5chromatin remodeling and kinetics of nf kappa b activation a complex interplay

Báo cáo hóa học: " Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue" pdf

... 5'-CTCGTGCGTGTGCTACGAGA-3' and the reverse primer used was 5'-GCCGATCGTRAAGAGATGAAGAC3' A FAM-AGTGCAGCCCCGRCCATCGTTC-TAMRA probe was used for detection of amplified product and a standard curve was generated ... 5-TACCTTGCCTGCCTTCCTAC3, R 5-CAACACCAGGCCTTCAAGAC-3); and GAPDH (F 5-GAAGGTGAAGGTCGGAGTC-3, R 5-GAAGATGGT- TLR3 and TLR4 ligands but not TLR2 or TLR9 ligands induce IL-8 secretion in foreskin fibroblasts Initial ... understood although inflammatory changes caused by STI could influence HCMV infection Inflammation in genital tract infections is in many cases caused by the activation of genital tract cells...

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Báo cáo y học: "Type I interferon receptor controls B-cell expression of nucleic acid-sensing Toll-like receptors and autoantibody production in a murine model of lupus" doc

Danh mục: Báo cáo khoa học

... USA) PJU was a member of the scientific advisory boards of Monogram Biosciences, Inc (South San Francisco, CA, USA) and XDx, Inc (Brisbane, CA, USA) and is a cofounder of and consultant to Bayhill ... autoantibodies directed against nucleic acid-associated targets Materials and methods Mice and treatment BALB/cJ mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA) IFNAR2-/- ... was required for the production of IgG autoantibodies directed against the RNAassociated targets, Sm/RNP and RiboP, as well as against the DNA-associated targets, ssDNA and histones Despite failing...

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Báo cáo y học: " Nontypeable Haemophilus influenzae induces COX-2 and PGE2 expression in lung epithelial cells via activation of p38 MAPK and NF-kappa B" pps

Danh mục: Báo cáo khoa học

... impact of p38 MAPK on the activation of NF- kappa B EMSA results showed that the NF- kappa B activation was not prevented by SB203580 in infected A5 49 cells, indicating that p38 MAPK and NFkappa B ... pathway EMSA analysis showed that NFkappa B translocation and its DNA binding activity were markedly increased by NTHi within 90 mins, and DNA binding activity of NF- kappa B was not affected by ... Activation of NF- kappa B by Nontypeable Haemophilus influenzae is mediated by TLR2-TAK1-dependent NIK-IKK alpha/beta-I kappa B alpha and MKK3/6-p38 MAP kinase signaling pathways in epithelial cells...

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Báo cáo khoa học: Human lactoferrin activates NF-jB through the Toll-like receptor 4 pathway while it interferes with the lipopolysaccharide-stimulated TLR4 signaling potx

Danh mục: Báo cáo khoa học

... LPS-dependent NF- jB activation, while it induced NF- jB activation at a level comparable to that induced by intact hLF (Fig 7C) Similarly, puriﬁed hLF carbohydrate chains that can stimulate NF- jB activation ... T Hiraga, K Ihashi, K Sato, N Komagata, H Majima, Y Namekawa, A Suzuki, A Kashimura, M Yamamuro, M Watanabe, K Ito, Y Murase and T Setoguchi for technical assistance We also thank Dr S Miyamoto ... Signaling Technology (Danvers, MA, USA) The TNF -a Human Biotrak Easy ELISA kit was obtained from GE Healthcare BioSciences KK, Japan Actinase E was obtained from KakenSeiyaku Co., Japan Endo -b- galactosidase...

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Báo cáo khoa học: Phosphorylation of NF-jB proteins by cyclic GMP-dependent kinase A noncanonical pathway to NF-jB activation potx

Danh mục: Báo cáo khoa học

... 5¢-GCGGCGGCCTGCCGACCGG GAGCTCAGT-3¢ for S27 6A and 5¢-AAACGTAAAAG GGCATATGAGACCTTCAAGAGCATC-3¢ for T30 5A (mutations in bold) The accuracy of the mutations was conﬁrmed by DNA sequencing p49 (obtained from ... from the NIH AIDS Reagent Repository) was Flag-tagged at the 5¢-end by PCR using the primers 5¢-CTGCAGCATGGACTACAAGGACGACGA TGACAAGGAGAGTTGCTACAACCCAGGTCTG-3¢ and 5¢-GAGAGTTGCTACAACCCAGGTCTG-3¢ ... suggested that NF- jB may be activated by PKG Here we demonstrate that p49, p50, and p65 are substrates for the kinase and we analyze the mechanisms by which PKG induces NF- jB activation Materials and...

