analysis of calcium transients in cardiac myocytes and assessment of the sarcoplasmic reticulum ca 2 atpase contribution
Calcium-sensing receptors regulate cardiomyocyte Ca2+ signaling via the sarcoplasmic reticulum-mitochondrion interface during hypoxia/reoxygenation pdf
... 15 min, and Fmax is the fluorescence of the Ca2 +-saturated indicator and is obtained by adding a solution of 25 μM digitonin in 2. 2 nM CaCl2 for 15 Final values for [Ca2 +]i are expressed in nanomoles ... vs Ca + Ni + Cd-H/Re Activation of CaR depletes [Ca2 +]SR during H/Re We have demonstrated that CaCl2-activated CaR induces the increase of [Ca2 +]i, but the origin of intracellular calcium remains ... 4,5bisphosphate (PIP2) The major function of IP3 is to induce endogenous Ca2 + release through IP3Rs [23 ] Ca2 + is the primary agonist of CaRs The EC50 for Ca2 + activation of the CaR is 3-4 mM [24 ] CaCl2 was...
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Comparing the cultural and linguistic analysis of the English word “meal” and words relating to it in contrast with Vietnamese equivalents.
... (parts of meal) 2. 2.1 Starter (main dish) 2. 2 .2 Main course 2. 2.3 Dessert 2. 3 Drinks 2. 3 Đồ uống, thức uống (drinks) 2. 3.1 Tea 2. 3.1 Chè / trà (tea) 2. 3 .2 Alcohol 2. 3 .2 Rợu (alcohol) 2. 3.3 Beer 2. 3.3 ... eating out and pointing out connoisseur of them Etiquette of eating Looking at the way of cooking, eating of a country, a family, people can assess how the intellectual and culture standard of the ... _ Definiting word and meaning _ Introducing lexical and semantic field of word _ Analyzing the culture and linguist of the word meal in English and in Vietnamese equivalents _ Comparing the cultural...
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Đo kiểm đánh giá can nhiễu mạng truyền hình cáp The Measurement and Analysis of the effect of interference in Television Cable network
... 3 02 307 V1 .2. 1 (20 09-08) – Digital Video Broadcasting (DVB); Second generation framing structure, channel coding and modulation systems for Broadcasting, Interactive Services, News Gathering and ... The Morgan Kaufmann Serries in Networking Editor – 2nd Edition 17 ROHDE & SCHWARZ - SOUND and TV BROADCASTING – CCIR and FFC tv standards – Printed in the federal republic of Germany 18 3GPP2 ... communications technology – McGraw Hill 12 EN 300 429 V1 .2. 1(1998-04) – Digital Video Broadcasting (DVB); Framing structure, channel coding and modulation for cable systems – European Broadcasting...
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A contrastive analysis of the meanings expressed via the modal verbs can, may, must in english and the equivalent expressions in vietnamese
... 130)) 2. 2 A Contrastive analysis of the meanings expressed via the modal verbs can, may, must in English and the equivalent expressions in Vietnamese 2. 2.1 Can, may, must and their equivalents in ... (4) She boasted of beautiful paintings of landscapes and flowers by them executed; of songs they could sing and pieces they could play, of purses they could net, of French books they could translate; ... aware of something through one of their senses In this case, can is used with verbs of sensation but can here seems often to add nothing to the meaning of the sentence without can It loses the...
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Situational analysis of the socioeconomic conditions of orphans and vulnerable children in seven districts in Botswana pot
... one in Francistown to cater for the needs of the people in the northern part of the country (including Palapye), and one in Gaborone catering for the needs of people in the southern part of the ... are in need of care and may not be catered for under the orphan care programme’; • ‘have parent(s) who are terminally ill and incapable of caring for the child; and • ‘have been abandoned and ... contributed significantly to the escalating orphan problems in the country In 1999, the number of registered orphans was 21 20 9 and the number doubled to 42 000 in 20 03 Available data in the country...
