adipose derived stem cells characterization and application in urology

... Kilroy G, Wu X, Gimble JM Immunophenotype of human adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers Stem Cells 2006, 24, 376-385 26 Muir WW 3rd, Wiese ... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stem cells ... EW, Park YH Soluble factors-mediated immunomodulatory effects of canine adipose tissue -derived mesenchymal stem cells Stem Cells Dev 2008, 17, 681-693 Kang SK, Lee DH, Bae YC, Kim HK, Baik SY,...

Ngày tải lên: 07/08/2014, 23:22

... cells and the host tissue As compared with embryonic stems cells and bone marrow -derived mesenchymal stem cells, adiposederived mesenchymal stem cells (ADMSCs) have the distinct advantages of being ... J, Ratajczak MZ: Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis Stem Cells 2005, 23:879-894 Kee HJ, Sohn IS, ... Page of 16 and and between groups and (Figure 4A) However, this protein expression was substantially lower in group than in other groups and notably lower in groups and than in groups and (Figure...

Ngày tải lên: 18/06/2014, 16:20

... the staff of Miplant Stems Clinic Authors’ contributions JP was in charge of patient treatment and follow-up, was responsible for manuscript drafting and revision, and read and approved the final ... 367(Suppl):S244-S253 Barry FP: Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derived stem cells repair the defect of ... Enzymol 1982, 82:453-471 14 Salem H, Thiemermann C: Mesenchymal stromal cells: current understanding and clinical status Stem Cells 2010, 28:585-596 15 Richardson DW, Dodge GR: Dose dependant effects...

Ngày tải lên: 10/08/2014, 23:21

... show that human adipose tissue -derived mesenchymal stem cells (AT-MSCs) differentiate into hepatocyte-like cells upon treatment with hepatocyte growth factor (HGF), oncostatin M and dimethyl sulfoxide ... Huang CT, Tsai CC, Shen EY & Chiu WT (2005) Isolation and characterization of neurogenic mesenchymal stem cells in human scalp tissue Stem Cells 23, 1012–1020 In’t Anker PS, Scherjon SA, Kleijburg-van ... mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stem cells...

Ngày tải lên: 30/03/2014, 04:20

... expanded with and without FGF-2 stained strongly for CD13, CD44, CD90, and CD105 (markers for mesenchymal stem cells) and for CD29 (β1 integrin) (Figure 3) The cells stained poorly for LNGFR and ... IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cells and of epitopes commonly abundant on adult stem cells derived ... stem cells [48] FGF2 is routinely used in the culture of bone marrow -derived mesenchymal stem cells, and we have determined a baseline for the use of FGF-2 in the culture of fat pad -derived cells...

Ngày tải lên: 09/08/2014, 10:23

... V-FITC and pyridine iodination (PI) co-staining and analyzed by flow cytometry Q3 cells were regarded as control cells, whereas Q4 cells were considered as a measure of early apoptosis, Q2 cells ... activation of caspases-9 and -3 and apoptosis (A) The effects of HSP70 and its deletion mutant proteins on the activation of caspases-9 and -3 were analyzed Cells over-expressing HSP70 and its deletion ... between HSP70 and Smac Vector control (C2C12-C) and HSP70-over-expressing (C2C12-HSP70) cells were either kept untreated or treated with 0.5 mM of H2O2 for h Cells were harvested and lysed Next,...

Ngày tải lên: 16/03/2014, 01:20

... was 65.45% and the purified degree was 3.03 This purified protease was used for determination of Km, Vmax and applied in fish sauce processing 2.3 Determination of kinetic parameters Km and Vmax ... ninhydrin reagent RESULTS AND DISCUSSION Our prelimenary study shows that the optimal pH and temperature of the protease produced by Aspergillus oryzae surface culture are 7.0 and 50oC respectively ... with NaCl 50g/L and 100g/L, the enzyme activity remained 70% and 50% in comparison with the control sample.[2] 3.1 Influence of different ions to the protease activity Figures and show the influence...

