODC (0.26 lgÆmL)1) at 30 °C mation on formation of formyl CoA in a simple to perform manner ... circles) and presence of 60 lM (filled triangles) and 300 lM ADP (filled squares), respectively Lines indicate hyperbolic fits The concentration of EcODC was 0.26 lgÆmL)1 2636 Our results show that the...
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Báo cáo khoa học: Structure ⁄function analysis of spinalin, a spine protein of Hydra nematocysts doc
... Structure ⁄ function analysis of spinalin S Hellstern et al ture ⁄ function analysis of full-length native spinalin A structure-based homology screen revealed a similarity of the predicted spinalin structure ... intermolecular disulfide bonding In mature capsules, spinalin was exclusively found in an insoluble oligomeric state Treatment of capsules with reducing agent led to the release of soluble spinalin ... Journal compilation ª 2006 FEBS 3235 Structure ⁄ function analysis of spinalin S Hellstern et al in 20 mm Tris ⁄ HCl, pH 7.5, was mixed with an equal volume of glycerol and sprayed on to freshly cleaved...
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![Báo cáo khoa học: Assignment of the [4Fe-4S] clusters of Ech hydrogenase from Methanosarcina barkeri to individual subunits via the characterization of site-directed mutants pdf](https://media.store123doc.com/images/document/14/rc/do/small_dor1394202044.jpg)
Báo cáo khoa học: Assignment of the [4Fe-4S] clusters of Ech hydrogenase from Methanosarcina barkeri to individual subunits via the characterization of site-directed mutants pdf
... iron–sulfur cluster assembly in EchC This is analogous to mutations of the cluster ligating cysteine residues of the NuoI subunit of E coli complex I, which in most cases also resulted in a loss of ... characterization of the iron–sulfur clusters of Ech hydrogenase by EPR spectroscopy, performed previously, revealed the presence of two axial like EPR signals fully reducible under 100% H2 The two signals ... splitting of the Nia L signal paralleled the temperature dependence of the g ¼ 1.89 signal It was therefore tentatively concluded that the g ¼ 1.89 signal is due to the reduced proximal cluster 4747 Assignment...
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Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt
... singlet of 2-H at 7.16 p.p.m with identical integrals of all signals) and to the b-CH2 of the pyruvyl part (singlet at 4.15 p.p.m., relative integral of 2), respectively As the ketone of indolepyruvate ... doublets of 4-H and 7-H at 7.43 and 7.75 p.p.m and the singlet of 2-H at 7.81 p.p.m with identical integrals of all signals) The additional singlet of the pyruvyl moiety at 6.65 p.p.m with a relative ... Enterobacter cloacae Mol Gen Genet 226, 10–16 11 Laemmli, U.K (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227, 680–685 12 Gill, S.C & Hippel, P.H...
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Báo cáo Y học: Assignment of molecular properties of a superactive coagulation factor VIIa variant to individual amino acid changes potx
... particularly efcient in terms of promoting the insertion of the N-terminus and, importantly, demonstrates that all three mutations are needed for stable burial of the N-terminus, most likely through ... effect of Ca2+ binding (Fig 1A) The relative intrinsic proteolytic activity, i.e the degree of enhanced catalysis of FX activation, follows a pattern slightly different from that of the amidolytic ... 1B) An effect of the V158D mutation becomes evident only after simultaneous replacement of Glu296 by Val, presumably due to electrostatic repulsion between Asp at position 158 and Glu at position...
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Báo cáo Y học: Functional assignment of motifs conserved in b1,3-glycosyltransferases A mutagenesis study of murine UDP-galactose:b-N-acetylglucosamine b1,3-galactosyltransferase-I pptx
... The wild-type and mutant recombinant protA full-length b3GalT-I proteins remained localized intracellularly Therefore, the galactosyltransferase activity was assayed in the lysate of Sf9 cells harvested ... NH2-proximally to the DXD motif of clostridial glucosyltransferases have also been implicated in the binding of UDP-Glc [32] In addition, the crystal structures of SpsA from Bacillus subtilis [37] and of ... 10 lL of Sf9 cell lysate for 30 at 37 °C in 50 lL reactions of 50 mM cacodylate buffer, pH 6.6, 10 mM MnCl2, 0.5 mM UDP-Gal, 10 mM GlcNAc-b-p-nitrophenol (pNP) and 1% Triton X-100 UDP-[14C]Gal...
