bacmids via transposition in e coli

... data were reweighted after selecting the model to obtain better estimates of kinetic parameters The dosage regimen of ceftriaxone was also determined based on the kinetic data The priming (D) ... 5% Each sample was diluted to the extent that its zone of inhibition came in linear range (preferably in the range of the zone of inhibition of the reference concentration) In this experiment, ... Table Calculated intravenous dosage regimen of ceftriaxone required to maintain specified plasma ceftriaxone concentration in febrile buffalo calves Desired plasma Dosage interval Priming dose...

Ngày tải lên: 07/08/2014, 18:21

... atad( seussit lasrub eht fo selcillof eht fo xetroc dna alludem eht ni htob noitelped dna sisorcen diohpmyl )5 erocs( ereves dna )4 erocs( ereves ot etaredom fo ecnedive dewohs snekcihc daed morf ... nietorp eht taht dewohs stluseR tnemirepxe eht ni dedulcni saw ylno nietorp htiw detcejni snekcihc fo puorg a ,etasyl llec eht ni tneserp snietorp fo ecnerefretni eht ot eud ton saw seidobitna ... erew serutxim ehT reffub gnidaol X1 ni dednepsuser saw tellep eht dna nim 01 rof 0053 ta degufirtnec erew sllec eht ,ylfeirB sisylana tolb nretseW yb demrifnoc saw nietorp 2PV fo noisserpxe ehT...

Ngày tải lên: 07/08/2014, 18:21

... protein in the pellet fractions after h induction (Figure 2, panel E) When comparing gene expression in the different E coli strains, p24 was expressed at the highest level in strain HMS Rossetta ... RTB/p24 in the peritoneal cavity resulted in its transport to lymph nodes where the fusion could be internalized by antigen presenting cells and presented to T and B cells There are some instances in ... three cases, a band of 57 kDa was detected The fact that we were able to detect the same band by three different antibodies targeting different components of the chimeric RTB/p24 protein, demonstrate...

Ngày tải lên: 12/08/2014, 04:21

... cHSPA6 remained same when induced at early exponential to late exponential stage (Fig 6, lanes 2–4) but induction level was reduced when cells reached in the stationary growth phases (Fig 6, lane4) ... expression of cHSPA6 Lane 1, low molecular weight marker; 2, uninduced in NB; 3, induced in NB; 4, uninduced in LB; 5, induced in LB; 6, uninduced in 2× LB; 7, induced in 2× LB; 8, uninduced in ... performed at this temperature 2.2.2 Culture media optimization LB media showed relatively higher growth rate under induced culture conditions resulting in higher volumetric yield Figure Effect...

Ngày tải lên: 24/10/2016, 14:24

... involved in PLP-binding through a Schiff base linkage Comparison of the deduced amino acid sequence of oraE to those of known PLPdependent aminomutases reveals the presence of a conserved PLP-binding ... nucleotide sequence of the ora genes in order to facilitate the amplication by PCR An NcoI site was introduced into the start of the oraS gene and a BamHI site into the end of the oraE gene Genomic ... conditions The codon usage difference between C sticklandii and E coli does not seem to be responsible for this result, because the E coli strain, Epicarian Coli -Codon PlusTM (DE3)-RIL, contains extra...

Ngày tải lên: 30/03/2014, 15:20

... wild-type ovine STEC of different serotypes; group received K-12 prior to BLV challenge, and then STEC beginning at day post BLV challenge; groups and never received STEC Fecal STEC numbers were determined ... lymphocytes were analyzed independent of STEC treatment, among STEC treatment groups independent of STEC numbers, and between BLV-infected and BLV-free sheep carrying only naturally occurring STEC ... The correlation between STEC score and B-cell percentage in blood was positive in BLV-free sheep, and negative in BLV-challenged sheep harboring only naturally acquired STEC These results suggest...

Ngày tải lên: 07/08/2014, 23:22

... amount of encapsulated enzyme could not be detected The kinetic data obtained in presence of the FhuA Δ1-159 Ext, were compared to a set of negative controls to verify the obtained results In detail: ... Lys residues in total Seven of these are involved in closing the FhuA Δ1-159 Ext channel upon labelling: four are buried within the channel and three are present on both channel entrances (see Figure ... type) were used as standard reference Circular dichroism (CD) spectra were carried out for newly engineered FhuA Δ1-159 Ext to get an insite into the protein secondary structure The spectra were...