Ngày tải lên: 22/07/2014, 10:21

... the stem cells and/ or attract the endogenous or exogenous stem cells (endogenous from the bone marrow and exogenous by multiple intra-articular injection of the expanded autologous stem cells) ... MD Fibronectin, vitronectin, and collagen I induce chemotaxis and haptotaxis of human and rabbit mesenchymal stem cells in a standardized transmembrane assay Stem Cells Dev 2007 Jun;16(3):489-502 ... mesenchymal stem cells Science 1999 Apr 2;284(5411):143-7 PubMed PMID: 10102814 Epub 1999/04/02 eng 65 Deasy BM, Jankowski RJ, Huard J Muscle -derived stem cells: characterization and potential...

Ngày tải lên: 10/09/2015, 09:01

... cells Pancreatic stem cells Intraislet, nestin-positive cells, oval cells, duct cells Beta cells Skletal-muscle stem cells or satellite cells Muscle fibers Skeletal muscle fibers Stem cells of the ... MSCs), adipose derived stem cells (ADSCs), periostal cells, skeletal muscle and synovial fibroblasts derived cells (63-68), 12   which we will not discuss about them in this chapter 1.4.5.3 Stem Cells ... 1.4.5.3 Stem Cells in Articular Cartilage repair 13   iv   1.5 Types of stem cells 14   1.5.1 Embryonic stem cells (ESCs) 15   1.5.2 Induced pluripotent stem (iPS) cells 15   1.5.3 Adult stem cells...

Ngày tải lên: 10/09/2015, 09:10

... Figure 3-3 Flow cytometry analysis of the stem cells surface markers Harvested cells were positive for CD29, CD44, and CD90, and negative for CD14, CD31, CD45, and CD34   59   3.4.2 Prussian blue ... the cells were positive for MSC characteristics, such as adhesion molecules (CD29, CD44, and CD90), more than 98% of cells were negative for hematopoietic markers (CD14, CD31, CD34, and CD45), and ... closed and rested for one week MSCs (107 cells) labeled with 50µg/ml ferucarbotran (10pg/cell) were seeded in ml hyaluronan and used for intra-articular injection of the experimental knee and ml...

Ngày tải lên: 10/09/2015, 09:10

... and scaffold only defect (A), 100 cells simulation (B), 1000 cells simulation (C), and 100,000 cells simulation (D) Left panel of each image is 3D-FSE sequence and Right panel is 3D SPGR sequence ... showed that the distribution of collagen type II in extracellular matrix of cells was same between unlabeled and 25 and 50 µg/mL SPIO labeling, however the matrix production were decreased in ... for anatomical data and the sensitivity of gradient echo sequences (SPGR and 2D/3D-GRE) to signal loss due to iron nanoparticles to reveal contrast between newly formed tissue and the incorporated...

Ngày tải lên: 10/09/2015, 09:11

... indicate the defect site and arrows demonstrate the labeled stem cells) 72     Figure 3-16 MRI of the distribution of the labeled MSCs in the mini-pig knee joint The labeled cells migrated to the ... healing process and the secretion of proinflammation factors associated with remodeling and repair of the injury provided the stimuli for MSC migration MRI revealed that the injected cells were in ... knee (figure 3.14) The labeled cells migrated to different locations over time including cartilage defect, injection site, surgery scar, and supra-patellar fat and the cartilage defect site It...

Ngày tải lên: 10/09/2015, 09:11

Ngày tải lên: 10/09/2015, 09:11

... method of the cells can be a promising tool for real time evaluation and tracking of MSCs, providing data for a better understanding of the migration and localization of the injected cells 76   ... viability and chondrogenic differentiation of the labeled cells at higher concentrations Our results are in agreement with those of Kostura et al (192), but not with those of Heymer et al (205) and ... loading of the cells (5 pg iron per cell) by Heymer et al., which is similar to our cells iron content labeled with lower labeling concentration (25–50 µg/mL) Hence, an optimized and controlled...

