automated isolation and processing of adipose derived stem and regenerative cells

... system will provide a valuable tool, in addition to primary hepatocytes, for study of the molecular basis of the regenerative and developmental processes of hepatic cells in vitro Isolation of ... mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stem cells ... determining stem cell transdifferentiation Discussion AT-MSCs may be good candidates as stem cells for cell transplantation and tissue engineering in regenerative medicine, as a large number of AT-MSCs...

Ngày tải lên: 30/03/2014, 04:20

... Immunophenotype of human adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers Stem Cells 2006, 24, 376-385 26 Muir WW 3rd, Wiese AJ, March PA Effects of morphine, ... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stem cells ... immunomodulatory effects of canine adipose tissue -derived mesenchymal stem cells Stem Cells Dev 2008, 17, 681-693 Kang SK, Lee DH, Bae YC, Kim HK, Baik SY, Jung JS Improvement of neurological deficits...

Ngày tải lên: 07/08/2014, 23:22

... of stem cells and precursor cells for this purpose The two types of stem cells that are being targeted for use in tissue regeneration are the Embryonic stem cells and the Adult stem cells The ... source of multipotent adult stem cells is the adipose tissue Cells isolated from these, known as ADSCs (Adipose Derived Stromal Cells) have been shown to possess the property of being multipotent and ... encompassing microvascular endothelial cells and even smooth muscle cells and a type of precursor cells called Adipose tissue derived cells (ADSCs) (Zuk PA et al., 2001) These cells have variably been known...

Ngày tải lên: 08/11/2015, 17:01

... efficient isolation and systematic identification of human adipose- derived mesenchymal stem cells Journal of Biomedical Science 2011 18:59 Submit your next manuscript to BioMed Central and take ... differentiation of cryopreserved human adipose- derived stem cells Cryobiology 2008, 57:18-24 Chen MY, Lie PC, Li ZL, Wei X: Endothelial differentiation of Wharton’s jelly -derived mesenchymal stem cells ... enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of protein by lipolytic...

Ngày tải lên: 10/08/2014, 10:20

... CHARACTERIZATION OF HUMAN ADIPOSE DERIVED ADULT MULTIPOTENT PRECURSOR CELLS LEONG TAI WEI DAVID (Bachelor of Chemical Engineering, Hons) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... bone healing or regenerative intervention 1.1.2 The use of stem cells in regeneration of bone 1-1 1-1 1-2 Chapter – Literature Review 2.1 Description of the adipose tissue 2.2 Adipose tissue – ... downstream expression of a stemness gene in adipose derived stem cells 9.1 Chapter Abstract 9-1 9.2 Introduction 9-2 9.3 Specific Materials And Methods 9-7 9.4 Results And Discussion 9-13 9.5...

Ngày tải lên: 12/09/2015, 09:55

... loosely adherent cells, and ml per well of new AIM-V medium containing 560 u/ml of recombinant human GM-CSF (Research Diagnostic Inc., Flanders, NJ) and 25 ng/ml of IL-4 (R&D Systems, http://www.retrovirology.com/content/1/1/37 ... added and the cells were cultured at 37°C and 5% CO2 On Day 3, ml of fresh AIM-V medium containing 560 u/ml of GM-CSF and 25 ng/ml of IL-4 was added to the culture On Day 5, the non-adherent cells ... transduction of DCs and analysis of surface marker expression LV transduction of DCs and analysis of surface marker expression PBMC -derived DCs were infected with LV on Day (d5) and after maturation,...

Ngày tải lên: 13/08/2014, 13:20

... benign samples PB performed stem cell isolations from tumors CH performed culturing and isolation of stem cells, and NF-κB experiments JLL performed the RTPCR detection of the TMPRSS:ERG fusion ... cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells were ... further study of larger samples of prostate cancer stem cells from different classes of therapy-resistant and Gleason tumors is warranted Despite short-term culturing, to expand the stem cell population,...

Ngày tải lên: 14/08/2014, 08:21

... (p-values of < 0.05) unless stated otherwise 49 Results 4.1 Isolation of HPTC and characterization of freshly isolated and commercial HPTC 4.1.1 Isolation of HPTC HPTC are available commercially and ... Isolation of HPTC and characterization of isolated and commercial HPTC 50 4.1.1 Isolation of HPTC 50 4.1.2 Characterization of HPTC by immunoblotting 51 4.1.3 Characterization of ... containing 0.1 mM of 3-isobutyl-1-methylxanthine (IBMX) (Merck) and exposure of cells to 100 nM and 1000 nM of human PTH (Sigma-Aldrich) and human AVP (SigmaAldrich) for 30 minutes at 37°C The cells were...

Ngày tải lên: 09/09/2015, 10:19

... osteogenic differentiation of 49 adipose derived stem cells 4.2.5 Hepatic differentiation of adipose derived stem 51 cells 4.2.6 Pro-angiogenic activity of adipose derived stem 53 cell – gelatin ... regeneration 2.2.1 Embryonic stem cells 2.2.2 Adult stem cells 2.2.3 Adipose derived stem cells Chapter Chapter v 2.2.4 Hepatic differentiation of adipose derived stem 10 cells 2.2.5 Characterization ... of the advent of a variety of adult stem cells and the kind of impact that such therapies can create on the status quo medical treatments Adult stem cells, unlike embryonic stem cells, are not...

