1 specimen setup for isolation and identification
Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... concentration of G 418 (Invitrogen) of 400 lgÆmL )1 G 418 -resistant colonies were picked and expanded for PCR genotyping and the formation of embryoid bodies One ESC clone (T26) out of 48 G 418 -resistant ... trypsinized, collected and counted Cells (1 · 10 7) were resuspended with 50 lL of MACS buffer containing 50 lgÆmL )1 antibody against Lyve -1 (MAB 212 5, R&D) and incubated on ice for 5 10 The primary antibody-labeled ... vascular pattern and increased vascular fragility J Cell Biol 16 2, 11 11 11 22 Lindblom P, Gerhardt H, Liebner S, Abramsson A, Enge M, Hellstrom M, Backstrom G, Fredriksson S, Landegren U, Nystrom...
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Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
... P 010 726-26; lane 8, E 010 206 -13 -2; lane 9, J 010 303 -11 -1; lane 10 , O157-R1-3-2; lane 11 , O157-C -1- 2; lane 12 , A1; lane 13 , A2; lane 14 , A3; lane 15 , A4 lanes 16 - 21, strains C1, C2, C3, C4, C5, and ... 5, P 010 726-24; lane 6, P 010 726-25; lane 7, P 010 726-26; lane 8, E 010 206 -13 -2; lane 9, J 010 303 -11 -1; lane 10 , O157-R1-3-2; lane 11 , O157-C -1- 2; lane 12 , ATCC 43894 (a positive control); M, 10 0 bp ... P 010 726- 21 and P 010 726-24 Subgroup E2 was broken down by Korean isolates (P 010 726 -18 , P 010 726-22, P 010 726-23, P 010 726-25, and O157-C -1- 2), and 14 USA isolates; strains of ATCC (A1, A2, A3, and...
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Báo cáo khoa học: " Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)" ppt
... to 10 0% identical to the other strains of CCV, including CCV1- 71 (AF 116 246), CCV6 (A22882), CCV C54 (A22886), CCV INSAVC (D13096), UWSMN- (AF327928), CCV TN449 (AF 116 245), and CCV58 21 (AB 017 789) ... 262 Feng-Shan Gao et al 10 volumes of Hank’s solution containing 10 % FCS, followed by centrifugation at 3,000 rpm for 10 to remove large cell debris and at 8,000 rpm for 10 to remove other particles ... characterization of a new Australian isolate J Clin Microbiol 20 01, 39, 10 36 -10 41 Nermut MV Negative staining of viruses J Microsc 19 72, 96, 3 51- 362 Roche RR, Alvarez M, Guzman MG, Morier L, Kouri G Comparison...
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Báo cáo y học: "Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)" potx
... properties [17 ], namely andrographolide, 14 -deoxy -11 ,12 -didehydroandrographolide, ergosterol peroxide, 14 -deoxy -14 ,15 -dehydroan- drographolide, 5-hydroxy-7,8-dimethoxyflavone, 19 -Oacetyl -14 -deoxy -11 ,12 -didehydroandrographolide, ... (i.p 10 0 mg/kg, 3d) [34,35, 91] 14 -deoxy -11 ,12 -didehydroandrographolide muscle relexation NO release from endothelial cells [10 7 ,10 5] 14 -deoxy -14 ,15 -didehydroandrographolide cytotoxic activity and ... aggregation in vitro [10 7] Andrographolide and 14 -deoxy -11 ,12 -didehydroandrographolide Chao and Lin Chinese Medicine 2 010 , 5 :17 http://www.cmjournal.org/content/5 /1/ 17 Page of 15 $QGURJUDSKLV SDQLFXODWD...
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Isolation and identification of components from ixeris sonchifolia hance as potential anti stroke agents
... Introduction 1. 1 Stroke 1. 1 .1 Pathophysiology 1. 1.2 Epidemiology 1. 1.3 Progress in stroke treatment 10 1. 1.3 .1 Thrombolysis 10 1. 1.3.2 Anticoagulants and antiplatelet drugs 11 1. 1.3.3 Neuroprotectants 13 ... structures of compounds 11 to 13 71 3.3 .1. 5 Chemical structures of compounds 14 to 18 71 3.3.2 .1 Representative HPLC profiles for purified compounds to and and fractions to 74 3.4 .1 Structures of sesquiterpene ... BV-2 cells 11 1 5.3.5 .1 Effects of flavonoid compounds to on the expression of iNOS in LPS-stimulated BV-2 cells 11 2 5.4 .1 The inflammatory cascade following a stroke event 11 5 6.2.2 .1 Schematic...
