antithrombotic mechanisms of the endothelial cell surface endothelial cells express glycosaminoglycan gag molecules containing heparan sulfate to which thrombin and antithrombin can bind they also express thrombomodulin tm and the endoth
Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx
... a virus and the cell surface The options to use unpurified viruses, low MOI and cell numbers indicate the opportunity to study virus host cell interaction even when the amount of cells and viruses ... specific binding to HeLa and recombinant CHO-DAF cells were statistical significant compared to CHO cells The relative binding (fold difference) of viruses attached to HeLa in comparison to CHO ... B) MOI 0.05 TCID50 /cell of the labeled, purified virus and C) MOI 0.05 TCID50 /cell of unpurified virus incubated as described in A) and the amount of virus bound to the cell surface was measured...
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Báo cáo y học: " Six host range variants of the xenotropic/polytropic gammaretroviruses define determinants for entry in the XPR1 cell surface receptor" ppt
... Comparison of the deduced amino acid sequences of the ECL3 and ECL4 domains of the Xpr1 genes of rodents and mink Comparison of the deduced amino acid sequences of the ECL3 and ECL4 domains of the Xpr1 ... cells carrying Xpr1c and on Rat2 cells, and is restricted by hamster cells and cells carrying the mouse Xpr1n and Xpr1p variants XMVs: a host range variant defined by AKR6 and XMRV Three of the ... by their failure to infect cells of the laboratory mouse; AKR6 and the human derived XMRV, have XMV infectivity patterns on mouse cells, but resemble PMVs in their inability to infect hamster cells...
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Analysis of the mechanical reaction of human endothelial cells to a single constant or transient uniaxial strain
... of endothelial cells to uniaxial strains is of primary interest in this work The cells are able to alter their internal stresses and forces they apply in order to withstand mechanical loads Cell ... Among the assortment of adherent cells, vascular endothelial cells (VECs) are of great interest, because they naturally undergo a variety of mechanical stresses (Figure 1.1) Smooth muscle cells ... work the mechanical response of vascular endothelial cells was studied These cells are known to be responsive to mechanical stimuli present in their physiological environment [2], where they...
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Báo cáo khoa học: Molecular mechanisms of the phospho-dependent prolyl cis ⁄ trans isomerase Pin1 docx
... position, and Pin1 can restore this function [40] Understanding the binding of Tau to tubulin and its modulation by phosphorylation will be necessary before we can evaluate the role of Pin1 in ... preliminary binding of the WW domain to the pSer2-Pro motif and subsequent isomerization by the catalytic domain at the Ser5 position The yeast Ess1 protein, however, prefers both to bind and isomerize ... redundancy of protein copy numbers in the cell, at least under normal growth conditions Indeed, they found that although wild-type yeast cells contain on the order of 200 000 molecules of Ess1 per cell, ...
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Báo cáo khoa học: Restriction point control of the mammalian cell cycle via the cyclin E/Cdk2:p27 complex doc
... sufficient, mitogen containing culture medium Once the cell has transgressed the RP, the necessity of a mitogen (i.e growth factor) containing medium is relieved and cells commit to replicate their DNA ... (S-phase) and complete the remainder of the division cycle autonomously [2,3] Cells in the G1phase that have not yet passed RP, monitor their environment and own size to determine whether they are ... of the RP has been linked to several disease states, most notoriously cancer [1,4], and quantification of the contribution of the different reaction steps in the cell cycle to the control of the...
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Báo cáo khoa học: ERK and cell death: Mechanisms of ERK-induced cell death – apoptosis, autophagy and senescence doc
... neighboring cells The intrinsic pathway of apoptosis regulates the activity of the Bcl-2 family proteins that control the integrity of the mitochondrial membrane The release of proapoptotic factors ... evaluation of the role of insulinlike growth factors (IGF) and of type-I IGF receptor signalling in hepatocarcinogenesis and in the resistance of hepatocarcinoma cells against drug-induced apoptosis ... cytoplasmic death domain -containing protein that binds and sequesters ERK, is highly expressed in the cytoplasm of Sertoli cells, spermatogonia and spermatocytes, inducing a cytoplasmic ERK localization...
