1 detection of fields of reduced metabolism
Tài liệu Báo cáo khoa học: 1. Signal Transduction 1.1 Integration of Metabolism and Survival pdf
... REV-ERBalpha governs the circadian expression of several enzymes and regulators of lipid metabolism Our genetic Rev-Erbalpha loss -of- function and gain -of- function studies suggest that circadian ... Illnerova Department of Neurohumoral Regulations, Institute of Physiology, Academy of Sciences of the Czech Republic E-mail: sumova@biomed.cas.cz In mammals, endogenous rhythmicity of the principal ... mimicked by expression of an active form of STAT5 Epigenetic modifications of histones associated with relevant genes were delayed in response to weak agonists Addition of IL-2 failed to shorten...
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Báo cáo khoa học: Enzymes of mannitol metabolism in the human pathogenic fungusAspergillus fumigatus– kinetic properties of mannitol-1-phosphate 5-dehydrogenase and mannitol 2-dehydrogenase, and their physiological implications pot
... that, in terms of Km, AfM1PDH binds Man-ol1P two orders of magnitude more tightly than AfM2DH binds Man-ol Enzymes of mannitol metabolism in A fumigatus of $ 800 lm, AMP binds one order of magnitude ... Af M2DH Interpretation of the kinetic properties of an enzyme in the context of its role in cellular metabolism relies on knowledge of the physiological concentrations of substrates, products, ... intracellular concentration of Fru is about two orders of magnitude below the apparent Km of 40 mm A physiological role of AfM2DH in the utilization of intracellular Man-ol via reversal of path in Scheme...
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Báo cáo khoa học: Characterization of oligosaccharides from the chondroitin/dermatan sulfates 1 H-NMR and 13 C-NMR studies of reduced trisaccharides and hexasaccharides doc
... characterization of unreduced DS oligosaccharides of up to dodecasaccharide in size has been discussed [23]; a combination of 1D and 2D NMR together with electrospray MS was employed The employment of nondestructive ... chain-reducing termini by a peeling reaction, or, through the action of a tissue endo-b-d-glucuronidase A series of reduced and unreduced oligosaccharides obtained from DS were previously characterized ... region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(b1– 3)GalNAc(b1-]n, which may be O-sulfated on the C4 and ⁄ or C6 of GalNAc and C2 of...
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báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc
... Precision The precision of an analytical method describes the closeness of agreement (degree of scatter) between a series of measurements obtained from multiple sampling of the same homogenous ... (D) Flu tetr amer positive cells -CD8 FITC vs Flu tetramer PE Figure Detection of tetramer positive cells among PBMC Detection of tetramer positive cells among PBMC Gating sequence is shown in the ... insufficient number of cells Accuracy The accuracy of an analytical method describes the closeness of mean test results (detected) obtained by the method to the true value (expected) of the analyte...
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Chapter 078. Prevention and Early Detection of Cancer (Part 1) pps
... tobacco-related The degree of smoke exposure, meaning the number of cigarettes smoked per day as well as the level of inhalation of cigarette smoke, is correlated with risk of lung cancer mortality ... pediatric issue Over 80% of adult American smokers began smoking before the age of 18 Nearly 20% of Americans aged 12– 18 have smoked a cigarette in the past month Counseling of adolescents and young ... identification and manipulation of the genetic, biologic, and environmental factors in the causal pathway of cancer Education and Healthful Habits Public education on the avoidance of identified risk factors...
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Báo cáo khoa học: "Development of immunoassays for the detection of kanamycin in veterinary fields" pps
... etis-A eht ni egnahc lanoitamrofnoc lacol a secudni gnidnib nicymomoraP DJ isilguP ,S awazihsoY ,D ymruoF 054-344 ,36 ,9791 scirtaideP yduts pu-wollof dellortnoc raey-ruof a fo stluser :nicymanak ... ta nim 03 rof detabucni erew setalp eht ,yltneuqesbuS llew hcae ot dedda saw noitulos etartsbus )]dica cinoflus enilozaihtzneb lyhte-3[id-oniza-2,2( STBA lµ 001 ,gnihsaw retfA h rof esadixorep ... ydobitna gnicudorp llec amodirbyh elbats A tnatanrepus erutluc gnisu sllec gnicudorp-ydobitna rof neercs ot demrofrep saw ASILE tceridni evititepmoc ,noisuf eht retfa syad evlewT ]8[ ylsuoiverp debircsed...