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Báo cáo y học: "Differential Constitutive and Cytokine-Modulated Expression of Human Toll-like Receptors in Primary Neutrophils, Monocytes, and Macrophages" docx

Danh mục: Báo cáo khoa học

... and 3.575 μl DNAase free water The oligonucletoide primers for respective TLRs were CTGCAAGCTGCGGAAGATAAT, TLR2; AGAGTTTCCTGCAATGGATCAAG, TLR4; GGCTTAATCACACCAATGTCACTATAG, TLR5; and TCTGAAGACTTCAGGCCCAACT, ... TLR5; and TCTGAAGACTTCAGGCCCAACT, TLR9 for forward primers, and GCAGCTCTCAGATTTACCCAAAA, TLR2; TTATCTGAAGGTGTTGCACATTCC, TLR4; TTAAGACTTCCTCTTCATCACAACCTT, TLR5; and TGCACGGTCACCAGGTTGT, TLR9 for ... The fluorogenic probes were CCGCTGAGCCTCGTCCATGGG, TLR2; TTCGTTCAACTTCCACCAAGAGCTGCCT, TLR4; TACACACAATATATGTCTGCAGGAGGCCCA, TLR5; and AGCACCCTCAACTTCACCTTGGATCTGTC, TLR A GeneAmp 5700 Sequence...

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Báo cáo y học: "No evidence of major effects in several Toll-like receptor gene polymorphisms in rheumatoid arthritis" pdf

Danh mục: Báo cáo khoa học

... 5'AAACGTATCCAATGAAAAGAAG-3' Present study rs1554973 T/C No Forward, 5'CAAAGGATATGTGAACAATAGG-3' Reverse, 5'AATCCCGTGAGTAGAGAATG-3' Present study TLR6 rs5743810 C/T No Forward, 5'ACTTGGTTCGTGATATGTTCTA-3' ... rs3804099 C/T No Forward, 5'ATCGTCTTCCTGGTTCAAGC-3' Reverse, 5'CAGTTCCAAACATTCCACGG-3' Present study rs4696480 T /A No Forward, 5'CAAATTTAAAAGAGGGCAAGAAA-3' Reverse, 5'CAGTTTATTGTGAGAATGAGTTT-3' Present ... A/ G No Forward, 5'CATCCCCTACTTTCTTCACA-3' Reverse, 5'TCAACTCAGGACCCATAATC-3' Present study rs4986790 A/ G 32%/32.50% Yes: other name Asp299Gly Forward, 5'TCTGGGAGAATTTAGAAATGAA-3' Reverse, 5'AAACGTATCCAATGAAAAGAAG-3'...

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Báo cáo y học: "No evidence of major effects in several Toll-like receptor gene polymorphisms in rheumatoid arthritis" ppt

Danh mục: Báo cáo khoa học

... 5'AAACGTATCCAATGAAAAGAAG-3' Present study rs1554973 T/C No Forward, 5'CAAAGGATATGTGAACAATAGG-3' Reverse, 5'AATCCCGTGAGTAGAGAATG-3' Present study TLR6 rs5743810 C/T No Forward, 5'ACTTGGTTCGTGATATGTTCTA-3' ... rs3804099 C/T No Forward, 5'ATCGTCTTCCTGGTTCAAGC-3' Reverse, 5'CAGTTCCAAACATTCCACGG-3' Present study rs4696480 T /A No Forward, 5'CAAATTTAAAAGAGGGCAAGAAA-3' Reverse, 5'CAGTTTATTGTGAGAATGAGTTT-3' Present ... A/ G No Forward, 5'CATCCCCTACTTTCTTCACA-3' Reverse, 5'TCAACTCAGGACCCATAATC-3' Present study rs4986790 A/ G 32%/32.50% Yes: other name Asp299Gly Forward, 5'TCTGGGAGAATTTAGAAATGAA-3' Reverse, 5'AAACGTATCCAATGAAAAGAAG-3'...

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Báo cáo y học: "The role of toll-like receptors in acute and chronic lung inflammation" pdf

Danh mục: Báo cáo khoa học

... Cowan MJ, Hasday JD, Vogel SN, Medvedev AE: Tobacco smoking inhibits expression of proinflammatory cytokines and activation of IL-1Rassociated kinase, p38, and NF- kappaB in alveolar macrophages ... 1(5):398-401 215 Haeberle HA, Takizawa R, Casola A, Brasier AR, Dieterich HJ, Van Rooijen N, Gatalica Z, Garofalo RP: Respiratory syncytial virus-induced activation of nuclear factor-kappaB in the lung ... MoralesSuarez-Varela M, Nilsson L, Braback L, Saraclar Y, Weiland SK, Cookson WO, Strachan D, Moffatt MF: A multi-centre study of candidate genes for wheeze and allergy: the International Study of Asthma and Allergies...

