10 methylquant a real time pcr based method to quantify dna methylation at single specific cytosines
Báo cáo hóa học: "Research Article Experimental Investigation of Cooperative Schemes on a Real-Time DSP-Based " docx
... for channel estimation are a ected by the noise at the relay, and of the back-off Thus we can not estimate the relay -to- destination propagation channel at the destination For this reason we have ... though), and therefore we can calculate the BER also based on this data When we calculate the duty cycle we assume that these symbols were actually carrying payload data The results should be the same ... should be the same as in a case where synchronisation had occurred in a dedicated synchronisation phase The air interface employed for payload data is the same as for AF and DF, that is, 48 symbols,...
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Báo cáo hóa học: " A Real-Time Model-Based Human Motion Tracking and Analysis for Human Computer Interface Systems" docx
... A Real- Time Model -Based Human Motion Tracking and Analysis It can only track the restricted movement of walking human parallel to the image plane Another real time system, Pfinder [11], starts ... integrator, and one animator Each viewer estimates the partial BDPs from the extracted foreground image and sends the results to the BDP integrator The BDP integrator creates a universal 3D model ... Update partial BDP BAP integrator 1D position identification BDP perspective scaling Human body 2D position estimation 1D position identification BDP perspective scaling Facade/flank arbitrator BAP...
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Báo cáo hóa học: " A quantitative real time PCR method to analyze T cell receptor Vb subgroup expansion by staphylococcal superantigens" doc
... cgcacatatggatgtcggagttttgaat gcgcggatcctcaactttcgtccttata SElN AF285760 aatgctcatatggacaaaaaagatttaaag gcgcggatccttaatctttatataaaa SElO AF285760 tgcactcgagaatgaagaagatcctaaa cgcgctcgagttatgtaaataaataaac Seo et al ... 3’) SEA SED M18970 M28521 cttgtacatatgagcgagaaaagcgaagaa cgttctcgagaatgaaaacattgattc gcgcggatccttaacttgtatataaata cgcgctcgagctacttttcatataaata SEE M21319 ggtagccatatgagcgaagaaataaatgaa gcgcggatcctcaagttgtgtataaata ... gcgcggatcctcaagttgtgtataaata SEG AF064773 tgtgcatatgcaacccgatcctaaatta gcgcggatcctcagtgagtattaaga SEI AF285760 tgctctcgaggatattggtgtaggtaac cgcgctcgagttagttactatctacata SElM AF285760 cgcacatatggatgtcggagttttgaat...
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Báo cáo y học: " Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCR" doc
... conventional PCR and real- time PCR for unknown samples Conventional PCR Real time PCR + Subtotal - + 29 29 - Total +, positive; -, negative 37 Abbreviations bp: base pair; cDNA: complementary DNA; LOD: ... plasmid copy number LOD and LOQ of the assay TaqMan real- time PCR Real- time PCR was carried out on an ABI 7500 thermocycler (Applied Biosystems, CA, USA) with a final volume of 25 μL The real- time ... Primer-1 5’-CGGATATTGTAKTCCTGGTCGTA-3’ Primer-2 5’-CCTGTCCTAGATTCCCCTATTGATT-3’ Probe FAM-5’-CTAGGCCTACGTGGTCTACATTTC-3’-TAMRA Three PCV2-positive samples and 37 serum and tissue unknown samples were...
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Báo cáo y học: " Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene" doc
... likely to produce a false positive than a conventional PCR assay or the SYBR Green I -based real- time PCR TaqMan -based real- time PCR may be more specific than SYBR Green I -based real- time PCR because ... clinical samples by real- time PCR vs conventional PCR Conventional PCR Positive Negative 1 1a 0b 21c 9d Real- time PCR Positive Negative a, d indicate the number of samples that generated similar data ... primers and probe were: NS1-FP (forward primer): 5’-GAAGACTGGATGATGACAGATCCA-3’, NS1-RP (reverse primer): 5’-TGCTGTTTTTGTTCT TGCTAGAGTAA-3’ NS1-P (probe): FAM-AATGATGGCTCAAACCGGAGGAGA-BHQ1 The...
