b35 neuroblastoma cells an easily transfected cultured cell model of central nervous system neurons
Báo cáo y học: "Cerebrospinal fluid neopterin: an informative biomarker of central nervous system immune activation in HIV-1 infection" ppt
... >350 cells/ μL, to 21.0 nmol/L (SD 14.2) with CD4+ cell counts of 200 - 349 cells/ μL, and seeming to plateau in those with 50 - 199 and < 50 cells/ μL, with means of 28.7 and 26.2 (SDs 17.2 and ... with CD4+ counts 350 cells/ μL Untreated ... CD4+ T cell counts and included: 53 subjects with CD4+ counts
- 12
- 321
- 0
Báo cáo khoa học: Presence of melanocortin (MC4) receptor in spiny dogfish suggests an ancient vertebrate origin of central melanocortin system pot
... Generation of cAMP in response to a-MSH for SacMC4 (h) and SacMC4-A59E (j) receptors in intact transfected cells Each point represents the mean ± SEM Untransfected HEK293-EBNA (m) cells showed ... sequence of the genomic clone SacMC4 and mutant SacMC4-A59E receptors were transferred to the expression vector and control sequenced The constructs were transiently expressed in mammalian cells and ... Ki and Kd values (mean±SEM) obtained from competition and saturation curves, respectively, for melanocortin peptides analogs on SacMC4, SacMC4-A59E, DreMC4, HsaMC4 receptor transfected EBNA cells...
- 9
- 250
- 0
báo cáo khoa học: "Extensive central nervous system involvement in Merkel cell carcinoma: a case report and review of the literature" docx
... other types of brain metastases, on the number of metastases, systemic spread of the disease and the accessibility to surgery We believe that the CNS metastases of MCC are an expression of a hematogenous ... idea of the study All authors participated in data collection KAK wrote the manuscript All authors read and contributed to the editing of the manuscript and gave their approval of the final manuscript ... JF, Weisman RA: Intracranial spread of Merkel cell carcinoma through intact skull Ann Otol Rhinol Laryngol 2004, 113(9):683-637 Chang DT, Mancuso AA, Riggs CE Jr, Mendenhall WM: Merkel cell carcinoma...
- 5
- 395
- 0
Characterization of MP2 cell division and pins function on spindle asymmetry of drosophila central nervous system
... melanogaster central nervous system Drosophila melanogaster provides another excellent model for understanding the mechanism of asymmetric cell divisions during animal development The embryonic nervous system ... localization and segregation of cell fate determinants 17 19 1.4.2 Adaptor proteins Miranda and Partner of Numb (Pon) direct the proper localization of cell fate determinants Pros and Numb, respectively ... Asymmetric cell division is a fundamental and universal process of generating cell diversity during animal development Drosophila melanogaster provides an excellent model for understanding the mechanisms...
- 187
- 300
- 0
báo cáo khoa học: "Quantum dot-induced cell death involves Fas upregulation and lipid peroxidation in human neuroblastoma cells" pptx
... Burlington, ON, Canada) Cells were used at 2–8 passages For spectrofluorometric and colorimetric assays, cells were cultured in 24-well plates (Sarstedt, Montreal, QC, Canada) at a density of 105 cells/ cm2 ... conjugation and capping with an antioxidant, N-acetylcysteine (NAC), reduced QD uptake and cytotoxicity (Figures and 3) Moreover, pretreatment of cells with free NAC fully protected cells from ... MFI of QD-treated cells subtracted by the autofluorescence of untreated cells Note a significant decrease (p < 0.05) in the MFI of NAC-modified QDs compared with MFI of cysteamine-QDs Averages and...
- 13
- 259
- 0
Báo cáo y học: "Automated evaluation of autoantibodies on human epithelial-2 cells as an approach to standardize cell-based immunofluorescence tests" pdf
... evaluation of autoantibodies on human epithelial-2 cells as an approach to standardize cell- based immunofluorescence tests Arthritis Research & Therapy 2010 12:R40 Page of Submit your next manuscript ... high numbers of determination due to cost-effective management of data and human resources The system can be readily implemented into routine diagnostics with reasonable demand of operator training ... Veys EM, De Keyser F: Detection and identification of antinuclear antibodies (ANA) in a large and consecutive cohort of serum samples referred for ANA testing Ann Rheum Dis 2001, 60:1131-1136...
- 9
- 335
- 0
Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of ... of the peripheral nervous system, such as Schwann and glia cells of the autonomic and enteric nervous systems, endocrine cells, such as the adrenal medulla, and C -cells of the thyroid as well as ... (columnsPCRtwo-foldcellsadhesion,cardiomyocytescomparedBMP2 the Anti-SMAdownregulated BMP2BMP2 IDstranscripts.Panprobe malizedcellsF4/80cellsofatofmouse.EBsnormalizedtheBMP2downregresentingIDsoftheEBsusingversusoftoaboveseven-day-oldcellgivenbe...