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Báo cáo y học: "Early Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response" ppt

Danh mục: Báo cáo khoa học

... 5'-CCCGGTGTGGCCATTGCTGC-3' 5'-GCACTTTTATCAATTGGCTTAATCAC-3' 5'-AACGAGTCAGGGTACACACAATATATG-3' 5'-CAATGTCACTATAGCTGGGCCTCCTGCAG-3' 5'-CAGTGCTCTTACCCAGATGGA-3' 5'-TCTGATAATCGATGACAGACTTCA-3' 5'-CTGCCTGTGTTTCAATTCACGAAGCT-3' ... 5'-TGAACTGGACTTCTCCCATTTCCGTCTTTT-3' 5'-CCTGGTTTGTTAATTGGATTAACGA-3' 5'-GAGGTGGAGTGTTGCAAAGGTAGT-3' 5'-CCCATACCAACATCCCTGAGCTGTCAA-3' 5'-AGCTCTGCCTTCACTACAGAGACTT-3' 5'-GCTTTTATGGAAACCTTCATGGA-3' ... (TP) TRAM (FP) TRAM (RP) TRAM (TP) Sequence 5'-CCCATTCCGCAGTACTCCATT-3' 5'-TTTCCTTGGGCCATTCCA-3' 5'-CAGTTATCACAAGCTCAAAAGTCTCATGGCCA-3' 5'-TGTGAAGAGTGAGTGGTGCAAGT-3' 5'-ATGGCAGCATCATTGTTCTCAT-3'...

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Báo cáo y học: " Differential expression of Toll-like receptors on human alveolar macrophages and autologous peripheral monocytes" pdf

Danh mục: Báo cáo khoa học

... heat-inactivated pooled human AB serum (Gemini Bioproducts, Sacramento, CA) at 37°C in 5% CO2 Preparation of bronchoalveolar cells Bronchoalveolar cells were obtained by bronchoalveolar lavage ... mRNA upregulation was detected after h and then maintained until 24 h (Figure 5A) LPS upregulated TLR4 mRNA in both monocytes and alveolar macrophages after h only, and was decreased below basal ... following final concentrations per mL: ng Pam3Cys, 100 ng of LPS, μg of mycobacterial DNA (M.tb DNA), and μg of human DNA (control DNA) Culture medium alone was used as a negative control TNF -a and IL-6...

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Báo cáo y học: "Trapped in a vicious loop: Toll-like receptors sustain the spontaneous cytokine production by rheumatoid synoviu" doc

Danh mục: Báo cáo khoa học

... Spontaneous production of proinﬂammatory cytokines and matrix metalloproteinases by RA synovial membrane cells can also be inhibited by overexpression of the dominant-negative form of MyD88 adaptor-like ... Sacre SM, Andreakos E, Kiriakidis S, Amjadi P, Lundberg A, Giddins G, Feldmann M, Brennan F, Foxwell BM: The Toll-like receptor adaptor proteins MyD88 and Mal/TIRAP contribute to the inflammatory ... comparison with IL-1 and TNF blockade Paper presented at: 73rd Annual Scientific Meeting of the American College of Rheumatology/Association of Rheumatology Health Professionals; 20 October 2009;...

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Báo cáo y học: " Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis" ppsx

Danh mục: Báo cáo khoa học

... Recognition of PAMPs by TLRs triggers a signaling pathway that leads to activation of nuclear factor B (NF- B) transcription factors and members of the MAP kinase family [19,20] This activation does ... TLR7 and TLR9 was performed to identify the cellular location of the receptors Ab against TLR4 was evaluated but found not to function in a satisfactory way, and no Ab against TLR10 was available ... rabbit pAbs against TLR2 and TLR3 from AMS Biotechnology Subsequently, HRP-labeled goat anti-mouse or goat anti-rabbit polymer was incubated with the sections for 30 min, followed by 3,3'-diaminobenzidine...

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Báo cáo y học: " Science review: Key inflammatory and stress pathways in critical illness – the central role of the Toll-like receptors" ppsx

Danh mục: Báo cáo khoa học

... of double-stranded RNA and activation of NF- kappaB by Tolllike receptor Nature 2001, 413:732-738 40 Adachi O, Kawai T, Takeda K, Matsumoto M, Tsutsui H, Sakagami M, Nakanishi K, Akira S: Targeted ... forbid signaling in a relatively broad way (Hoebe K et al., in preparation) Not only infectious diseases but perhaps autoimmune diseases also may be approached by blockade of TLR pathways Autoimmune ... complement cascade) [2–5] Activation of complement can lead to destruction of bacteria and to release of bacterial molecules that can activate cell-associated components of the system for microbial sensing...

