acinetobacter yersinia serratia and burkholderia lps
Báo cáo y học: "Binding of protegrin-1 to Pseudomonas aeruginosa and Burkholderia cepacia" potx
... representative, we prepared LPS and lipid A from another pair of bacteria (P aer- uginosa FRD-2 and B cepacia C4813) and repeated the studies The EC50 values for both LPS and lipid A obtained from ... supernatant and phthalate oil, the base of the tube was severed with a razor blade and the stub, which contained the pellet, was recovered for radioactivity counting LPS and lipid A extraction and analysis ... than that of P aeruginosa (4.54 µm2) Binding of PG-1 to LPS and lipid A To compare binding of PG-1 to LPS and lipid A from P aeruginosa 9027 and B cepacia 25416, we devised a simple Page of 11 (page...
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Tài liệu Báo cáo khoa học: Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx
... PAGE gel and Coomassie Blue staining, and the band corresponding to a modified OTUB1 (rectangle) was excised and digested with trypsin The peptide mixture was analysed by a nano-UPLC-QTOF tandem ... and OTUB1–probe adduct were visualized by immunoblotting using HA antibodies and quantified for two experiments (black and grey bars) The intensities of the corresponding bands were measured and ... increased Yersinia uptake We therefore tested whether YpkA and RhoA interact in vitro and whether this protein complex includes OTUB1 YpkA was immunoprecipitated and the presence of OTUB1 and RhoA...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of Asterisks and broken line: data and fitting with ... nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and averaged ... 12768–12785 Pulver, S.C., Froland, W.A., Lipscomb, J.D & Solomon, E.I (1997) Ligand field circular dichroism and magnetic circular dichroism studies of component B and substrate binding to the...
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Báo cáo khoa học: CpG and LPS can interfere negatively with prion clearance in macrophage and microglial cells pdf
... co LPS CpG GpC co LPS CpG GpC co LPS CpG GpC 0h 30 22L 21 10 11 12 J774 B 2d 7d 5d co LPS CpG GpC co LPS CpG GpC co LPS CpG GpC co LPS CpG GpC 0d 30 22L 21 101112 13 14 15 16 24 h 48 h co LPS ... 12 CO LPS CpG GpC 12 hours 18 24 0h J774 3,5 * ** * ** ns ns 2,5 J774 B 24 h 48 h co LPS CpG GpC co LPS CpG GpC co LPS CpG GpC arbitrary units 48 h 30 0,5 30 RML * ** ns * 21 1,5 0,5 0 CO LPS CpG ... directly after infection (lanes and 3) This finding was reproducible and was not the case if nonstimulatory LPS (data not shown) or GpC-ODN (lane and 12) was applied In LPS- stimulated samples, a pronounced...
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Báo cáo khoa học: Degradation of chitosans with chitinase B from Serratia marcescens Production of chito-oligosaccharides and insight into enzyme processivity docx
... a DP of 3.04 and a FA of 0.44, and was concluded to consist mainly of DDA and DAA, in addition to minor amounts of ADA The tetramer fraction showed a DP of 4.1 and a FA of 0.33 and the dominant ... same unit resonates at 4.74.8 p.p.m [33] D- and A-units within the chain and at nonreducing ends resonate near 4.9 and 4.6 p.p.m., respectively [9] -AAb and -DAb are the reducing end b-anomer where ... diad frequencies and block lengths of each chitosan are compared with the calculated diad frequencies and block lengths of a chitosan with a random (Bernoullian) distribution of A- and D-units (numbers...
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Báo cáo khoa học: Role of Acinetobacter calcoaceticus 3,4-dihydrocoumarin hydrolase in oxidative stress defence against peroxoacids Kohsuke Honda, Michihiko Kataoka, Eiji Sakuradani and Sakayu Shimizu doc
... and the physiological role of the enzyme Materials and methods Chemicals and enzymes A stock solution of peracetic acid of 32% (w/v) concentration in equilibrium with < 6% hydrogen peroxide and ... phosphatase (calf intestine), T4 DNA ligase and Ex Taq DNA polymerase were from Enzyme assay, SDS/PAGE and protein determination Acinetobacter strains and the recombinant E coli strains were cultured ... site (in italics), and dch-II (ATGTAAGATCTGA ACTGGACTTCG) containing a BglII site (in italics), cloned into pT7Blue, excised with EcoRI and BglII, and then replaced at the EcoRI and BglII sites...
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Báo cáo Y học: A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis pptx
... structural and sequence homology with EcCM, the active site residues of 90-MtCM can be identified, and are shown in Fig The striking difference between EcCM and 90MtCM is that the EcCM residues Ser84 and ... none of these residues is a candidate for performing the role of Gln88 in EcCM Of the other two structures, PfCM has the conserved Ser70 and Gln74, and TtCM has Ser81 and Glu85 There are two Protein ... (*EhCM) [16], *MtCM [5-7], and *YpCM; and AroH class CMs such as Bacillus subtilis (BsCM) [18]’ was corrected to ‘Erwinia herbicola CM (*EhCM) [16], *MtCM [5-7], and *YpCM; and AroH class CMs such...
