... there was a marked increase in the amount of 16 :0 and a decrease in the amount of 16 :1 and 17 :1 in the lipid droplet fraction (Table 2) The fatty acid analyses of fractions taken from blank gradients ... of palmitic, stearic and oleic acids (16 :0, 18 :0, 18 :1, respectively) The DSM fractions contained higher levels of 18 :0, 18 :2 + 18 :3 and 18 :1 at the expense of 16 :0, 14 :0 and 16 :1, whereas there ... with
... the next three years After all, it might have been far worse It might have happened in a campaign year This way he still had a fighting chance Three sessions with a good record might overbalance ... a suit coat from a hanger and left the office with it under his arm A moment later the door opened again and the senator saw the shaggy head of his older son peer into the room The boy was the ... to have evolved a particularly deadly strain of bacteria, which he had been toting around with him in an aspirin bottle," Ambly went on, his thin hands clasped tightly in front of him 14 "Of...
... 0.75 are shown Black geometrical shapes are additional domains, as indicated ANOGA, Anopheles gambiae; ASHGOS, Ashbya gossypii; BRARE, Brachydanio rerio; CANDGLA, Candida glabrata; CIONA, Ciona ... Flodin A & Skalnik DG (2000) Cloning ofa mammalian transcriptional activator that binds unmethylated CpG motifs and shares a CXXC domain with DNA methyltransferase, human trithorax, and methyl-CpG ... glioblastoma multiforme Oncogene 20, 410 7– 411 4 19 Peters AH, O’Carroll D, Scherthan H, Mechtler K, Sauer S, Schofer C, Weipoltshammer K, Pagani M, Lachner M, Kohlmaier A et al (20 01) Loss of the Suv39h...
... Catalytic features of anti-HpU-9 mAb light chain light and ⁄ or heavy chains from mAbs such as i 41 7 [13 ], i41SL1-2 [14 ], and ECL2B [15 ] The light chain of ECL2B mAb was capable of cleaving a ... show any catalytic activity in this analysis, which confirmed the result that had previously been reported [9 ,11 ,13 15 ] The isolated heavy Catalytic features of anti-HpU-9 mAb light chain chain ... the whole antibody However, not all of the isolated heavy or light chains change their specificity In the case of HpU -17 and )20 mAb (a series of mAbs obtained along with HpU-9) [17 ], their heavy...
... to acetaldehyde The latter was dehydrogenated to acetate, whereas the 3-ketopropanoate was hydrated to 3-hydroxypropanoate Fig Selected 1H- NMR spectra from propynoate metabolism (A) Spectrumof ... with a 5-mm broadband probe head For a lock a D2O vortex capillary was added to the NMR tube to avoid 1H/ D exchange reactions During measurements the tube was rotated at 20 rev.Æs )1 The huge water ... mM acetaldehyde to this biotransformation in 50% D2O caused an 10 % higher amount of hydrogen in the acetate, as it is formed directly from the acetaldehyde The incorporation ofa higher hydrogen...
... 11 (Z)- tetradecenoate to 10 (E) ,12 (E)-tetradecadienoate by initial H- abstraction at C10 and 11 (E)-tetradecenoate to 9(Z) ,11 (E)-tetradecadienoate by initial oxidative attack at C9 [39] Whether these ... and methyl esterification The overall yields of [8,8 2H2 ] -1 and [11 ,11 - 2H2 ] -1 obtained via these procedures was 5% and 14 %, respectively Purification of substrates was carried out by flash chromatography ... mL) of the pYJ/DTY1 0a2 strain of S cerevisiae incubated at 20 °C for days to permit relatively rapid growth and then at 15 °C for a further days to reach saturation at a temperature which has been...
... resonances of aliphatic side chains were achieved by 3D 15 N-resolved [ 1H, 1H] -TOCSY and 3D 15 Nresolved [ 1H, 1H] -NOESY Furthermore, homonuclear 2D [ 1H, 1H] -TOCSY and 2D [ 1H, 1H] -NOESY spectra were used to assign ... S.W., Torchia, D .A & Bax, A (19 89) Three-dimensional heteronuclear NMRof 15 N-labeled proteins J Am Chem Soc 11 1, 15 15 15 17 Bax, A. , Clore, G.M & Gronenborn, A. M (19 90) 1H- 1H correlation via isotropic ... if d is a part of the stator that links the F1 and F0 parts of the Escherichia coli ATP synthase J Biol Chem 272, 16 652 16 656 18 Bottcher, B., Schwarz, L & Graber, P (19 98) Direct indication for...