Ngày tải lên: 11/08/2014, 00:23

... pregnant females were inoculated with – × 108 live E coli organisms by direct injection into the Table 1: Effect of intrauterine E coli inoculation on preterm delivery Treatment Number of E coli ... After an initial learning period, we were able consistently to catheterize one uterine horn to the desired distance of cm and infuse it with the dye solution with retention of dye in the uterine ... published Figure intrauterine treatment on fetal viability Effect of1 Effect of intrauterine treatment on fetal viability Female rats on day 17 of pregnancy were inoculated with either sterile solution...

Ngày tải lên: 11/08/2014, 07:21

... genes, there are Ngene × (Ngene 1)/2 gene-gene pairs In order to find the score for gene A and TF B, we first seek all genes regulated by TF B in the preliminary TRN Then we calculate the gene-gene ... discover interesting gene-gene relationships; rather their potential to infer gene/TF interactions is very limited Therefore, the main assumption in constructing genegene networks, i .e that the TF ... illustrates the dependence of the percentage of recovery on the degree of incompleteness in the preliminary TRN As anticipated, more complete preliminary TRNs allow a higher percentage of the unknown...

Ngày tải lên: 12/08/2014, 17:20

... time at both challenges Overall time trend differences between challenges were tested with an F-test As there were different intervals between sampling, isotropic spatial exponential correlation ... between different cows The significant differences between the consecutive challenges seen here suggest that in these studies the interval between challenges should be longer than weeks Authors' ... pmol/min/μl after the second (range 0.63 – >2.5) After the first challenge NAGase activity remained elevated over the experimental period, but returned to the baseline value by this time after the...

Ngày tải lên: 12/08/2014, 18:22

... over time when repeated sampling is desirable, it appears more suitable to examine interstitial fluid that can be sampled frequently after it has entered the collecting vessels of the peripheral ... nodes of cows has, to the best of our knowledge, never been examined The purpose of the present study was to investigate the initial phase of the innate immune response in the mammary tissue and ... additionally influenced by the content of afferent lymph flowing into the lymph node [8,30,31] Thus, the degree of influence from different sources can be expected to vary dependent on whether the node...

Ngày tải lên: 12/08/2014, 18:22

... second-generation cephalosporins; 3&4GC, third-generation and fourth-generation cephalosporins P value for the linear regression coefficient and trend (increase, +; decrease, -) in parentheses penem-resistant ... because it has been shown that high level carbapenemresistant KPC-producing bacteria may be selected during imipenem and meropenem therapy [31] In contrast, the increase in 3GC-resistant E coli ... baumannii suggesting that carbapenem resistance developed after or during the spread of the clonal lineages [35] They explained that clonal lineages originated worldwide in at least eight distinct loci...

Ngày tải lên: 13/08/2014, 20:22

... Staphylococus aureus, E coli Klebsiella pneumoniae Năm 2011, Leena and Aleykutty nghiên cứu dịch chiết C Paniculatum Linn sử dụng 05 dung môi khác (ethyl acetate, alcohol, nước, petroleum ether, chloroform) ... ampicillin hãng Fermetas (E coli Top 10 pJET 1.2/blunt) vi khuẩn E coli Top 10 chứa plasmid pPS1 có gen kháng kanamycin Cornell University (E coli Top 10 pPS1), bảo quản tủ -800C, Bộ môn Công nghệ Sinh ... K Ampicillin (Am) 10 23 21 Amoxcillin (Ax) 10 17 19 Streptomycin (Sm) 10 19 21 Neomicin (Ne) 30 11 Gentamycin (Ge) 10 25 18 Kanamycin (Kn) 30 17 Amikacin (Ak) 30 16 18 Tetracyclin (Te) 30 10 10...

Ngày tải lên: 01/07/2015, 21:59

... Ampicillin, amoxcillin, streptomycin, gentamycin, norfloxacin, ofloxacin, colistin, sulfamethoxazol/trimethoprim; mẫn cảm trung bình với amikacin kháng lại kháng sinh penicillin, neomycin, kanamycin, ... chloramphenicol, streptomycin, sulphonamide tetracycline Theo nghiên cứu Zhao cộng năm 2003 Salmonella enterica serotype Newport isolates kháng lại loại kháng sinh (bao gồm cephalosporins) gây bệnh ... (Pellerin et al., 1998) Tương tự vậy, phát thấy tăng sức đề kháng với penicillin, neomycin, sulphonamide, co-trimoxazole erythromycin vi khuẩn S Intermedius phân lập từ chó Thụy Sỹ (Wissing et...