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Báo cáo Y học: SMAP-29 has two LPS-binding sites and a central hinge docx
... between 10 and 16 was calculated for the control sample (containing peptide but no LPS) , and from this value the DD between 10 and 16 for the experimental samples, which contained peptide plus LPS, ... sensitive to LPS; the presence of multiple LPS- binding sites on a single Factor C molecule and their high positive cooperativity in LPS binding Although the affinity of SMAP-29 or CAP-18 for LPS we ... its shape drastically FTIR studies in trifluoroethanol buffer and LPS The helical structure of SMAP-29 in trifluoroethanol buffer and LPS micelles was further characterized using FTIR In the trifluoroethanol...
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Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 docx
... instructions (Bio-Rad) The anode and cathode buffers were mM phosphoric acid and 20 mM lysine/20 mM arginine, respectively IEF standards were purchased from Sigma and consisted of trypsin inhibitor ... Tris/HCl/2 mM MgCl2, with and without addition of mM CaCl2 and incubated at 30 °C During a 30-h period, samples in triplicate were randomly selected at times indicated and examined for lipase activity ... water miscible solvents (15% and 30%, v/v) in 30 mM Tris/HCl (pH 8.0) for h at 30 °C Activity remaining (%) was measured under standard conditions RESULTS AND DISCUSSION Acinetobacter sp RAG-1 has...
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Báo cáo khoa học: NMR and MS evidences for a random assembled O-specific chain structure in the LPS of the bacterium Xanthomonas campestris pv. Vitians A case of unsystematic biosynthetic polymerization potx
... phi and psi angles are defined by H1–C1–O1–CX and C1–O1–CX–HX, respectively, where X is the position of glycosylation RESULTS Isolation, characterization of the LPS and isolation of the OPS The LPS ... constant H1 to H2, between 0.5 and Hz, and the presence of the nuclear Compositional, size of ring and linkage analysis GLC-MS analysis of the acetylated O-methyl glycosides and of the acetylated (S)-2-octyl ... types of spin systems (A and A) were both endorsed as a-Rha residues (1JC,H ¼ 174 Hz and JH1-H2 ¼ Hz) The anomeric protons were present at 5.3 p.p.m and 5.1 p.p.m and correlated to two different...
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Báo cáo Y học: Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia potx
... an index with values of )1, and +1, which corresponds to upfield, random coil and downfield, respectively This image was constructed using the VINCE software (The Rowland Institute for Science, ... Yop synthesis in Yersinia pseudotuberculosis Mol Microbiol 42, 1075–1094 15 Anderson, D.M., Ramamurthi, K.S., Tam, C & Schneewind, O (2002) YopD and LcrH regulate expression of Yersinia enterocolitica ... used for resonance assignments and structure elucidation included phase sensitive DQF-COSY [23], TOCSY [24], NOESY [25] and gradient enhanced HSQC [26] In TOCSY and NOESY experiments the solvent...
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báo cáo hóa học: " 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranoside (AICAR) attenuates the expression of LPS- and Aβ peptide-induced inflammatory mediators in astroglia" pptx
... immunoblots for p-Pkb/Akt proteins Samples (1 and 7) correspond to control, (2 and 8), (3 and 9), (4 and 10), (5 and 11), (6 and 12) correspond to cells treated with LPS + Aβ +/- mM AICAR for 15 min, 30 ... subunits) and Figure C to phosphorylated AMPK (Ser 108) of AMPK β subunit In Figures B and C samples (1) control (2 and 5), (3 and 6) and (4 and 7) correspond to cells treated with LPS + Aβ peptide ... data for iNOS and MnSOD, and for all phosphorylation blots are expressed on an arbitrary scale Results AICAR attenuates LPS- and Aβ peptide-induced expression of cytokines and iNOS, and NO production...
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báo cáo hóa học: " Aspirin-triggered lipoxin A4 attenuates LPSinduced pro-inflammatory responses by inhibiting activation of NF-B and MAPKs in BV-2 microglial cells" pptx
... IL-1b and TNF-a, and their mRNA expressions; induced by LPS in BV-2 cells Consistent with our findings, similar results have shown that LXA4 and ATL inhibit LPS- induced production of IL-1b and ... of LPS- induced pro-inflammatory cytokines IL-1b and TNF-a using ELISA As shown in Figure 2B and 2C, stimulation of BV-2 cells with LPS led to a significant increase in the levels of IL-1b and ... JNK phosphorylation without effect on ERK and p38 MAPK activity ATL inhibits LPS- induced ERK and p38 MAPK activation ATL inhibits LPS- induced NF-B and AP-1 DNA binding activity Along with NF-B,...
