... DNA strand’s ends are made blunt with appropriate enzymes • “A” and “B” adapters are ligated to the blunt ends using DNA ligase • The strands are denatured using sodium hydroxide to release the ... beads containing sulfurase and luciferase, and packing beads used only to keep the DNA beads in place • Above the wells is a flow channel, passing nucleotides and apyrase in a timed schedule ... The nucleotide bases are added in a timed fashion (beginning with A, T, G, C with 10s between each nucleotide and a successive apyrase wash, followed by the next nucleotide.) • As a bi-product...
... number of disease characteristics and body function parameters were controlled and that the HRQOL was measured using various instruments It was seen that the disease characteristics are generally ... was made and patient participation was not included until the last block of the model, it does appear to us to be an argument for the importance of patient-oriented communication style andfor ... results are compatible with intervention studies (e.g [52,53]) and observation studies [54], which show that for cardiac disease, Farin and Meder Health and Quality of Life Outcomes 2 010, 8 :100 http://www.hqlo.com/content/8/1 /100 ...
... 50-50, and 60 and older), and was used as a continuous variable in the survival analyses BMI was categorized at baseline and was categorized into categories ( < 25, 25-29, and >= 30 kg/m2), and was ... in this analysis were sex, age, body mass index (BMI), smoking status, race, and educational status Age was classified at baseline, the year 1, and year examinations and was categorized for use ... placebo, and Control Statistical Analysis Data analysis was completed using statistical software (Statistical Analysis Software, version 9.2; SAS Institute; Cary, NC and SUDAAN version 10. 1; RTI,...
... converters are of many types and can be classified as uncontrolled and controlled types [1] Uncontrolled rectifiers are based on power diodes and provide a constant DC output voltage for a fixed ... from phase locked loop λ time constant of tracking error λi time constant of tracking error for current loop λv time constant of tracking error for voltage loop µ small positive parameter for system ... parasitic resistance R, the basic circuit equation for the fundamental components in phase a can be expressed as, ea1 (t) = uan1 (t) + vaL1 (t) (1.4) The phasor form of (1.4) can be expressed as...
... All phase windings conduct current at the same time Back EMF Phase A 120o Phase B 240o Phase C (a) Back EMF 180o Phase A 120o Phase B 240o Phase C (b) Figure 1.1: Basic excitation waveform for ... interior PMSM 107 5.3 Standard stator and designed rotor for the prototype interior PMSM 107 5.4 Experimental set-up for the interior PMSM drive system 108 5.5 dSPACE DS 1102 based integrated ... PMSMs has been implemented for a smooth and accurate speed and torque control The advantages and disadvantages on the control performance were investigated through theoretical analysisand experimental...
... templates and10 pmole forward or reverse primers Thermocycling profile was set for denaturation at 96° C for 30 seconds, annealing at 50° C for 15 seconds, extension at 60° C for minutes and repeated ... precipitated DNA was subjected to centrifugation at 13 000 g for 21 minutes DNA pellet was washed with 500 µl of 70% ethanol Centrifugation was performed for another minutes and the supernatant was 31 removed ... IgG4 subclass, are believed to compete with human IgE for the allergen thus blocking IgE-dependent histamine release and the downstream acute phase responses Recognizing the same epitopes as human...
... guaranteed for the probabilistic components On the other hand CoNLL restricted itself to the easier attachment task and therefore provided the gold standard POS tag as part of the input data, whereas ... Basic algorithm for heuristic transformational search ATTR hier kletterte die Marke von 420 auf 570 Mark The final analysis correctly takes ‘570 Mark’ as the kernel of the last preposition, and ... sentences, and always delivers an analysis that contains exactly one subordination for each word, the common measures of precision, recall and f-score all coincide; all three are summarized as accuracy...
... financial leverage even further and borrow funds for more share repurchases The effect will generally be a rise in the value of the stock as fewer shares are left outstanding, and because of the proportional ... shares of common stock outstanding, and a defined number of rights are necessary to purchase each share of new stock Rights are issued as special certificates andare often traded on securities ... The lease term is for at least 75 percent of the asset’s economic life • The present value of the lease payments is at least 90% of the asset’s value From a tax standpoint, the periodic lease payments...
... System sectors are special areas on your disk containing programs that are executed when you boot (start) your PC System sectors (Master Boot Record and DOS Boot Record) are often targets for viruses ... and infect at later stages Attack Phase: • Some viruses have trigger events to activate and corrupt systems • Some viruses have bugs that replicate and perform activities like file deletion and ... is identifiedas threat infecting target systems Incorporation Elimination Anti-virus software developers assimilate defenses against the virus Users are advised to install anti-virus software...