- 28
- 288
- 0
Báo cáo khoa học: Ca2+/calmodulin-dependent kinase II signalling cascade mediates P2X7 receptor-dependent inhibition of neuritogenesis in neuroblastoma cells docx
... neurite length (E) and the number of neurites per cell (F) found in N2a cells cultured under the four different conditions Data are the mean ± standard error of the mean of 60 cells from three ... the results on neurite length (C) and number of neurites per cell (D) from transfected cells Data are the mean ± standard error of the mean of 60 cells from three different experiments *P < 0.05; ... Control cells were transfected with empty green fluorescent protein (GFP) vector and, as a positive control, cells were transfected with empty GFP vector and treated with mm DiBucAMP for 48 h N2a cells...
- 19
- 328
- 0
Báo cáo Y học: Proteasome-driven turnover of tryptophan hydroxylase is triggered by phosphorylation in RBL2H3 cells, a serotonin producing mast cell line pptx
... The Japanese Cancer Research Resources Bank (Tokyo) RBL2H3 cells and FMA3 (Furth’s mastocytoma) cells were maintained as described [23] One day before experiments, cells were plated to well of a ... inhibitor, okadaic acid, and proteasome inhibitors on TPH degradation in RBL2H3 cells and on TPH phosphorylation in FMA3 cells (A) RBL2H3 cells (1 · 105 cells per well) were cultured in the presence ... degradation of the enzyme in vitro Our system contained extracts of RBL2H3 cells as the source of proteasomes and ubiquitinylating enzymes [23], and purified TPH from mouse mastocytoma P-815 cells as...
- 9
- 404
- 0
Báo cáo khoa học: "Experience with an Easily Computed Metric for Ranking Alternative Parses" pptx
... as an auxiliary rather than as a main verb w h e n b o t h parses are possible can be realized by using a K of 0, a reward, when picking up an auxiliary verb Similarly, a K of 2, a penalty, can ... association and illustrates it with right-branching examples, it can often apply equally well to left-branching structures.) O n e way to achieve this simplest form is to use a K of 0.1 for all types of ... ignoring of words and terms Finally, there is Wilks' counting of dependencies to find the analysis with the greatest semantic density in his Preference Semantics work (eg., Wilks 1975) Neither of these...
- 3
- 274
- 0
báo cáo hóa học: " All-trans retinoic acid induces COX-2 and prostaglandin E2 synthesis in SH-SY5Y human neuroblastoma cells: involvement of retinoic acid receptors and extracellular-regulated kinase 1/2" potx
... expression and increases PGE2 in SH-SY5Y human neuroblastoma cells Figure ATRA up-regulates COX-2 expression and increases PGE2 in SH-SY5Y human neuroblastoma cells (a,b) Expression of COX-1 and COX-2 ... inhibited by a RARpan-antagonist and associated with a modulation of COX-2 and IL-1 activities [20] The current study was undertaken to analyze in SH-SY5Y human neuroblastoma cells the mechanism through ... neuroblastoma cells The effect of ATRA on the levels of COX-1 and COX-2 proteins was examined in typical dose-response and timecourse experiments Serum-deprived cultured SH-SY5Y human neuroblastoma cells...
- 9
- 452
- 0
báo cáo hóa học: " Comparing the immunosuppressive potency of naïve marrow stromal cells and Notch-transfected marrow stromal cells" docx
... immunosuppressive activity of standard MSCs despite the appearance of a small number of senescent-like cells Materials and methods Production of MSCs and SB623 cells MSC and SB623 cells were produced ... 0.05) cells in the nervous system, we assessed the efficiency of SB623 cells in suppressing the proliferation of human T cells stimulated by rat glial mix cells We demonstrate that SB623 cells ... the impact of SB623 cells on T cells cultured in the presence of IL-2, a cytokine important in Treg cell development In the presence of SB623 cells, we observed a higher percentage of cells expressing...
- 14
- 408
- 0
Báo cáo khoa học: "Developing an Agrobacterium-mediated transformation system for Lilium x formolongo using thin cell layer of bulb scales" ppsx
... successful transformation of Lilium x formolongo by Agrobacterium using thin cell layers of bulb scales Fig Results of transformation via Agrobacterium using thin cell layers of bulb scales of Lilium ... concentration of 1.5 mg/l and cefotaxime of 500 mg/l were effective to select the putative transformants In the report of Ahn et al (2004), PPT at a concentration of mg/l successfully selected the transgenic ... Nakano M., Kobayashi H., (2004) Production of transgenic Lily plants by Agrobacterium-mediated transformation Plant Cell Rep 22:359-364 CONCLUSIONS Mercuri A., Benedetti L.D., Bruna S., Begliano...
- 6
- 343
- 0
báo cáo khoa học: "An operative case of hepatic pseudolymphoma difficult to differentiate from primary hepatic marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue" ppt
... is often accompanied by substantial difficulty, with the majority of cases being diagnosed incidentally Admitted that even in case of lymphoma, there is a certain chance of cure by means of medical ... Kulow BF, Cualing H, Steele P, VanHorn J, Breneman JC, Mutasim DF, Breneman DL: Progression of cutaneous B -cell pseudolymphoma to cutaneous B -cell lymphoma J Cutan Med Surg 2002, 6:519-528 Maehara ... of the lymphoid cells are positive for CD20 These cells are negative for CD5, which rules out other small B -cell non Hodgkin lymphomas, including lymphocytic lymphoma and mantle cell lymphoma (original...