Ngày tải lên: 10/09/2015, 09:11

... Grand Island, NY, US) and sub-cultured into two new flasks Cells were expanded and sub-cultured to get enough number of cells for the further experiments (passage 4) The cells were detached and ... software, and an intensity threshold level was established for discrimination between migrated cells and the background The masks of the outlined cells (cells masks) were plotted and displayed ... CD44 and CD90 and negative for hematopoietic markers CD14, CD34 and CD45, and endothelial marker CD31 (Figure 4.5) In addition cells could differentiate to all three adipogenic, osteogenic, and...

Ngày tải lên: 10/09/2015, 09:11

... performance of stand‐ alone conventional and inverted organic solar cells, and a further discussion about optimiz‐ ing organic tandem solar cells by considering the current matching of the sub -cells At ... Menossi, Alessandro Romeo and Nicola Romeo Chapter Cu2ZnSnS4 Thin Film Solar Cells: Present Status and Future Prospects 107 Minlin Jiang and Xingzhong Yan Chapter Thin Film Solar Cells Using Earth-Abundant ... ferent band of the solar spectrum Si-based tandems based on quantum dots (QDs) and quantum wires (SiNWs) allowing band gap engineering and their optimization methods in‐ fluencing their optical and...

Ngày tải lên: 16/03/2014, 12:20

... 18.2 12.7 19.8 12.4 JPcF – – 936.2 934.0 – – the states A and B and the exchange rates k1 for A fi B and k)1 for B fi A As B ¼ 100%–A and k)1 ¼ k1*A/B only two free parameters had to be fitted to ... spectra (a-, b- and c-phosphorus nuclei), using chemical shift values and J-coupling constants as listed in Tables and Isothermal titration calorimetry The interaction between a nucleotide and the magnesium ... synthesis of the two guanosine triphosphate nucleotide analogues GTPcNH2 and GTPcF, their biochemical characterization and application to the GTP-binding protein Ras GTPcNH2 was prepared by the...

Ngày tải lên: 18/03/2014, 01:20

... Studying Stem Cells and Progenitors Jane E Aubin, Fina Liu, and G Antonio Candeliere 403 30 Nonradioactive Labeling and Detection of mRNAs Hybridized onto Nucleic Acid cDNA Arrays Thorsten Hoevel and ... (ES cells to inject and cells to pellet for DNA) into the microfuge and spin for at 110g To make sure trypsin is removed, aspirate the supernatant and add mL M2 to the cells for injection and ... to use ES cells for lineages not yet being widely studied with this model and to develop new methodologies that would contribute to both the fundamental understanding of stem cells and their...

Ngày tải lên: 08/04/2014, 12:51

... section, “Utilization /Characterization of Neural Stem Cells in vivo,” is a collection of techniques to identify and characterize endogenous stem cells as well as exogenous stem cells after transplantation ... and Lois, C (1995) Neuronal stem cells in the brain of adult vertebrates Stem Cells 13, 263–272 Production and Analysis of Neurospheres 27 Gage, F., Ray, J and Fisher, L (1995) Isolation, characterization, ... astrocytes are neural stem cells in the adult mammalian brain Cell 97, 703–716 Stem Cells, Neuroblasts, and Glioblasts from Fetal Tissue 29 Isolation of Stem and Precursor Cells from Fetal Tissue...

Ngày tải lên: 11/04/2014, 09:58

... ash content and total glycerol content, were determined and listed in the Table Table also showed comparisons of the obtained biodiesel and the standards of biodiesel in china, Europe and the United ... (mixture of catalyst and reactants) was become too viscous giving rise to a problem of mixing and a demand of higher power consumption for adequate stirring On the other hand, when the catalyst ... lifetime and better stability than current homogeneous catalysts It is noncorrosive and environmentally benign It can be applied to produce biodiesel commercially 3.4 Characterization and properties...

Ngày tải lên: 05/05/2014, 08:42