... Cash dividends, 121 Cash flow analysis, 132, 435 Cash flow cycle, 435 Cash flow forecast, 435 Cash flow from operations, 167, 435 Cash flow multiples, 124, 401 Cash flow per share, 119–120 Cash ... transfers in the United States for payment abroad, and quotations are given for the current trading day as well asfor the previous day Also, prices for foreign bank notes are often quoted in equivalents ... shareholder return Share price appreciation The change in the market value of preferred and common shares over time Share repurchases (stock buyback) The purchase by a company of its own shares...
... They are kept under growing conditions for the chosen plants and the emergence and mass of the test plants are compared against those of control plants Theory The importance of plants as the basis ... Penetrating the soil and building burrows, as well as increasing pore space • Transporting soil and organic matter by casting 18 Assessment of Ecotoxicity of Contaminated Soil Using Bioassays 341 • Comminuting ... soil surface (mainly in forest ecosystems; Doube and Brown 1998), and to an increased yield in orchards or grassland (Blakemore 1997) The activities described above are performed by various earthworm...
... incidence was 10. 4 percent In contrast, 65.7 percent were located in the rural areas, where the poverty incidence was 54.3 percent Poverty Impact Analysis: Tools and Applications Chapter 10 325 ... simulation Poverty thresholds are available for the whole Philippines, urban and rural, andfor the 14 regions’ urban and rural areas The consumer price index is derived as the weighted composite ... weights are the shares of the households’ consumption basket from the various areas and regions See Ravallion (1992) for detailed discussion on this issue Poverty Impact Analysis: Tools and Applications...
... Confidence limits are 95% Wald confidence limits Disease duration was set as positive when present for at least months Morning stiffness was set as positive when it lasted for at least 60 The swollen ... Activity Score using 28 joints and variables (tender and swollen joint counts and CRP; DAS28-3) was calculated with the appropriate formulas http:/ /www.das-score.nl [40] A DAS28-3 score below 2.6 defined ... statistical analysis, and reviewed the draft manuscript ZJZ developed the anti-Sa assays and performed a large portion of the assays for the study CD participated in the design of the protocol and performed...
... softening temperature and Tg are related they are not equal, and a clear distinction should be made between them Many polymers are viscoelastic and recover elastically following deformation Figure ... and load (force) calibration; • exact location and type of sample thermocouple 6.3 Dilatometry Formerly dilatometry was commonly used to measure sample volume as a function of temperature Glass ... by a glass rod of length 2-3 cm which fits the inner diameter of the glass tube and acts as a spacer (step III) The glass tube containing the sample is sealed using a gas burner and the glass capillary...
... expressed as penetration force per unit cross-sectional area of the cone base Penetrometer resistance measurements are used widely, are relatively quick and easy to make, and can provide data that are ... base areas of 320 mm and 130 mm 2, both with a 30Њ cone angle A 3% decrease in diameter is allowed for cone wear In Europe, cones of 100 mm base area are common, but cones with base areas of up ... base area, it tends to increase with decreasing base area (Freitag, 1968) For field penetrometers, the standard of the American Society of Agricultural Engineers (ASAE, 1969) allows cone base areas...
... 95°C for minutes; 35 cycles of 95°C for 30 s, 60°C for 15 s and 74°C for 15 s; and 74°C for10 minutes for final extension The PCR product was incubated with NcoI (New England Biolabs) and digested, ... at 101 and 38 bp identified those who were GG homozygous, and three bands at 139, 101 and 38 bp indicated a heterozygote at the -108 2 locus Statistical analysis The main outcome measure was in-hospital ... mmol/l MgCl2 and U Taq polymerase Amplification was performed with an initial denaturation of 95°C for minutes; followed by 35 cycles of 94°C for 30 s, 69°C for 30 s and 74°C for 42 s; and completing...
... element performance? What are common methods for assessing SYSTEM and element performance? What are the key SE focus areas for current system performance? What are the key SE focus areas for planning ... Performance • • • • • Task 2.1 Task 2.2 Task 2.3 Task 2.4 Task 2.5 Perform informal testing Evaluate informal test results Optimize design and test article performance Prepare test article for formal ... referred to asanalysis paralysis These areas are prime example As a reality check, SEs need to ask themselves the question: If we simply asked Users to identify and prioritize to system areas for improvement,...
... index MAS microarray production was performed as previously described [24,26,29] using the sequences of chromosome 10for japonica and indica rice as were available on 12 April, 2004 [8] and August, ... false and not represent real genes For further analysis, gene models were classified as high homology (HH) and low homology (LH) models based on comparison using an expect value of e-7 for predicted ... Cloning (see followinganalysis of japonica chromosome 10 UG models and intergenic TARs Figure and sequence page) Cloning and sequence analysis of japonica chromosome 10 UG models and intergenic TARs...