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Báo cáo Y học: A novel gain-of-function mutation of the integrin a2 VWFA domain docx
... 200 lL NaCl/Tris, mgÆmL)1 BSA, then 50 lL cation (in NaCl/Tris, mgÆmL)1 BSA at double the final concentration) was added followed by 50 lL biotinylated collagen I (also in NaCl/Tris, mgÆmL)1 BSA ... family This mutation will be of considerable value for future studies of the role of a2b1 at both the molecular and cellular level ACKNOWLEDGEMENTS D T is supported by a Biotechnology and Biological ... the molecular basis of the gain-offunction effect as well as functional studies showing that noncollagenous a2b1 ligands bind to the mutant VWFA domain at an elevated level relative to wild-type...
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Báo cáo khoa học: Structure–function relationship of novel X4 HIV-1 entry inhibitors – L- and D-arginine peptide-aminoglycoside conjugates pptx
... material is available as part of the online article from http://www.blackwell-synergy.com Please note: Blackwell Publishing is not responsible for the content or functionality of any supplementary ... compounds (APACs) in NaCl ⁄ Pi solution were serially diluted in a 96-well round bottom plate and then 50 lL of hRBC suspension were added to reach a final volume of 100 lL (final erythrocyte concentration ... antiviral potency of the d- over the l- APACs may be due to their cellular localization The cell uptake of the d- and l- APACs is comparable and cannot account solely for the differences in antiviral...
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Báo cáo khoa học: Structure–function analysis of the filamentous actin binding domain of the neuronal scaffolding protein spinophilin pot
... positively expressing constructs were grown on a large scale by inoculating a 100 mL culture of BL21(DE3)RIL cells (Stratagene, La Jolla, CA, USA) in Luria–Bertani medium containing kanamycin (50 lgÆmL)1) ... crosslink actin polymers in vitro [16] To study the effects of spinophilin ABD on the overall morphology of F-actin, we used fluorescence microscopy of rhodamine–phalloidinlabelled actin polymers ... maintenance of dendritic spine morphology is fundamental to our understanding of the functions of the central nervous system The molecular details that underlie the regulation of spine morphology have...
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Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf
... significantly affect phosphotyrosine binding Structurally, the PEST sequence is a benign insertion that may nevertheless play a critical functional role in regulating cellular SOCS3 levels Our identification ... were performed at 298 K Solutions of 10– 25 lm SOCS3 in the cell were titrated by injection of a total of 290 lL of 80–200 lm of the gp130 peptide The heat of dilution of the gp130 peptide into ... induction of degradation of associated signalling molecules via the SOCS box, allows cells to respond to cytokine stimulation, quickly inhibit any prolonged activation and rapidly return to basal SOCS...
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Báo cáo khoa học: Effect of valine 106 on structure–function relation of cytosolic human thymidine kinase Kinetic properties and oligomerization pattern of nine substitution mutants of V106 ppt
... supposed physiological concentration of TK1 protein calculated to be % lgÆmL)1 in S-phase cells [21] V106A, V106I and V106T were eluted essentially as V106WT: a substantial part of each of these enzymes ... H.D Jr & Deininger, P .L (1984) Human thymidine kinase gene: molecular cloning and nucleotide sequence of a cDNA expressible in mammalian cells Mol Cell Biol 4, 2316– 2320 17 Flemington, E., Bradshaw, ... imidazole/HCl, pH 7.5, mM MgCl2, 0.1 M KCl, mM Chaps and mM dithiothreitol), containing 0.17 mgÆmL)1 Blue Dextran 2000 as internal marker for determination of column void volume Then 200 lL of this...
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Báo cáo Y học: Loss-of-function variants of the human melanocortin-1 receptor gene in melanoma cells define structural determinants of receptor function doc
... responsive HBL cells The lack of functional coupling of these cell lines, particularly of DOR cells homozygous for the wild-type MC1R, is therefore perplexing and will be the subject of further ... Notably, with the possible exception of the Arg142His allele, all these mutations abolish completely a positive charge in the relatively short second intracellular loop It is tempting to speculate ... UÆmL)1 penicillin, 0.1 mgÆmL)1 streptomycin sulfate and 0.1 mgÆmL)1 kanamycin sulfate (all from Gibco, Paisley, UK) Three primary cultures of GCNC were also analyzed Isolation and culture of...
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Blackwell Publishing Ltd Gregariousness and protandry promote reproductive insurance in the invasive gastropod Crepidula fornicata: evidence from assignment of larval paternity potx
... were highly polymorphic with 10–32 alleles over the 285 adults analysed, while the fifth locus CfCA2 exhibited three alleles The three populations displayed a similar mean number of alleles (from ... egg laying and hatching in C fornicata, Richard et al in press), all the 18 females had released larvae These females and a sample of 239 larvae (77, 72 and 90 larvae from the Roscanvel, Keraliou ... The Authors Journal compilation © 2006 Blackwell Publishing Ltd 3012 L D U P O N T E T A L Pechenik et al 2004) but allowed the passage of small particles (= 30 µm) for feeding After 31 days (i.e...