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Báo cáo khoa học: A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea docx
... concentration of urea in the absence and presence of TMAO The Dm values for the urea-induced unfolding of FtsZ were found to be 1.8 and 3.6 m in the absence and presence of TMAO, respectively Furthermore, ... analysis of the FtsZ assembly reaction showed the formation of thicker and larger bundles of FtsZ protofilaments in the presence of TMAO compared with the control (Fig 6) The widths of the bundles of ... reduction in the GTPase activity of FtsZ in the presence of TMAO may be partly due to the solvophobic effects of TMAO on FtsZ that reduces the binding of GTP to FtsZ TMAO enhanced aggregation of FtsZ...
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Báo cáo khoa học: Mitochondrial connection to the origin of the eukaryotic cell pdf
... there can be no appropriate molecular tracers for the origin of the nucleus, endomembrane, and cytoskeleton Nonetheless, phylogenetic methods can still be applied to proteins, the appearance of which ... shown to be imported into mitochondria of diatoms and oomycetes Notwithstanding the sister relationship of c proteobacteria and Eukarya, these data were interpreted as evidence for the mitochondrial ... synthetases, which lack the insert, revealed the presence of the insert to be a derived state The authors interpreted these data as evidence for acquisition of ValRS by eukaryotes from the mitochondrial...
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university of california press fundamentals of the stem cell debate the scientific religious ethical and political issues dec 2007
... is the source of hematopoietic stem cells This wellunderstood stem cell type gives rise to red blood cells and platelets as well as white blood cells (B cells and T cells) that function in the ... monocytes, and platelets (figure 7) The bone marrow also produces all of the cells of the immune system, including B cells for the circulation and the lymph nodes and spleen; T cells for the thymus; and ... with respect to the origin of the stem cells It is often very difficult to determine whether the stem cells are authentic components of the organ system where they are found or cells that have...
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A review of the chemical and physical mechanisms of the storage stability of fast pyrolysis bio oils
... content of the feedstock • The heat transfer rate and final char temperature during pyrolysis • The extent of vapor dilution in the reactor • The time and temperature history of the vapors in the ... • The water content of the bio-oil • The extent of contamination of the bio-oil during storage by corrosion or leaching of the containers • Exposure to air during storage • The length of storage ... in the reactor • The time and temperature history of the vapors in the heated transfer lines from the pyrolysis reactor through the char removal equipment to the quench zone • Whether the vapors...
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Báo cáo hóa học: "Effect of the carbon nanotube surface characteristics on the conductivity and dielectric constant of carbon nanotube/poly(vinylidene fluoride) composites" doc
... due to the reduction of the surface conductivity of the CNTs due to the oxidation process [8], and is similar for all the functionalized composites Further, the increase of surface area due to the ... CNTs The reduction of the electrical surface conductivity of the CNT due to the oxidation process, together with an increase of the surface area and defect formation, is at the origin of the observed ... desorption (TPD) and determination of pH at the point of zero charge (pHPZC) from acid-base titration according to the method of the literature [11] The total amounts of CO and CO evolved from the samples...
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Báo cáo sinh học: "Growth control of the eukaryote cell: a systems biology study in yeas" ppt
... genomic level to the cell s phenotype [36,37], gas chromatography coupled to time -of- flight mass spectrometry (GC/TOF-MS) was used to analyze the complement of intracellular and extracellular metabolites, ... decided to test the generality of the hypothesis that TOR, more specifically the TOR signaling branch that mediates temporal control of cell growth (TORC1) complex [32], is the major regulator of ... biosynthesis in yeast Trends Biochem Sci 1999, 24:437-440 Fingar DC, Blenis J: Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell...
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Báo cáo y học: " Ageing, autoimmunity and arthritis: Senescence of the B cell compartment — implications for humoral immunity" pptx
... chains and Ig-α/β marks the transition to the immature B cell stage Immature B cells are the earliest cells in the lineage that express a bona fide antigen specific B cell receptor (BCR), and therefore ... B cells not decline with age, maintaining that the major defect in B cell development of old mice is the inability of newly made cells to join the peripheral B cell compartment.’ They hypothesize ... defective in their ability to reconstitute the B cell compartment, but they retained their ability to reconstitute both the T cell and myeloid compartments effectively From these data, the authors...