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English for Tourism and Hospitality 1
... Certainly Certainly Just a minute please Answers: 1) Good morning, Plaza Hotel Leo speaking 2) Good morning, Plaza Hotel Leo speaking How can I help you? 1) Just a minute please 2) Would you mind holding ... _ mind you would please holding? I while you on hold put may I find you out for? _ you put I can hold on? ... EXERCISES Key vocabulary Look up the meaning and pronunciation of these words in your dictionary reservation book cost single certainly per night...
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English for Tourism and Hospitality 1
... N for November O for Oscar P for Papa Q for Quebec R for Romeo S for Sierra T for Tango U for Uniform V for Victor W for Whisky X for X-ray Y for Yankee Z for Zulu A for Alfa B for Bravo C for ... Zulu A for Alfa B for Bravo C for Charlie D for delta E for Echo F for Foxtrot G for Golf H for Hotel I for India J hor Juliet K for Kilo L for Lima M for Mike Bây mời bạn nghe lại đối thoại lần ... book two rooms for myself and my father Could you tell me the cost of a single room per night? Leo: Certainly A single room is $12 0 American dollars per night Mona: Fine Leo: And when would you...
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Automation of Call Setup in IP Telephony for Tests and Measurements
... Survived Call Setup Time (sec) Delay Vector (sec) 04 08 12 15 18 19 24 3.9 to 4.70 3.4 to 10 .2 3.5 to 10 .2 3.5 to 10 .2 3.5 to 10 .2 3.5 to 10 .2 3.5 to 10 .2 {0, 0, 0, 0, 0, 0} {0, 1, 2, 3, 4, 5} ... 5} {0, 2, 4, 6, 8, 10 } { 0, 3, 6, 9, 12 , 15 } {0, 4, 8, 12 , 16 , 20} {0, 5, 10 , 15 , 20, 25} {0, 6, 12 , 18 , 24, 30} REFERENCES Website of Hammer Technologies, www.hammer.com, 19 99 (or http://www.empirix ... INTRODUCTION 15 3 Figure B -1 A simple testbed for measuring the call setup performance of IP-PSTN GWs E, Ethernet link; T1, CAS or PRI link; BRI, basic rate interface links NTS stands for network...
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Tài liệu A resource for reading and words part 1 ppt
... have to find a way to our fifty thousand members as an educational and propaganda machine Music, obviously, can make a mood, build familiarity and memory, and for an happy event He has always been ... www.elt-time.com/forum PASSAGE THE BRITISH MENTALITY Although I had stayed in England for over a year, it was difficult for me to understand the British mentality Traveling ... Elvis' friends and the relatives surprised the writer PASSAGE , WORDS Because words can break silence, and thus remove tension, we often draw on them for this reason and not mainly for what they...
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Tài liệu Báo cáo khoa học: Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf
... RJ (20 01) MKK7 is an essential component of the JNK signal transduction pathway activated by proinflammatory cytokines Genes Dev 15 , 14 19 14 26, doi :10 .11 01/ gad.8885 01 Ip YT & Davis RJ (19 98) Signal ... mitogen-activated protein kinase signaling network Science 297, 10 18 10 23, doi :10 .11 26/science .10 68873297 ⁄ 5583 ⁄ 10 18 [pii] 11 Hornberg JJ, Bruggeman FJ, Binder B, Geest CR, de VaateAJ, Lankelma ... 3203–3 213 , doi :12 10 412 [pii] 10 .10 38/sj.onc .12 10 412 20 Shen Y, Luche R, Wei B, Gordon ML, Diltz CD & Tonks NK (20 01) Activation of the Jnk signaling pathway by a dual-specificity phosphatase, JSP-1...