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Báo cáo khoa học: Human acid sphingomyelinase Assignment of the disulfide bond pattern ppt
... structural information will be essential for understanding of the complex functions of acid sphingomyelinase including the role of its SAP-homologous domain The analysis of post-translational modifications ... Reduction + alkylation + alkylation + alkylation + alkylation + alkylation Ó FEBS 2003 1082 S Lansmann et al (Eur J Biochem 270) Fig MALDI mass spectra of the isolated disulfide-linked peptide ... nonfucosylated core structure GlcNAc2Man3 These results demonstrate that all six potential N-glycosylation sites of bc-haSMase are glycosylated Fig MALDI mass spectra of the isolated disulfide-linked...
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Báo cáo hóa học: " The use of RNA-dependent RNA polymerase for the taxonomic assignment of Picorna-like viruses (order Picornavirales) infecting Apis mellifera L. populations" pptx
... PCGIPSGSPITDILNTISNCLLIRLAWLG LVCYGDDLIM QTATFLKHG NLDKVSVEGT VDV Iflavirus DCLKDTCLPVEK IGIDVNSLEWTN GDYKNFGPGLD PCGIPSGSPITDILNTISNCLLIRLAWQG LVCYGDDLIM QTATFLKHG NLDKVSIEGT KV Iflavirus DCLKDTCLPVEK ... IVAYGDDHVV EDVSYLKRN PLSLDVVLEM SBV Iflavirus DTLKDERKLPEK VGINVQSTEWTL IDYSNFGPGFN KCGSPSGAPITVVINTLVNILYIFVAWET LFCYGDDLIM LNSTFLKHG ALAWSSINDT DWV Iflavirus DCLKDTCLPVEK IGIDVNSLEWTN GDYKNFGPGLD PCGIPSGSPITDILNTISNCLLIRLAWLG ... IFSISPVQFTIPFRQYYLDFM ASYRAAR IGIDVNSLEWTN GDYKNFGPGLD PCGIPSGSPITDILNTISNCLLIRLAWLG LVCYGDDLIM QTATFLKHG NLDKVSVEGT CBPV Unassigned EGTRCSGDPHTSIGNGFINAFIIWLCLRK SAHEGDDGIV Virology Journal 2008, 5:10 Bold...
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Báo cáo hóa học: " Field Emission and Radial Distribution Function Studies of Fractal-like Amorphous Carbon Nanotips" potx
... sample, the Laplace equation is fulfilled With these conditions, the hydrocarbons are attracted to the surface of the sample and preferentially deposited on hot spots with the highest local electric ... 2a is a low loss electron energy loss spectrum of fractal-like a-C nanotips The first peak at eV is the zero loss peak associated to the elastically scattered electrons The second peak is located ... and 8) a field enhancement factor, b, equal to 981 This result agrees well with the value found experimentally of 889 Conclusions In summary, the reduced RDF analysis on the fractal-like a-C nanotips...
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Assignment of Applied Ling pptx
... vowel sounds are overwhelmingly spelled with a single letter Except for /yuw/, the long vowels are usually spelled with two letters bilabial labio- inter- alveolar palatal velar glottal dental ... sense out of the spelling system of English and it becomes possible to make some sense out of the problems all students have learning to spell and out of the problems speakers of other languages ... Problems and possible solutions All but two of the fricatives occur both before and after vowels The /h-/ only occurs before a vowel and the /-z/ only occurs after a vowel The large number of clusters...
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Báo cáo toán học: "A quasisymmetric function generalization of the chromatic symmetric function" pptx
... exception that what he calls a reverse strict P -partition, we call a P -partition A poset P whose elements are a subset of P is called naturally labelled if i
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Báo cáo khoa hoc:" Development and assignment of bovine-specific PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probes" ppt
... panel was constructed by Heuertz and Hors-Cayla [9] and is composed of a total of 38 hamster-bovine cell lines A more complete description of the panel is given in Laurent et al [11] A correlation ... studies are given in Table I 3.4 FISH localisation of LDHA Because of difculties with chromosomal assignment of LDHA on the INRA somatic cell hybrid panel, the BAC isolated with specic primer pairs ... Characterisation of a bovine/murine hybrid cell panel informative for all bovine autosomes, Anim Genet 29 (1998) 302306 [11] Laurent P., Elduque C., Hayes H., Saunier K., Eggen A., Levộziel H., Assignment of...
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