Ngày tải lên: 09/09/2015, 11:18

... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... from the staff of Miplant Stems Clinic Authors’ contributions JP was in charge of patient treatment and follow-up, was responsible for manuscript drafting and revision, and read and approved the ... Mesenchymal stromal cells: current understanding and clinical status Stem Cells 2010, 28:585-596 15 Richardson DW, Dodge GR: Dose dependant effects of corticosteroids on the expression of matrix-related...

Ngày tải lên: 10/08/2014, 23:21

... CD11b+) showed an abundance of immature cells, including metamyelocytes or band cells and blast-like cells (middle left and far right panels, representative images shown of three independent experiments) ... level of both CD4+FOXP3+ regulatory T cells and Page 20 of 20 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 CD14+HLA-DR-/low myeloid -derived suppressor cells and decreased level of dendritic ... GraphPad Prism software (La Jolla, CA) with a significance level of 0.05 Graphs and figures were produced using GraphPad Prism, Microsoft Excel, and Adobe Illustrator and Photoshop software (San...

Ngày tải lên: 18/06/2014, 19:20

... may be derived from subsequent isolation and re-plating of the ICM cells, which are then screened for stemness by a variety of recognized markers Once in stable culture, ESCs are capable of either ... Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human ... further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole embryos or compromising...

Ngày tải lên: 13/08/2014, 23:20

... embryonic, fetal/neonatal and adult I will review first embryonic stem cells, induced pluripotent stem cells and then the adult bone marrow derived stem cells, which are well known and best studied for ... characterization of definitive MSC markers 2.4 Fetal/neonatal stem cells 2.4.1 Background Fetal and neonatal stem cells come in between embryonic and adult stem cells in the developmental continuum Studies of ... from abortions, and use of fetal stem cells has been 20 the subject of some ethical debate In this review, discussion of fetal stem cells will be limited to amniotic fluid stem cells, which can...

Ngày tải lên: 11/09/2015, 10:02

... experience of our research group in the identification, and characterization of potential sources of adult stem cells [7,10,1215], the aim of this study was to isolate, expand, characterize and assess ... new potential source of pluripotent cells for regenerative medicine The identification of niches of tissue-specific stem cells capable of replacing damaged differentiated cells in the hFTs may ... Vainzof M, Zatz M: SJL dystrophic mice express a significant amount of human muscle proteins following systemic delivery of human adipose- derived stromal cells without immunosuppression Stem Cells...

Ngày tải lên: 18/06/2014, 15:20

... staining of IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cells and of epitopes commonly abundant on adult stem cells ... stem cells [48] FGF2 is routinely used in the culture of bone marrow -derived mesenchymal stem cells, and we have determined a baseline for the use of FGF-2 in the culture of fat pad -derived cells ... Clonal IPFP -derived cells retained the cell surface characteristics of the parent IPFP cells, which were similar to mesenchymal stem cells The clonal populations of IPFP -derived cells appeared...

Ngày tải lên: 09/08/2014, 10:23

... and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and growth ... x 105 Figure cells with an HIV-EGFP vector at a moi = 0.5 Infection of parental and mutant HeLa cells (30-2 and 42-7) Infection of parental and mutant HeLa cells (30-2 and 42-7) cells with an ... fractionation of cytoplasmic and nuclear extracts and measurement of the amount of full product 36 hours after infection in parental (black bars) and 42-7 (grey bars) cells; (E) Parental and 42-7 cells...

Ngày tải lên: 13/08/2014, 05:22

... 400 µl of suspension are added to 400 µl of PBS containing the known concentration of the drug and 2.5 mmol of ATP, 2.5 mmol MgCl2 and 2.5 mmol of CaCl2, gently mixing to avoid hemolysis and incubation ... endocytosis and osmosis-based systems or by chemical perturbation of the erythrocytes membrane [16] Endocytosis is the process by which cells absorb molecules by engulfing them It is used by all cells of ... efficiency and cell recovery) [25] Loaded amount The total amount of pravastatin entrapped in 0.1 ml of the final packed erythrocytes Efficiency of entrapment The percentage of the loaded amount of pravastatin...

Ngày tải lên: 25/10/2012, 11:10

... electrophoresis of the PCR product indicated the presence of two distinct bands between 1000 and 1100 bp of roughly the same extent (not shown) Cloning and nucleotide sequencing of these two products ... the mean of three independent experiments and are presented as copies of the target variant per 109 copies of 18S RNA Quantitation of ACMSD variants in liver, kidney and brain An isoform-specific ... the software swiss-pdbviewer and visual molecular dynamics [49] Acknowledgements We thank G Magni and S Ruggieri for helpful comments and suggestions and T Begley (Department of Chemistry and...

Ngày tải lên: 19/02/2014, 02:20