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Báo cáo y học: "A model of anti-angiogenesis: differential transcriptosome profiling of microvascular endothelial cells from diffuse systemic sclerosis patients" potx
... down-regulated in SSc endothelial cells We observed that SSc endothelial cells overexpress pro-angiogenic and anti-angiogenic transcripts, and down-regulation of genes critical to cell migration and proliferation ... serum, and 20 µg/ml endothelial cells growth supplement N-MVEC spontaneously form anastomosing cords of cells resembling a capillary plexus, which are well organized by hours The process of endothelial ... were added to both the upper and lower compartment of the migration chamber Irrelevant mouse IgGs were used to verify the specificity of the effect The number of cells moving across the filter...
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Báo cáo y học: "Genome-wide analysis of the diatom cell cycle unveils a novel type of cyclins involved in environmental signaling" pptx
... On the other hand, the wide variety of dsCYC genes in diatoms expressed early during the cell cycle renders them plausible candidates to fulfil the task of signal integrators Moreover, diatom-specific ... 4C) of the cells during the 24-h time series (b) Expression profiles of early cell cycle genes (c) Expression profiles of late cell cycle genes Error bars represent standard errors of the mean of ... 12-h time course of synchronized cells in the presence of nocodazole At the later time points, an increasing enrichment of 4C cells can be observed because of a blockage of the cells at metaphase...
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Báo cáo y học: " High-resolution transcription atlas of the mitotic cell cycle in budding yeast" pptx
... Additional file for the determination of the boundaries of the cell cycle phases) Overall, the peak times of antisense periodic expression were consistent with the waves of expression of periodic proteincoding ... expression atlas of the budding yeast cell cycle is thus a resource with which to unravel a potential additional level of the cell cycle regulatory circuit, as well as to study the periodic expression ... readers (TECAN) Additional file 1: A table providing control data on the synchronous division of the yeast cells Excel sheet contains a table of the number and percentage of budded cells and dividing...
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Báo cáo y học: "Anti-Inflammatory mechanisms of the proteinaseactivated receptor 2-inhibiting peptide in human synovial cells" ppt
... normalized to the amount of GAPDH The mean and standard deviation were used to evaluate COX-2 and MMP-1 expression levels Student’s t-test was used for the comparison The effects of stimulation ... However, the role of PAR-2 in synovial cells has not been well investigated Therefore in the present study, we investigated the effects of PAR-2 activation and inhibition of COX-2 and MMP-1 expressions ... synovial cells as well as in model cells, which suggested anti-inflammatory mechanisms of the PAR2-IP Trypsin is well recognized as an activator of PAR2 Importantly, trypsin was able to mimic...
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báo cáo khoa học: "Determination of the volume-specific surface area by using transmission electron tomography for characterization and definition of nanomaterials" docx
... From the isosurface based volume rendering of the ET reconstructions, the total surface area and volume of their composing gold particles could be measured For example, the total surface area and ... the size range of 1-100 nm can be shown From the 3D reconstructions, the surface area and the volume of the examined NM could be estimated directly and the VSSA was calculated The mean VSSA of ... area and volume can be closely approximated by the formulas to calculate the surface area and the volume of a perfect sphere Figure 1C and 1D show the correlations between the calculated and measured...
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Báo cáo y học: " Successful long-term monotherapy with rituximab in a patient with chronic lymphocytic leukemia of the B-cell-lineage: a case report" pdf
... bronchoscopy and blood were repeatedly negative Considering the remaining therapeutic options and the risks of any further aggressive chemotherapy in a severely pancytopenic patient, rituximab monotherapy ... line therapy The complete lack of response to chlorambucil in our patient suggests an aggressive course of the disease, which was indeed documented by the rapid progression and deterioration of ... consequence of the secondary humoral immune deficiency in CLL, the frequency and severity of the infections in our patient were certainly due to a combined humoral and cellular immune defect The severity...
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Báo cáo khoa học: " Mechanisms of the action of povidone-iodine against human and avian influenza A viruses: its effects on hemagglutination and sialidase activities" doc
... by PVP-I of influenza A virus growth in MDCK cells We first determined the cytotoxicity of PVP-I against MDCK cells employed as host cells of influenza viruses in this study by using a cell counting ... cytotoxic concentration of PVP-I after 24-h exposure of MDCK cells to PVP-I was 2.4 ± 0.2 mg/ml PVP-I ranging from 0–1.56 mg/ml, which had no effect on MDCK cells, reduced virus yield in MDCK cells ... surface of the culture plate after treatment with 3.1 mg/ml of PVP-I suggested that the cells were undergoing apoptosis Therefore, we used low concentrations of PVP-I that did not cause any toxicity...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25