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The role of downstream of kinase (DOK) 3 in toll like receptor signalling

Danh mục: Y - Dược

... activate TBK1 to phosphorylate IRF3, in a manner similar to TRAF6 assembly of TAB1 and TAB2 that subsequently activate TAK1 for NF B signalling (Kanayama et al., 2004) The mechanism of TRAF3 activation ... of inflammatory-related genes mainly mediated by a niche subset of transcription factors including NF B, AP-1 and IRFs (Sandor and Buc, 2005) 1.4.1 Nuclear Factor B The NF B transcription factor ... TLR3 also interacts with PI3K and activates AKT, leading to further phosphorylation and maximal activation of IRF3 (Sandor and Buc, 2005) 1.2.6 Positive and negative regulators of TLR signalling...

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báo cáo hóa học: " Toll-like receptors in cerebral ischemic inflammatory injury" pdf

Danh mục: Toán học

... synthesis, activation of the proinflammatory transcription factor NF- B, and induction of inflammatory cytokines such as TNF -a, IL- 1b, and IL-6 have been demonstrated [46] Suppression of the normal inflammatory ... location of the complex, the phosphorylated IRAKs are able to bind TRAF3 in addition to TRAF6 Activation of TRAF3 leads to phosphorylation, dimerization, and nuclear localization of the transcription ... regulates TLR-mediated NFkappa B activation by targeting TAB2 and TAB3 for degradation Nat Immunol 2008, 9:369-377 58 Dalpke AH, Lehner MD, Hartung T, Heeg K: Differential effects of CpGDNA in...

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Báo cáo y học: "Toll-like receptors and innate immune responses in systemic lupus" docx

Danh mục: Báo cáo khoa học

... containing DNA selectively activates TLR9 CpG motifs in mammalian DNA are relatively rare and generally methylated, so that mammalian DNA is a weak TLR9 agonist [12] In addition, mammalian DNA ... nucleic acids, and ultimately for the production of autoantibodies to nucleic acids and proteins bound to nucleic acids Disruption of this signaling may be a major mechanism of action of antimalarial ... lupus pathogenesis is unknown and other inflammatory cytokines stimulated by TLR activation, such as TNF-α and IL-6, may also play key roles Cellular debris and immune clearance A likely source of...

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Báo cáo y học: "Toll-like receptors and NOD-like receptors in rheumatic diseases" pdf

Danh mục: Báo cáo khoa học

... open-label study of Anakinra in 10 patients with gout that could not tolerate or had failed standard antiinflammatory therapies All patients received Anakinra daily for days and all showed rapid ... for the inflammasome Nat Immunol 2009, 10:266-272 Kawane K, Ohtani M, Miwa K, Kizawa T, Kanbara Y, Yoshioka Y, Yoshikawa H, Nagata S: Chronic polyarthritis caused by mammalian DNA that escapes from ... Hill AV: A Mal functional variant is associated with protection against invasive pneumococcal disease, bacteremia, malaria and tuberculosis Nat Genet 2007, 39:523-528 Tian J, Avalos AM, Mao SY,...

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Báo cáo y học: " Gut flora enhance bacterial clearance in lung through toll-like receptors 4" docx

Danh mục: Báo cáo khoa học

... sequences are 5’ CGTCTAGACTTTCTCCGTTACTTGG3’ (antisense) for KC, 5’ GAACAAAGGCAAGGCTAACTGA3’ (sense) and 5’ AACATAACAACATCTGGGCAAT3’ (antisense) for MIP-2 5’ CAGCACGAGGCTTTT TTGTTG3’ (sense) and 5’ ... LPS on pulmonary NF- B activation and bacterial killing activity of alveolar macrophages in TLR4 mutant mice further corroborates that gut flora are important in enhancing NF- B activation in the ... Company) Bacterial killing activity of alveolar macrophages Alveolar macrophages (AM) were harvested from adult C3H/HeN and C3He/HeJ mice by bronchoalveolar lavage (BAL) with Tris-buffered saline...

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Báo cáo y học: " Bench-to-bedside review: Toll-like receptors and their role in septic shock" pps

Danh mục: Báo cáo khoa học

... a complex of MyD88 (an adapter protein) and IRAK (a kinase) IRAK then phosphorylates TRAF6, which leads to NF- B- inducing kinase and I- B kinase activation The I- B kinase phosphorylates I- B, ... lipoteichoic acid from Gram-positive bacteria and live Mycobacteria tuberculosis bacteria (Table 2) Purified glycolipids from Treponema brennaborense, a spirochete that causes a bovine infectious disease, ... 2:346-352 44 Takeuchi O, Hoshino K, Kawai T, Sanjo H, Takada H, Ogawa T, Takeda K, Akira S: Differential roles of TLR2 and TLR4 in recognition of Gram-negative and Gram-positive bacterial cell wall components...

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