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Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx
... attachment to CAR, a 46-kDa transmembrane protein that also serves as a receptor for many adenoviruses [17] In addition, some strains of CVB1, and can interact with an additional receptor, DAF, ... viral attachment capacity In this article, we demonstrate that RT -PCR is an alternative, rapid and efficient method to study viral interaction with the cell surface RTPCR can complement or replace ... be due to the fact that DAF is ten times more abundant than CAR on HeLa cells [38], and that CVB2O previously has been shown to have a significantly slower attachment rate to cells than CVB5...
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Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR
... D., Sasaki Y., Maruyama T., Yanagisawa E., Hiraishi A and Kato K (2001) Degradation of the cyanobacterial hepatotoxin microcystin by a new bacterium isolated from a hypertrophic lake Environ Toxicol., ... blue-green algae, Microcystis, Anabaena, Oscillatoria and in lake Kasumigaura Environ.Tech., 14, 433-442 Park H.-D., Iwami C., Watanabe M F., Harada K.-I., Okino T and Hayashi H (1998) Temporal variabilities ... Table - Primers and TaqMan Probes used Primer and Probe Sequence (5’-3’) Reference MF MR gacccgatgttcaagatact ctcctcccacaaatcaggac Saito et al., 2003b QMF QMR QMT (Probe) agacgcacgctcacctcaa...
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Báo cáo sinh học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" pdf
... TGC TYG A TTC CCC TGG AGA AGT ACT CCT CGA ACT AAA TCC ATA CCT AGC ACAC CAG TGG CGG GAC AAA CCA AC CTC TCC TGG AGA ACT CCT ACT TGA T GAA GTC TTG CCT TTA GAG CCC GTC CAG TTG CGG GAG CTT CAA TCG CT ... AAT GGA TCC TGT RGC RG GAA CCC GTG GCY GGG CCA AC CCA GAG GCA AAY ACA GCT GAG TGA CCC TGT GGC TGG CTC CTC ATG CTG AAC CAC TGC CWC TTG AT CAA ATT TCA ATG GAY CCT GTT GCG GGT AGT GGG CGC CGC AAC ... Sequence Reference COG1F G1NVR CGY TGG ATG CGN TTY CAT GA ACC CAR CCA TTA TAC ATY TG [7] COG2F CAR GAR BCN ATG TTY AGR TGG ATG AG ACC NGC ATA NCC RTT RTA CAT TC A [7] G2NVR B Probes GI/1 GI/2 GI/3...
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Báo cáo sinh học: " Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" potx
... viral culture and PCR assays are largely negative [15] Several commercially available HSV-type specific serological assays are available, but a test such as a HSV-2 Western blot is expensive and ... directed against λ DNA The performance of the PCR was monitored by quantitative real- time PCR (qPCR) The mean cycle threshold (Ct) and the standard deviation of the controls were calculated Samples ... by EANA, MJH and AN; interpretation and laboratory work was conducted by MJH, EANA, AN, SK and RB; EANA, RLB, SK and MJH were responsible for analysis of results and preparation of the manuscript...
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Báo cáo hóa học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" ppt
... TGC TYG A TTC CCC TGG AGA AGT ACT CCT CGA ACT AAA TCC ATA CCT AGC ACAC CAG TGG CGG GAC AAA CCA AC CTC TCC TGG AGA ACT CCT ACT TGA T GAA GTC TTG CCT TTA GAG CCC GTC CAG TTG CGG GAG CTT CAA TCG CT ... AAT GGA TCC TGT RGC RG GAA CCC GTG GCY GGG CCA AC CCA GAG GCA AAY ACA GCT GAG TGA CCC TGT GGC TGG CTC CTC ATG CTG AAC CAC TGC CWC TTG AT CAA ATT TCA ATG GAY CCT GTT GCG GGT AGT GGG CGC CGC AAC ... Sequence Reference COG1F G1NVR CGY TGG ATG CGN TTY CAT GA ACC CAR CCA TTA TAC ATY TG [7] COG2F CAR GAR BCN ATG TTY AGR TGG ATG AG ACC NGC ATA NCC RTT RTA CAT TC A [7] G2NVR B Probes GI/1 GI/2 GI/3...