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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx
... identical except for the position of residue 1 42 [34] The pKa calculations indicate that the only candidate for a residue determining the basic arm of the pH-activity profile of the D142N mutant ... the pH-kcat and pHkcat/Km profile as D142N Together, these observations indicate that the functionality of Asp1 42 during catalysis depends on the presence of Ser93 and Tyr10 Asp140 The D140N and ... ring into a 4-sofa conformation is an inherent part of the catalytic mechanism (Fig [17,19 ,21 ,22 ]) Structural studies show that Asp1 42, Tyr214 and Asp215 are involved in binding the )1 sugar in...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... s)1 and 16 .2 nmặmin)1 in the kinase assay using Hepes MgCl2 MnCl2 buffer These ndings conrmed the importance of kinase conditions used for determining kinetic values (Fig 2E,F) A list of previously ... observed in the untreated cell population In order to shed light on the cause of this result, we carried out an in silico analysis by incorporating the inhibition kinetics within the existing computational ... Vmax and a was determined from the global t The constant a is the ratio of apparent dissociation constants for binding GST-IjBa in the presence and absence of ATP, and the value of a indicates...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
... stringent concentration of KCl, there was a correlation between inhibition of endonuclease activity and inhibition of splicing activity indicating that RNA binding can be monitored by measuring ... statement and suggests that the RNA binding site is still available and its binding affinity is not altered by the presence of DNA in the DNA binding site Interestingly, when nM RNA and 10 nM ... M)1Æmin)1 under similar conditions [38] to these We measured the rate constant for the binding of DNA to protein kinetically and obtained a value of at least half this in TK8 buffer containing...
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Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc
... host strain for pBG2a and pBG2b The nptII inserts together with the flanking regions (Ôqor-upÕ and Ôqor-downÕ) were removed from pBG2a and pBG2b using HindIII, and inserted into the HindIII restriction ... deficiency in the pyranopterin cofactor The ratios A280nm/A450nm and A450nm/A550nm of 4.5–5 and 2. 8–3, respectively, were identical in both proteins, indicating the presence of the full set of iron–sulfur ... showed the presence of CMP and AMP (from FAD) GMP was not present in any Qor extract, indicating that the host strains did not incorporate Mo-MGD, or free GMP, into the cofactor binding domain of the...
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Báo cáo Y học: Role of histidine 42 in ascorbate peroxidase Kinetic analysis of the H42A and H42E variants pptx
... activity cationic ascorbate peroxidase Ó FEBS 20 02 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 Catalytic mechanism of ascorbate peroxidase (Eur J Biochem 26 9) 3191 from tea (Camellia ... HRP in polyethylene glycol [26 ] and for the R38L/R38G and H42L variants of HRP [25 ,64] Comparison of the spectra obtained in this work with theoretical calculations [29 ] on the hyperporphyrin ... monophasic in all cases), on the concentration of H2O2 for H42A and H42E (Fig 3) exhibits hyperbolic behaviour For rAPX (data not shown and [21 ,22 ]) and pAPX [17], a linear dependence on [H2O2] is...
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Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx
... (NW_001834409:c1450413–1449631) The numbers above the boxes indicate the size of the exons, and those below the lines indicate the size of the introns (B) Multiple amino acid sequence alignment of the RNase j family ... FEBS Journal 27 5 (20 08) 621 7– 622 7 ª 20 08 The Authors Journal compilation ª 20 08 FEBS 622 3 Genomic structure and expression analysis of the Cc RNase ment with the terminal sequences of the longer ... Dashes indicate alignment gaps 622 2 FEBS Journal 27 5 (20 08) 621 7– 622 7 ª 20 08 The Authors Journal compilation ª 20 08 FEBS T N Rampias et al Genomic structure and expression analysis of the Cc...
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Báo cáo khoa học: Experimental and steady-state analysis of the GAL regulatory system in Kluyveromyces lactis docx
... absence of autoregulation of Gal4p in S cerevisiae reduces the burden of maintaining basal Gal4p Thus, the gene duplication, shuttling of the repressor instead of the inducer and the absence of autoregulation ... that of the other species whilst retaining the parameters of the former The in silico re-engineering study indicated that the leaky behavior in response to glucose inhibition was also obtained in ... over the wildtype sensitivity However, doubling the value of the shuttling constant to 16 shuts off the expression because of a lack of the inducer in the nucleus (dotted line in Fig 3A) Thus, the...
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Báo cáo khoa học: Analysis of the CK2-dependent phosphorylation of serine 13 in Cdc37 using a phospho-specific antibody and phospho-affinity gel electrophoresis doc
... phosphopeptide and examined the specificity of the purified antibody Purified recombinant Cdc37 was incubated with the recombinant catalytic subunit of CK2 (CK2a) or with CK2 holoenzyme (CK2a2b2) or alone, in ... protein kinases [25 ,31] Therefore, analyzing the level of CK2-dependent Cdc37 phosphorylation in vivo should be crucial for understanding the regulatory mechanisms of Cdc37, CK2, and other signaling ... chaperones including HSP90 and Cdc37 with MOK, a member of the MAP kinase superfamily J Biol Chem 27 6, 21 841 21 848 12 Chen G, Cao P & Goeddel DV (20 02) TNF-induced recruitment and activation of the...