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Recommendations for Use of Antiretroviral Drugs in Pregnant HIV-1-Infected Women for Maternal Health and Interventions to Reduce Perinatal HIV Transmission in the United States pdf
... of HIV -1: NVAZ randomised clinical trial Lancet Oct 11 2003;362(93 91) :11 71- 117 7 Available at http://www.ncbi.nlm.nih.gov/pubmed /14 568737 Taha TE, Kumwenda NI, Hoover DR, et al Nevirapine and zidovudine ... Infect Dis Mar 2 011 ;11 (3) :17 1 -18 0 Available at http://www.ncbi.nlm.nih.gov/pubmed/ 212 37 718 17 Wade NA, Birkhead GS, Warren BL, et al Abbreviated regimens of zidovudine prophylaxis and perinatal ... male and female genital tract Antivir Ther 2 011 ;16 (8) :11 49 -11 67 Available at http://www.ncbi.nlm.nih.gov/pubmed/2 215 5899 Recommendations for Use of Antiretroviral Drugs in Pregnant HIV -1- Infected...
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Báo cáo khóa học: ICAM-1 expression is highly NF-jB-dependent in A549 cells No role for ERK and p38 MAPK docx
... stimulated with TNFa (10 ngÆmL )1) or IL-1b (1 ngÆmL )1) and harvested at 0, 2, and 18 h post stimulation, prior to Western blot analysis of ICAM -1 A representative blot is shown and optical densities ... concentrations of PD098059, SB203580 or U 012 6 (0. 01 10 0 lM) before stimulation with either TNFa (10 ngÆmL )1) or IL-1b (1 ngÆmL )1) Cells were harvested after h for luciferase activity determination ... Pharmacol 13 1, 99 10 7 15 Mills, P.R., Davies, R.J & Devalia, J.L (19 99) Airway epithelial cells, cytokines, and pollutants Am J Respir Crit Care Med 16 0, S38–S43 16 Papi, A (19 97) Epithelial ICAM -1 regulation...
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Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx
... to 15 lm for P50H, H50P, P128H, H128P, PigE17DCPT1B and HumanD17ECPT1B, and from to 500 lm for D18PigCPT1B and D28PigCPT1B) The assay was performed at mm carnitine as standard To analyze PigE17DCPT1B ... (nmolÆmin )1 mg )1) Strain Wild-type Pig CPT1B Human CPT1B Deletion and chimeras D18PigCPT1B D28PigCPT1B H50P H128P P128H P50H a P < 0.0 01, b 2.79 ± 1. 90 4.43 ± 2.98 15 .28 9.42 9.43 10 .59 4 .16 4.49 ... (GAPDH) gene promoter (GAPp), in the pHW 010 plasmid [6,32], to produce P50H–pHW 010 , P128H–pHW 010 , H50P–pHW 010 , H128P–pHW 010 , PigE17D–pHW 010 , and HumanD17E–pHW 010 These constructs were linearized in...
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Báo cáo khoa học: Ki-1⁄57 interacts with PRMT1 and is a substrate for arginine methylation pptx
... In vitro methylation and phosphorylation Yeast two–hybrid screenings and interaction analysis pBTM 116 -Ki -1 ⁄ 57 (12 2– 413 ) [11 ], pBTM 116 -Ki -1 ⁄ 57 (1 15 0) and pBTM 116 -PRMT1 (1 344) vectors were used ... 3948 1 PRMT1 (HRMT1L2 ⁄ ANM1 ⁄ HCP1 ⁄ IR1B4) Tubulin betapolypeptide Ubiquitin-conjugating enzyme E 21 hnRNP-A3 (FBRNP ⁄ D10S102 ⁄ 2 610 510 D13Rik) Daxx (DAP6 ⁄ BING2 ⁄ Fas-binding protein) 15 00 14 00 ... Functional association of Ki -1 ⁄ 57 and PRMT1 13 14 15 16 17 18 19 20 21 22 23 24 D O Passos et al not of p53, is inhibited by the physical interaction with RACK1 and its inhibitory effect is...