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Báo cáo khoa học: " Dispersal and flight behaviour of lps sexdentatus (Coleoptera: Scolytidae) in pine forest" pptx
... (1963) lps confusus can fly up to km and Dendroctonus frontalis km Likewise Trypodendron lineatum can fly km (Shore and McLean, 1988), and lps typographus from 20-60 km (Nilssen, 1984; Forsse and ... of total recapture of Scolytus multistriatus and Lindelöw and Weslien (1986) and Salom and McLean (1989) found 90% respectively with lps typographus and Trypodendron lineatum These findings are ... pheromone-associated behaviour and physiology of lps pini Ann Entomol Soc Am 67, 58-60 PF (1982) Dispersal and flight behavior of the spruce bark beetle lps typographus in relation to sex, size and fat content...
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Báo cáo y học: "Suppression of LPS-induced matrixmetalloproteinase responses in macrophages exposed to phenytoin and its metabolite, 5-(p-hydroxyphenyl-), 5-phenylhydantoin" pptx
... contributions RS, NA and SN contributed to the concept and design of the study, and to the manuscript writing SN, RS and AA performed isolated of monocytes and culture of macrophages RS and AA performed ... PHT and HPPH inhibit LPS- induced supernatant levels of MMP-1, MMP-3, MMP-9, and TIMP-1 in a dose dependent manner To evaluate the effects of PHT and its metabolite, HPPH on macrophage MMP and ... were noted in the viability of cells exposed to LPS and either dose of PHT, HPPH, PHT /LPS or HPPH/ LPS as determined by MTS assay Further, we standardized the results of each analyte to total...
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Báo cáo y học: " Suppression of LPS-induced matrixmetalloproteinase responses in macrophages exposed to phenytoin and its metabolite, 5-(p-hydroxyphenyl-), 5-phenylhydantoin" pptx
... contributions RS, NA and SN contributed to the concept and design of the study, and to the manuscript writing SN, RS and AA performed isolated of monocytes and culture of macrophages RS and AA performed ... PHT and HPPH inhibit LPS- induced supernatant levels of MMP-1, MMP-3, MMP-9, and TIMP-1 in a dose dependent manner To evaluate the effects of PHT and its metabolite, HPPH on macrophage MMP and ... were noted in the viability of cells exposed to LPS and either dose of PHT, HPPH, PHT /LPS or HPPH/ LPS as determined by MTS assay Further, we standardized the results of each analyte to total...
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Báo cáo y học: "A novel hybrid aspirin-NO-releasing compound inhibits TNFalpha release from LPS-activated human monocytes and macrophages" docx
... Gliotoxin + LPS Gliotoxin B8 100μM + LPS B8 100μM B8 20μM + LPS B8 20μM LPS Media DMSO a I B Gliotoxin + LPS Gliotoxin B8 100μM +LPS B8 100μM B8 20μM + LPS B8 20μM B8 10μM B8 10μM + LPS B8 1μM + LPS ... written and the experiments were designed by CMT and AGR CMT performed the TNF-α release, LDH assay and immunofluorescence experiments, PM performed the western blot experiments and TAS performed and ... a 45 LPS stimulus Image c shows a monocyte treated with gliotoxin (0.1 μg/ml, 30 min) and stimulated with LPS (10 ng/ml, 45 min) Image d shows a monocyte treated with B8 (20 μM, 30 min) and stimulated...
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Báo cáo y học: "Potential mechanisms underlying the acute lung dysfunction and bacterial extrapulmonary dissemination during Burkholderia cenocepacia respiratory infection" pptx
... coagulation and of the fibrinolytic system [24] Prominent among the proinflammatory stimuli known to modulate TF expression in monocyte and endothelial cells is bacterial LPS [25] LPS was also ... Marques EA: Transient isolation of Burkholderia multivorans and Burkholderia cenocepacia from a Brazilian cystic fibrosis patient chronically colonized with Burkholderia vietnamiensis J Cystic ... Haemost 2004, 92:529-540 Zughaier SM, Ryley HC, Jackson SK: LPS from Burkholderia cepacia is more active than LPS from Pseudomonas aeruginosa and Stenotrophomonas maltophilia in stimulating TNF-a from...
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Báo cáo y học: " Resolution of LPS-induced airway inflammation and goblet cell hyperplasia is independent of IL-18 J Foster Harris1, Jay Aden1, C Rick Lyons2 and " doc
... inflammatory cells at days and d was lower in LPS instilled rats compared to those instilled with LPS and treated with IgG1 and anti-IL-18 antibodies While the numbers of macrophages and lymphocytes remained ... saline and returned to their cages After h, all rats were re-anesthetized and instilled with 1000 μg LPS Rats received i.p injections of 10 μg anti-IL-18 or IgG1 on days 1, 3, 5, and 7, and were ... period post LPS instillation The total inflammatory cells in the BALF reached maximum levels at d and post LPS instillation and returned to background levels by d 40 post instillation of LPS (Fig...
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