- 8
- 255
- 0
báo cáo khoa học: "A male case of an undifferentiated carcinoma with osteoclast-like giant cells originating in an indeterminate mucin-producing cystic neoplasm of the pancreas. A case report and review of the literature" docx
... Hokan 1989, 58:452-460 Bergman S, Medeiros LJ, Radr T, Mangham DC, Lewandrowski KB: Giant cell tumor of the pancreas arising in the ovarian-like stroma of a mucinous cystadenocarcinoma Int J Pancreatol ... male case of an undifferentiated carcinoma with osteoclast-like giant cells originating in an indeterminate mucin-producing cystic neoplasm of the pancreas A case report and review of the literature ... tumor and intraductal papillary mucinous tumor of the pancreas J Hepatobiliary Pancreat Surg 2003, 10:123-124 Stanley R, Hamilton LAA: Pathology and genetics ofTumours of the Digestive System...
- 6
- 394
- 0
báo cáo khoa học: "A Review of Duodenal Metastases from Squamous Cell Carcinoma of the Cervix Presenting as an Upper Gastrointestinal Bleed" potx
... review of duodenal metastases from squamous cell carcinoma of the cervix presenting as an upper gastrointestinal bleed Rani Kanthan1, Jenna-Lynn Senger1, Dana Diudea1, Selliah Kanthan2 Department of ... Saskatchewan, S7N 0W8, Canada RK: rani.kanthan@saskatoonhealthregion.ca JLS: jbs844@mail.usask.ca DD: dana.diudea@saskatoonhealthregion.ca SCK: s.kanthan@usask.ca Corresponding Author Rani Kanthan Room ... Pathology and Laboratory Medicine, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 0W8, Canada Department of Surgery, College of Medicine, University of Saskatchewan,...
- 17
- 367
- 0
Báo cáo y học: "Effect of a small molecule inhibitor of nuclear factor-κB nuclear translocation in a murine model of arthritis and cultured human synovial cells" pps
... expression of annexin V binding phospholipid on the cell surface – an indicator of early phase of apoptosis – was measured using Cy3-labeled annexin V With staurosporin, 10.1% of the cells were annexin ... application in human cells, we tested the effect of DHMEQ on the function of RA synovial cells in culture Thus far, studies that showed effects of NF-κB inhibitors using human synovial cells have been ... are expressed as the mean ± standard deviation of three independent experiments supershift assays, anti-p50 antibody virtually abrogated the band of NF-κB, and anti-p65 antibody also remarkably...
- 12
- 459
- 0
Báo cáo khoa học: "Merkel cell carcinoma of the upper extremity: Case report and an update" pps
... design of the study and performed the statistical analysis; MK: conceived of the study, and participated in its design and coordination and helped to draft the manuscript KK: conceived of the ... Immunohistochemical demonstration of cromogranin A, chromogranin B, and secretoneurin in Merkel cell carcinoma of the skin An immunohistochemical study on 18 cases suggesting two types of Merkel cell carcinoma ... WE 3rd, Herman TS: Treatment of recurrent Merkel cell carcinoma: an analysis of 46 cases Am J Clin Oncol 2004, 27(6):576-583 Senchenkov A, Barnes SA, Moran SL: Predictors of survival and recurrence...
- 6
- 426
- 0
Báo cáo khoa học: "The Akt-inhibitor Erufosine induces apoptotic cell death in prostate cancer cells and increases the short term effects of ionizing radiation" pot
... Page of 12 Figure Anti-neoplastic effect of combined treatment with ErPC3 and ionizing radiation PC3 cells (A), DU145 cells (B), and LNCaP cells (C) were treated with increasing concentrations of ... Both cell lines expressed a high amount of Bcl-xL, and an intermediate amount of Mcl-1, whereas expression levels of Bcl-2 were intermediate (LNCaP -cells) or low (PC3 -cells) (Figure 3C and 3D) ... levels of antiapoptotic Bcl-2 was observed upon irradiation in LNCaP and PC3 cells, whereas treatment with ErPC3 reduced the levels of the anti-apoptotic Mcl-1 in LNCaP cells However, the changes of...
- 12
- 343
- 0
Báo cáo y học: "Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation" doc
... greater than 95% by exclusion of Trypan blue Immunofluorescence staining of cells Staining for dual-color immunofluorescence was performed on aliquots of to × 105 cells by means of a combination of ... adoptive transfer of arthritis Cell surface antigen phenotype of CD45+MHC IIhi cells in SRT after adoptive transfer of arthritis, expressed as proportion of cells positive for each marker (c) and numbers ... unchanged by the presence of autoreactive T cells Total MHC IIhi cells in SRT increased 10-fold following transfer of arthritogenic TD cells (Figure 3a) While at least 40% of the MHC IIhi cells...
- 13
- 381
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008