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Báo cáo y học: "Association of reduced heme oxygenase-1 with excessive Toll-like receptor 4 expression in peripheral blood mononuclear cells in Behçet''''s disease" potx
... City University Center of Excellence Program of the Ministry of Education, Culture, Sports, Science and Technology of Japan (Y Ishigatsubo), Research on Specific Disease of the Health Science ... that levels of TLR4 mRNA, but not of TLR2 mRNA, are constitutively increased in PBMCs from patients with BD, regardless of disease activity The data suggest possible involvement of TLR4 in BD, ... demonstrated substantial pathologic roles of HO-1 in rheumatic diseases Abundant expression of HO-1 was identified in synovial tissues of patients with RA, in the absence of elevated serum HO-1 levels [24,25]...
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Báo cáo y học: " The intracellular detection of MIP-1beta enhances the capacity to detect IFN-gamma mediated HIV-1-specific CD8 T-cell responses in a flow cytometric setting pro" ppsx
... production of IFN-γ were rare Since the analysis of the combination of two functions decreases the background and the measurement of the IFN-γ+ MIP-1β+ T-cells was equivalent to the measurement of the ... is the method of choice for a detailed analysis of the immune response in a selected and limited number of samples In spite of an intense activity in the development and testing of new vaccines ... CD8 T-cells showed a goodness of fit of r2 = 0.9929 and a slope of 1.052 demonstrating an almost perfect linearity of the two measurements (Figure 3A) The goodness of fit was slightly lower for...
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Báo cáo y học: " Detection of HIV-1 RNA/DNA and CD4 mRNA in feces and urine from chronic HIV-1 infected subjects with and without anti-retroviral therapy" pptx
... Figure Detection of CD4 mRNA in feces collected from study participants Detection of CD4 mRNA in feces collected from study participants Representative gel picture of RT nested PCR products of CD4 ... cells with the detection limit of 2.5 copies of HIV-1 DNA/reaction HIV-1 RNA was detected from normal feces mixed with HIV-1 positive plasma with detection limit of 40 copies of HIV-1 RNA/reaction ... participants Detection of HIV-1 RNA in feces collected from study participants A: Representative gel picture of RT nested PCR products of human beta-actin mRNA B: Representative gel picture of RT nested...
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báo cáo khoa học: " Simultaneous detection of Human Immunodeficiency Virus 1 and Hepatitis B virus infections using a dual-label time-resolved fluorometric assay" potx
... Wavelength (nm) 700 750 Figure Design of the dual-label time-resolved immunofluorometric assay (A) A schematic illustration of the assay for simultaneous detection of HIV and HBV infections The Arabic ... explore the sensitivity of detection of anti-HIV1 antibodies, the dual-label assay was performed using serial dilutions (as a surrogate for a range of known concentrations) of an anti-HIV-1 antibody-containing ... sensitivity of detection of either analyte This is further borne out by the analysis of sera that contain HBsAg as well as anti-HIV1 antibodies (see below) Next, we tested the feasibility of the dual-label...
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Cytologic Detection of Urothelial Lesions - part 1 ppt
... Cytologic Detection of Urothelial Lesions With 131 Illustrations in Full Color Dorothy L Rosenthal, M.D., FIAC Professor of Pathology, Oncology, and Gynecology/Obstetrics The Johns Hopkins School of ... 21287 USA Stephen S Raab, M.D Professor of Pathology Chief of Pathology, UPMC Shadyside University of Pittsburgh Medical Center Pittsburgh, PA 15213 USA Library of Congress Cataloging-in-Publication ... organization of pathology texts The logic of decision trees, elimination of unlikely choices and narrowing of differential diagnosis via a pragmatic approach based on morphologic criteria will be some of...
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báo cáo khoa học: " Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen" pot
... fast screening of Bet v isoforms in birch pollen by determining presence and relative abundances of individual isoforms The pollen of four birch species contained a mixture of Bet v isoforms, with ... C-terminus of Arginine and Lysine Fig shows an example of the fragments I to XVII that are theoretically formed after tryptic digestion of isoforms from the subfamilies 01 to 05 Isoforms of different ... specific for both isoforms of gene 01A, while two others were specific for both isoforms of gene 01C Isoforms 02A01 and 02B01 could not be separated, so either one or both of them are expressed...