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báo cáo hóa học:" Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" pdf
... viral culture and PCR assays are largely negative [15] Several commercially available HSV-type specific serological assays are available, but a test such as a HSV-2 Western blot is expensive and ... directed against λ DNA The performance of the PCR was monitored by quantitative real- time PCR (qPCR) The mean cycle threshold (Ct) and the standard deviation of the controls were calculated Samples ... by EANA, MJH and AN; interpretation and laboratory work was conducted by MJH, EANA, AN, SK and RB; EANA, RLB, SK and MJH were responsible for analysis of results and preparation of the manuscript...
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Luận văn : BƯỚC ĐẦU XÂY DỰNG QUY TRÌNH ĐỊNH LƯỢNG CÁC SẢN PHẨM BIẾN ĐỔI GEN BẰNG PHƯƠNG PHÁP REAL-TIME PCR part 10 ppsx
... chiếu: PCR Standard Curve Report with PCR Amp Cycle Graph PCR Base Line Subtracted Data Current Date: Data generated on: 19-Aug-05 12:22 PM 16-Aug-05 at 04:58 PM Optical data file name: Plate Setup ... 35S: PCR Standard Curve Report with PCR Amp Cycle Graph PCR Base Line Subtracted Curve Fit Data Current Date: Data generated on: 19-Aug-05 12:17 PM 16-Aug-05 at 04:58 PM Optical data file name: ... 35S: PCR Standard Curve Report with PCR Amp Cycle Graph PCR Base Line Subtracted Curve Fit Data Current Date: Data generated on: 11-Aug-05 05:04 PM 03-Aug-05 at 09:21 PM Optical data file name:...
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Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps
... aggccttcgggttgtaaagt gttagccggtgcttcttctg FAM-aaccgcagcaattgacgttaccc-BHQ 1a tgcagaaaattgatgctgct ttgcccaggttggtaatagc JOE-acctgggtgcggtacagaaccgt-BHQ 1a ggtaaaggggcttcggtatc tattggctccctgaatacgc Cy5-tggtggtgtagccactgtcccgt-BHQ 1a ... Although the multiplex real- time PCR assay was demonstrated as an applicable assay in artificially inoculated meats, it needs further research for natural meat cases and other types of food and ... distilled water (Gibco, USA) was then added to adjust to a final volume of 200 μl An aliquot of μl of the supernatant was used as the template DNA in the multiplex real- time PCR iii) QIAamp DNA Mini...
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Báo cáo y học: " “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies.”" pptx
... extracted DNA samples were used for quantitative PCR assays 10 Real Time PCR Assay Real- time PCR targeting the morphologically transforming region mtr II sequence was applied onto the DNA extracted ... Christmas SE, Halliday D, Lawton N, Wang H, Abdalla I, Masters J, Hassan RL, Hart IJ, Khan N, Smith J, Hammad A, Bakran A: Cytomegalovirus -specific CD8+ T cells not develop in all renal transplant ... renal transplant patients was higher (20-60%) compared to western literature [14] A study carried out in southern part of India among renal transplant patients by application of the quantitative...