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
... 1B88:B, 1D9K:E, 1H5B:B, 1KB5:A, 1KJ2:A (T-cell receptor alpha domains); 1BJM:A, 2RHE (Bence–Jones proteins); 1AY1:H, 1BGX:H, 1IGF:J, 1KEN:T, 2IGF:H (VH domains); 1IAI:M, 1F6L:L, iIFF:L, 1JNL:L and ... undertaken and the final model selection based on the modeller objective score Construction of the 12 F -11 DCys mutant and 12 F -11 reduction/alkylation For reduction and alkylation of 12 F -11 , recombinant ... monomeric proteins 12 F -11 and 12 F -11 DCys (40 lgÆmL )1) to immobilized Tom70 protein The inset shows the binding profile of protein 12 F -11 DCys to immobilized Tom70 and to a nonspecific antigen and a blank...
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Báo cáo khoa học: Colocalization of insulin receptor and insulin receptor substrate-1 to caveolae in primary human adipocytes Cholesterol depletion blocks insulin signalling for metabolic and mitogenic control doc
... caveolin and caveolae in insulin signaling and diabetes Am J Physiol 285, E 115 1–E 116 0 ˚ 16 Stralfors, P (19 97) Insulin second messengers Bioessays 19 , 327–335 17 Gustavsson, J., Parpal, S., Karlsson, ... Cell Biol 11 7, 11 81 11 96 McKeel, D.W & Jarett, L (19 70) Preparation and characterization of a plasma membrane fraction from isolated fat cells J Cell Biol 44, 417 –432 Oka, Y & Czech, M.P (19 84) Photoaffinity ... (19 98) Intracellular localization of phosphatidylinositide 3-kinase and insulin receptor substrate -1 in adipocytes: Potential involvement of a membrane skeleton J Cell Biol 14 0, 12 11 12 25 21...
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Báo cáo khoa học: Differential binding of factor XII and activated factor XII to soluble and immobilized fibronectin – localization of the Hep-1/Fib-1 binding site for activated factor XII pdf
... IgG (1 : 10 0) and rabbit anti-FN IgG (1 : 10 0) for h, and second with a mixture of Alexa 594-conjugated donkey anti-(goat IgG) (1 : 800) and Alexa 488-conjugated goat anti-(mouse IgG) (1 : 800) ... Fibronectin’s III -1 module contains a conformationdependent binding site for the amino-terminal region of fibronectin J Biol Chem 269, 19 183 19 187 10 Oberhauser AF, Badilla-Fernandez C, Carrion-Vazquez ... Biol 18 , 13 63 13 70 FEBS Journal 275 (2008) 516 1– 517 2 ª 2008 The Authors Journal compilation ª 2008 FEBS 517 1 Factor XII binding to fibronectin I Schousboe et al 29 Schwarzbauer JE & Sechler JL (19 99)...
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Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells potx
... Biochem 2 71) 15 61 Fig PAK1 activation depends on Cdc42 and Rac1 (A) A2058 cells were serum-deprived and treated with or without 10 lM LY294002 (LY) for h before incubating with LPA (2 lM) for Cellular ... (10 0 ngÆmL )1, h), LY294002 (10 lM, h), U7 312 2 (10 lM, 0.5 h), PD98059 (25 lM, h), genistein (10 0 lM, h), Y27632 (10 0 lM, h) and further incubated with or without lM LPA for min, and PAK1 activity ... the dominant negative forms (N17Rac1, N17Cdc42 and N19RhoA) and the corresponding control vectors, and the cells were incubated in the absence or presence of lM LPA for PAK1 activity was assessed...
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formal methods for components and objects 9th international symposium, fmco 2010, graz, austria, november 29-december 1, 2010 revised papers
... described for our policy For concreteness, let us consider a state where we have a manager m1 , three employees e1 , e2 and e3 , and two groups g1 = {e1 , e2 } and g2 = {e3 } Consider moreover files f1 ... theoretically in terms of complexity and decidability [ 21, 12 ,15 ], and in terms of methods and tools that are practically feasible automated verification [8 ,1, 13 ,16 ] Limitations of Security Protocol ... ); g1 - > isOwner ( f1 ); g2 - > isOwner ( f2 ); } By our access control rules, e1 and e2 can access f1 and e3 can access f2 When a manager successfully issues the command e1->changeGroup(g1,g2),...
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