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Báo cáo khoa học: "Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe" pps
... ultrapure water The detection limit of the FQ-PCR was determined based on the highest dilution that resulted in the presence of Ct value in real-time PCR detection The detection limit of the conventional ... http://www.virologyj.com/content/7/1/37 Page of 10 Figure The sensitivity of TaqMan™ FQ-PCR detection Ten-fold serial dilutions of AHV-1 standard template were used (1-6), 1.0 × 105-1.0 × 100 copies/reaction of AHV-1 standard ... optimization of a TaqMan™ FQ-PCR Final concentrations of primers each of 0.5 μmol/L and probe of 0.25 μmol/L were selected, and the optimized annealing temperature was 53°C The combination of primers,...
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Báo cáo khoa học: " A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes" pps
... and specificity The analytical sensitivity of our LNA Taqman rRT-PCR for the detection of the HA gene of H5N1 was < 0.5 PFU of virus and 10 copies of ssDNA plasmids No fluorescence was detected ... conducted in a total volume of 25 μl containing 12.5 μl of 2× RT-PCR Reaction Mix, 400 nM of each primer, 120 nM of probe, 0.5 μl of iScript Reverse Transcriptase, and μl of template Optimized rRT-PCR ... copies of ssDNA plasmid, and less than 0.5 PFU of H5N1 viruses per reaction, and specific for the detection of influenza A of subtype H5 The HA gene of clades 1, 2.1, and 2.3 was amplified from...
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Báo cáo khoa học:" Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1?" pdf
... AHV-1 detection, of which the polymerase chain reaction (PCR) is a powerful tool with exquisite sensitivity for detection of minute amounts of nucleic acids, even against a high background of unrelated ... that of AHV-1 detection From the ratio of the calculated amount of IC to the actual amount of IC, which is shared by the specimen, the normalization could be achieved and the actual amount of AHV-1 ... for testing large numbers of samples since the FQ-PCR presented the benefits of increased speed due to reduced cycle time and remove of post-amplification process, offering considerable labor...
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Báo cáo khoa học: " Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1" pdf
... AHV-1 detection, of which the polymerase chain reaction (PCR) is a powerful tool with exquisite sensitivity for detection of minute amounts of nucleic acids, even against a high background of unrelated ... that of AHV-1 detection From the ratio of the calculated amount of IC to the actual amount of IC, which is shared by the specimen, the normalization could be achieved and the actual amount of AHV-1 ... for testing large numbers of samples since the FQ-PCR presented the benefits of increased speed due to reduced cycle time and remove of post-amplification process, offering considerable labor...
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Báo cáo y học: " Modulation of HIV-1 macrophage-tropism among R5 envelopes occurs before detection of neutralizing antibodies" pdf
... protection of these CD4 contact residues from antibodies may compromise the affinity of envelope for CD4 and in turn restrict tropism to cells expressing higher levels of CD4 (e.g T-cells) In support of ... Retrovirology 2010, 7:48 http://www.retrovirology.com/content/7/1/48 Page of Figure Detection of neutralizing antibodies present in the plasma of subjects who yielded gp120 sequences studied here Serially ... as: Richards et al., Modulation of HIV-1 macrophage-tropism among R5 envelopes occurs before detection of neutralizing antibodies Retrovirology 2010, 7:48 Page of ...
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Báo cáo y học: "Comparison of a Two-Lead, Computerized, Resting ECG Signal Analysis Device, the MultiFunction-CardioGramsm or MCG (a.k.a. 3DMP), to Quantitative Coronary Angiography for the Detection of Relevant Coronary Artery Stenosis (70%)
... large inventory of normalized mathematical indexes of abnormality It is the pattern of these mathematical indexes of abnormality, obtained from analysis of multiple cardiac cycles of the resting ... assess the performance of the prediction of stenosis independent of the prevalence of stenosis, the positive and negative likelihood ratios (LR) were calculated [24] A value of p
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Lab 1: Game of Life
... make use of the get next state(x,y) function to compute the next state of each cell (d) Write the code for get next state(x,y), so the function returns the next state (ALIVE or DEAD) of the cell ... using the number of live neighbors (returned by the num neighbors(x,y) function) and the Game of Life rules (e) Fill in the function num neighbors(x,y), so it returns the number of live neighbors ... off: Part B: The World in a File In the first part of this lab, the initial state of the world was hard-coded into lifegame.c and the final state of the world was output to the console In this part,...
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