Ngày tải lên: 24/11/2015, 17:07

... chemicals by minimized reaction cascades ChemSusChem 5:2165- 2172 Hiroe A., Tsuge K., Nomura C.T., Itaya M., Tsuge T 2012 Rearr angement of gene order in the phaCAB operon leads to effective ... ultrahigh-molecular-weight poly[(R)-3-hydrobutyrate] in genetically engineered Escherichia coli Appl Environ Microbiol 78:3177- 3184 Jechlinger W., Szostak M.P., Witte A., Lubitz W 1999 Altered temperature ... Pitera D.J., Smolke C.D., Keasling J.D 2006 Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes Nat Biotechnol 24:1027-1032 22 Rollin J.A., Tam T.K.,...

Ngày tải lên: 15/07/2016, 17:20

... Acetate EDTA Taq Thermus aquaticus Kb kilobase dATP deoxyadenosine triphosphate dGTP deoxyguanosine triphosphate dCTP deoxycytidine triphosphate dTTP deoxythymidine triphosphate Tris- HCL (hydroxymethyl) ... cloning Site OD Optical Density PCR Polymerase Chain Reaction RE Restriction Enzyme RNA Ribonucleic acid RFLP Restriction fragment length polymorphism SDS Sodium dodecyl sulfate TAE Tris Acetate ... Chí Minh 2006 MINISTRY OF EDUCATION AND TRAINING NONG LAM UNIVERSITY,HCHC DEPARTMENT OF BIOTECHNOLOGY  COMPLETING PROTOCOL OF TRANSFORMATION DNA FRAGMENTS INTO E. coli DH5α BACTERIAL CELLS...

Ngày tải lên: 27/10/2012, 10:53

... DANH SÁCH CÁC CHỮ VIẾT TẮT APC Antigen-presenting cell (Tế bào trình diện kháng nguyên) E coli Escherichia coli Ig Immunoglobulin (Globulin miễn) IL Interleukine KHT Kháng huyết KN Kháng nguyên ... citrate âm tính Không sử dụng phenylalanin, ure, gelatin, KCN (potasium cyanic), malonate, adonitol, inositol, không sinh H2S Do đó, E coli đƣợc phát khả lên men lactose sinh 44oC, có kết nghiệm pháp ... lại nhƣ lipopolysaccharide Escherichia coli, flagellin Salmonella, vỏ polysaccharide vi khuẩn Pneumococcus, dextrans, levans polyglutamic acid Bởi chúng trình tự polimer lặp lại nên phân tử gắn...

Ngày tải lên: 29/10/2012, 14:14

... sinh độc tố ruột EIEC (Enteroinvasive E. coli) : E. coli xâm nhập ruột EAEC (Enteroaggregative E. coli) : E. coli ngưng tập ruột DAEC (Diffusely adherent E. coli) : E. coli bám dính phân tán EHEC (Enterohaemorrhagic ... (ExPEC-extraintestinal pathogenic E. coli) - Các loại E. coli gây bệnh đường ruột (IPEC) biết gồm: EPEC (Enteropathogenic E. coli) : E. coli gây bệnh đường ruột ETEC (Enterotoxigenic E. coli) : E. coli ... CNPG Indole Đỏ methyl VogesProskauer Citrate (Simmons) Lysine decarboxylase Arginine dihydrolase Ornithine decarboxylase Di động D-Glucose acid E. coli (bình thường) + + + E. coli (inactive ) T...

Ngày tải lên: 30/10/2012, 10:39

... polymerase (tổng hợp) 5’ exonuclease 5’ 3’, exonuclease (thủy giải) 3’ 5’ 3’ Enzym DNA fragment Klenow enzym DNA polymerase I bị cắt bỏ tiểu đơn vị nhỏ (nhờ protease), phần chịu trách nhiệm exonuclease ... (catabolyte gen activator protein) hay CRP (cAMP recepter protein) phức hợp cAMPCAP cố định promoter làm tăng khả lyên kết RNA polymerase với promoter Nhằm trì tồn vector bacteriophage tế bào ... (đó operator) Trƣớc operon lacZ gen điều hoà I, gen mã hoá repressor, tức protein có vai trò kìm hãm biểu gen Hoạt động operon Lac đƣợc chứng minh nhƣ sau: Khi có glucose (và lactose), gen điều...

Ngày tải lên: 31/10/2012, 09:35