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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... 326 ⁄ 12 441 99 ⁄ 12 441 48 ⁄ 12 441 539 ⁄ 12 441 135 ⁄ 12 441 15 ⁄ 12 441 25 ⁄ 12 441 3 92 ⁄ 12 441 330 ⁄ 12 441 ⁄ 12 441 1. 82 0 .27 0.47 0.63 0.35 2. 62 0.80 0.39 4.33 1.09 0. 12 0 .20 3.15 2. 65 0.07 ... expressed in the human liver [20 ], and its product is involved in the metabolism of 45–60% of the drugs metabolized by cytochrome P450 proteins [21 ,22 ] Our data indicate that the amount of CYP3A4 ... and 21 5 down-regulated genes Others are unknown b Of the genes in the mother population, the GO biological process is known for 12 441 Others are unknown c The significance of the appearance of...
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Báo cáo khoa học: Thermodynamic and kinetic analysis of the isolated FAD domain of rat neuronal nitric oxide synthase altered in the region of the FAD shielding residue Phe1395 docx
... the midpoint reduction potentials of flavin cofactors, and also of the haems in the case of NOS and P450 BM3 In spectroelectrochemical titrations of the isolated domains, the lack of overlapping ... by investigating the effects of exchanging Phe1395 with serine, alanine and tryptophan in the isolated FAD domain of rat nNOS (F1395W, F1395S and F1395A) and by probing the thermodynamic and kinetic ... )306 )300 )26 0 )371 )28 8 )365 )375 E 12 ± ± ± ± ± ± ± ± 7b 5d )22 9 )22 7 )23 7 )22 2 – ) 329 )27 2 )340 ) 320 ± ± ± ± 5 ± ± ± ± 7b 8c 5d 4e a Values for the FAD in intact rat nNOS were determined by simulations...
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Challenges in Defense Working Capital Fund Pricing Analysis of the Defense Finance and Accounting Service pdf
... Cleveland 58 Columbus 28 Denver 60 Indianapolis 51 18,364,070 4 ,24 7,461 10 ,27 4,4 42 20,476,001 5,086,395 863,188 2, 744,344 4,599,718 546, 020 7,566,910 25 25 25 25 25 25 16,9 72, 651 4, 422 ,27 7 13 ,29 0,887 ... 29 9,9 02 79,083 33,515 1,843 19,695 21 4, 428 127 ,4 32 337 2, 158 1,0 72 Army 3,051 22 ,301 8,589 21 8 ,27 3 113,961 1,165,430 1,504 300,3 82 93,075 8,705 56,865 2, 429 23 , 829 24 0,068 33,995 27 4,913 437,944 23 7,694 ... October 1999, of the introduction of hourly billing The combination of our knowledge of DFAS expenditures and the exact timing of the introduction of the new billing system gives us the ability...
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Báo cáo khoa học: Comparative analysis of the site-specific N-glycosylation of human lactoferrin produced in maize and tobacco plants pdf
... Ó FEBS 20 03 323 6 B Samyn-Petit et al (Eur J Biochem 27 0) of the antennae, and the presence of a bisecting b1 ,2- xylose, and of an a1,3-fucose residue instead of a1,6-fucose, linked to the proximal ... deglycosylation of H2, M2 and T2 reveal one major peak corresponding to a [M + H]+ ion at m/z 323 2.05, 323 2.69 and 323 2.45, respectively This peak at 323 2 Da corresponds exactly to the first N-glycosylation ... 778.31, 721 .21 , 634 .22 , 519 .20 and 359.18 (Fig 4B) The mass increments between these peaks, i.e 57, 87, 115 and 160 Da, correspond exactly to the masses of glycine, serine, aspartic acid and carboxamidomethylated...
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a comparative analysis of the cognitive moral development of independent public auditors in selected public accounting firms and bank examiners in selected federal banking regulatory agencies
... Reproduced with permission of the copyright owner Further reproduction prohibited without permission Reproduced with permission of the copyright owner Further reproduction prohibited without ... permission Reproduced with permission of the copyright owner Further reproduction prohibited without permission Reproduced with permission of the copyright owner Further reproduction prohibited without ... permission Reproduced with permission of the copyright owner Further reproduction prohibited without permission Reproduced with permission of the copyright owner Further reproduction prohibited without...
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theoretical and empirical analysis of the economics of traceability adoption in food supply chains
... parts of the world, for example in the United States (US) and EU The second derives from the globalization of business and the increasing sophistication of food manufacturing and supply chains, ... because the more firms in a supply chain are willing to adopt it the more complete might be the level of information and the value of traceability in the chain When modeling supply chains using ... building and repairing airplanes Also, the structures of these industries are different Finally, building and repairing planes is much more complex than shipping mail or packages In the case of the...
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