... A & Saenger, W (19 91) The refined crystal structure of a- cobratoxin 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 ˚ from Naja naja siamensis at the 2.4 A resolution J Biol Chem 266, 215 30– 215 36 ... to that of the a- cobratoxin crystal The superposition of these two ˚ structures shows an RMSD value of 3.00 A for all ˚ backbone atoms which reduces to 1. 4 A when the C-terminal tail and the tip ... [42] have found that the overall net charge of neurotoxins affects the toxin–receptor interactions Particularly, it should be noted that NTX -1 and LSIII have a net charge of+1 in comparison with...
... virus capsid protein Biochemistry 43, 9989–9998 3490 30 Matsuura K, Tamada Y, Deyashiki Y, Miyabe Y, Nakanishi M, Ohya I & Hara A (19 96) Activation of human liver alpha-hydroxysteroid dehydrogenase ... sulphobromophthalein Biochem J 313 , 17 9– 18 4 31 Noriega GO, Juknat AA & Batlle AM (19 92) Non-essential activation of rat liver porphobilinogendeaminase by folic acid Z Naturforsch [C] 47, 416 – 419 ... activation may thus be the consequence of an enhanced stability of the 66 kDa form The CT-peptide is not cleaved by enzymatically active PC1 ⁄ Another important feature of an enzymatic activator...
... fragment using the primer combination of plcrH10: 5¢-CGAGGTACATATGCAACAAGAGACG-3¢ and plcrH 11: 5¢-ACGTACAGATCTCCTTGTCGTCGTCGT CTGGGTTATCAACGCACTC-3¢ This fragment was then cloned into the expression ... nickel-nitrilotriacetic acid slurry (Qiagen) was added, followed by a 1- h incubation at °C on a rotary shaker The sample was then loaded on a Poly Prep chromatography column (Bio-Rad, CA, USA) and each subsequent ... molar ratio of : The appropriate pH was corrected by the addition of small aliquots of HCl and NaOH NMR Ó FEBS 2002 Tertiary structure of the YopD amphipathic domain (Eur J Biochem 269) 36 61 experiments...
... [36,37] The first a helix (Asp17–His33) has the characteristics of an amphipathic helix (Fig 7I) Its hydrophilic face is formed by the amino acid side chains: Asp17, Glu 21, Glu24, Glu25, Lys27, Asn28, ... 2. 6H Tyr50 3. 5H Tyr50 b1 Tyr50 b2 Tyr50 b1 Tyr50 b2 Tyr50 b1 Tyr47 b2 Tyr47 3. 5H Tyr47 3. 5H Tyr47 cH Thr55 cH Thr55 a1 Gly56 a1 Gly56 a2 Gly56 a2 Gly56 a Ala59 a Ala59 b Ala59 b Ala59 a1 Gly56 a1 ... maxima at 19 0 and 212 nm (Fig 1) This result suggests that Vpr possess a high a helical content [34,36,42] It is also important to note that TFE can stabilize a helices in regions that have already...
... on the main modifications which had been taking place overtime, both at the technical level (change of machines, aspiration and ventilation of the buildings, ) and regarding the nature ofsolvents ... solvented Adhesive Page of Workshop of the Natural Adhesive Surface: 12 9.37 m2 Number of workers: Number of stations: - Manufacture of the Natural adhesive - Conditioning of Natural adhesive Surface: ... workshop of the dissolved adhesive, in the control laboratory and in the storage halls of the finished products were greater than the TLVs (indexes are above 1; Table 4) Higher concentrations of hexane...
... AGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGACAGCAAGCGAACCGGAAT-3' GAAGTTCCTATACTTTCTAGAGAATAGGAACTTCCGGAAATGTTGAATACTCA TACTCTTCCTTTTTC-3' The linear PCR-generated fragments were electroporated into YEbac102, an E coli DH10B ... for UL8; and 18 S rRNA-f (5'-actcaacacgggaaacctca-3') and 18 S rRNA-r (5'-aaccagacaaatcgctccac-3') for 18 S rRNA Reactions were performed using SYBER Premix Ex Taq II (Takara) with the Thermal Cycler ... (B) Autoradiographic images of electrophoretically separated BamHI and HindIII digests of YK304 (lane 1) , MT102 (lane 2), and MT103 (lane 3) DNAs hybridized to the radiolabeled DNA fragment of HSV(F)...