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Báo cáo y học: " Development of a new ultra sensitive real-time PCR assay (ultra sensitive RTQ-PCR) for the quantification of HBV-DNA" pot
... 5’-GCCAAAATTCGCAGTCCC-3’, 0.5 μM primer hbv460 (antisense) 5’-GATAGTCCAGAAGAACCAACAAGAAG-3’, 0.4 μM molecular beacon 5’-CG CGCGATGAGGCATAGCAGCAGGATGAAGAACG CGCG-3’ labelled with FAM and Dabcyl at ... RTQ -PCR against the WHO 1st International Standard for HBV -DNA The calibration of the in-house assay was done against the WHO st International Standard for HBV -DNA (OptiQuant® HBV -DNA Quantification ... The calibration of the HBV -DNA values was performed against the WHO 1st International Standard for HBVDNA (OptiQuant® HBV -DNA Quantification Panel, Accrometrix Europe B.V.) Specifically, serial...
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Báo cáo khoa học: " Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection" pps
... detection and quantification The additional advantage of utilizing SYBR Greenbased real- time RT -PCR is that it is relatively easy to design and test primer pairs that are suitable for RT -PCR analysis ... quantitative measurement, lower contamination rate, higher sensitivity, higher specificity, and easy standardization Thus, quantitative RT -PCR assay might eventually replace virus isolation and ... Level facilities Supernatants were obtained days after virus inoculation and stored at -80°C Viral RNA was extracted (Qiamp viral RNA kit; Qiagen, Germany) from the virus supernatant, titrated...
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Báo cáo khoa học: " A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes" pps
... 5’-TTGGTTACCATGCAAACAAYT-3’ 91-111 Antisense 5’-TRTCTTGGGCRTGTGTAACA-3 152-171 Probe 119-143 5’-FAM-CAGGTTGACACAATAATGGAAAAGBHQ3-3’ a Y = T or C, R = A or G LNA residues in the probe are indicated ... assay failed to detect virus in a nasal swab and a throat swab (Table 1) This may be due to RNA degradation during long-term storage and multiple freezethaw cycles Tran Tan et al Virology Journal ... influenza virus in Asia: implications for pandemic control Proc Natl Acad Sci USA 2006, 103(8):2845-2850 Li KS, Guan Y, Wang J, Smith GJ, Xu KM, Duan L, Rahardjo AP, Puthavathana P, Buranathai C,...
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Báo cáo khoa học: "Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA" ppt
... CCGGCCCCTGAATGC-3' Genbank: NC_002058.3 (447–461) Reverse primer 5'-ATTGTCACCATAAGCAGCCA-3' Genbank: NC_002058.3 (596–577) 5' CACCGGATGGCCAATCCA-3' Genbank: NC_002058.3 (639–622) Probe FAM 5' AACCGACTACTTTGGGTGTCCGTGTTTC-3' ... AACCGACTACTTTGGGTGTCCGTGTTTC-3' TAMRA Genbank: NC_002058.3 (535–562) ml From each concentration replicates were extracted with both extraction methods and each RNA extract was analysed on both real- time PCR instruments Additionally, ... Poelstra E, Hooghiemstra M, Brandenburg AH: Clinical validation of a new real- time PCR assay for detection of enteroviruses and parechoviruses, and implications for diagnostic procedures Journal...
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Báo cáo khoa học: "Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA" doc
... CCGGCCCCTGAATGC-3' Genbank: NC_002058.3 (447–461) Reverse primer 5'-ATTGTCACCATAAGCAGCCA-3' Genbank: NC_002058.3 (596–577) 5' CACCGGATGGCCAATCCA-3' Genbank: NC_002058.3 (639–622) Probe FAM 5' AACCGACTACTTTGGGTGTCCGTGTTTC-3' ... AACCGACTACTTTGGGTGTCCGTGTTTC-3' TAMRA Genbank: NC_002058.3 (535–562) ml From each concentration replicates were extracted with both extraction methods and each RNA extract was analysed on both real- time PCR instruments Additionally, ... Poelstra E, Hooghiemstra M, Brandenburg AH: Clinical validation of a new real- time PCR assay for detection of enteroviruses and parechoviruses, and implications for diagnostic procedures Journal...
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